LOCUS       NM_001368880            6590 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus myosin light chain kinase family, member 4 (Mylk4),
            transcript variant 1, mRNA.
ACCESSION   NM_001368880
VERSION     NM_001368880.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Sakakibara,I., Yanagihara,Y., Himori,K., Yamada,T., Sakai,H.,
            Sawada,Y., Takahashi,H., Saeki,N., Hirakawa,H., Yokoyama,A.,
            Fukada,S.I., Sawasaki,T. and Imai,Y.
  TITLE     Myofiber androgen receptor increases muscle strength mediated by a
            skeletal muscle splicing variant of Mylk4
  JOURNAL   iScience 24 (4), 102303 (2021)
   PUBMED   33870126
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6590)
  AUTHORS   Chang,A.N., Mahajan,P., Knapp,S., Barton,H., Sweeney,H.L.,
            Kamm,K.E. and Stull,J.T.
  TITLE     Cardiac myosin light chain is phosphorylated by
            Ca2+/calmodulin-dependent and -independent kinase activities
  JOURNAL   Proc Natl Acad Sci U S A 113 (27), E3824-E3833 (2016)
   PUBMED   27325775
  REMARK    GeneRIF: In contrast to smooth muscle MLCK and skeletal muscle
            MLCK, which lack activity in the absence of Ca(2+)/calmodulin,
            cardiac MLCK has constitutive activity that is stimulated by
            Ca(2+)/calmodulin.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645808.13.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223970.15877.1,
                                           SRR10223971.13242.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               AL645808.13        102313-102498       c
            187-457             AL645808.13        100092-100362       c
            458-536             AL645808.13        47610-47688         c
            537-957             AL645808.13        46869-47289         c
            958-1247            AL645808.13        45377-45666         c
            1248-1353           AL645808.13        43313-43418         c
            1354-1447           AL645808.13        40677-40770         c
            1448-1557           AL645808.13        40492-40601         c
            1558-1699           AL645808.13        38994-39135         c
            1700-1770           AL645808.13        36084-36154         c
            1771-1899           AL645808.13        35190-35318         c
            1900-2052           AL645808.13        34393-34545         c
            2053-2131           AL645808.13        31292-31370         c
            2132-2198           AL645808.13        27414-27480         c
            2199-6590           AL645808.13        19358-23749         c
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 13.72 cM"
     gene            1..6590
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /note="myosin light chain kinase family, member 4"
                     /db_xref="GeneID:238564"
                     /db_xref="MGI:MGI:3643758"
     exon            1..186
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            187..457
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     CDS             299..2173
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /EC_number="2.7.11.1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sgK085; sugen kinase 85"
                     /codon_start=1
                     /product="myosin light chain kinase family member 4
                     isoform 1"
                     /protein_id="NP_001355809.1"
                     /db_xref="GeneID:238564"
                     /db_xref="MGI:MGI:3643758"
                     /translation="MLKVKRLEEISSCHSSNPLEKVAFFQCMEEVEKVKCFLEENSGD
                     LDLQSGDNEAEENVWSNRALDERIIVKGGRTSALTEKTDQNDQFSSEKEKITKLNKKN
                     SEKTSALEVQRSCVICHVERQDKIEFVQRNSTELRENAGAKDAPCIESLEKQESNNLR
                     DKQDTEDNNGGENGISSKDDSRGVEAENRASAAASGAVLDKHSTESQHTEDMNGDPSH
                     ERCISNIKTTSTPGILGNSASGSTFQNAEQTESTGSSKRRVAEECFLSEDNKKSRIDV
                     VRRGEEPTARTEETRQARSLGDRLQDGMTTSREQDLEVPLDDIPAPAAPFDHRMVMAK
                     HASVDNLYTVSKSEILGGGRFGQVHKCEEKATGLKLAAKIIKTRGAKDKEDVKNEISV
                     MNQLDHVNLIQLYDAFESKHDIILVMEYVEGGELFDRIIDENCNLTELDTILFMKQIC
                     EGIRYMHQMYILHLDLKPENILCVNRDAKQIKIIDFGLARRYKPREKLKVNFGTPEFL
                     APEVVNYDFVSFSTDMWSVGVITYMLLSGLSPFLGDNDAETLTNILACRWDLEDEEFQ
                     DISEEAKEFISKLLIKEKSWRISASEALKHPWLSDHKLHSRLSAQKNCNSGVLNLTTK
                     "
     exon            458..536
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            537..957
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1247
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            1248..1353
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            1354..1447
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1557
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..1699
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            1700..1770
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            1771..1899
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2052
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            2053..2131
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            2132..2198
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     exon            2199..6590
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6560..6565
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /note="hexamer: AATAAA"
     polyA_site      6584
                     /gene="Mylk4"
                     /gene_synonym="EG238564"
                     /note="major polyA site"
ORIGIN      
        1 agggaagaca cttcctctgg tggtagacac ttgccttgct agatgacttc tgcccactga
       61 gagtgaagtc tcttactgca gagtcctgca gtccctctga attctgcaga tccctgttat
      121 cggccgaaaa gaatcctcta cttgcggagt gagtgggtca gactccagga tgtcctccta
      181 cagatgtcaa gagaagggag tgctgagcag tcatgattgt gtcctcattt acaaatctga
      241 gacagaggga gatgaaagcc ccactcctga cagcagcctg atcaggctca ccaccagtat
      301 gttgaaggtg aagaggctgg aagaaatcag ctcgtgccac agcagcaacc cgctggagaa
      361 ggtggcgttt ttccagtgca tggaggaggt ggagaaggtg aaatgcttcc tggaggagaa
      421 ttcgggggat ctggacctgc agtctggaga caatgaggct gaggaaaatg tatggtccaa
      481 ccgtgcacta gatgaaagga taattgtcaa aggaggaagg acatcagccc tcacagagaa
      541 aactgaccag aatgaccaat tctcttccga gaaagaaaag attaccaaac ttaacaagaa
      601 aaattcagag aagaccagtg cattagaagt gcagaggagt tgtgtcatat gtcacgtgga
      661 aagacaagac aagatagaat ttgttcagag gaattccaca gagctaagag agaatgcagg
      721 tgcaaaagat gctccatgca tagaaagtct ggagaaacaa gaaagcaaca acctgagaga
      781 taaacaagac actgaagata acaatggagg agaaaatggg atttcttcta aggacgattc
      841 cagaggagta gaagctgaga acagagcttc agcagctgca agtggagcag tgctagacaa
      901 acactcgact gaaagccagc acactgagga catgaatggt gacccgtccc atgaaaggtg
      961 catcagtaat ataaagacca cttctacacc aggaatcctt ggaaattcag catcaggatc
     1021 cactttccag aatgctgaac agacagagtc tactggcagc agtaagcgca gggtggcaga
     1081 ggagtgcttc ctcagtgagg acaacaaaaa gagcagaata gatgtagtga gaagagggga
     1141 agagcccaca gctaggacag aggaaaccag gcaagccaga agcctgggtg atagactcca
     1201 ggatggaatg accacctcta gagagcaaga cctggaggtc ccactggatg acatccctgc
     1261 tcctgccgct ccatttgatc atcggatggt aatggccaaa catgcttcag tggacaatct
     1321 gtacaccgtg agcaaatctg agatcctagg aggggggcgt tttggtcagg tacataagtg
     1381 tgaagagaaa gcaacaggcc tgaagctggc tgctaaaatc attaagacta gaggtgcaaa
     1441 ggacaaggag gatgtgaaga acgagatcag cgtcatgaac cagctggacc acgtgaacct
     1501 cattcagctc tatgatgcct ttgagtccaa gcatgacatc atccttgtca tggagtatgt
     1561 ggagggtggg gagctctttg accgcatcat tgatgaaaac tgcaacttga cagagctgga
     1621 caccatcctg ttcatgaagc agatctgtga ggggatacgg tacatgcacc agatgtacat
     1681 cctccacttg gatctcaagc ctgagaacat cctgtgtgtg aatcgggatg ctaagcaaat
     1741 aaaaattatt gattttggat tggccagaag atacaagccc agagagaagc tgaaggtgaa
     1801 ctttggaacc ccagaatttc ttgcccctga agttgtgaac tatgatttcg tttccttttc
     1861 tacagatatg tggagtgtgg gcgtcatcac atacatgcta ctcagtggct tgtccccctt
     1921 tttgggagac aatgatgccg agacactgac caacatcctg gcgtgccggt gggatttaga
     1981 agatgaggaa tttcaggata tctcggagga ggcaaaggag ttcatctcca agctcctgat
     2041 taaggagaag agttggagaa taagtgccag cgaagctctc aagcacccat ggttgtcaga
     2101 ccacaagctc cactccaggc tgagtgcaca gaagaactgc aactctggtg ttctgaacct
     2161 cacgaccaaa taatccatga aaggagccta ttcaaaagga aaactgctgc ggttgctgct
     2221 gctttgagaa ggcttttgga aaaatcagca gttctggtgc cttgacccct gggatgactg
     2281 gcagccatag catgggcagc cttgaacttg aacttggaaa ggctttgctg tgctcagtgg
     2341 gtgacccagg gctgctccct aggggcccag gcagatgccc acatcccact tagtgttcag
     2401 aggggaagat gtttctctgc ctttttggct tcttgtgttt caatgtttgt ttttagtttg
     2461 ggatgttagt ggggcagcgg atatgttagg ctgtttcttg gactaccaga gtggtgctgg
     2521 agacacaaaa tctggaggat gcattggagg agagagaacg acttctagtc cttgcactat
     2581 gtttctcaag tgggcaattg tattgacaac aaaattctaa gatttggacc catttttttt
     2641 tgtggtttga aatgaagtga attcaatgga ttattagctt gctggaacat aaggcttctt
     2701 tctaaggcaa gatcagccat cacatgtggt accttccatg cattctgaac tctctctctc
     2761 tctctctctc tctctctctc tctctctctc tctctctctc tttatctctc tctctctctc
     2821 tctctctctc tctctctcaa gtgcaacagt ttctacccag tagctgattc catgttcaga
     2881 ttcattcccc accattttac agggttcccc ttgctctgat ctaatgtgca agttcatccc
     2941 cactaaacct ggtggtttga ggaggtacat atttgtgctt agtctttaaa atgacaacct
     3001 tcaaaagata gccataagag cttccctcaa gacctaggtg tcaccagaag gagctgcgtc
     3061 tgtgcatcca gggaattaca atgtgcagtg ttcaggggca gtgtctagaa agtgactcca
     3121 gcctctgggt aaatggaaac acttcaaaga tcattaatca tagctacaca aattcaaaac
     3181 tatgcaaact cccataggag tcaatggctc taagggtaag gcaaaaccta tttctgatta
     3241 caaacatttt atttttttga gacgaggtcc taattataaa gccttggcgg gcctgaaatt
     3301 tgctatgtag atcaagctgg cctcaaactc atagaaaccc attcatatct actctagaat
     3361 gctgggattg aagacacacc actactatgc ctggaattgg ttgcaaagta ttatttccaa
     3421 gctcatgggc tatttgctgt cagatatggg atatatgagc accactgtag gtagccttta
     3481 gggaacagga tggcttgggg caagactttg caaaactccc tgaaattcca ctgacattca
     3541 ggctcttatt tcccaatggc ctttgagttt agttcagcca aacaatgccc ccagttttag
     3601 ccataaattg atgtcacaat tgtaatggta agaatagcca agtggccctg tcacccgggg
     3661 ctgacttgag acccttgact aactgccaaa ccctcatgtc cataagcatg cccaccaata
     3721 gctaacacct cctcattgcc ctttcatgcc ctgccccaaa gtaaagggta agtaggtacc
     3781 atttagtgaa ggccagtatc atttcactgt tgtgctatgt gtaaaactct gcttgttcca
     3841 accagcgttc ccaggctgcg acagacacct gctcctttct tttggctcat gtgtcatcca
     3901 aggttggttt ttaatacccc cctttaagct taaaattctt cttcttcttc ttcttcttct
     3961 tcttcttctt cttcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttcc
     4021 tcttcttcct cttcttcctc ttcttcctct tcttcctctt cctcctcctc ctcctccttc
     4081 tcctcctcct cctcttcttc ttcttcttct tcttcttctt cttcttcttc ttcttcttcc
     4141 tcctcctcct cttcctcctc ctcctcctcc tcctcctcct cctcctccac cccttcttcc
     4201 tattcctctt cttcatcctt ctcttcctcc tcctcttatt ttcttaataa aaaaagatac
     4261 acacacacat acacactcac aagctgggac aggtggagaa gctctgactt caatgttcta
     4321 ttactgtgct aagcttagcc gatgctcttc actgagcact cggaaaaaac acaagccagt
     4381 acacacatta atcctccttg accacagcag gctggtactc actcaaatgc taaatgggac
     4441 acaaaggtct ggttgaaaac gctgcataga aaggcatttt cagttgcggg catggattct
     4501 ccctgagcct ggggaagaga tcttcagcaa gacatgcacc ttttattcta actcacattg
     4561 ctatacatct ggacctgcca caagtggaaa ggacagctta ggtcacatac aggcaagagt
     4621 tcttggccac ctgaatttcc acaatacccc acaattagtg ggtgtgattg ctttagtttg
     4681 tgtttttgga ggtttagaga ggagactgct ttgagagtgg atcgtcgcat gggcagttaa
     4741 gcttgcacat ttggatagga aggtgggtac cccgctccag caagatcgtt ttatgcacaa
     4801 gctgaggcga gttcagtcgc caagttgagg attacaggct gagttacagt gctgcaggag
     4861 atggttagtc tggctttgtc tgtcccctcg atccacaccc acgggaggct gctcagtgaa
     4921 agcctatgat gtcggctctg atgggaaagg ggctttgcaa agctggccaa tcaatgcaga
     4981 tgccagctct gtggccccag tcatctactt ttccaagagg tcatcgggat ttggggtgct
     5041 gtcttcttct ccacgctctt tgggcacaag aggcttctta gttctaccaa agctgcttgg
     5101 agccgcagca catgtgctgt ttagaaagaa agagcctgaa gatagctgag aagggtgtcc
     5161 ttgtccttgt ctttggaaga tgctaacact tacccagaaa cagttgatgt tagagaacat
     5221 ggaggagaga gctcatgctc ccatttgctc cataaaaaaa attggaataa tttctgctct
     5281 aagatagctt agggcaaagc tttacaggtt aaattaggta acacatatgt ggcgagaccc
     5341 atgagtttat tttttggtgg ctttttgccc aactgatatt aatttatttt ctttctttaa
     5401 aatgtaaata ggcatgtgta ctttagtgtt ttgaagaaca tgtgaatagt tatggaaatt
     5461 agcatagtca attttcttgt tttggaatca aaatatggat tttatactaa ggaaatgagg
     5521 gttatgctct taattcattc cttcttctct gagtgctccc tgtcacgccc tccctgagca
     5581 aggggtgcag tgtgcataca ttttcttgtt tagtttgggc tctaggccag cttgcttgga
     5641 aaatttattt gtaaagtata aataaggaaa ggcagggcct gtgggctgtg gtgggtaggc
     5701 tcagctctgc catccctgtg ggccatgctc cagagctcag acaatgtgtt tgtttttaat
     5761 tttttcttca cttatttctt tttaagttag aacttagttc atcttcctca ttatgatatt
     5821 cctctaagag ccagtcagcc tgtcttcctt tttccttcct tcctccctcc ttcccttcct
     5881 ctaccccctc tctcctccct ccctccctac cttgcttcct tttttccttc cttgcttcct
     5941 tgcttccttc cttcaatcct tcctttcttc tttccttcct tccttctttc ttttcatgct
     6001 gggaatggac cccaggaacg tgtacacact aagcacatgt tataccactg tgaccttttt
     6061 gagatgagaa tttcttttcc aaatggcaaa gtgccttcag tggatgggtg atactgggtt
     6121 taagattcac accatgtcct tgtgcaactt gaagtctgca gtttatcaaa ttagaagtgc
     6181 cctacatatc tttttgctcc attctggtga agtgtgtttc tataaatgat ctttatatat
     6241 tgaaggctta agcacattca aaagaacaac agatacctaa cccgtgtgtg gagaagttgg
     6301 acatgtgtgc attttgcttt caccctagct gacagatgtc agtgtgtcct tctgaagagg
     6361 ttcactcatg cagatcagaa ttaaaagtgg attgtgtagt gactgtcatg tatattttta
     6421 tttctgaacg tagtaggaac atttggcttt acttctttgc cttatgaacg tgcactgtaa
     6481 gtcatatatg tgtattatat taaagaacta tattattttc tttgttgtta tttatgtttg
     6541 ttagtgactt aaattattca ataaagaagc cttggctaac acaacacaga
//
