LOCUS       NM_001177468             808 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus myomixer, myoblast fusion factor (Mymx), transcript
            variant 1, mRNA.
ACCESSION   NM_001177468
VERSION     NM_001177468.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Cai,S., Wang,X., Xu,R., Liang,Z., Zhu,Q., Chen,M., Lin,Z., Li,C.,
            Duo,T., Tong,X., Li,E., He,Z., Liu,X., Chen,Y. and Mo,D.
  TITLE     KLF4 regulates skeletal muscle development and regeneration by
            directly targeting P57 and Myomixer
  JOURNAL   Cell Death Dis 14 (9), 612 (2023)
   PUBMED   37723138
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  AUTHORS   Dos Santos,M., Shah,A.M., Zhang,Y., Bezprozvannaya,S., Chen,K.,
            Xu,L., Lin,W., McAnally,J.R., Bassel-Duby,R., Liu,N. and Olson,E.N.
  TITLE     Opposing gene regulatory programs governing myofiber development
            and maturation revealed at single nucleus resolution
  JOURNAL   Nat Commun 14 (1), 4333 (2023)
   PUBMED   37468485
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 808)
  AUTHORS   Kurosaka,M., Hung,Y.L., Machida,S. and Kohda,K.
  TITLE     IL-4 Signaling Promotes Myoblast Differentiation and Fusion by
            Enhancing the Expression of MyoD, Myogenin, and Myomerger
  JOURNAL   Cells 12 (9), 1284 (2023)
   PUBMED   37174683
  REMARK    GeneRIF: IL-4 Signaling Promotes Myoblast Differentiation and
            Fusion by Enhancing the Expression of MyoD, Myogenin, and
            Myomerger.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 808)
  AUTHORS   Golani,G., Leikina,E., Melikov,K., Whitlock,J.M., Gamage,D.G.,
            Luoma-Overstreet,G., Millay,D.P., Kozlov,M.M. and Chernomordik,L.V.
  TITLE     Myomerger promotes fusion pore by elastic coupling between proximal
            membrane leaflets and hemifusion diaphragm
  JOURNAL   Nat Commun 12 (1), 495 (2021)
   PUBMED   33479215
  REMARK    GeneRIF: Myomerger promotes fusion pore by elastic coupling between
            proximal membrane leaflets and hemifusion diaphragm.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 808)
  AUTHORS   Suzuki,A., Minamide,R. and Iwata,J.
  TITLE     WNT/beta-catenin signaling plays a crucial role in myoblast fusion
            through regulation of nephrin expression during development
  JOURNAL   Development 145 (23) (2018)
   PUBMED   30389854
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 808)
  AUTHORS   Quinn,M.E., Goh,Q., Kurosaka,M., Gamage,D.G., Petrany,M.J.,
            Prasad,V. and Millay,D.P.
  TITLE     Myomerger induces fusion of non-fusogenic cells and is required for
            skeletal muscle development
  JOURNAL   Nat Commun 8, 15665 (2017)
   PUBMED   28569755
  REMARK    GeneRIF: myomerger is a fundamental myoblast fusion protein with
            roles in skeletal muscle development
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 808)
  AUTHORS   Zhang,Q., Vashisht,A.A., O'Rourke,J., Corbel,S.Y., Moran,R.,
            Romero,A., Miraglia,L., Zhang,J., Durrant,E., Schmedt,C.,
            Sampath,S.C. and Sampath,S.C.
  TITLE     The microprotein Minion controls cell fusion and muscle formation
  JOURNAL   Nat Commun 8, 15664 (2017)
   PUBMED   28569745
  REMARK    GeneRIF: Minion is a novel microprotein required for muscle
            development
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 808)
  AUTHORS   Bi,P., Ramirez-Martinez,A., Li,H., Cannavino,J., McAnally,J.R.,
            Shelton,J.M., Sanchez-Ortiz,E., Bassel-Duby,R. and Olson,E.N.
  TITLE     Control of muscle formation by the fusogenic micropeptide myomixer
  JOURNAL   Science 356 (6335), 323-327 (2017)
   PUBMED   28386024
  REMARK    GeneRIF: the Myomixer-Myomaker pair controls the critical step in
            myofiber formation during muscle development.
REFERENCE   9  (bases 1 to 808)
  AUTHORS   Lin,Q., Donahue,S.L., Moore-Jarrett,T., Cao,S., Osipovich,A.B. and
            Ruley,H.E.
  TITLE     Mutagenesis of diploid mammalian genes by gene entrapment
  JOURNAL   Nucleic Acids Res 34 (20), e139 (2006)
   PUBMED   17062627
REFERENCE   10 (bases 1 to 808)
  AUTHORS   Chen,Y.M., Du,Z.W. and Yao,Z.
  TITLE     Molecular cloning and functional analysis of ESGP, an embryonic
            stem cell and germ cell specific protein
  JOURNAL   Acta Biochim Biophys Sin (Shanghai) 37 (12), 789-796 (2005)
   PUBMED   16331322
  REMARK    GeneRIF: The expression of ESGP is consistent with the expression
            of Oct-4 during embryonic development.Forced expression of ESGP is
            not indispensable for the effect of Oct-4 on ES cell self-renewal,
            and does not affect the differentiation to three germ layers.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            W70601.1, CJ191178.1 and BB399187.1.
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes isoform 1. Variants 1 and 2 encode the same
            isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: DQ190000.1, AV582885.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849377
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-452               W70601.1           1-452
            453-801             CJ191178.1         191-539
            802-808             BB399187.1         266-272
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.59 cM"
     gene            1..808
                     /gene="Mymx"
                     /gene_synonym="EG653016; Esgp; Gm7325; minion; myomerger"
                     /note="myomixer, myoblast fusion factor"
                     /db_xref="GeneID:653016"
                     /db_xref="MGI:MGI:3649059"
     exon            1..89
                     /gene="Mymx"
                     /gene_synonym="EG653016; Esgp; Gm7325; minion; myomerger"
                     /inference="alignment:Splign:2.1.0"
     exon            90..808
                     /gene="Mymx"
                     /gene_synonym="EG653016; Esgp; Gm7325; minion; myomerger"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..358
                     /gene="Mymx"
                     /gene_synonym="EG653016; Esgp; Gm7325; minion; myomerger"
                     /note="isoform 1 is encoded by transcript variant 1;
                     embryonic stem cell- and germ cell-specific protein;
                     protein minion; protein myomerger; microprotein inducer of
                     fusion; protein myomixer"
                     /codon_start=1
                     /product="protein myomixer isoform 1"
                     /protein_id="NP_001170939.1"
                     /db_xref="CCDS:CCDS50119.1"
                     /db_xref="GeneID:653016"
                     /db_xref="MGI:MGI:3649059"
                     /translation="MPVPLLPMVLRSLLSRLLLPVARLARQHLLPLLRRLARRLSSQD
                     MREALLSCLLFVLSQQQPPDSGEASRVDHSQRKERLGPQK"
     regulatory      780..785
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mymx"
                     /gene_synonym="EG653016; Esgp; Gm7325; minion; myomerger"
                     /note="hexamer: AATAAA"
     polyA_site      804
                     /gene="Mymx"
                     /gene_synonym="EG653016; Esgp; Gm7325; minion; myomerger"
                     /note="major polyA site"
ORIGIN      
        1 gaaattgatt ctgagcagtt ctgactggtg agagctgcca ctggccggtt agaactggtg
       61 agcaggaggg caagaagttc aggcttcagg tgcaggtcct gccatgcccg ttccattgct
      121 cccgatggtg cttcgatcgc tgctgtcccg cctgctgctg cctgttgccc gcctggcccg
      181 gcagcacctc ctgcccttgc tgcgccggct ggcccgccga ctgagctccc aagacatgag
      241 agaggctctg ctgagctgtc tgctctttgt cctcagccag caacagccac cggattctgg
      301 agaggcctcc agagtggacc actcccagag gaaggagaga ttgggccccc agaagtgagg
      361 ccacgggtcc tggaaacagc aacgcccatc aaagtacttt tggagccggt tagtccaggc
      421 gtcggtccgc acgcacgggc atggacggca gactgcccag tgggcgaaga cagtccgggc
      481 tgagtgcaag agggctctga cctgaacagt tcccactccc ctagctccta gcaggctaca
      541 gattgtgttg attgaccctt cctctctgca gctccctctg ccttatctct ggcctccagg
      601 gtggacctgc aaatgcgggt atcaaggtca gttaagagat gatgtttcac ttagactcaa
      661 gacaatttag ctaagaggtg gtatttaaat ccaaactgtc ccgtctacct taaaattata
      721 agcccttgct tcccgttaaa gaatgagtac tttcaaagct tggtacgcag caggtagtca
      781 ataaacgagg acagtgtgtt gttattgg
//
