LOCUS       NM_001040395            3674 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus NAD kinase 2, mitochondrial (Nadk2), transcript
            variant 2, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001040395 XR_001575 XR_004512 XR_004513 XR_004514 XR_004515
            XR_005140
VERSION     NM_001040395.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Sang,R., Wei,J., Pan,J., Yang,R., Mao,L. and Bao,J.
  TITLE     [Coxsackie virus B3 (CVB3) triggers the activation of NLRP3 in
            mouse macrophages by activating nicotinamide adenine dinucleotide
            kinase 2 (NADK2)]
  JOURNAL   Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 39 (1), 34-40 (2023)
   PUBMED   36631013
  REMARK    GeneRIF: [Coxsackie virus B3 (CVB3) triggers the activation of
            NLRP3 in mouse macrophages by activating nicotinamide adenine
            dinucleotide kinase 2 (NADK2)].
REFERENCE   3  (bases 1 to 3674)
  AUTHORS   Murray,G.C., Bais,P., Hatton,C.L., Tadenev,A.L.D., Hoffmann,B.R.,
            Stodola,T.J., Morelli,K.H., Pratt,S.L., Schroeder,D., Doty,R.,
            Fiehn,O., John,S.W.M., Bult,C.J., Cox,G.A. and Burgess,R.W.
  TITLE     Mouse models of NADK2 deficiency analyzed for metabolic and gene
            expression changes to elucidate pathophysiology
  JOURNAL   Hum Mol Genet 31 (23), 4055-4074 (2022)
   PUBMED   35796562
  REMARK    GeneRIF: Mouse models of NADK2 deficiency analyzed for metabolic
            and gene expression changes to elucidate pathophysiology.
REFERENCE   4  (bases 1 to 3674)
  AUTHORS   Kim,H., Fu,Z., Yang,Z., Song,Z., Shamsa,E.H., Yumnamcha,T., Sun,S.,
            Liu,W., Ibrahim,A.S., Qi,N.R., Zhang,R. and Zhang,K.
  TITLE     The mitochondrial NAD kinase functions as a major metabolic
            regulator upon increased energy demand
  JOURNAL   Mol Metab 64, 101562 (2022)
   PUBMED   35944895
  REMARK    GeneRIF: Deleting MNADK (NADK2) in mice reduced fat oxidation upon
            energy demand triggered by endurance exercise or fasting. NADK2 is
            a major metabolic regulator upon increased energy demand.
REFERENCE   5  (bases 1 to 3674)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3674)
  AUTHORS   Perez-Garcia,V., Fineberg,E., Wilson,R., Murray,A., Mazzeo,C.I.,
            Tudor,C., Sienerth,A., White,J.K., Tuck,E., Ryder,E.J., Gleeson,D.,
            Siragher,E., Wardle-Jones,H., Staudt,N., Wali,N., Collins,J.,
            Geyer,S., Busch-Nentwich,E.M., Galli,A., Smith,J.C., Robertson,E.,
            Adams,D.J., Weninger,W.J., Mohun,T. and Hemberger,M.
  TITLE     Placentation defects are highly prevalent in embryonic lethal mouse
            mutants
  JOURNAL   Nature 555 (7697), 463-468 (2018)
   PUBMED   29539633
REFERENCE   7  (bases 1 to 3674)
  AUTHORS   Zhang,K., Kim,H., Fu,Z., Qiu,Y., Yang,Z., Wang,J., Zhang,D.,
            Tong,X., Yin,L., Li,J., Wu,J., Qi,N.R., Houten,S.M. and Zhang,R.
  TITLE     Deficiency of the Mitochondrial NAD Kinase Causes Stress-Induced
            Hepatic Steatosis in Mice
  JOURNAL   Gastroenterology 154 (1), 224-237 (2018)
   PUBMED   28923496
REFERENCE   8  (bases 1 to 3674)
  AUTHORS   Zhang,R.
  TITLE     MNADK, a novel liver-enriched mitochondrion-localized NAD kinase
  JOURNAL   Biol Open 2 (4), 432-438 (2013)
   PUBMED   23616928
  REMARK    GeneRIF: MNADK is localized to mitochondria in Hep G2 cells, and a
            recombinant MNADK showed NAD kinase activity. MNADK is abundant in
            mitochondrion-rich tissues, such as liver. MNADK expression was
            suppressed in human liver tumors. Identification of MNADK
            immediately suggests a model in which NADK and MNADK are
            responsible for de novo synthesis of NADP(+) in cytosol and
            mitochondria, respectively.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 3674)
  AUTHORS   Lumayag,S., Haldin,C.E., Corbett,N.J., Wahlin,K.J., Cowan,C.,
            Turturro,S., Larsen,P.E., Kovacs,B., Witmer,P.D., Valle,D.,
            Zack,D.J., Nicholson,D.A. and Xu,S.
  TITLE     Inactivation of the microRNA-183/96/182 cluster results in
            syndromic retinal degeneration
  JOURNAL   Proc Natl Acad Sci U S A 110 (6), E507-E516 (2013)
   PUBMED   23341629
REFERENCE   10 (bases 1 to 3674)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK046666.1, AK078318.1, AK162601.1, AW537715.1, BE692075.1 and
            BY545448.1.
            
            On Nov 1, 2013 this sequence version replaced NM_001040395.3.
            
            Transcript Variant: This variant (2) lacks an in-frame exon in the
            central coding region, compared to variant 1. The encoded isoform
            (2) is shorter, compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK162601.1, SRR7652917.697901.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-398               AK046666.1         2-399
            399-1066            AK078318.1         2-669
            1067-1837           AK078318.1         736-1506
            1838-3038           AK162601.1         1412-2612
            3039-3577           AW537715.1         51-589
            3578-3665           BE692075.1         8-95                c
            3666-3674           BY545448.1         44-52
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 3.82 cM"
     gene            1..3674
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /note="NAD kinase 2, mitochondrial"
                     /db_xref="GeneID:68646"
                     /db_xref="MGI:MGI:1915896"
     exon            1..506
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            507..595
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            596..684
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            685..766
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            767..850
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            851..987
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            988..1066
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1067..1162
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..1218
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1219..1272
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1396
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..3674
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1805..1810
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /note="hexamer: AATAAA"
     polyA_site      1834
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /note="major polyA site"
     regulatory      3324..3329
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /note="hexamer: ATTAAA"
     polyA_site      3345
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /note="major polyA site"
     regulatory      3645..3650
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
                     /note="hexamer: AGTAAA"
     polyA_site      3666
                     /gene="Nadk2"
                     /gene_synonym="MNADK; Nadkd1"
ORIGIN      
        1 gagaggccac tgcgcaggcg cccaagaaaa cggataaaca aacaaataaa acccactgcc
       61 cgggggccag cccggatccc cgcctcccgg ggcctcccgg ggcctctccg ccctcgcgtg
      121 cgtctgggca cgtccccccc ccccccggcg cgttccacgc acgtccccgc acttaacctg
      181 tgcgcggccg gcgcggtgct ggcggcaggg agcgcgagcc tgcacccgcg gccccgcgga
      241 tcatgacttg ctaccggggc ttcctgctgg gcagctgccg gcgcgtggcg ggtggccgcg
      301 cggctctgcg gggttcgggc tcgggcgcgg acggccggcg gcacttgggg cacgggcagc
      361 cgcgcgagct ggcgggcggt ggtagccccg ccgacggcgg cttccgcccc tcgcgggtgg
      421 tggtggtggc gaaaaccacc cgctacgagt tcgagcagca gcggtaccgc tacgcggagc
      481 tctcggagga ggacctgaag cagctgcttg cattgaaagg ctcgagctac agtggactac
      541 ttgagcgaca tcatattcac accaaaaatg tagaacacat aattgatagt ttacgggatg
      601 aagggattga ggttcgcctg gtcaagcgga gggagtacga tgaagagaca gttcgatggg
      661 cagacgctgt catagctgcg ggaggtgatg gcacgatgct tctggctgcg agtaaagtcc
      721 tggacagact taaacctgtc attggggtca acactgatcc ggaacggtct gaaggtcact
      781 tatgtctgcc tgtgcgatac acgcattcct tccctgaagc cttacggagg ttctctcgtg
      841 gtgagttcag gtggttgtgg aggcagcgga tcaggttata tctggaaggg actggcataa
      901 accccactcc cgtggacctt cacgaacagc agctgagctt gaatcagcac agccgagcct
      961 ttaacatcga aagagcgcac gatgaaaggt cggaggcgtc agggcctcag cttctgccgg
     1021 tgagagcgct caatgaagtc ttcattgggg agagcctgtc atccagggct tcctactatg
     1081 aaatttcagt cgatgacggc ccatgggaga agcagaagag ctcagggctc aatttgtgca
     1141 ctgggacagg atcgaaggcc tggtcattca atattaacag ggttgcagcg caggctgttg
     1201 aagatgtttt acatattgca agacgacaag gaaatctgac tcttccattg aacaaagact
     1261 tggtagaaaa agtgacaaac gaatataacg aatcattgct ctacagtcca gaagaaccaa
     1321 aaatactttt cagtattcga gagccaatag caaacagagt tttctccagc agccgtcagc
     1381 gttgtttctc ttccaaagtt tgtgttcgtt ctcgatgttg ggatgcctgc atggttgtgg
     1441 atggaggaac gtcttttgag tttaacgacg gagcaattgc ttcaatgatg atcaataaag
     1501 aagatgagct tcgaactgtg attctagagc agtgaagggc gtcctcaggc gctgcatgcg
     1561 attagtaggc atttttttct gcagatacag atgaccagcc atgcaccgac cttgttagga
     1621 ttcttaatga caggctacca ctagagtcaa ggtgacaaaa tgggacgcgt gtgctctcct
     1681 gctgattctg acagacaaga gctgttcacc gtgagacgga ctaaatggac tagaattatg
     1741 tcaaagcttc tgtattattt acagttggta accaagagca ttgatactct ttagaggcag
     1801 gttaaataaa ggattaagta tttaggattt gaaatttaac tgtacaagtg ttgttttctt
     1861 cctaactttg tggaattggc catgtgtatc agttgattag tacattattt ttatagtact
     1921 taaaagtaaa gatttaaaaa aatttttgat gatggagaat tttgataaat cctgatgttg
     1981 acttaactga agttggtaaa tgtgttttct atagctaaga attttctaat tctaggaaac
     2041 catgatggaa tcactattga ttgaaaccat gggaacagat tatttgtaga caaattctga
     2101 cttgttttgg gtgtcatcag tgctagatga aacacatcta tgggacatgt cattgacact
     2161 ttgtaaagta ttaatttcca tagcaattat tgttcagaca agtaactgag tccgatagcc
     2221 cctttcctga ttttggtttt ggatttctat tatgaaatca aggattagaa ggataatcaa
     2281 tgattgtgct ctgttcagta aaatgttcaa agacccttcc tttagtattg ggcatggact
     2341 ctagtaaaca cctgggactt aagacagaat tttggaattg ccttaaatta gtattgtgat
     2401 ttccatttcc tgtattctaa aatttaaaca atagctttgc gggatgaaca gcttccccat
     2461 cttccggtat gaaaaccact cttagttttg gagaaaacta ttttaattct tgattttgtt
     2521 ttcatgaaaa gcttctccat tgctagataa tggatggtat tttcatgctg ggtttttcag
     2581 acttgtgcat tttcagttgt tggtgaactt ccttttttat tgacaatatg aagactcatt
     2641 ctgtgccatc taccatataa gtatatatct gtatcatgtt atagatatat atgatataat
     2701 gaattgttgc atagacatcg aaatagaccg tttatggaac ttgagcttcg tgagctcaga
     2761 agtaaaaaac taaagctagg atgtatgatg agatcaaatg ctcttagctt tgccaaacgc
     2821 aacacgctgc tctttaacaa ttaagaaaaa tgaatgtcac tgtgtgaggt aatttagatc
     2881 tgtctgggtt tgtgtgttta tttagaacat ttcccttgac tatagaagga aataagtgtt
     2941 tttatctgag ccaactctaa tttctagttt aacttttttt ttttttttaa atggatatat
     3001 ttaagatatc cctttatatg aaagttaaat accttgttag aaaaaaaatg ccactctcat
     3061 ctttaatgta cctagatctg gttgtccatg atttaccaca catctattta tacgtaccac
     3121 taattggtaa tgagctgtgt gaaatcgttc ttaaactacc cttggaaagg tattaaaatt
     3181 acttattatg ctctgcctac aatctaactt ctactctgtg gcaggcaggg atttatttgt
     3241 aatagactaa tggcaaagga tgttggaata tcaaaaatgt ctttaaatgg gttaaccttt
     3301 aatgaccact ttatgcaaat aaaattaaac ctttgtccat ttttactttg ttttcttatt
     3361 tcctaaattc ttctgaaatc actacataaa agcccgagtt ttcccctgaa tactatgttt
     3421 caagtcttta tccccacagg catgtgattc tgtatactgt agacttagtt cattgccatt
     3481 atgtggcaga gtgattatgt gaatctgtct attcagagga cagaatgatt aattcaaaaa
     3541 tgtatttttc atataactta gttcagtatt tttgatgttc cattatgttt taatattctt
     3601 gaatggatag cttttgtata acaaaacaat ttcattttta tgtaagtaaa ataacaaata
     3661 attatattca cttg
//
