LOCUS       NM_001033481            5580 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus myelin regulatory factor (Myrf), transcript variant 1,
            mRNA.
ACCESSION   NM_001033481 XM_359374 XM_888586 XM_981099
VERSION     NM_001033481.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Xu,X., Fang,M., Chen,L., Huang,H., Dai,Z.M., Yang,J. and Qiu,M.
  TITLE     Nzf2 promotes oligodendrocyte differentiation and regeneration via
            repressing HDAC1-mediated histone deacetylation
  JOURNAL   Sci Adv 10 (50), eadf8405 (2024)
   PUBMED   39671488
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Duncan,G.J., Ingram,S.D., Emberley,K., Hill,J., Cordano,C.,
            Abdelhak,A., McCane,M., Jenks,J.E., Jabassini,N., Ananth,K.,
            Ferrara,S.J., Stedelin,B., Sivyer,B., Aicher,S.A., Scanlan,T.S.,
            Watkins,T.A., Mishra,A., Nelson,J.W., Green,A.J. and Emery,B.
  TITLE     Remyelination protects neurons from DLK-mediated neurodegeneration
  JOURNAL   Nat Commun 15 (1), 9148 (2024)
   PUBMED   39443516
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5580)
  AUTHORS   Yalcin,B., Pomrenze,M.B., Malacon,K., Drexler,R., Rogers,A.E.,
            Shamardani,K., Chau,I.J., Taylor,K.R., Ni,L.,
            Contreras-Esquivel,D., Malenka,R.C. and Monje,M.
  TITLE     Myelin plasticity in the ventral tegmental area is required for
            opioid reward
  JOURNAL   Nature 630 (8017), 677-685 (2024)
   PUBMED   38839962
REFERENCE   4  (bases 1 to 5580)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   5  (bases 1 to 5580)
  AUTHORS   Ji,X.Y., Guo,Y.X., Wang,L.B., Wu,W.C., Wang,J.Q., He,J., Gao,R.,
            Rasouli,J., Gao,M.Y., Wang,Z.H., Xiao,D., Zhang,W.F., Ciric,B.,
            Zhang,Y. and Li,X.
  TITLE     Microglia-derived exosomes modulate myelin regeneration via
            miR-615-5p/MYRF axis
  JOURNAL   J Neuroinflammation 21 (1), 29 (2024)
   PUBMED   38246987
  REMARK    GeneRIF: Microglia-derived exosomes modulate myelin regeneration
            via miR-615-5p/MYRF axis.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5580)
  AUTHORS   Yu,Y., Chen,Y., Kim,B., Wang,H., Zhao,C., He,X., Liu,L., Liu,W.,
            Wu,L.M., Mao,M., Chan,J.R., Wu,J. and Lu,Q.R.
  TITLE     Olig2 targets chromatin remodelers to enhancers to initiate
            oligodendrocyte differentiation
  JOURNAL   Cell 152 (1-2), 248-261 (2013)
   PUBMED   23332759
REFERENCE   7  (bases 1 to 5580)
  AUTHORS   Koenning,M., Jackson,S., Hay,C.M., Faux,C., Kilpatrick,T.J.,
            Willingham,M. and Emery,B.
  TITLE     Myelin gene regulatory factor is required for maintenance of myelin
            and mature oligodendrocyte identity in the adult CNS
  JOURNAL   J Neurosci 32 (36), 12528-12542 (2012)
   PUBMED   22956843
  REMARK    GeneRIF: Expression of MRF within the adult central nervous system
            (CNS) is critical to maintain oligodendrocyte identity and
            integrity of CNS myelin.
REFERENCE   8  (bases 1 to 5580)
  AUTHORS   Weng,Q., Chen,Y., Wang,H., Xu,X., Yang,B., He,Q., Shou,W., Chen,Y.,
            Higashi,Y., van den Berghe,V., Seuntjens,E., Kernie,S.G.,
            Bukshpun,P., Sherr,E.H., Huylebroeck,D. and Lu,Q.R.
  TITLE     Dual-mode modulation of Smad signaling by Smad-interacting protein
            Sip1 is required for myelination in the central nervous system
  JOURNAL   Neuron 73 (4), 713-728 (2012)
   PUBMED   22365546
  REMARK    Erratum:[Neuron. 2012 Oct 18;76(2):462]
REFERENCE   9  (bases 1 to 5580)
  AUTHORS   Yan,X., Lukas,J., Witt,M., Wree,A., Hubner,R., Frech,M.,
            Kohling,R., Rolfs,A. and Luo,J.
  TITLE     Decreased expression of myelin gene regulatory factor in
            Niemann-Pick type C 1 mouse
  JOURNAL   Metab Brain Dis 26 (4), 299-306 (2011)
   PUBMED   21938520
  REMARK    GeneRIF: NPC1 protein was expressed in oligodendrocytes and the
            amount of myelin protein was generally decreased in all parts of
            the brain and spinal cord in NPC1-mutant mice
REFERENCE   10 (bases 1 to 5580)
  AUTHORS   Emery,B., Agalliu,D., Cahoy,J.D., Watkins,T.A., Dugas,J.C.,
            Mulinyawe,S.B., Ibrahim,A., Ligon,K.L., Rowitch,D.H. and
            Barres,B.A.
  TITLE     Myelin gene regulatory factor is a critical transcriptional
            regulator required for CNS myelination
  JOURNAL   Cell 138 (1), 172-185 (2009)
   PUBMED   19596243
  REMARK    GeneRIF: Study identifies gene model 98, which we have named myelin
            gene regulatory factor (MRF), as a transcriptional regulator
            required for CNS myelination.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC132148.3.
            
            On Apr 8, 2022 this sequence version replaced NM_001033481.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMN00849374, SAMN00849375
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-173               AC132148.3         95728-95900         c
            174-261             AC132148.3         84189-84276         c
            262-525             AC132148.3         83822-84085         c
            526-587             AC132148.3         80618-80679         c
            588-867             AC132148.3         79541-79820         c
            868-1118            AC132148.3         78486-78736         c
            1119-1242           AC132148.3         78286-78409         c
            1243-1438           AC132148.3         76347-76542         c
            1439-1515           AC132148.3         74964-75040         c
            1516-1626           AC132148.3         74691-74801         c
            1627-1717           AC132148.3         74207-74297         c
            1718-1918           AC132148.3         73633-73833         c
            1919-2030           AC132148.3         73317-73428         c
            2031-2140           AC132148.3         72589-72698         c
            2141-2301           AC132148.3         71787-71947         c
            2302-2374           AC132148.3         71589-71661         c
            2375-2463           AC132148.3         71230-71318         c
            2464-2532           AC132148.3         70911-70979         c
            2533-2621           AC132148.3         70186-70274         c
            2622-2789           AC132148.3         69929-70096         c
            2790-3041           AC132148.3         69228-69479         c
            3042-3129           AC132148.3         67611-67698         c
            3130-3204           AC132148.3         67315-67389         c
            3205-3310           AC132148.3         66877-66982         c
            3311-3385           AC132148.3         65768-65842         c
            3386-5580           AC132148.3         63440-65634         c
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 6.54 cM"
     gene            1..5580
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /note="myelin regulatory factor"
                     /db_xref="GeneID:225908"
                     /db_xref="MGI:MGI:2684944"
     exon            1..173
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..3466
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /note="isoform 1 is encoded by transcript variant 1;
                     myelin gene regulatory factor"
                     /codon_start=1
                     /product="myelin regulatory factor isoform 1"
                     /protein_id="NP_001028653.1"
                     /db_xref="CCDS:CCDS50387.1"
                     /db_xref="GeneID:225908"
                     /db_xref="MGI:MGI:2684944"
                     /translation="MEVVDETEALQRFFEGHDISGALEPSNIDTSILEEYIGKEDASD
                     LCFPEISAPASTASFPHGPPAIPGSSGLHHLSPPGSGPSPGRHGPLPPPTYGTPLNCN
                     NNNGMGTAPKPFLGGSGPPIKAEPKAPYAPGTLPDSPPDSGSEAYSPQQVNDPHLLRT
                     ITPETLCHVGVSSRLEHPPPPPAHLPGPPPPPPPPPHYPVLQRDLYMKAEPPVPPYAA
                     MGPGLVPPELHHTQQTQVLHQLLQQHGAELPPHPSKKRKHSESPPNTLNAQMLNGMIK
                     QEPGTVTALPPHPARAPSPPWPPQGPLSPGTGSLPLSIARAQTPPWHPPGAPSPGLLQ
                     DSDSLSGSYLDPNYQSIKWQPHQQNKWATLYDANYKELPMLTYRVDADKGFNFSVGDD
                     AFVCQKKNHFQVTVYIGMLGEPKYVKTPEGLKPLDCFYLKLHGVKLEALNQSINIEQS
                     QSDRSKRPFNPVTVNLPPEQVTKVTVGRLHFSETTANNMRKKGKPNPDQRYFMLVVAL
                     QAHAQNQNYTLAAQISERIIVRASNPGQFESDSDVLWQRAQLPDTVFHHGRVGINTDR
                     PDEALVVHGNVKVMGSLMHPSDLRAKEHVQEVDTTEQLKRISRMRLVHYRYKPEFAAS
                     AGIEATAPETGVIAQEVKEILPEAVKDTGDVVFANGKTIENFLVVNKERIFMENVGAV
                     KELCKLTDNLETRIDELERWSHKLAKLRRLDSLKSTGSSGAFSHAGSQFSRAGSVPHK
                     KRPPKLANKSSPAVPDQACISQRFLQGTIIALVVVMAFSVVSMSTLYVLSLRSEEDLV
                     DADGRSSQSFGTTQLRQSSMTTGLPGTQPSLLLVTKSASGPALRALDLCSSQPCPIVC
                     CSPPVSSPATDPALGPTLTPTPSPSSNPKHSGPGQMAPLPVTNIRAKSWGISANGISY
                     SKHSKSLEPLASPVVPFPGGQSKTKNSPSFNLQSRARRGAPQPSPSPAQFTQTQGQLD
                     PAPSLTSIQLLENSMPITSQYCVPEGACRLGNFTYHIPVSSSTPLHLSLTLQMNSSTP
                     VSVVLCSLTSEEEPCEEGGFLQRFHPHQDTQGTSHQWPVTILSFREFTYHFRVTLLGQ
                     ANCSSEAIVQPATDYYFHFYRLCD"
     exon            174..261
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            262..525
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            526..587
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            588..867
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            868..1118
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1242
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1438
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            1439..1515
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            1516..1626
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            1627..1717
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            1718..1918
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            1919..2030
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            2031..2140
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            2141..2301
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            2302..2374
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2463
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            2464..2532
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2621
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2789
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            2790..3041
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            3042..3129
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            3130..3204
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            3205..3310
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            3311..3385
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     exon            3386..5580
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5549..5554
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /note="hexamer: AATAAA"
     polyA_site      5580
                     /gene="Myrf"
                     /gene_synonym="6030439E18; Gm1804; Gm98; Mrf"
                     /note="major polyA site"
ORIGIN      
        1 agagcctggc cggactgtac cgggagccca gccgggactg tcgcgcgggc cgcgccggcg
       61 atgccgcgcc cccgggccag gctgtagcgg ggctgcggcc ggagcgcacg cggcgggcag
      121 gcgggccatg gaggtggtgg acgagaccga agcgctgcag cgcttcttcg aaggccatga
      181 catcagcggt gccctggagc cctccaatat agacaccagt atcctggagg agtacattgg
      241 caaagaggac gcctctgatc tctgcttccc tgagatctct gcaccagcca gcactgcctc
      301 cttcccccac gggccaccgg ccattcccgg ctccagcggg ctccaccatc tgagcccccc
      361 tgggagcgga ccatcccctg ggcgccatgg ccccctccca cccccgacct acggcacccc
      421 actcaactgc aacaacaaca acggcatggg caccgcccct aagcccttcc tggggggctc
      481 tgggcctccc atcaaagcag agccaaaggc cccctatgcc ccaggcacac tgccagactc
      541 gcccccagac tcaggctctg aggcctactc ccctcagcag gtgaatgacc cccatcttct
      601 acgcaccata accccggaga ctctatgcca cgtgggagtt tcttcccgcc tggagcaccc
      661 gcccccacct ccagcccacc tgccaggccc accaccaccg ccacctcccc cacctcacta
      721 ccctgtcctg caacgggacc tctacatgaa ggctgagccc cctgtacccc cttatgctgc
      781 catggggccg ggtctggtgc cccccgagct ccatcacacc cagcagaccc aggtgctaca
      841 ccagctgctg caacagcatg gagctgaact ccctccacac ccctctaaga agaggaagca
      901 ctctgaatca ccccccaaca ccctcaatgc ccaaatgctg aacggaatga tcaagcagga
      961 acctgggact gtcacagcct tgcctccaca ccctgccaga gccccatccc ctccatggcc
     1021 tcctcaaggc ccactgtcac ctggcactgg atccttgccc ctcagcattg cccgagccca
     1081 gactccaccc tggcacccgc caggtgcacc ctccccaggc ctcctgcagg acagtgacag
     1141 cctcagtggc tcctatttgg accccaacta ccaatccatc aaatggcagc cgcatcagca
     1201 gaacaagtgg gcgaccctgt atgacgctaa ctacaaggag ctgcctatgc tcacctatcg
     1261 tgtggacgct gacaagggct tcaacttttc cgtgggcgac gatgcttttg tgtgccagaa
     1321 gaagaaccac ttccaggtga ccgtgtacat cggcatgctg ggggagccca agtacgtcaa
     1381 gacaccggaa ggcctcaagc ccctggactg cttctatctg aagctgcatg gtgtgaagct
     1441 agaggccctg aaccagtcta tcaacattga gcagtcacag tcagacagaa gcaagaggcc
     1501 cttcaacccc gtcacggtca atcttccccc tgagcaggtc acaaaagtga ccgtggggcg
     1561 gctccatttc agtgagacca ctgccaacaa catgcgcaag aagggcaagc ccaaccctga
     1621 ccagaggtat ttcatgctgg tggtggccct ccaggcacat gcacagaacc agaactacac
     1681 actggcagcc cagatctcag agcgtatcat tgtgcgggcc tctaacccag gccagtttga
     1741 aagtgacagt gacgtgctgt ggcaacgggc gcagctgcca gatacggtct tccaccatgg
     1801 ccgtgtgggc atcaacaccg accggccaga tgaggcattg gtcgtccacg gcaacgtgaa
     1861 ggtcatgggt tctcttatgc acccttccga tctgcgggcc aaggagcacg tgcaggaggt
     1921 ggacaccacc gagcagctga agaggatctc tcggatgcgg ctggtgcact acagatacaa
     1981 gcctgagttc gctgctagcg caggcattga agccaccgca ccggagacag gtgtcatcgc
     2041 ccaggaagtg aaggagatcc tgcctgaagc tgtgaaggac acaggggatg tagtctttgc
     2101 caatgggaaa accatagaga acttccttgt agtgaacaag gagcgaatct tcatggagaa
     2161 tgtgggggct gtgaaggagt tatgcaaact gacagacaac ctagagactc gcattgatga
     2221 gttggagcga tggagccaca agctggccaa gctgcggcgc cttgacagcc tcaagtcaac
     2281 tggcagctca ggggctttca gccatgcagg gagccagttt agccgggcag gcagtgtccc
     2341 ccacaaaaag aggcccccta aactggccaa taagtcatcg ccagcggtcc cagaccaggc
     2401 ctgcatcagt cagcgttttc tgcagggaac tatcatagct ctggtggtgg tcatggcctt
     2461 cagcgtggtg tctatgtcca cactatatgt gctgagcctg cgctctgaag aggacctggt
     2521 ggatgctgat ggcaggtcca gccagagctt cgggaccaca cagctccgac agtcctccat
     2581 gaccaccgga ctaccaggca cacagccctc tttgctgctg gttaccaagt cagcctcggg
     2641 tccagctctc cgtgccttgg acctgtgctc cagccagccc tgccccatcg tctgctgctc
     2701 tcctcccgtc tccagtcctg ctacagatcc tgcccttggc cccactctta ctcctactcc
     2761 aagccccagc tctaacccca agcactcagg ccctggccag atggccccac tgccagtcac
     2821 caacatcaga gccaaatcct ggggcatctc agctaatggc atcagctatt ccaagcattc
     2881 caagagcctg gaacctctgg ccagtcctgt ggtccccttt cctggagggc agagcaagac
     2941 caagaacagc cccagcttca atctccaaag tcgagcccgc agaggagccc cgcaacccag
     3001 cccaagccct gcccaattca cacagaccca gggccagcta gacccagcgc catccctgac
     3061 ctccatccag ctgctggaga attccatgcc tatcacttct cagtactgtg tgccagaagg
     3121 tgcttgcagg cttggcaact tcacctacca catccctgtc agcagcagca caccactgca
     3181 cctcagcctg accctgcaga tgaattcctc cacccctgtg tccgtggtac tgtgcagcct
     3241 gacatcggag gaggagccct gtgaggaggg aggctttttg cagaggttcc acccgcatca
     3301 ggacacccag ggcacctctc atcagtggcc agtaaccatc ctgtccttcc gtgaattcac
     3361 ataccacttc cgggtgacat tgctgggtca ggccaactgc agctcagagg ccatcgttca
     3421 gccagccacc gactactact tccacttcta ccgcctgtgt gactgagctg cctcctgagg
     3481 cagtgccaca gccgggactg gagtccctgg gccctcaaca ctggatgcaa atgtgttaca
     3541 ctggagcctg ctgcaggcca gctctctgct cttcactgct tcccttgact ggggagactg
     3601 gaagtcctta cactggagtt gctgggccaa ctggtcaccc tcacacagca cagagggggc
     3661 tagagggaac agcctcctgg gtcttccttc tggtcatctc cttggggcag cacaggacca
     3721 cttcatggct gtctagttat ggtccctgga gggctatttg cccaccttca ctctccctgc
     3781 cttcccttct gagttgtggt agagcaaaga cccacagggg ttggacccag acctcttaaa
     3841 acctggctgg acccccatga gtccacgcag agtcaataac tcacctgtca tgctgttccc
     3901 aggagggaca gcttggtagg aactttctgg ggtttgactg ttacactaga catggatttc
     3961 taagctttaa gtgtggttgg tttaggagag ttttccttgg gagagctagc cctgaggtag
     4021 tcagggctag ctccggatgg ctggagaagc tgaccttgtg ccttaagtct actagtgcct
     4081 gatggagcca ccctcatagg gctgaaccag tggggctgac gggccaggtc agccttggac
     4141 ccttgatcct ggaggaccct ggaaggggtg gttccctgta agctcccaag atctatgttc
     4201 ctgggagttt cccaaactca tgatggagtt ggtgacaaga gcagaggagc aagaatccct
     4261 gcaagcagta gactggtttc cactggaggg tgaatgcaga taccttttat taacgatata
     4321 cttactaaat gcacttaaac caggtaccaa ggagtaggac agaactggaa agctgagtgg
     4381 gcctcagtgt ggagaggtac tagtggacag gactttcatg gcttgggtcc atgctcctcc
     4441 cacacccaag aggagtggtg ggtttgagtt tggaggtttt actcaagtga cagcttcagc
     4501 atttcttggt agcacctaca gcattctgat tacatgcagg ttcctggaag gagtgtgtca
     4561 atcatttagc tgcatgtcat cggctcagca gtgggaagga aagggtactg ctctgacctt
     4621 gtgtattctg actctttgga aaccaggccc tgcttttgcc aagcctccca tcctgagctc
     4681 taaatggaag gcctttctat ggggtagcta agtttgggaa gcctatgaac tgtgggtcct
     4741 gccaaggttc tcaaagcact tatcctcagg atagggaaat agcagggacg ccttgtcccc
     4801 aagacttctc cccaacttgg ctccattcct gcattctgcc ataggaatca caacttccca
     4861 catcaggcca tgcttgccta agacctcgtt ctgttctccc ataaggccgt ccttcccaca
     4921 gtaccttaag attgcccagc ctccctgctc tagtggctgg ggctccctta ccagaggggc
     4981 aagtctggcc cctcagtcct taactggaaa gtgaccctct tctacccctg gagtccatct
     5041 ggacagagcc agcctgaggc ccacgggcag ctgcctgttt gcatgcctgg gaagaggtcc
     5101 tcagtgtgag ttggggtcaa acttgggctc caagctgctt gagggggtca accttggacc
     5161 aaagttgcct taagccccaa gtataaaaga gcttagggga aggtgtaagg gccacaggct
     5221 ggaagctgac agcctagcag gcactccctt cctctcttcc ttctgctgtg ttcctatagc
     5281 cactgggcta atctccccct tgctttaggc tgtatatagc cccccccaac cccccagcac
     5341 agatccctgc ccatgctgtt ccccttagca gcctgcctgt tcatagagcg tttcccctct
     5401 tgctaactcc ccattttctc cgtgagctct gactcctatt tatcatatac tcactctgac
     5461 tctgaggaag gcagagaggt gccacttggc ctacttgctt cctgtcccca agacagtcct
     5521 tttcttctga agtaaatata catatataaa taaatgtata aatactgctt tgtatctgca
//
