LOCUS       NM_144557               4762 bp    mRNA    linear   ROD 18-JUN-2024
DEFINITION  Mus musculus myosin VIIA and Rab interacting protein (Myrip), mRNA.
ACCESSION   NM_144557 XM_916811 XM_924502
VERSION     NM_144557.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Zhao,K., Matsunaga,K., Mizuno,K., Wang,H., Okunishi,K. and Izumi,T.
  TITLE     Functional hierarchy among different Rab27 effectors involved in
            secretory granule exocytosis
  JOURNAL   Elife 12, e82821 (2023)
   PUBMED   36803984
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4762)
  AUTHORS   Fan,F., Matsunaga,K., Wang,H., Ishizaki,R., Kobayashi,E.,
            Kiyonari,H., Mukumoto,Y., Okunishi,K. and Izumi,T.
  TITLE     Exophilin-8 assembles secretory granules for exocytosis in the
            actin cortex via interaction with RIM-BP2 and myosin-VIIa
  JOURNAL   Elife 6, e26174 (2017)
   PUBMED   28673385
  REMARK    GeneRIF: Here, the authors show that exophilin-8 accumulates
            granules in the cortical F-actin network not by direct interaction
            with myosin-Va, but by indirect interaction with a specific form of
            myosin-VIIa through its previously unknown binding partner,
            RIM-BP2.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4762)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   5  (bases 1 to 4762)
  AUTHORS   Brozzi,F., Lajus,S., Diraison,F., Rajatileka,S., Hayward,K.,
            Regazzi,R., Molnar,E. and Varadi,A.
  TITLE     MyRIP interaction with MyoVa on secretory granules is controlled by
            the cAMP-PKA pathway
  JOURNAL   Mol Biol Cell 23 (22), 4444-4455 (2012)
   PUBMED   22993210
REFERENCE   6  (bases 1 to 4762)
  AUTHORS   Kuroda,T.S. and Fukuda,M.
  TITLE     Functional analysis of Slac2-c/MyRIP as a linker protein between
            melanosomes and myosin VIIa
  JOURNAL   J Biol Chem 280 (30), 28015-28022 (2005)
   PUBMED   15927964
  REMARK    GeneRIF: Slac2-c acts as a functional myosin VIIa receptor and the
            Rab27A.Slac2-c x myosin VIIa tripartite protein complex regulates
            the transport of retinal melanosomes in pigment epithelium cells
REFERENCE   7  (bases 1 to 4762)
  AUTHORS   Fukuda,M.
  TITLE     Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through
            interaction with the GDP-bound form of Rab27A in PC12 cells
  JOURNAL   J Biol Chem 278 (17), 15390-15396 (2003)
   PUBMED   12590134
REFERENCE   8  (bases 1 to 4762)
  AUTHORS   Fukuda,M. and Kuroda,T.S.
  TITLE     Slac2-c (synaptotagmin-like protein homologue lacking C2
            domains-c), a novel linker protein that interacts with Rab27,
            myosin Va/VIIa, and actin
  JOURNAL   J Biol Chem 277 (45), 43096-43103 (2002)
   PUBMED   12221080
  REMARK    GeneRIF: Synaptotagmin-like protein homologue lacking C2 domains-c)
            is a novel linker protein that interacts with Rab27, myosin
            Va/VIIa, and actin
            GeneRIF: Slac2-c/MyRIP contains an N-terminal Slp homology domain
            (SHD) and functions as a linker protein that interacts with myosin
            Rab27A/B, VIIa/Va, and actin.
REFERENCE   9  (bases 1 to 4762)
  AUTHORS   El-Amraoui,A., Schonn,J.S., Kussel-Andermann,P., Blanchard,S.,
            Desnos,C., Henry,J.P., Wolfrum,U., Darchen,F. and Petit,C.
  TITLE     MyRIP, a novel Rab effector, enables myosin VIIa recruitment to
            retinal melanosomes
  JOURNAL   EMBO Rep 3 (5), 463-470 (2002)
   PUBMED   11964381
REFERENCE   10 (bases 1 to 4762)
  AUTHORS   Nagashima,K., Torii,S., Yi,Z., Igarashi,M., Okamoto,K., Takeuchi,T.
            and Izumi,T.
  TITLE     Melanophilin directly links Rab27a and myosin Va through its
            distinct coiled-coil regions
  JOURNAL   FEBS Lett 517 (1-3), 233-238 (2002)
   PUBMED   12062444
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC167243.3 and
            AC123708.17.
            
            On Oct 19, 2007 this sequence version replaced NM_144557.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK083225.1, AK148358.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-128               AC167243.3         153353-153480       c
            129-271             AC167243.3         121813-121955       c
            272-493             AC167243.3         69220-69441         c
            494-630             AC167243.3         40277-40413         c
            631-711             AC167243.3         37822-37902         c
            712-809             AC167243.3         35276-35373         c
            810-890             AC167243.3         35012-35092         c
            891-1037            AC167243.3         32815-32961         c
            1038-1191           AC167243.3         29892-30045         c
            1192-1817           AC167243.3         24575-25200         c
            1818-2057           AC167243.3         15972-16211         c
            2058-2252           AC167243.3         3832-4026           c
            2253-2414           AC123708.17        161096-161257       c
            2415-2519           AC123708.17        157867-157971       c
            2520-2580           AC123708.17        157704-157764       c
            2581-2699           AC123708.17        155101-155219       c
            2700-4762           AC123708.17        147831-149893       c
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 71.5 cM"
     gene            1..4762
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /note="myosin VIIA and Rab interacting protein"
                     /db_xref="GeneID:245049"
                     /db_xref="MGI:MGI:2384407"
     exon            1..128
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            129..271
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     CDS             162..2732
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /note="exophilin 8; slp homolog lacking C2 domains c;
                     myosin-VIIa- and Rab-interacting protein;
                     synaptotagmin-like protein lacking C2 domains C"
                     /codon_start=1
                     /product="rab effector MyRIP"
                     /protein_id="NP_653140.1"
                     /db_xref="CCDS:CCDS23627.1"
                     /db_xref="GeneID:245049"
                     /db_xref="MGI:MGI:2384407"
                     /translation="MGRKLDLSGLTDDETEHVLQVVQRDFNLRKKEEDRLSEMKQRLA
                     EENSKCSILSKHQKFVERCCMRCCSPFTFLVNARRRCGECKFSVCKSCCSYQKHEKLW
                     VCCVCQQARLLRTQSLEWFYNNVKSRFKRFGSAKVLKNLYRKHRLESGACFDILGGGL
                     FEPNLENEGSISGSDSTFYRQSEGHSMMDTLAVALRVAEEAIEEAISKAESHGDSLDK
                     QNEASYLRDHKQELTEELAGTILQRIIRKQKDKAELRAEEEEPEWPRSQSGSVKARGE
                     GTTAPPGRHKARATFRRSQSAFSFTMEDALKSGSAEAAPRSPKDRAQRLLEEAALPSW
                     RSMDGLDGTNLAPLLQSPDGNWMTLKDGSRQPPTRLLTKPKSGTFQALEVASSVTSAY
                     DEIGSDSEEDFDYSEALSKLCPPSQSRLKQPQPQPTQAQSSGQGPLATSPSNPEAMCS
                     DSETSSTSSSREAGCRAKLSWLQRKAPKNPAVEKMPLQGELDVNFNPQAAGGETSDSS
                     DPEETLRTAERRARRWRRARVGPEESNRGLPSPGAHPRALHTAQVSDNVSETDISNET
                     QNSRSSTDSVEEKLRNRLYELAMKMSEKETSSGEDQESESKAEPKNQKGSLSSEENNQ
                     GVQEELKKKCSAVSLCNISTEVLKVINATEELIAESAGPWEIPPVSTDRENGMFPLGT
                     DQVRLDKQLTSLEENVYLAAGTVYGLEGQLSELEDAARCIHSSTGETELADLEDQVAA
                     AAAQVHHAELQISDIESRISALTIAGLNIAPCVRLTRRRDQKQRSQVQTIDTSRQQRR
                     KLPAPPVKAEKIEASSVTPIKTFNRNFLLQGSSTNRPTASTGDTKDLMEPDLESAVMY
                     "
     exon            272..493
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            494..630
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            631..711
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            712..809
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            810..890
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1037
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            1038..1191
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            1192..1817
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            1818..2057
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            2058..2252
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            2253..2414
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            2415..2519
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            2520..2580
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            2581..2699
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
     exon            2700..4762
                     /gene="Myrip"
                     /gene_synonym="A230081N12Rik; Slac2-c; Slac2c"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agcctcggga tcgcgcgctg gacgcaggct gcggcaggtg acccggggcc atccgaggcg
       61 cggtggctgt gagcctggcc gccatccatg agctccgccg cggacccact ccaggccggc
      121 caccccggat ctagttcagt tgtccgtgtt taactctgat catggggagg aagctggacc
      181 tgtcgggtct gaccgacgat gagaccgagc atgttctcca agtggtgcag agagacttca
      241 atcttcgcaa aaaggaggag gaccggctaa gtgagatgaa gcagaggctg gccgaggaga
      301 acagcaagtg cagcatcctc tcgaagcacc agaagttcgt ggagcgctgc tgcatgcggt
      361 gctgctcgcc cttcaccttc ctggtcaacg ccaggcgccg ctgcggggaa tgcaagttca
      421 gcgtctgcaa gagctgctgc tcctaccaga agcacgagaa gctatgggtt tgctgtgtct
      481 gccagcaagc caggcttctg aggacacagt ctctggagtg gttctacaac aatgtgaaaa
      541 gccgcttcaa gcgctttggc agtgccaagg tcctgaagaa cctgtacaga aaacaccgtc
      601 tggagagcgg tgcctgcttc gacatcctag gaggaggatt gtttgaacca aacctggaga
      661 atgaagggag catttcagga agtgactcca cgttctacag gcagtcagaa gggcacagca
      721 tgatggacac cttggctgtg gccctacgag tggcagaaga ggccatcgag gaggccattt
      781 ccaaagcaga atcccatggg gacagcctgg acaaacagaa cgaggccagc tacctgcggg
      841 accacaagca agagctaact gaggagctgg ccggcaccat cctgcagagg attataagaa
      901 agcagaagga taaagctgag ctgcgagctg aggaggagga gccagagtgg ccacggtccc
      961 agagtggcag tgtgaaggcc aggggtgagg ggaccacggc ccctccagga cgacacaaag
     1021 cacgtgccac cttcaggcgg tcccagtctg cattctcctt cactatggaa gatgctctga
     1081 agtctggttc agcagaggct gcaccaaggt cacccaagga ccgcgcccag cgtctgctgg
     1141 aggaggccgc tctgcccagc tggaggagca tggatggact ggacggaaca aacctggccc
     1201 cacttttgca gagccccgat gggaactgga tgaccttgaa ggacggtagt agacagcccc
     1261 caacccgcct gctgaccaaa ccgaagagtg ggacctttca ggccctggag gtggcgtcca
     1321 gtgtgacgtc tgcctatgat gagatcggtt ccgacagtga ggaagacttt gattacagcg
     1381 aagccttgag taagctgtgc ccaccgtccc agagcaggct caagcaaccc cagcctcagc
     1441 ctacacaggc ccagagttct ggccaaggtc ccttagccac ctctccctca aaccctgagg
     1501 ccatgtgctc ggattcagag acatcttcca ccagctcatc tcgggaagct ggctgccggg
     1561 ctaagctgtc ctggctgcag aggaaggccc ccaagaaccc cgcagtggag aagatgcccc
     1621 tccagggaga actggacgtc aatttcaacc ctcaggcagc cggtggagag acttccgaca
     1681 gtagcgatcc agaggagacc ctccgcactg cagagcgccg ggccaggagg tggagaaggg
     1741 cacgagtggg ccccgaggag tcaaacaggg gtctgccttc acctggtgcc catccacggg
     1801 cactgcacac ggctcaggtg tcagacaacg tgtcagagac tgacatcagc aacgagactc
     1861 agaattcccg gtccagcacg gactcagtag aggagaagct gagaaataga ctgtatgagt
     1921 tagccatgaa aatgagcgaa aaggagactt cttctgggga ggaccaggag tccgagtcca
     1981 aggcggaacc taagaaccag aagggaagtc tctcttctga ggagaacaac cagggtgtcc
     2041 aggaggagct gaagaagaag tgttctgctg tttctctctg caacatctcc acagaagtcc
     2101 tgaaggtcat caatgctacc gaggagttga tcgcagagtc tgcagggccc tgggagatcc
     2161 ccccagtctc cactgacaga gaaaacggga tgtttcctct tgggacagac caagtgagac
     2221 tggacaagca gctgacgtcc ttggaagaaa acgtgtacct ggcagcgggc accgtgtatg
     2281 ggctggaggg ccagctgagt gagctggaag acgctgcccg ctgcatccac agcagcactg
     2341 gcgagacgga gctggcagac ctggaggacc aggtggctgc agctgcagcc caggtgcacc
     2401 acgccgaact ccagatctcg gacatcgaga gccgcatttc agccctgacc atcgctggct
     2461 taaacatagc accgtgtgtg cgtctcacac gaagacggga ccagaagcaa aggagccagg
     2521 tacaaactat agacacgtcg aggcagcaga ggaggaaact gcctgcccct cctgtgaaag
     2581 ctgaaaaaat cgaagcatct tccgtgaccc ccattaagac attcaaccgc aacttccttc
     2641 tccaaggctc ctccacaaac aggcccacag caagtaccgg tgacaccaag gacttgatgg
     2701 agcctgatct ggagtcagct gtgatgtact aaagcaggac tccacacaca gccagtgacc
     2761 cactgcctcc ggccgtacac gacagtgcct tgacccaaca gccatcgagt actgtgtgga
     2821 tttccactga ggcgagggcc tgggagggct gggtgagggg gctgcagtgc actgctgcac
     2881 cgggctgtct tgatacctca cccagaaggc cgtctcagcc cgcaacactg aggtctcgcc
     2941 cactctctgc cttaggctcc caagggaatt tgagaccgaa tatccagatg ctggcttcca
     3001 atagcagagc tctgtgttca gaagatgcat tgtgcctgtt taattttgct cctctgtgct
     3061 gtctttacca gccccgtgtg tttttaatcc ctttaatccc catcaacagt tcatcctaca
     3121 gaaaactgct ggagaggagc tgggggctag gaaggtgggg atcactacca tcacttagtc
     3181 gtgcttatca cctcctaacc gatgctaaga gcagggggca ctagaaacag tctcatttgg
     3241 aaaatgcttg gccacctcca gctaagcccc cagccccgtg cctctgtcct ccccgttgct
     3301 tggccgccac taaaggaaaa gctctgatcc accgatgcca tacccgggtc tctctgaaac
     3361 catgtcttat tctcaatttg gggttcttgc atggatttac tcagggggag aggggcctaa
     3421 gggacgatgt cactattaat ctaagataaa ggatactgtt cagagtcaag gtgatatatg
     3481 gcttggtcac gaatgagcta ttgtaaaata gaactacaca agaaaagtgt tctatcctct
     3541 aaaagatatc tcctgccaaa ttcaacaaga ttccgaatga cagccatgat aaaggtggtc
     3601 agcaacccca cccccttaag agaaaatcag tgtgaacggt gaggggcgca gagctgaccc
     3661 tcaccccaag ttcactgtgt ttcagggcag gggctgaaat aaatgcatga gtatctaaag
     3721 tgtgtccatg cacacaataa gactgacccc atgctggtct cgctgggtgc ctgcagcatg
     3781 tttttaacgt tggtttctgg gattttctga atcatagggt gtgatgggga gagggtagag
     3841 gagggaggcg cgggggaggg ggtggccatt aggtgctggt tctggagggc acagtgtgtc
     3901 cagttttgtc tagtctcttc tctcttccgg ttttccagaa gtcccataac taaaggtcag
     3961 accagtgaac ttgctgaagc caggcgtgtg agctggttct aactggagtg ccccccaccc
     4021 ccagaagcca gccctgtctt cacttccaag ttctctggtt ctcctatgca tcctcagatt
     4081 ggattgaacc gccctccctg tgtcctccct gtgtcctccc tgtgtcctcc ctccttgttt
     4141 gccattgctg ctgatggggg tccttgtgtc ttcctatgat gctcctgctg atgtttgtgt
     4201 caggactcca tggaaaaagt aaacctttgg tgttttatcc agtgggtgtg gttcacacta
     4261 aaacaaagtt taaaaaacac aacaaaaaga tctgactttc aaacaggacg tttttttctt
     4321 taagaggcac gaggctgcct ttgtgctacc agtgttaaaa tactcagttg tttttttttg
     4381 acaaagcgtg tactgtgtaa gccttgcaaa aaataataat aagagcaaca acaacaacaa
     4441 cgaagaaaaa gcctgttcta tggcgttcga acttttgtaa aaggtttcct tgtgccaaat
     4501 aagtacaaag atttaattta ctatttaaac cataaacata ctatagttcc agaaattatt
     4561 ttgtcatcag tgattttgat ctttagtgtc aatatttata tttagattaa tttttataaa
     4621 tgaaatattt tgatggtcta agaaaatgag gacaggaagg atgcgatctc tgacggcttc
     4681 tcgatgcctt catgtctcac acactcggcc aagaatcact gtcaagagaa acgaccacgg
     4741 ccaagcagta aagtcacctg tg
//
