LOCUS       NM_175441               3070 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus myosin light chain kinase 3 (Mylk3), transcript
            variant 1, mRNA.
ACCESSION   NM_175441 XM_912175
VERSION     NM_175441.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Hitsumoto,T., Tsukamoto,O., Matsuoka,K., Li,J., Liu,L.,
            Kuramoto,Y., Higo,S., Ogawa,S., Fujino,N., Yoshida,S., Kioka,H.,
            Kato,H., Hakui,H., Saito,Y., Okamoto,C., Inoue,H., Hyejin,J.,
            Ueda,K., Segawa,T., Nishimura,S., Asano,Y., Asanuma,H., Tani,A.,
            Imamura,R., Komagawa,S., Kanai,T., Takamura,M., Sakata,Y.,
            Kitakaze,M., Haruta,J.I. and Takashima,S.
  TITLE     Restoration of Cardiac Myosin Light Chain Kinase Ameliorates
            Systolic Dysfunction by Reducing Superrelaxed Myosin
  JOURNAL   Circulation 147 (25), 1902-1918 (2023)
   PUBMED   37128901
  REMARK    GeneRIF: Restoration of Cardiac Myosin Light Chain Kinase
            Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin.
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Hu,T., Kalyanaraman,H., Pilz,R.B. and Casteel,D.E.
  TITLE     Phosphatase regulatory subunit MYPT2 knockout partially compensates
            for the cardiac dysfunction in mice caused by lack of myosin light
            chain kinase 3
  JOURNAL   J Biol Chem 299 (4), 104584 (2023)
   PUBMED   36889588
REFERENCE   3  (bases 1 to 3070)
  AUTHORS   Kitazawa,T., Matsui,T., Katsuki,S., Goto,A., Akagi,K., Hatano,N.,
            Tokumitsu,H., Takeya,K. and Eto,M.
  TITLE     A temporal Ca2+ desensitization of myosin light chain kinase in
            phasic smooth muscles induced by CaMKKbeta/PP2A pathways
  JOURNAL   Am J Physiol Cell Physiol 321 (3), C549-C558 (2021)
   PUBMED   34106787
  REMARK    GeneRIF: A temporal Ca(2+) desensitization of myosin light chain
            kinase in phasic smooth muscles induced by CaMKKbeta/PP2A pathways.
REFERENCE   4  (bases 1 to 3070)
  AUTHORS   Williams,J.L., Paudyal,A., Awad,S., Nicholson,J., Grzesik,D.,
            Botta,J., Meimaridou,E., Maharaj,A.V., Stewart,M., Tinker,A.,
            Cox,R.D. and Metherell,L.A.
  TITLE     Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null
            C57BL/6J mice do not
  JOURNAL   Life Sci Alliance 3 (4), e201900593 (2020)
   PUBMED   32213617
  REMARK    GeneRIF: Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas
            Nnt null C57BL/6J mice do not.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3070)
  AUTHORS   X Cai,L., Tanada,Y., D Bello,G., C Fleming,J., F Alkassis,F.,
            Ladd,T., Golde,T., Koh,J., Chen,S. and Kasahara,H.
  TITLE     Cardiac MLC2 kinase is localized to the Z-disc and interacts with
            alpha-actinin2
  JOURNAL   Sci Rep 9 (1), 12580 (2019)
   PUBMED   31467300
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3070)
  AUTHORS   Choi,Y.O., Ryu,H.J., Kim,H.R., Song,Y.S., Kim,C., Lee,W., Choe,H.,
            Leem,C.H. and Jang,Y.J.
  TITLE     Implication of phosphorylation of the myosin II regulatory light
            chain in insulin-stimulated GLUT4 translocation in 3T3-F442A
            adipocytes
  JOURNAL   Exp Mol Med 38 (2), 180-189 (2006)
   PUBMED   16672772
  REMARK    GeneRIF: MLCK may be a regulatory target of Ca(2+)/calmodulin and
            may play an important role in insulin-stimulated glucose transport
            in adipocytes.
REFERENCE   7  (bases 1 to 3070)
  AUTHORS   Zhi,G., Ryder,J.W., Huang,J., Ding,P., Chen,Y., Zhao,Y., Kamm,K.E.
            and Stull,J.T.
  TITLE     Myosin light chain kinase and myosin phosphorylation effect
            frequency-dependent potentiation of skeletal muscle contraction
  JOURNAL   Proc Natl Acad Sci U S A 102 (48), 17519-17524 (2005)
   PUBMED   16299103
  REMARK    GeneRIF: Myosin light chain phosphorylation by the dedicated
            skeletal muscle Ca(2+)/calmodulin-dependent MLCK is a primary
            biochemical mechanism for tension potentiation due to repetitive
            stimulation in fast-twitch skeletal muscle.
REFERENCE   8  (bases 1 to 3070)
  AUTHORS   Fazal,F., Gu,L., Ihnatovych,I., Han,Y., Hu,W., Antic,N.,
            Carreira,F., Blomquist,J.F., Hope,T.J., Ucker,D.S. and de
            Lanerolle,P.
  TITLE     Inhibiting myosin light chain kinase induces apoptosis in vitro and
            in vivo
  JOURNAL   Mol Cell Biol 25 (14), 6259-6266 (2005)
   PUBMED   15988034
  REMARK    GeneRIF: Inhibiting MLCK increases the number of apoptotic cells
            and retards the growth of mammary cancer cells in mice.
REFERENCE   9  (bases 1 to 3070)
  AUTHORS   Deng,M., Williams,C.J. and Schultz,R.M.
  TITLE     Role of MAP kinase and myosin light chain kinase in
            chromosome-induced development of mouse egg polarity
  JOURNAL   Dev Biol 278 (2), 358-366 (2005)
   PUBMED   15680356
  REMARK    GeneRIF: MLCK is involved in egg polarity because inhibitors
            prevent sperm chromatin-induced cortical reorganization.
REFERENCE   10 (bases 1 to 3070)
  AUTHORS   Somlyo,A.V., Wang,H., Choudhury,N., Khromov,A.S., Majesky,M.,
            Owens,G.K. and Somlyo,A.P.
  TITLE     Myosin light chain kinase knockout
  JOURNAL   J Muscle Res Cell Motil 25 (3), 241-242 (2004)
   PUBMED   15467390
  REMARK    GeneRIF: Ubiquitously expressed smooth muscle MLCK activity in MLCK
            null mice may be sufficient, but is not necessary for cytokinesis
            and early morphogenesis; other Ca2+-dependent kinases can
            phosphorylate myosin light-chain (20) and induce contraction in
            embryos.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134854.3.
            
            On May 2, 2007 this sequence version replaced NM_175441.4.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146683.1, EU403565.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164140, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-636               AC134854.3         36857-37492
            637-730             AC134854.3         41608-41701
            731-1139            AC134854.3         42858-43266
            1140-1540           AC134854.3         46614-47014
            1541-1646           AC134854.3         48515-48620
            1647-1740           AC134854.3         49251-49344
            1741-1850           AC134854.3         50109-50218
            1851-1992           AC134854.3         51677-51818
            1993-2063           AC134854.3         59454-59524
            2064-2192           AC134854.3         65685-65813
            2193-2345           AC134854.3         73498-73650
            2346-2478           AC134854.3         74901-75033
            2479-3070           AC134854.3         76661-77252
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 41.61 cM"
     gene            1..3070
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /note="myosin light chain kinase 3"
                     /db_xref="GeneID:213435"
                     /db_xref="MGI:MGI:2443063"
     exon            1..636
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     CDS             151..2538
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /EC_number="2.7.11.18"
                     /note="isoform 1 is encoded by transcript variant 1;
                     putative myosin light chain kinase 3; cardiac MLCK;
                     cardiac-specific myosin light chain kinase;
                     cardiac-MyBP-C-associated Ca/CaM kinase"
                     /codon_start=1
                     /product="myosin light chain kinase 3 isoform 1"
                     /protein_id="NP_780650.2"
                     /db_xref="CCDS:CCDS40422.1"
                     /db_xref="GeneID:213435"
                     /db_xref="MGI:MGI:2443063"
                     /translation="MSGVSEEDPEGLAPQGLPALGGACLATMDKKLNVLTEKVDRLLH
                     FQEDVTEKLQCVCQGMDHLEQDLHRLEASRELSLAGSGSTPPTTAQAAWPEVLELVRA
                     VRQEGAQHGARLEALFKMVVAVDRAITLVGSTFQNSKVADFIMQGTVPGRKGSLADGP
                     EENKEQAEVAGVKPNHVLTTGGVQADASRTLWEESQKEDIPVRTVEGLPLIINTSLKG
                     ADLTQAGASLRQGVEVLGPGQVPLPTEAESRLPETASENTGATLELSVAIDRISEVLT
                     SLKMSQGGGQETSSSKPDCWLSEEAMRLSSGPLPQPLGPLTPDSDIHSGDALPRIPIN
                     MQEMATPGELLETQSGSPIGSAEAPGLGTVLEDQIPKGARPFPPLPKRSSNNGGMSAE
                     EEIGSGAEPMRGPSLATRDWRDETVGTTDLQQGIDPGAVSPEPGKDHAAQGPGRTEAG
                     RLSSAAEAAIVVLDDSAAPPAPFEHRVVSIKDTLISAGYTVSQHEVLGGGRFGQVHRC
                     TERSTGLALAAKIIKVKNVKDREDVKNEVNIMNQLSHVNLIQLYDAFESKSSFTLIME
                     YVDGGELFDRITDEKYHLTELDVVLFTRQICEGVHYLHQHYILHLDLKPENILCVSQT
                     GHQIKIIDFGLARRYKPREKLKVNFGTPEFLAPEVVNYEFVSFPTDMWSVGVITYMLL
                     SGLSPFLGETDAETMNFIVNCSWDFDADTFKGLSEEAKDFVSRLLVKEKSCRMSATQC
                     LKHEWLSHLPAKASGSNVRLRSQQLLQKYMAQSKWKKHFHVVTAVNRLRKFPTCP"
     exon            637..730
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            731..1139
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1540
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1541..1646
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1740
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1850
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1851..1992
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            1993..2063
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            2064..2192
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            2193..2345
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            2346..2478
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
     exon            2479..3070
                     /gene="Mylk3"
                     /gene_synonym="D830007F02Rik; MLCK"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 agtcaggagg gcaggctgag gctctgcagc aaggctctta ccagcggagg acaatggcct
       61 tccagatgtt agcaccaaag tgaaaggcag atccgcagat ccgtggtgct gcaggttcct
      121 tgcctttgtc cttagcaaac ccgaattggg atgtcaggag tttcagagga ggacccagag
      181 ggtctggccc cccagggtct gccagcattg ggcggagcct gcttagccac catggacaaa
      241 aaacttaatg tgctgactga gaaggtcgac agactcttgc atttccaaga agatgtcaca
      301 gagaagctgc agtgtgtgtg ccaaggcatg gatcacctgg aacaagatct gcatcggctg
      361 gaggcctccc gggagttgag tctggcaggg tctggcagca ctcccccaac cacggctcag
      421 gccgcatggc ctgaggtcct ggagctggtg agggctgtgc ggcaggaggg tgcccagcat
      481 ggtgccaggc tcgaagccct cttcaagatg gtggtggctg tggacagggc tattactttg
      541 gtagggtcca cattccagaa ttccaaggtg gctgatttca tcatgcaagg aaccgtgccc
      601 gggagaaaag gcagtctggc cgatggcccg gaggagaaca aggagcaagc agaagttgct
      661 ggagtgaagc caaaccatgt actgactaca ggaggtgtgc aagctgacgc ctctaggacg
      721 ctgtgggaag agagccaaaa ggaggacata cctgtgcgaa cagtggaggg gctgcctctc
      781 atcatcaata cgtcactgaa gggagctgac ctaacccagg caggagcctc actgaggcag
      841 ggagttgaag ttcttggccc aggccaagta cccctaccca cagaagcaga atccaggctt
      901 cctgagacag ccagtgagaa cactggagcc accctggaat tgtccgtagc aatcgacaga
      961 atcagcgagg tcctcactag cctcaagatg tcacaaggtg gtggtcaaga aacctcatcc
     1021 agcaagcctg actgttggct ttcagaagag gccatgaggc tgagttcagg gcctctccct
     1081 cagcccctag gaccactaac tccagacagt gacattcaca gtggtgatgc acttcccagg
     1141 atccctatca atatgcaaga gatggctact cctggggagt tgcttgagac ccaaagtggc
     1201 agtcccattg gctctgcaga agctccaggc cttgggactg tgttagaaga ccagatccct
     1261 aaaggagcca gaccatttcc acccctgcca aagaggagca gcaacaatgg tggcatgagt
     1321 gcagaggagg agatagggtc tggggctgag cctatgagag gaccaagctt ggctacaagg
     1381 gactggagag atgagactgt tgggaccaca gacctgcagc aaggcataga cccaggagca
     1441 gtgagccctg agcctgggaa ggaccacgca gcccagggcc caggaagaac tgaagctgga
     1501 aggctatctt ctgctgcaga ggctgccatt gtggttctag atgacagcgc agcaccccca
     1561 gccccttttg aacaccgggt agtgagcatc aaagataccc tgatctcagc aggctacacg
     1621 gtatcccaac atgaagtctt aggagggggt cggtttggcc aggtgcacag gtgtacagag
     1681 aggtctacag gccttgcact ggcagccaag atcatcaaag tgaagaacgt aaaggaccgg
     1741 gaggatgtga agaatgaggt caacatcatg aaccagctca gccacgtaaa cttgatccaa
     1801 ctttatgatg cgtttgagag caagagcagc ttcactctga tcatggagta tgtggatgga
     1861 ggcgaactct ttgaccggat cacggatgag aagtaccacc tcactgagtt ggatgtggtc
     1921 ttgttcacga ggcagatctg tgagggtgtg cattacctgc atcagcacta tatcctgcac
     1981 ctggacctca agcctgagaa catattgtgt gtcagccaga cagggcatca aattaagatc
     2041 attgactttg ggctggctag aagatacaag cctcgggaga agctaaaggt gaactttggt
     2101 actccggagt tcctggcccc agaagttgtt aactatgagt ttgtgtcatt tccaacagac
     2161 atgtggagtg tgggagttat cacctacatg ctactcagtg gtttgtcccc atttctaggg
     2221 gagacagatg cagagaccat gaattttatt gtgaactgca gctgggattt cgatgctgat
     2281 accttcaaag ggctgtcgga ggaagccaag gactttgttt cccggttact ggtcaaagag
     2341 aagagctgta ggatgagcgc cacacagtgc ctgaaacacg agtggttaag tcacctgcct
     2401 gccaaagcct cgggctccaa cgttcgcctc agatcccaac aactgctgca gaaatatatg
     2461 gctcagagta aatggaagaa acatttccac gtggtgactg cagtcaacag gctacggaaa
     2521 tttccaacgt gtccctaatc ttcaactctg ctgttccact gggcctggga attcttgagg
     2581 caacacgaag tggtaatatg aagagattac tcaagatttt acggagattg gcgctttgct
     2641 attattgatt tttcttattt tgcaaagaat gatggaagga agcaagaaag aaagaaaaga
     2701 agaaaagggg gaagaaaagg aaaaggcaga aagcaaggaa acaggctacg ttgttgctct
     2761 tcttgtaggt gaaagtgttt ttattaaaag ccctaggaat gtttttctgc ctcgtaaggt
     2821 cagcaggtct catatgctgc ttgctccccc gcacccttcc ttttggtaat aagagcaggc
     2881 acgctcagga tgggcaggga aatcctactt ggcttttggt caaatttgaa ttctaaactt
     2941 gtcatgatta aagaagccag tagggagtga ggtatggaag agggaggaat taggtccaac
     3001 agtgggggat gaatttgacg gaaacattgt ataaaattct taaagaatta ataaaatata
     3061 tttttaaagg
//
