LOCUS       NM_028279               3868 bp    mRNA    linear   ROD 23-APR-2024
DEFINITION  Mus musculus N-acetylated alpha-linked acidic dipeptidase 2
            (Naalad2), transcript variant 3, mRNA.
ACCESSION   NM_028279
VERSION     NM_028279.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Sedlak,F., Kvasnicka,A., Maresova,B., Brumarova,R., Dobesova,D.,
            Dostalova,K., Sramkova,K., Pehr,M., Sacha,P., Friedecky,D. and
            Konvalinka,J.
  TITLE     Parallel Metabolomics and Lipidomics of a PSMA/GCPII Deficient
            Mouse Model Reveal Alteration of NAAG Levels and Brain Lipid
            Composition
  JOURNAL   ACS Chem Neurosci 15 (7), 1342-1355 (2024)
   PUBMED   38377674
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Ji,T., Pang,Y., Cheng,M., Wang,R., Chen,X., Zhang,C., Liu,M.,
            Zhang,J. and Zhong,C.
  TITLE     Deletion of glutamate carboxypeptidase II (GCPII), but not GCPIII,
            provided long-term benefits in mice with traumatic brain injury
  JOURNAL   CNS Neurosci Ther 29 (12), 3786-3801 (2023)
   PUBMED   37349952
  REMARK    GeneRIF: Deletion of glutamate carboxypeptidase II (GCPII), but not
            GCPIII, provided long-term benefits in mice with traumatic brain
            injury.
REFERENCE   3  (bases 1 to 3868)
  AUTHORS   Collard,F., Vertommen,D., Constantinescu,S., Buts,L. and Van
            Schaftingen,E.
  TITLE     Molecular identification of beta-citrylglutamate hydrolase as
            glutamate carboxypeptidase 3
  JOURNAL   J Biol Chem 286 (44), 38220-38230 (2011)
   PUBMED   21908619
  REMARK    GeneRIF: analysis of GCP2 and GCP3; beta-citrylglutamate hydrolase
            is glutamate carboxypeptidase 3
REFERENCE   4  (bases 1 to 3868)
  AUTHORS   Bzdega,T., Crowe,S.L., Ramadan,E.R., Sciarretta,K.H.,
            Olszewski,R.T., Ojeifo,O.A., Rafalski,V.A., Wroblewska,B. and
            Neale,J.H.
  TITLE     The cloning and characterization of a second brain enzyme with NAAG
            peptidase activity
  JOURNAL   J Neurochem 89 (3), 627-635 (2004)
   PUBMED   15086519
  REMARK    GeneRIF: cloning and characterization of a mouse enzyme that is
            homologous to an enzyme identified in a human lung carcinoma.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK133611.1 and AC154699.2.
            
            [WARNING] On Aug 6, 2024 this sequence was replaced by NM_028279.5.
            
            On Oct 26, 2017 this sequence version replaced NM_028279.3.
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (b) is
            shorter at the N-terminus compared to isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK133611.1, AY243507.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2713              AK133611.1         3-2715
            2714-3868           AC154699.2         45348-46502
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 7.02 cM"
     gene            1..3868
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /note="N-acetylated alpha-linked acidic dipeptidase 2"
                     /db_xref="GeneID:72560"
                     /db_xref="MGI:MGI:1919810"
     exon            1..244
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     CDS             163..2385
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /EC_number="3.4.17.21"
                     /note="isoform b is encoded by transcript variant 3;
                     glutamate carboxypeptidase III; NAAG-peptidase II;
                     NAALADase II; N-acetylaspartylglutamate peptidase II;
                     N-acetylated-alpha-linked acidic dipeptidase II"
                     /codon_start=1
                     /product="N-acetylated-alpha-linked acidic dipeptidase 2
                     isoform b"
                     /protein_id="NP_082555.2"
                     /db_xref="CCDS:CCDS52728.1"
                     /db_xref="GeneID:72560"
                     /db_xref="MGI:MGI:1919810"
                     /translation="MARPRHLRGLGMCITAVLASFIAGFTVGWFIKPLKETTTSAGYH
                     QSIQQKLLSEMKAENIRSFLRSFTKLPHLAGTEQNLLLAKKIQTQWKKFGLDSANLVH
                     YDVLLSYPNETNANYVSIVDEHGVEIFKTSYLEPPPDGYENVTNIIPPYNAFSASGMP
                     EGELVYVNYARTEDFFKLEREMNINCTGKIVIARYGKIFRGNKVKNAMLAGAMGIILY
                     SDPADYFAPDVQPYPKGWNLPGAAAQRGNVLNLNGAGDPLTPGYPAKEYTFRLPVEEA
                     VGIPNIPVHPIGYNDAERLLRNLGGAAPPDKSWKGSLNVSYNIGPGFTGSEYSRNIRM
                     HVNNINKITRIYNVIGTIRGSTEPDRYVILGGHRDSWVFGGIDPTTGTAVLQEIARSF
                     GKLVNGGWRPRRTIIFASWDAEEFGLLGSTEWAEENAKLLQERSIAYINSDSAIEGNY
                     TLRVDCTPLLNQLVYKVAREISSPDDGFESKSLYESWLEKDPSPENKECPRINKLGSG
                     SDFEAYFQRLGIASGRARYTKNKKTDKYSSYPVYHTIYETFELVQNFYDPTFKKQLSV
                     AQLRGALVYELADSVVIPFNIQDYAKALKNYAASIFNISKKHDQQLRNHAVSFDPLFS
                     AVKNFSEAASDFHRRLTQVDLNNPIAVRIMNDQQMLLERAFIDPLGLPGRKFYRHIIF
                     APSSHNKYAGESFPGIYDAMFDIENKADPSLAWAEVKKHISIAAFTIQAAAGTLTNVL
                     "
     exon            245..356
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            357..543
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            544..645
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            646..771
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            772..958
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            959..1052
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1151
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1237
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1357
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1358..1440
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1504
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1505..1572
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1573..1664
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1755
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            1756..2020
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2021..2102
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2103..2195
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..3868
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2560
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
     regulatory      2778..2783
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /note="hexamer: AATAAA"
     polyA_site      2799
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /note="major polyA site"
     regulatory      3842..3847
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
                     /note="hexamer: AATAAA"
     polyA_site      3863
                     /gene="Naalad2"
                     /gene_synonym="2700022G20Rik; D9Ertd285e; Folh1b; GCP3;
                     GCPIII; NAADALASE2; NAALADASE2"
ORIGIN      
        1 agactcaaca ggaagcagcc tgctcccccg gggcttccaa ctcctctcta gagtttccct
       61 tgtctctgga tctttgcaaa attcctcttc tcatcttagc tatccatctg cagcccccag
      121 gcctgcagtt gcagtcagag gtactccctg acccaaaaag gcatggcaag gcctaggcat
      181 ctccgtggct tgggaatgtg cattaccgcg gtgctggcct ccttcatcgc tggctttact
      241 gtgggctggt ttattaagcc tctcaaagaa acaactactt ctgctggtta tcatcaaagt
      301 atacaacaga aactcctatc tgaaatgaaa gctgaaaaca ttagatcatt tcttcgttcc
      361 tttacaaaac ttccccatct ggcaggaacg gaacaaaatt tacttcttgc caagaaaatc
      421 caaacacagt ggaagaaatt tggattagat tcagctaacc tggttcatta cgatgttctc
      481 ttgtcttatc caaatgagac caatgcaaac tatgtatcca ttgtggatga gcatggagtt
      541 gagattttta aaacatcata ccttgaacca ccaccagatg gatatgagaa tgttacaaac
      601 atcataccac catacaatgc gttttcagcc agcggcatgc cggagggaga gcttgtgtat
      661 gtcaactacg ctcgtactga ggacttcttc aaactagaaa gagaaatgaa catcaactgc
      721 actggcaaga ttgttatcgc aagatatggg aagatcttta gaggaaacaa agtcaaaaat
      781 gccatgctag ctggggccat gggaatcatt ctgtactctg atccagctga ctactttgcc
      841 cctgatgtgc agccatatcc caaaggatgg aaccttcctg gagctgcagc tcagagagga
      901 aatgtcttaa atctgaatgg ggcaggtgac ccactcactc caggctatcc agctaaagaa
      961 tacaccttca gacttcctgt tgaagaagca gtggggattc caaacatacc tgtacatcct
     1021 attggatata atgatgcaga gagactatta cgcaacttgg gaggagctgc tccaccagac
     1081 aaaagctgga agggaagtct aaatgtcagt tacaatattg ggcctggctt tacaggaagt
     1141 gaatattcca ggaacattag aatgcatgtt aataacatta acaaaatcac aagaatttac
     1201 aatgtaattg gaactatcag agggtctaca gaacctgaca ggtatgtcat tctcggaggt
     1261 catcgtgact cctgggtgtt tggagggatt gaccctacca ctgggacagc agttttgcaa
     1321 gagattgcgc gaagttttgg gaaacttgtg aatggaggtt ggagacctag gagaactatc
     1381 atttttgcca gctgggatgc agaggagttt gggctgctgg ggtcaactga atgggctgaa
     1441 gagaatgcaa agctcctaca ggagagaagc attgcttaca tcaactcaga ttctgctata
     1501 gaaggtaatt acactcttcg agttgattgt actcctcttc taaaccagct ggtttacaaa
     1561 gtggcaagag agatctccag tcctgatgat ggttttgaaa gtaaatcact ttatgaaagc
     1621 tggctggaaa aagacccttc acctgaaaat aaagagtgtc ctagaatcaa taagcttgga
     1681 tctgggagtg attttgaggc ttacttccaa agacttggaa ttgcttcagg cagagctcgc
     1741 tacactaaga acaagaaaac agataaatac agtagctatc ctgtatatca taccatctat
     1801 gagacgtttg aattagtaca gaacttttat gaccccacat ttaaaaagca actgtctgtg
     1861 gctcagttac gaggagcatt ggtatatgag cttgcagact ctgtggtcat tcctttcaac
     1921 attcaagact atgcaaaagc cttgaaaaac tatgcagcaa gtattttcaa catatccaag
     1981 aaacacgacc agcaattgag aaaccatgca gtgtcatttg atcccttatt ttctgctgtg
     2041 aaaaactttt cggaggctgc ttcagatttt cacagaagac ttacacaagt tgatcttaat
     2101 aatcctattg cagtgagaat tatgaatgac cagcagatgc ttcttgaaag agcattcata
     2161 gatcctcttg gtttacctgg gaggaagttc tacaggcaca tcatctttgc tccaagcagc
     2221 cacaacaaat atgccggaga atcattccct gggatttatg atgccatgtt tgatattgaa
     2281 aataaggcag acccttcctt ggcctgggca gaagtgaaga aacatatttc tattgcagct
     2341 tttacaattc aggcagcagc ggggacactg accaatgtgt tatagaaagg cttctgtcga
     2401 gtagcttcat caatggaaaa atattgcgtg gtgtctgaga gggaaactga tggggagtgg
     2461 gtcttgacca aatggattcc ctgctaacag tgctgctggg aatctgaaga aaaaacggtg
     2521 tcttttcctt tgatgtttta tgaaacctga caatcctaaa tctgtgattg tcatgacttg
     2581 ttttgtcctt ctaccttatt tatctgtaaa gagggctttt taactcttat atgcatatcc
     2641 agtggaagtg acagtgaaag aggaagcaac cccagtgctc acaggcagat gactggattc
     2701 actgtagtat atacacatag catgcatgca ttcttatatc aagtccgctt ggttctttac
     2761 ttcaccaaat aattgaaaat aaaaagttat ttaattttct ctgttagcat caagaaacat
     2821 caaaggagag ggtatgaaca gaatataaga gccagagaat gtgaaatggt aacctggaca
     2881 ttatcgttgc acacatgaac tgtacaactg tgttatctac gcataaccaa gcaaaagatt
     2941 ccagaagaga caggacattg actcatgagt cccacttgca aatgaacaac tactggtagt
     3001 taatagctag gggagaggaa gctagatttt aaggtctata gccactggta agttgtccag
     3061 tgttcagtaa acagatgcac taccacgaac atgcaagcaa atctaattaa atttaatgta
     3121 taaaaaaaaa gccatggaaa tattagagtg ttgggaagat aggttttgaa gggaatggga
     3181 gagtggtaag agatgggact taggggtgaa tattctcaaa gtatattatg caaatgcagg
     3241 aaattattaa agaataaaaa attaacctta gaagagcaca ctaactaaaa tagtaagcaa
     3301 cagctaagct tgagaacaag aagaaaggat cgcactcctc ttccatcttg gctaacaaga
     3361 cagtgattta atgagtgagg agtgttagac aaacacaaag cttgctgaac ccccttacga
     3421 tgtacatgag acaggtcaga aagacaagga caaggccact tagaaaagaa agggagtcct
     3481 tcaggagatc cattaggaag gttgagaacc actggtttag agtgtgagat cctatgcctt
     3541 atagttaata agccataggc tgcaatgttc ctagtggcaa aggagggggt gggggaaagg
     3601 tttcaatttt ttatagttaa tgaagccatt caaagatttg tcaccagtga cctcaaatga
     3661 ttaggttgtt tgataaactt ctttggggaa gtgtcagtca gctccttata gcagtagaag
     3721 cagagtcagc tctgaaggtg ggacgatatc agcagaagag catggccaag caccattatg
     3781 tttggaatct gatacctttc tactaatagt tatccttaaa tctgtgttaa gatctcacct
     3841 taataaatca catatttgcc tcatactg
//
