LOCUS       NM_026366               1791 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Mus musculus N-6 adenine-specific DNA methyltransferase 1
            (putative) (N6amt1), transcript variant 1, mRNA.
ACCESSION   NM_026366 XM_128330 XM_358879
VERSION     NM_026366.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Zhou,H.M., Xu,H.J., Sun,R.H., Zhang,M., Li,X.T., Zhao,Y.X.,
            Yang,K., Wei,R., Liu,Q., Li,S., Xue,Z., Hao,L.Y., Yang,L.,
            Wang,Q.H., Wang,H.J., Gao,F., Cao,J.L. and Pan,Z.
  TITLE     DNA N6-methyladenine methylase N6AMT1 controls neuropathic pain
            through epigenetically modifying Kcnj16 in dorsal horn neurons
  JOURNAL   Pain 165 (1), 75-91 (2024)
   PUBMED   37624905
  REMARK    GeneRIF: DNA N6-methyladenine methylase N6AMT1 controls neuropathic
            pain through epigenetically modifying Kcnj16 in dorsal horn
            neurons.
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Kusevic,D., Kudithipudi,S. and Jeltsch,A.
  TITLE     Substrate Specificity of the HEMK2 Protein Glutamine
            Methyltransferase and Identification of Novel Substrates
  JOURNAL   J Biol Chem 291 (12), 6124-6133 (2016)
   PUBMED   26797129
  REMARK    GeneRIF: HEMK2 requires a GQX3R motif for methylation activity.
REFERENCE   3  (bases 1 to 1791)
  AUTHORS   Liu,P., Nie,S., Li,B., Yang,Z.Q., Xu,Z.M., Fei,J., Lin,C., Zeng,R.
            and Xu,G.L.
  TITLE     Deficiency in a glutamine-specific methyltransferase for release
            factor causes mouse embryonic lethality
  JOURNAL   Mol Cell Biol 30 (17), 4245-4253 (2010)
   PUBMED   20606008
  REMARK    GeneRIF: Disruption of N6amt1 gene led to early embryonic
            lethality.
REFERENCE   4  (bases 1 to 1791)
  AUTHORS   Nie,D.S., Liu,Y.B. and Lu,G.X.
  TITLE     Cloning and primarily function study of two novel putative
            N5-glutamine methyltransferase (Hemk) splice variants from mouse
            stem cells
  JOURNAL   Mol Biol Rep 36 (8), 2221-2228 (2009)
   PUBMED   19116772
REFERENCE   5  (bases 1 to 1791)
  AUTHORS   Liu,Y., Nie,D., Huang,Y. and Lu,G.
  TITLE     RNAi-mediated knock-down of gene mN6A1 reduces cell proliferation
            and decreases protein translation
  JOURNAL   Mol Biol Rep 36 (4), 767-774 (2009)
   PUBMED   18389380
  REMARK    GeneRIF: suggested that mN6A1 participates in protein translation
            through interaction with eRF1
REFERENCE   6  (bases 1 to 1791)
  AUTHORS   Figaro,S., Scrima,N., Buckingham,R.H. and Heurgue-Hamard,V.
  TITLE     HemK2 protein, encoded on human chromosome 21, methylates
            translation termination factor eRF1
  JOURNAL   FEBS Lett 582 (16), 2352-2356 (2008)
   PUBMED   18539146
REFERENCE   7  (bases 1 to 1791)
  AUTHORS   Ratel,D., Ravanat,J.L., Charles,M.P., Platet,N., Breuillaud,L.,
            Lunardi,J., Berger,F. and Wion,D.
  TITLE     Undetectable levels of N6-methyl adenine in mouse DNA: Cloning and
            analysis of PRED28, a gene coding for a putative mammalian DNA
            adenine methyltransferase
  JOURNAL   FEBS Lett 580 (13), 3179-3184 (2006)
   PUBMED   16684535
  REMARK    GeneRIF: The murine PRED28 encodes two alternatively spliced RNA.
REFERENCE   8  (bases 1 to 1791)
  AUTHORS   Gitton,Y., Dahmane,N., Baik,S., Ruiz i Altaba,A., Neidhardt,L.,
            Scholze,M., Herrmann,B.G., Kahlem,P., Benkahla,A., Schrinner,S.,
            Yildirimman,R., Herwig,R., Lehrach,H. and Yaspo,M.L.
  CONSRTM   HSA21 expression map initiative
  TITLE     A gene expression map of human chromosome 21 orthologues in the
            mouse
  JOURNAL   Nature 420 (6915), 586-590 (2002)
   PUBMED   12466855
REFERENCE   9  (bases 1 to 1791)
  AUTHORS   Reymond,A., Marigo,V., Yaylaoglu,M.B., Leoni,A., Ucla,C.,
            Scamuffa,N., Caccioppoli,C., Dermitzakis,E.T., Lyle,R., Banfi,S.,
            Eichele,G., Antonarakis,S.E. and Ballabio,A.
  TITLE     Human chromosome 21 gene expression atlas in the mouse
  JOURNAL   Nature 420 (6915), 582-586 (2002)
   PUBMED   12466854
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY456393.1 and AC140319.3.
            
            On Apr 14, 2009 this sequence version replaced NM_026366.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY456393.1, AK145617.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-843               AY456393.1         1-843
            844-1630            AY456393.1         845-1631
            1631-1791           AC140319.3         72899-73059         c
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 49.52 cM"
     gene            1..1791
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /note="N-6 adenine-specific DNA methyltransferase 1
                     (putative)"
                     /db_xref="GeneID:67768"
                     /db_xref="MGI:MGI:1915018"
     exon            1..202
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..713
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /EC_number="2.1.1.72"
                     /note="isoform 1 is encoded by transcript variant 1;
                     N6-DNA methyltransferase A; M-N6DNA MTase A; HemK
                     methyltransferase family member 2; methylarsonite
                     methyltransferase N6AMT1; protein N(5)-glutamine
                     methyltransferase; N(6)-adenine-specific DNA
                     methyltransferase 1; lysine N-methyltransferase 9"
                     /codon_start=1
                     /product="methyltransferase N6AMT1 isoform 1"
                     /protein_id="NP_080642.1"
                     /db_xref="CCDS:CCDS28289.1"
                     /db_xref="GeneID:67768"
                     /db_xref="MGI:MGI:1915018"
                     /translation="MAAPSVPTPLYGHVGRGAFRDVYEPAEDTFLLLDALEAAAAELA
                     GVEICLEVGAGSGVVSAFLASMIGPRALYMCTDINPEAAACTLETARCNRVHVQPVIT
                     DLVHGLLPRLKGKVDLLVFNPPYVVTPPEEVGSRGIEAAWAGGRNGREVMDRFFPLAP
                     ELLSPRGLFYLVTVKENNPEEIFKTMKTRGLQGTTALCRQAGQEALSVLRFSKS"
     exon            203..289
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /inference="alignment:Splign:2.1.0"
     exon            290..380
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /inference="alignment:Splign:2.1.0"
     exon            381..464
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /inference="alignment:Splign:2.1.0"
     exon            465..606
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /inference="alignment:Splign:2.1.0"
     exon            607..1791
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1218..1223
                     /regulatory_class="polyA_signal_sequence"
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /note="hexamer: AACAAA"
     polyA_site      1233
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /note="major polyA site"
     polyA_site      1236
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
     regulatory      1615..1620
                     /regulatory_class="polyA_signal_sequence"
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /note="hexamer: AATAAA"
     polyA_site      1632
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
     regulatory      1638..1643
                     /regulatory_class="polyA_signal_sequence"
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /note="hexamer: AATAAA"
     regulatory      1648..1653
                     /regulatory_class="polyA_signal_sequence"
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
                     /note="hexamer: AATAAA"
     polyA_site      1659
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
     polyA_site      1668
                     /gene="N6amt1"
                     /gene_synonym="5830445C04Rik; Hemk2; Pred28"
ORIGIN      
        1 aagcgcatgc gcagcaagcc tgacgcgtgc gtactcaacg cccgcccata ggaagcggaa
       61 gggaaaagat ggcggcgccg agtgtcccca cgccgttgta cgggcacgtg ggtcgcggag
      121 ccttccgcga cgtgtacgag ccagcggagg acacgttcct gttactggac gcgctcgagg
      181 cggcggcggc cgagctagca ggagtggaaa tatgccttga agtaggagca ggatctggtg
      241 tggtgtctgc attcctggcc tccatgatag gtcctcgggc cttatacatg tgcactgata
      301 tcaaccctga ggcagccgca tgtaccttgg aaacagcacg ctgtaacaga gtccatgttc
      361 agccagtgat cacagatttg gtgcacggct tgctgcccag actgaagggg aaagtagacc
      421 tgctggtgtt taaccccccc tatgtagtga ctccgcctga agaggtagga agtcgtggaa
      481 tagaagcagc ctgggctggc ggcagaaacg gccgggaagt catggacagg ttcttcccac
      541 tggctccaga actcctctcc ccaagagggc tgttctactt agttaccgta aaagaaaaca
      601 atcccgagga aatctttaaa acaatgaaga caagaggtct gcaagggacc acagcacttt
      661 gcaggcaagc aggccaagaa gccctgtcag tcctcaggtt cagcaagtcc tagtgtcctg
      721 aaagccacag aagtaaacaa gagctgccgg cagctgaaca atcgctcagt aggtttcaga
      781 ctgatgccat tgcctggcca agaaatttta ttagaaatct gtcatttaaa aaaaaaaaaa
      841 aaagtggagc caggtggtgg tggcacacac ctctaattcc agcactctgg agacagaggc
      901 ggagaatccc tgaattcaag gccagccgaa atgcagagtg agttccagga cagtcagggc
      961 tgcacagaga aaccctgtct caaaaaccta aaaccaacca aaccaaagtg aaaagaaaag
     1021 gcaagaaacc cgtgagggct gaatggatag atggctcaga ggctgagaca ctgaccgcta
     1081 gtacaaagga ttggggctca gttctcagca cccacatggt agggtagttc acaagggatc
     1141 ctgccctatt ctgacctgta cctttggagg tcagaatgac atgcatacat actgcaggca
     1201 aaatatccat acacaaaaac aaaataaacc taacaagaag aaaagaaaaa gatagaaagc
     1261 tgggtcattt tgttttcaga gaatcaacat atgagttttt ctgtgcaagt gcaagtaaat
     1321 atgtattata tgtataccat attttatata tttatatatg aaggcacgtt tgatttagcc
     1381 ttagctataa tatagcaaag caatttaatt accctaaatg tccaccagaa aggacactgg
     1441 ttaaaatctc agcctagggc tggagagatg gctcaatggt taagagcact gactgctctt
     1501 ccagaggtcc tgagttcaat tcccagcaac cacatggtgg ctcacagcta tctgtaatga
     1561 gagccaatgc cctcttttgg tgtgtctgaa gacagctaca gtgtgctcat atacaataaa
     1621 taaaccttta aagaataaat aaagtaaaat aaaatctcat tgaatccatt ccactgaaca
     1681 tgtagccatg aaacaaggca ctgtgtagat cctgcacccg aggctttttg gtgaccagga
     1741 caactgagtg ctaagaggct ggagctgaat ttcatcccat acacgctatt t
//
