LOCUS       NM_025376               1401 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus myomaker, myoblast fusion factor (Mymk), transcript
            variant 1, mRNA.
ACCESSION   NM_025376
VERSION     NM_025376.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Dos Santos,M., Shah,A.M., Zhang,Y., Bezprozvannaya,S., Chen,K.,
            Xu,L., Lin,W., McAnally,J.R., Bassel-Duby,R., Liu,N. and Olson,E.N.
  TITLE     Opposing gene regulatory programs governing myofiber development
            and maturation revealed at single nucleus resolution
  JOURNAL   Nat Commun 14 (1), 4333 (2023)
   PUBMED   37468485
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1401)
  AUTHORS   Hindi,S.M., Petrany,M.J., Greenfeld,E., Focke,L.C., Cramer,A.A.W.,
            Whitt,M.A., Khairallah,R.J., Ward,C.W., Chamberlain,J.S.,
            Podbilewicz,B., Prasad,V. and Millay,D.P.
  TITLE     Enveloped viruses pseudotyped with mammalian myogenic cell fusogens
            target skeletal muscle for gene delivery
  JOURNAL   Cell 186 (10), 2062-2077 (2023)
   PUBMED   37075755
  REMARK    Erratum:[Cell. 2023 Aug 3;186(16):3520. doi:
            10.1016/j.cell.2023.06.025.. PMID: 37541201]
REFERENCE   4  (bases 1 to 1401)
  AUTHORS   Cramer,A.A.W., Prasad,V., Eftestol,E., Song,T., Hansson,K.A.,
            Dugdale,H.F., Sadayappan,S., Ochala,J., Gundersen,K. and
            Millay,D.P.
  TITLE     Nuclear numbers in syncytial muscle fibers promote size but limit
            the development of larger myonuclear domains
  JOURNAL   Nat Commun 11 (1), 6287 (2020)
   PUBMED   33293533
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1401)
  AUTHORS   Petrany,M.J., Song,T., Sadayappan,S. and Millay,D.P.
  TITLE     Myocyte-derived Myomaker expression is required for regenerative
            fusion but exacerbates membrane instability in dystrophic myofibers
  JOURNAL   JCI Insight 5 (9), 136095 (2020)
   PUBMED   32310830
  REMARK    GeneRIF: Myocyte-derived Myomaker expression is required for
            regenerative fusion but exacerbates membrane instability in
            dystrophic myofibers.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1401)
  AUTHORS   Millay,D.P., Gamage,D.G., Quinn,M.E., Min,Y.L., Mitani,Y.,
            Bassel-Duby,R. and Olson,E.N.
  TITLE     Structure-function analysis of myomaker domains required for
            myoblast fusion
  JOURNAL   Proc Natl Acad Sci U S A 113 (8), 2116-2121 (2016)
   PUBMED   26858401
  REMARK    GeneRIF: data indicate that the majority of myomaker is embedded in
            the plasma membrane with seven membrane-spanning regions and a
            required intracellular C-terminal tail.
REFERENCE   7  (bases 1 to 1401)
  AUTHORS   Millay,D.P., Sutherland,L.B., Bassel-Duby,R. and Olson,E.N.
  TITLE     Myomaker is essential for muscle regeneration
  JOURNAL   Genes Dev 28 (15), 1641-1646 (2014)
   PUBMED   25085416
  REMARK    GeneRIF: myogenic basic helix-loop-helix (bHLH) transcription
            factors induce myomaker expression in satellite cells during acute
            and chronic muscle regeneration.
REFERENCE   8  (bases 1 to 1401)
  AUTHORS   Millay,D.P., O'Rourke,J.R., Sutherland,L.B., Bezprozvannaya,S.,
            Shelton,J.M., Bassel-Duby,R. and Olson,E.N.
  TITLE     Myomaker is a membrane activator of myoblast fusion and muscle
            formation
  JOURNAL   Nature 499 (7458), 301-305 (2013)
   PUBMED   23868259
  REMARK    GeneRIF: The findings reveal myomaker (transmembrane protein 8c) as
            a long-sought myogenic fusion protein that controls mammalian
            myoblast fusion.
REFERENCE   9  (bases 1 to 1401)
  AUTHORS   Gyurjan,I., Sonderegger,B., Naef,F. and Duboule,D.
  TITLE     Analysis of the dynamics of limb transcriptomes during mouse
            development
  JOURNAL   BMC Dev Biol 11, 47 (2011)
   PUBMED   21801425
  REMARK    Publication Status: Online-Only
REFERENCE   10 (bases 1 to 1401)
  AUTHORS   Kuninger,D., Kuzmickas,R., Peng,B., Pintar,J.E. and Rotwein,P.
  TITLE     Gene discovery by microarray: identification of novel genes induced
            during growth factor-mediated muscle cell survival and
            differentiation
  JOURNAL   Genomics 84 (5), 876-889 (2004)
   PUBMED   15475267
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB839715.1, AK003298.1 and BC062145.1.
            
            On Apr 23, 2009 this sequence version replaced NM_025376.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062145.1, AK003298.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-19                BB839715.1         3-21
            20-1200             AK003298.1         22-1202
            1201-1401           BC062145.1         1127-1327
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 19.21 cM"
     gene            1..1401
                     /gene="Mymk"
                     /gene_synonym="1110002H13Rik; myomaker; Tmem8c"
                     /note="myomaker, myoblast fusion factor"
                     /db_xref="GeneID:66139"
                     /db_xref="MGI:MGI:1913389"
     exon            1..281
                     /gene="Mymk"
                     /gene_synonym="1110002H13Rik; myomaker; Tmem8c"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..812
                     /gene="Mymk"
                     /gene_synonym="1110002H13Rik; myomaker; Tmem8c"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein myomaker; transmembrane protein 8C; myoblast
                     fusion maker"
                     /codon_start=1
                     /product="protein myomaker isoform 1"
                     /protein_id="NP_079652.1"
                     /db_xref="CCDS:CCDS15823.1"
                     /db_xref="GeneID:66139"
                     /db_xref="MGI:MGI:1913389"
                     /translation="MGTVVAKLLLPTLSSLAFLPTVSIATKRRFYMEAMVYLFTMFFV
                     AFSHACDGPGLSVLCFMRRDILEYFSIYGTALSMWVSLMALADFDEPQRSTFTMLGVL
                     TIAVRTFHDRWGYGVYSGPIGTATLIIAVKWLKKMKEKKGLYPDKSIYTQQIGPGLCF
                     GALALMLRFFFEEWDYTYVHSFYHCALAMSFVLLLPKVNKKAGNAGAPAKLTFSTLCC
                     TCV"
     exon            282..396
                     /gene="Mymk"
                     /gene_synonym="1110002H13Rik; myomaker; Tmem8c"
                     /inference="alignment:Splign:2.1.0"
     exon            397..545
                     /gene="Mymk"
                     /gene_synonym="1110002H13Rik; myomaker; Tmem8c"
                     /inference="alignment:Splign:2.1.0"
     exon            546..662
                     /gene="Mymk"
                     /gene_synonym="1110002H13Rik; myomaker; Tmem8c"
                     /inference="alignment:Splign:2.1.0"
     exon            663..1364
                     /gene="Mymk"
                     /gene_synonym="1110002H13Rik; myomaker; Tmem8c"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1303..1308
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mymk"
                     /gene_synonym="1110002H13Rik; myomaker; Tmem8c"
                     /note="hexamer: AGTAAA"
     polyA_site      1325
                     /gene="Mymk"
                     /gene_synonym="1110002H13Rik; myomaker; Tmem8c"
                     /note="major polyA site"
ORIGIN      
        1 gagcacttaa gccctccttg tgggtgctgc cacagctgcg gtcagggctg ctggagaagc
       61 aaagaagtgg gctctttttc ttcctgccct ggagaccagg ggggccttta ccaccttctc
      121 cccagtcagt gccttcctcc tggtccatgg ggacagttgt agccaaactg ctcctgccta
      181 ccctcagcag cctggccttc ctcccgacag tgagcatcgc taccaagagg cgtttctaca
      241 tggaggccat ggtctacctc ttcaccatgt tctttgtggc gttctcccat gcctgtgatg
      301 ggcctggttt gtctgtgctg tgcttcatgc gccgtgacat tctggagtac ttcagcatct
      361 atggaacagc cctgagcatg tgggtctccc tgatggcact ggccgacttt gatgaacccc
      421 agagatcgac cttcacaatg cttggcgtcc ttaccatcgc tgtgcggact tttcatgacc
      481 gctggggtta cggggtatac tccggtccca taggcacggc caccctcatc attgctgtaa
      541 agtggctgaa gaagatgaaa gagaagaagg gcctgtaccc cgacaagagc atctacaccc
      601 agcagatagg ccccggcctg tgctttgggg ccctggccct gatgcttcga ttcttctttg
      661 aggaatggga ttacacctac gtccacagct tctaccactg tgccctggcc atgtcctttg
      721 tcctgctgct gcccaaggtc aacaagaagg ctgggaacgc aggggccccc gccaagctga
      781 ccttctccac cctctgctgc acttgtgtct gactataccc ccccacacac acacacacac
      841 caggcccctg ccttcctgcc tggcagtcct gctgtctctc ccaaggtact tcctatactt
      901 tgttatgcgg cctgtacatg agaaatggtc ttctctacac cccaagagac cagcaggcct
      961 gctgcattct gctgagtgct gcttagggac ccactggttc tgtgttcacc agttgcttca
     1021 ctctgttcag gaaaaaaaag aactttatcc cccaaggcct cacaaccata ggtgtgcctg
     1081 gcagagaacc ctagaccagt aaatacccag cagcatgcag ggttatctat ttcccaggtc
     1141 ctgcctgtca gaatcgtctg ctttccctag gaaactggga ttgccctttt aacccctgcc
     1201 caggctctga aagctctcca cttaggaagc tggagccagc gaaccttgca tacccctgcc
     1261 tgagtcccat cccttctgca ggttttcgat caagccattc acagtaaact cttgatcagc
     1321 cactagtcat tagcttcgcc taacatacat tctagttcct ggaaaaaaaa aaaaaaaaaa
     1381 aaaaaaaaaa aaaaaaaaaa a
//
