LOCUS       NM_145136               8086 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus myocardin (Myocd), transcript variant 1, mRNA.
ACCESSION   NM_145136
VERSION     NM_145136.5
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8086)
  AUTHORS   Huycke,T.R., Hakkinen,T.J., Miyazaki,H., Srivastava,V., Barruet,E.,
            McGinnis,C.S., Kalantari,A., Cornwall-Scoones,J., Vaka,D., Zhu,Q.,
            Jo,H., Oria,R., Weaver,V.M., DeGrado,W.F., Thomson,M.,
            Garikipati,K., Boffelli,D., Klein,O.D. and Gartner,Z.J.
  TITLE     Patterning and folding of intestinal villi by active mesenchymal
            dewetting
  JOURNAL   Cell 187 (12), 3072-3089 (2024)
   PUBMED   38781967
REFERENCE   2  (bases 1 to 8086)
  AUTHORS   Yuan,W., Lin,H., Sun,Y., Liu,L., Yan,M., Song,Y., Zhang,X., Lu,X.,
            Xu,Y., He,Q., Ouyang,K., Zhang,C., Pan,Y., Huang,Y., Li,Y., Lu,X.
            and Liu,J.
  TITLE     Myocardin reverses insulin resistance and ameliorates
            cardiomyopathy by increasing IRS-1 expression in a murine model of
            lipodystrophy caused by adipose deficiency of vacuolar H+-ATPase
            V0d1 subunit
  JOURNAL   Theranostics 14 (5), 2246-2264 (2024)
   PUBMED   38505620
  REMARK    GeneRIF: Myocardin reverses insulin resistance and ameliorates
            cardiomyopathy by increasing IRS-1 expression in a murine model of
            lipodystrophy caused by adipose deficiency of vacuolar H[+]-ATPase
            V0d1 subunit.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8086)
  AUTHORS   Racedo,S.E., Liu,Y., Shi,L., Zheng,D. and Morrow,B.E.
  TITLE     Dgcr8 functions in the secondary heart field for outflow tract and
            right ventricle development in mammals
  JOURNAL   Dev Biol 506, 72-84 (2024)
   PUBMED   38110169
REFERENCE   4  (bases 1 to 8086)
  AUTHORS   Katano,W., Mori,S., Sasaki,S., Tajika,Y., Tomita,K., Takeuchi,J.K.
            and Koshiba-Takeuchi,K.
  TITLE     Sall1 and Sall4 cooperatively interact with Myocd and SRF to
            promote cardiomyocyte proliferation by regulating CDK and cyclin
            genes
  JOURNAL   Development 150 (24) (2023)
   PUBMED   38014633
  REMARK    GeneRIF: Sall1 and Sall4 cooperatively interact with Myocd and SRF
            to promote cardiomyocyte proliferation by regulating CDK and cyclin
            genes.
REFERENCE   5  (bases 1 to 8086)
  AUTHORS   Bharadhwaj,R.A. and Kumarswamy,R.
  TITLE     Long noncoding RNA TUG1 regulates smooth muscle cell
            differentiation via KLF4-myocardin axis
  JOURNAL   Am J Physiol Cell Physiol 325 (4), C940-C950 (2023)
   PUBMED   37642238
  REMARK    GeneRIF: Long noncoding RNA TUG1 regulates smooth muscle cell
            differentiation via KLF4-myocardin axis.
REFERENCE   6  (bases 1 to 8086)
  AUTHORS   Yoshida,T., Sinha,S., Dandre,F., Wamhoff,B.R., Hoofnagle,M.H.,
            Kremer,B.E., Wang,D.Z., Olson,E.N. and Owens,G.K.
  TITLE     Myocardin is a key regulator of CArG-dependent transcription of
            multiple smooth muscle marker genes
  JOURNAL   Circ Res 92 (8), 856-864 (2003)
   PUBMED   12663482
REFERENCE   7  (bases 1 to 8086)
  AUTHORS   Du,K.L., Ip,H.S., Li,J., Chen,M., Dandre,F., Yu,W., Lu,M.M.,
            Owens,G.K. and Parmacek,M.S.
  TITLE     Myocardin is a critical serum response factor cofactor in the
            transcriptional program regulating smooth muscle cell
            differentiation
  JOURNAL   Mol Cell Biol 23 (7), 2425-2437 (2003)
   PUBMED   12640126
  REMARK    GeneRIF: Myocardin is a critical serum response factor cofactor
            regulating smooth muscle cell differentiation; forced expression of
            myocardin activated expression of SM22 alpha, smooth muscle
            alpha-actin, and calponin-h1 genes in mouse ES cells
REFERENCE   8  (bases 1 to 8086)
  AUTHORS   Wang,D.Z., Li,S., Hockemeyer,D., Sutherland,L., Wang,Z.,
            Schratt,G., Richardson,J.A., Nordheim,A. and Olson,E.N.
  TITLE     Potentiation of serum response factor activity by a family of
            myocardin-related transcription factors
  JOURNAL   Proc Natl Acad Sci U S A 99 (23), 14855-14860 (2002)
   PUBMED   12397177
REFERENCE   9  (bases 1 to 8086)
  AUTHORS   Hauschka,S.D.
  TITLE     Myocardin. a novel potentiator of SRF-mediated transcription in
            cardiac muscle
  JOURNAL   Mol Cell 8 (1), 1-2 (2001)
   PUBMED   11511353
REFERENCE   10 (bases 1 to 8086)
  AUTHORS   Wang,D., Chang,P.S., Wang,Z., Sutherland,L., Richardson,J.A.,
            Small,E., Krieg,P.A. and Olson,E.N.
  TITLE     Activation of cardiac gene expression by myocardin, a
            transcriptional cofactor for serum response factor
  JOURNAL   Cell 105 (7), 851-862 (2001)
   PUBMED   11439182
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL669846.11.
            
            On Nov 16, 2022 this sequence version replaced NM_145136.4.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY303755.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-352               AL669846.11        98758-99109         c
            353-418             AL669846.11        62233-62298         c
            419-474             AL669846.11        52848-52903         c
            475-550             AL669846.11        51146-51221         c
            551-712             AL669846.11        47643-47804         c
            713-876             AL669846.11        33373-33536         c
            877-1002            AL669846.11        29908-30033         c
            1003-1295           AL669846.11        25216-25508         c
            1296-1449           AL669846.11        24182-24335         c
            1450-2346           AL669846.11        16033-16929         c
            2347-2490           AL669846.11        13602-13745         c
            2491-2619           AL669846.11        12474-12602         c
            2620-2677           AL669846.11        9999-10056          c
            2678-8086           AL669846.11        2722-8130           c
FEATURES             Location/Qualifiers
     source          1..8086
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 40.42 cM"
     gene            1..8086
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /note="myocardin"
                     /db_xref="GeneID:214384"
                     /db_xref="MGI:MGI:2137495"
     exon            1..352
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     CDS             298..3249
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /note="isoform A is encoded by transcript variant 1; SRF
                     co-factor protein (cardiac and smooth muscle); SRF
                     cofactor protein; basic SAP coiled-coil transcription
                     activator 2"
                     /codon_start=1
                     /product="myocardin isoform A"
                     /protein_id="NP_660118.3"
                     /db_xref="CCDS:CCDS48819.1"
                     /db_xref="GeneID:214384"
                     /db_xref="MGI:MGI:2137495"
                     /translation="MTLLGSEHSLLIRRKFRSVLQLRLQQRRTQEQLANQGLIPPLKG
                     PTEFHDPRKQLDSAKTEDSLRRKGRNRSDRASLVTMHILQASTAERSIPTAQMKLKRA
                     RLADDLNEKIALRPGPLELVEKNILPMDSSVKEAIKGTEVSLSKAADAFAFEDDSSRD
                     GLSPDQARSEDPQGSTGSTPDIKSTEAPLDTIQDLTPGSESDKNDAASQPGNQSDPGK
                     QVLGPLSTPIPVHTAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPP
                     PMDSAYARLLQQQQLFLQLQILSQQQQQQQQQQQQQQQQQQQQQRFSYPGMHQTHLKE
                     PNEQMARNPNPSSTPLSNTPLSPVKNSISGQTGVSSLKPGPLPPNLDDLKVSELRQQL
                     RIRGLPVSGTKTALVDRLRPFQDCAGNPVPNFGDITTVTFPVTPNTLPSYQSSPTGFY
                     HFGSTSSSPPISPASSDLSAAGSLPDTFTDASPGFGLHASPVPACTDESLLSSLNGGS
                     GPSEPDGLDSEKDKMLVEKQKVINQLTWKLRQEQRQVEELRMQLQKQKSSCSDQKPLP
                     FLATTIKQEDVSSCPFAPQQASGKGQGHSSDSPPPACETAQLLPHCVESSGQTHVLSS
                     TFLSPQCSPQHSPLGGLKSPQHISLPPSPNNHYFLASSSGAQRENHGVSSPSSSQGCA
                     QNSGAHEGHSSSFSSPASSLHQPFSGTQADSSHSAGLNPCPKSPSIHPKMTGLQSSDK
                     VGPTFSIPSPTFSKSSSAVSDITQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPAD
                     AREDHSCLQKIPKIPGSSCSPTAIPPKPSASFEQASSGGQMAFDHYANDSDEHLEVLL
                     NSHSPIGKVSDVTLLKIGSEEPPFDSIMDGFPGKAAEDLFSAHELLPGPLSPMHAQLS
                     PPSVDSSGLQLSFTESPWETMEWLDLTPPSSTPGFSNLTSSGPSIFNIDFLDVTDLNL
                     NSPMDLHLQQW"
     exon            353..418
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            419..474
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            475..550
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            551..712
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            713..876
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            877..1002
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            1003..1295
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1449
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            1450..2346
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            2347..2490
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            2491..2619
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            2620..2677
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     exon            2678..8086
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3417..3422
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /note="hexamer: ATTAAA"
     polyA_site      3438
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
     regulatory      8063..8068
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /note="hexamer: AATAAA"
     polyA_site      8086
                     /gene="Myocd"
                     /gene_synonym="BSAC2A; Srfcp"
                     /note="major polyA site"
ORIGIN      
        1 gaccgctggc agcctatgac atcagccagg aacgcctagg atgcggctgt tcccggccac
       61 cctcagagga ggagggtcct gcctgctggg agttaattag cctcgcgagc ggcgaggggg
      121 gaggcgccag ttttctgggg acactggcgg ccactgtgcg tcctcctacc caagggagct
      181 ccccaagagt tggatgaatt ctgggttgtt agctgctgtc ctctgggctc ccgggagcca
      241 gtttctggtg gaaagcgggg cgcctggcca acgaccagcg gcttgctgag actcaccatg
      301 acactcctgg ggtctgaaca ctctttgctg attagaagga agttccgatc agtcttacag
      361 ttacggcttc aacagagaag gacccaggag cagctggcta accaaggctt aataccgcca
      421 ctgaaaggtc caactgaatt ccatgacccg agaaaacaat tggatagtgc caagactgaa
      481 gattccctga ggcgcaaggg cagaaacagg tccgaccgtg ccagcctggt tactatgcac
      541 attctccaag cctccacggc agaaaggtcc attccaactg ctcagatgaa gctcaaaaga
      601 gcccgccttg cagatgacct caatgagaag atcgctctcc gcccagggcc cttggaactg
      661 gtggagaaga acattctgcc gatggattct tccgtgaaag aggctataaa aggtactgag
      721 gtgagcctct ccaaggcagc agatgcattc gcctttgagg atgacagcag tagagatggg
      781 ctctctccag atcaggctag gagcgaggac ccccagggct ctacaggatc caccccagac
      841 atcaaatcca ctgaggctcc tctggacaca atccaggatc tcactcctgg ctcagaaagt
      901 gacaagaatg atgcagcctc ccagccaggc aaccagtcag accctgggaa gcaggttctc
      961 ggccccctca gcaccccgat tcctgtgcac actgctgtaa agtccaagtc tttgggtgac
     1021 agtaagaacc gccacaaaaa gcccaaagac cccaaaccaa aggtgaagaa gctcaaatac
     1081 catcagtaca tccccccaga ccagaaggca gagaagtctc ccccacccat ggactctgcc
     1141 tatgcccggc tgctccagca acagcagcta ttcctgcagc tacagatcct cagccagcag
     1201 cagcaacagc agcagcaaca gcagcagcag caacagcagc agcagcagca gcagcagcgg
     1261 ttcagctacc ctgggatgca ccaaacacac ctcaaagaac caaatgaaca gatggccaga
     1321 aatccgaatc cttcttcaac accactgagc aatacccctc tatcccctgt caaaaatagc
     1381 atttctggac aaactggtgt ttcttctctc aaaccaggcc ccctcccacc caacctggat
     1441 gatctcaagg tgtcagagtt aagacaacag cttcgaatcc ggggcttgcc agtgtcaggc
     1501 accaagacag cgctggtgga ccggcttcgt cccttccagg attgtgctgg caaccctgtg
     1561 cccaactttg gggacatcac aactgtcacc tttcctgtca cgcccaacac cttgcccagt
     1621 tatcagtcct ccccgacagg cttctaccac tttggcagca caagctccag cccacccatc
     1681 tcccccgcct catctgactt gtccgctgca gggtccctgc cagacacctt caccgatgcg
     1741 tcacctggct tcggcctgca cgcatctccg gtgcccgcct gcacggacga gagtctgctg
     1801 agcagcctga atgggggctc gggcccctcc gagcctgatg ggctagactc tgagaaggac
     1861 aagatgctgg tggagaagca gaaagtgatc aaccagctca cctggaagct gcggcaagag
     1921 cagcggcagg tggaagagct gagaatgcaa ctgcagaagc agaagagcag ctgcagcgac
     1981 cagaagccac tgcccttctt ggccaccacc atcaaacagg aagatgtctc cagctgcccc
     2041 ttcgcacccc agcaggcgtc tgggaaggga cagggccaca gctctgacag tccccctccg
     2101 gcttgtgaga cggctcagct gctgcctcac tgtgtggagt cctcaggtca aacccatgta
     2161 ctctcgtcca cgtttctcag cccccagtgc tcccctcagc actcgcccct ggggggcctg
     2221 aagagcccgc agcacatcag cctgcctcca tcacccaaca accattactt cctggcttcc
     2281 tcttcgggag ctcagagaga gaaccatggg gtctcttcac ccagcagcag ccaagggtgc
     2341 gcacagaact caggggcaca cgaaggccat tcttctagct tctcttcccc agcttccagc
     2401 ctccatcagc ctttctctgg cacccaagca gacagcagtc acagtgctgg gctcaaccct
     2461 tgtcccaaaa gcccaagtat tcatccaaag atgactggtt tacaatcttc tgacaaggtg
     2521 gggccaacgt tttcaattcc atccccaact ttttctaagt caagttcagc agtttcagat
     2581 atcacccagc ccccatccta tgaagatgca gtgaagcagc aaatgactcg gagtcagcag
     2641 atggacgaac tcctggatgt cctcattgaa agtggagaaa tgccagccga tgccagggaa
     2701 gatcattcat gtcttcagaa aattccaaag atccctgggt cctcctgcag cccaactgcc
     2761 atccccccga agccctcggc ttcctttgag caggcatctt cgggaggcca gatggccttc
     2821 gatcactacg ccaacgacag tgacgaacac ctggaagtct tattgaattc tcacagcccc
     2881 atcggaaagg tgagcgatgt taccctcctc aaaatcggaa gcgaggagcc tccttttgac
     2941 agcatcatgg atggcttccc agggaaggct gcggaagatc tcttcagtgc tcacgagctc
     3001 ttgcctgggc ccctctcccc gatgcatgca cagttgtcac ctccttctgt ggacagcagt
     3061 ggtctgcagc tgagcttcac ggaatctcct tgggaaacaa tggaatggct ggacctcact
     3121 ccacctagtt ccacgccagg cttcagcaac cttacctcca gtgggcccag cattttcaac
     3181 atcgattttc tggatgttac agatcttaat ctgaattccc ctatggatct ccacttacag
     3241 cagtggtaaa cacccgaggt acaagagcta cgagagctca gtgggaattc aatggaggaa
     3301 agcacgatac cggaaatgtg tgttccaaaa gatgaagtgg ggaaaatggg gagggaaaaa
     3361 aaaaaacagc aacggaggtt tttgtgacaa ctaaccagaa caaacagaag tcagctatta
     3421 aaatatgtct aaatgtaata tctaccagca ttcagtaact gttaataact tcagtgatgc
     3481 attcaaaaat gtgctttgtc agaataagaa tgccaaaaat gttttttcgc tgccttatct
     3541 cataccagtt tttttggggt tttttttgtt tgtttgtttt ttggtttttt ttttttttgt
     3601 gttgttattt ggttttcttt ttgcccacag tttgtctcag gcaatactgg gacatagact
     3661 gaccccatta gcttttgtta tgaatttact aaactttctg tggaaggaga acagagcctc
     3721 tgccgcgggt gtggggaagc catcctgtgc ttgaggcagc acacgtgtgt ccatcatcat
     3781 cagtcagaag agcagggcct gtctcaccca atcgagtcct taagacagaa taatcagaat
     3841 ggtcagaggg acagaccaat caattcccag gaaagcaaaa gtgactcaat gtcccttgac
     3901 tcccaaatgg tcccactgga ctggtgatca ctggtgacaa ctaactagct ttgtccagag
     3961 aatccaccca gaacacggtg ctttttagcc agtagtccac ctctatgtgc atcagcaatg
     4021 catagcaggt gagaacttga atcacagaaa cttcatgcca tggatggaga ctcctgaggc
     4081 gctcaaatac tactacctct agttccaaag actagagcta gatgatcaga aaggcaactg
     4141 gaggcccagg gagccgtact gggacaagtt agaattagag aacgatgtca tttaacattc
     4201 cgagaaagaa ataaccatga attgctatta caggagtaac acacagggcc agcttctttt
     4261 tcttcttttt tatttttctt ttcttattgt gagcagaggg aattcacctc agttcatctt
     4321 tctctcagta cttttctttc aagatatcaa tcctttatga ctcttttgct tttaattctc
     4381 tctctctctc tctctctctc tctttctctc aaaggagagg tttcagttct aacaagctac
     4441 catactccta ttaaagccat tttttttttt agaatattaa aagtccaaac tctcttgcca
     4501 aactctttct tcacatgcgc attggctgaa aacagaattt acaagaattt ctttaggaag
     4561 aaactgggga tgtggcccat tggtcacaaa gtttttttgt ttgtttttgt ttttgtttca
     4621 attcttgttt gatttatgga caatctttgg tttgtattgc tctggagaaa ttggaaatca
     4681 ttgcagagtg aagataaatc agggcaccat gtatagtaga gaatatttca gtagttttcc
     4741 aaacgagaac acaattgcac actgtaaaca acaggagtgt gaaggaccac agtcttgagg
     4801 agttcttgtt gccctgcgtt tggtgaaggc gttggggacc gaggaagaca acatacagtt
     4861 tggccaaggc tctcagaggc ttgctgtggc gccaattcaa gtattacaat gttgcatgct
     4921 gtagaaagta gctgttgctg ttgttttgtt ttgttttaat ttaagtcacc aaggcactgt
     4981 tttattcttt tgtaaaaaaa aaaaaaagtt cactgtgcac ttatagagaa aataatcaac
     5041 aatgttgtga atttttgaga agactttttt tttttttttg ataaaccaaa gatttagaaa
     5101 tcattccatt gtcaacttgt aaaaaaaaaa aaatgtttga acactgagtg tttttggtga
     5161 ctgctactct ggaagccacc agatcttctg gtagggaagg aaacacagat gtgtacacac
     5221 atgtagttct atacatgtgc ctatggagta tgtctataca cacatacaca tatgtatgca
     5281 actatgtgtg tgtgcacata tctgaaaggt ggcattcaga gggcctccga ccctcagtcc
     5341 gctgtgaatg atgcggagcc ttttgtcact acgagttgct gattgatcaa ccagactcca
     5401 tgttgcctct ctgtatatga cttaggacgc caactcaatg ctcacagcca cgtgctgcag
     5461 gggaacaagc tcctcctgac aatgagtatg gatttgagag gacaaagccc ggcagtgagt
     5521 ttggatgtga tgggatagag tggcttcacc atcctgtggc tgccaaggct agggccttct
     5581 cttacagaag agtctaaagg gagtatgaac tttgttctga cattccttga tctgcgttgt
     5641 gcttgaaagg ctctgcgtga tacactttcc ctcgtgtttg tatgcaatca gaaccacaga
     5701 tccagagata aagcttagca tgaagatgaa gctgggggag gaaccttatt gccacattcc
     5761 aaacactggt ttgacctccg acgctgatgt tgcatttgag tctggatgaa gcttcctgct
     5821 ctgtacattt ttctttaggc tttggctgaa ccatgtatgt tatccccttc ttgcttcttt
     5881 ggaaaacaaa tccacacagt gtcttgcctc tgagaccttc tctgagtgct gataattttc
     5941 cctttgggta tccctgtctg agagattggt tagacagctc tgttttttag ttagactgtg
     6001 gggaactttt taaaatagta tccaaactga cacacacact tcacactaga cgaaagggaa
     6061 gatggctctg gtttgctagt gcaagccagt acagctagaa tgggcccctg gatacttggc
     6121 ctcagcttgc tcctgcaatc tccccaggcc atgtgcatat accctgtctt cacagcacag
     6181 gtagcagatt ggagtagctg tgagaagatt gatgaagacc gatcaaatac gtgccttcag
     6241 ctgtctccta ttgtggcttt taagttcctt ttattaatca gtcaagtcct cttgtggtgg
     6301 caatgtgaca agctaagagg tgtgcagaga tggtcaccct tgggtttagc aatgtttcta
     6361 gagcctcaag tgaccacata gttaggaggt ctgtaatatc cccatggtaa gctcagtttt
     6421 ggcagccatt cctactgtag ctacaggaca acaggtcctt ctagatctaa aggagctcag
     6481 attctcccca tgcaagttct tccagtaaac tagggttcag taattatgcc tgtgtcctaa
     6541 atgttatcaa gacaatgggg ttgaggggga aaagtgaaga agaaaggaga cagaggaaga
     6601 ggagggaggg aagaggaggg agtaagcaga tgggagagga ggagaaggag gggacagagg
     6661 aaggaaagaa gcaaggaagg aagagagagg gaatgaagga gagagagaag gagaaaggaa
     6721 ggaggcaggg aagcaaagga aggagaaaag aaaatcatga cccaacttct tgacccatga
     6781 aactcttggt tcagctatga cgccatagta caaaaagtaa aagacaagag tggaagacag
     6841 cacccattct gagcaatgga gtgattccct ctgcaatcag gagcctcaac acatcttcta
     6901 gtggaagagt cctatagacc tgcagcctgt gcctttcaca aacattagct ggggacaaga
     6961 acaattaaaa tctaccccaa gcttttaaaa caattcttct ccaaagctat ctattttatg
     7021 ttcaatcagc tttcctagag ctaataaggc cgaaaactcc ctcgtgaccc gtactaccca
     7081 gggcgtggcc atttggtgag cgccacagag cacacaaaac cttcaggggt tgattcagca
     7141 tgggtgttga acatctgagg gattgcagct cctctaagca agaatgctga gaccctctga
     7201 gactctctca gctctttgca ctcaaattgt cttagcagtg ataaagggct cttgattttt
     7261 ccagaaactg gagtttttgg ttttgctttt tttttttttt ttcttttcat gaaaagctac
     7321 tagcacacaa gcttgtctct tcttccaggg tcacaggaca gtagtgtagg ttcagaggca
     7381 gatgcttctg agacttccct agacagagac gtcaacagac atggaagaga cagtcccatt
     7441 gtgctgagaa cacggtcatg tgggtccaga ggccctcgct aggagaggag actctgtcag
     7501 gaaatgggtt caagacagga aggaatttga gaagaccttt gttgctgcct ggcagggata
     7561 ttgttctagg attcactgga ttcgatgagc acgaacgagc tcccttctga tcataaggaa
     7621 tcaatggtcc ccccacaagt aaccccctgc agggctctgg gtcccatgat gggcatcagg
     7681 aggccacact tcccctggat ggtttagatg gcctcacgtg caaaggtgca cagcaagggg
     7741 acccagaaac actcttttaa atgttttcca aaggagcaaa ccccacactg ctccaagggc
     7801 tctgttccaa tgaccttgtc aaaaggtcca agggcaaagg gcttctgaga gagcaactct
     7861 cactggaaca cgaagcagtg ttctgggaag aacagggaaa caagcatggt tctctcagcc
     7921 tctgcgtggt ggtgttttca tattggtctc ctttgctcag tatagtctga gagaaatgtg
     7981 gctcactggg agagcaaagg agcaatggct aaaataccaa agttggcatg tgtctttttt
     8041 taaaaaaatg catatatttt taaataaaat gtttatttta acgaga
//
