LOCUS       NP_001074513             613 aa            linear   ROD 01-JUN-2024
DEFINITION  myosin light chain kinase 2, skeletal/cardiac muscle [Mus
            musculus].
ACCESSION   NP_001074513 XP_001005681
VERSION     NP_001074513.2
DBSOURCE    REFSEQ: accession NM_001081044.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 613)
  AUTHORS   Al Madhoun,A.S., Mehta,V., Li,G., Figeys,D., Wiper-Bergeron,N. and
            Skerjanc,I.S.
  TITLE     Skeletal myosin light chain kinase regulates skeletal myogenesis by
            phosphorylation of MEF2C
  JOURNAL   EMBO J 30 (12), 2477-2489 (2011)
   PUBMED   21556048
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 613)
  AUTHORS   Khiem,D., Cyster,J.G., Schwarz,J.J. and Black,B.L.
  TITLE     A p38 MAPK-MEF2C pathway regulates B-cell proliferation
  JOURNAL   Proc Natl Acad Sci U S A 105 (44), 17067-17072 (2008)
   PUBMED   18955699
REFERENCE   3  (residues 1 to 613)
  AUTHORS   Moitra,J., Evenoski,C., Sammani,S., Wadgaonkar,R., Turner,J.R.,
            Ma,S.F. and Garcia,J.G.
  TITLE     A transgenic mouse with vascular endothelial over-expression of the
            non-muscle myosin light chain kinase-2 isoform is susceptible to
            inflammatory lung injury: role of sexual dimorphism and age
  JOURNAL   Transl Res 151 (3), 141-153 (2008)
   PUBMED   18279813
  REMARK    GeneRIF: These data reveal that nmMLCK2 overexpression in the
            endothelium exacerbates lung injury in vivo in a sexually dimorphic
            and age-dependent manner.
REFERENCE   4  (residues 1 to 613)
  AUTHORS   Jevsek,M., Jaworski,A., Polo-Parada,L., Kim,N., Fan,J.,
            Landmesser,L.T. and Burden,S.J.
  TITLE     CD24 is expressed by myofiber synaptic nuclei and regulates
            synaptic transmission
  JOURNAL   Proc Natl Acad Sci U S A 103 (16), 6374-6379 (2006)
   PUBMED   16606832
REFERENCE   5  (residues 1 to 613)
  AUTHORS   Zhi,G., Ryder,J.W., Huang,J., Ding,P., Chen,Y., Zhao,Y., Kamm,K.E.
            and Stull,J.T.
  TITLE     Myosin light chain kinase and myosin phosphorylation effect
            frequency-dependent potentiation of skeletal muscle contraction
  JOURNAL   Proc Natl Acad Sci U S A 102 (48), 17519-17524 (2005)
   PUBMED   16299103
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL833801.9.
            
            On Dec 20, 2007 this sequence version replaced NP_001074513.1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC158041.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..613
                     /organism="Mus musculus"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 75.41 cM"
     Protein         1..613
                     /product="myosin light chain kinase 2, skeletal/cardiac
                     muscle"
                     /EC_number="2.7.11.18"
                     /note="MLCK2"
                     /calculated_mol_wt=65859
     Region          1..168
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8VCR8.2)"
     Site            161
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8VCR8.2)"
     Site            167
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8VCR8.2)"
     Site            169
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q8VCR8.2)"
     Region          219..240
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8VCR8.2)"
     Region          298..557
                     /region_name="STKc_MLCK2"
                     /note="Catalytic domain of the Serine/Threonine Kinase,
                     Myosin Light Chain Kinase 2; cd14190"
                     /db_xref="CDD:271092"
     Site            order(308..312,314,316,329,331,360,376..377,379,383,385,
                     423,425,427..428,430,442..443,446,461..464)
                     /site_type="active"
                     /db_xref="CDD:271092"
     Site            order(308..312,314,316,329,331,360,376..377,379,383,
                     427..428,430,442..443)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:271092"
     Site            order(312,383,385,423,425,427,446,461..464)
                     /site_type="other"
                     /note="polypeptide substrate binding site [polypeptide
                     binding]"
                     /db_xref="CDD:271092"
     Site            442..464
                     /site_type="other"
                     /note="activation loop (A-loop)"
                     /db_xref="CDD:271092"
     Site            462
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P20689; propagated from
                     UniProtKB/Swiss-Prot (Q8VCR8.2)"
     Region          591..603
                     /region_name="Calmodulin-binding. /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q8VCR8.2)"
     CDS             1..613
                     /gene="Mylk2"
                     /gene_synonym="9830004H17Rik"
                     /coded_by="NM_001081044.2:197..2038"
                     /db_xref="CCDS:CCDS38282.2"
                     /db_xref="GeneID:228785"
                     /db_xref="MGI:MGI:2139434"
ORIGIN      
        1 mttengavel gsqslsteqt pkaaagdgps asekepsapa tekdlsppna kkdpgapdpk
       61 nnpdppslkk dpakapgpek kgdpvpasas sqgpsgegdg gggpaegseg ppaalplpta
      121 taeasiqkld ptqapsgnqg sgeakagkka aecreagrrg spaflhspsc paiiscsekt
      181 lavkplsett dlvftgvsvt pdpqdpgpvk aggtnalaek kkeeaekasg qagqakvqgd
      241 tpqrigfqav pservevgqa lcltareedc fqilddcppp papfphrive lrtgnvnsef
      301 smnskealgg gkfgavctct eratglklaa kvikkqtpkd kemvlleiev mnqlnhrnli
      361 qlyaaietsh eiilfmeyie ggelferivd edyhltevdt mvfvrqicdg ilfmhkmrvl
      421 hldlkpenil cvnttghlvk iidfglarry npneklkvnf gtpeflspev vnydqisdkt
      481 dmwslgvity mllsglspfl gdddtetlnn vlsanwyfde etfeavsdea kdfvsnlltk
      541 dqsarmsaeq clahpwlnnl aekakrcnrr lksqillkky lmkrrwkknf iavsaanrfk
      601 kisssgalma lgv
//
