LOCUS       NM_153789               2983 bp    mRNA    linear   ROD 02-JUL-2024
DEFINITION  Mus musculus myosin regulatory light chain interacting protein
            (Mylip), mRNA.
ACCESSION   NM_153789 NM_181043 XM_978411
VERSION     NM_153789.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2983)
  AUTHORS   Gao,J., Littman,R., Diamante,G., Xiao,X., Ahn,I.S., Yang,X.,
            Cole,T.A. and Tontonoz,P.
  TITLE     Therapeutic IDOL Reduction Ameliorates Amyloidosis and Improves
            Cognitive Function in APP/PS1 Mice
  JOURNAL   Mol Cell Biol 40 (8), e00518-19 (2020)
   PUBMED   31964754
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2983)
  AUTHORS   Lee,S.D., Priest,C., Bjursell,M., Gao,J., Arneson,D.V., Ahn,I.S.,
            Diamante,G., van Veen,J.E., Massa,M.G., Calkin,A.C., Kim,J.,
            Andersen,H., Rajbhandari,P., Porritt,M., Carreras,A., Ahnmark,A.,
            Seeliger,F., Maxvall,I., Eliasson,P., Althage,M., Akerblad,P.,
            Linden,D., Cole,T.A., Lee,R., Boyd,H., Bohlooly-Y,M., Correa,S.M.,
            Yang,X., Tontonoz,P. and Hong,C.
  TITLE     IDOL regulates systemic energy balance through control of neuronal
            VLDLR expression
  JOURNAL   Nat Metab 1 (11), 1089-1100 (2019)
   PUBMED   32072135
REFERENCE   5  (bases 1 to 2983)
  AUTHORS   van Loon,N.M., Ottenhoff,R., Kooijman,S., Moeton,M., Scheij,S.,
            Roscam Abbing,R.L.P., Gijbels,M.J.J., Levels,J.H.M., Sorrentino,V.,
            Berbee,J.F.P., Rensen,P.C.N. and Zelcer,N.
  TITLE     Inactivation of the E3 Ubiquitin Ligase IDOL Attenuates
            Diet-Induced Obesity and Metabolic Dysfunction in Mice
  JOURNAL   Arterioscler Thromb Vasc Biol 38 (8), 1785-1795 (2018)
   PUBMED   29903737
  REMARK    GeneRIF: Hepatic Idol expression has a role in the development of
            diet induced obesity and metabolic syndrome in mice.
REFERENCE   6  (bases 1 to 2983)
  AUTHORS   Scotti,E., Hong,C., Yoshinaga,Y., Tu,Y., Hu,Y., Zelcer,N.,
            Boyadjian,R., de Jong,P.J., Young,S.G., Fong,L.G. and Tontonoz,P.
  TITLE     Targeted disruption of the idol gene alters cellular regulation of
            the low-density lipoprotein receptor by sterols and liver x
            receptor agonists
  JOURNAL   Mol Cell Biol 31 (9), 1885-1893 (2011)
   PUBMED   21343340
REFERENCE   7  (bases 1 to 2983)
  AUTHORS   Ishibashi,M., Masson,D., Westerterp,M., Wang,N., Sayers,S., Li,R.,
            Welch,C.L. and Tall,A.R.
  TITLE     Reduced VLDL clearance in Apoe(-/-)Npc1(-/-) mice is associated
            with increased Pcsk9 and Idol expression and decreased hepatic
            LDL-receptor levels
  JOURNAL   J Lipid Res 51 (9), 2655-2663 (2010)
   PUBMED   20562239
  REMARK    GeneRIF: Data show that NPC1 deficiency has a major impact on VLDL
            metabolism in Apoe(-/-) mice through modulation of hepatic LDL-R
            protein levels, and a modest one on Pcsk9 and Idol induction.
REFERENCE   8  (bases 1 to 2983)
  AUTHORS   Hong,C., Duit,S., Jalonen,P., Out,R., Scheer,L., Sorrentino,V.,
            Boyadjian,R., Rodenburg,K.W., Foley,E., Korhonen,L., Lindholm,D.,
            Nimpf,J., van Berkel,T.J., Tontonoz,P. and Zelcer,N.
  TITLE     The E3 ubiquitin ligase IDOL induces the degradation of the low
            density lipoprotein receptor family members VLDLR and ApoER2
  JOURNAL   J Biol Chem 285 (26), 19720-19726 (2010)
   PUBMED   20427281
  REMARK    GeneRIF: Data show that the identification of VLDLR and ApoER2 as
            Idol(Mylip) targets suggests potential roles for this LXR-inducible
            E3 ligase in the central nervous system in addition to lipid
            metabolism.
REFERENCE   9  (bases 1 to 2983)
  AUTHORS   Zelcer,N., Hong,C., Boyadjian,R. and Tontonoz,P.
  TITLE     LXR regulates cholesterol uptake through Idol-dependent
            ubiquitination of the LDL receptor
  JOURNAL   Science 325 (5936), 100-104 (2009)
   PUBMED   19520913
  REMARK    GeneRIF: study shows the LXR-Idol(Mylip)-LDLR axis defines a
            complementary pathway to sterol response element-binding proteins
            for sterol regulation of cholesterol uptake
REFERENCE   10 (bases 1 to 2983)
  AUTHORS   Knowlton,M.N., Chan,B.M. and Kelly,G.M.
  TITLE     The zebrafish band 4.1 member Mir is involved in cell movements
            associated with gastrulation
  JOURNAL   Dev Biol 264 (2), 407-429 (2003)
   PUBMED   14651927
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC154521.2.
            
            On or before Jun 15, 2012 this sequence version replaced
            NM_181043.1, NM_153789.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK034887.1, AK159478.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-302               AC154521.2         133502-133803       c
            303-493             AC154521.2         132166-132356       c
            494-679             AC154521.2         118960-119145       c
            680-877             AC154521.2         117455-117652       c
            878-1042            AC154521.2         116824-116988       c
            1043-1463           AC154521.2         114769-115189       c
            1464-2983           AC154521.2         111605-113124       c
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 21.88 cM"
     gene            1..2983
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /note="myosin regulatory light chain interacting protein"
                     /db_xref="GeneID:218203"
                     /db_xref="MGI:MGI:2388271"
     exon            1..302
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /inference="alignment:Splign:2.1.0"
     CDS             216..1553
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /EC_number="2.3.2.27"
                     /note="idol; inducible degrader of the LDL-receptor;
                     RING-type E3 ubiquitin transferase MYLIP"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase MYLIP"
                     /protein_id="NP_722484.2"
                     /db_xref="CCDS:CCDS36648.1"
                     /db_xref="GeneID:218203"
                     /db_xref="MGI:MGI:2388271"
                     /translation="MLCYVTRPDAVLMEVEVEAKANGEDCLNQVCRRLGIIEVDYFGL
                     QFTGSKGESLWLNLRNRISQQMDGLAPYRLKLRVKFFVEPHLILQEQTRHIFFLHIKE
                     SLLAGHLQCSPEQAVELSALLAQTKFGDYNQNTAQYSYEDLCEKELSSSTLNSIVAKH
                     KELEGISQASAEYQVLQIVSAMENYGIEWHAVRDSEGQKLLIGVGPEGISICKEDFSP
                     INRIAYPVVQMATQSGKNVYLTVTKESGNSIVLLFKMISTRAASGLYRAITETHAFYR
                     CDTVTSAVMMQYSRDLKGHLASLFLNENINLGKKYVFDIKRTSKEVYDHARRALYNAG
                     VVDLVSRSDQSPPSSPLKSSDSSMSCSSCEGLSCQQTRVLQEKLRKLKEAMLCMACCE
                     EEINSTFCPCGHTVCCESCAAQLQSCPVCRSRVEHVQHVYLPTHTSLLNLTVI"
     exon            303..493
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            494..679
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            680..877
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            878..1042
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1463
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..2983
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2890..2895
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /note="hexamer: ATTAAA"
     polyA_site      2914
                     /gene="Mylip"
                     /gene_synonym="9430057C20Rik; Mir"
                     /note="major polyA site"
ORIGIN      
        1 agtagggctg tcggagcggc gcggccgtgt agctcccggg aactggctgt cgtgggggtg
       61 gcggggacgc gagtggcggc tgcgtggggt gcagggcggg tggccgcacg gctgcacctt
      121 cctcacggag cccggagtcg acttggagca attgcggtga ggcgacagct ccggcgcaca
      181 cccgagaaga agcggcggtg gcggcggccc cagccatgct gtgctatgtg acgaggccgg
      241 acgcggtgct gatggaggta gaggtggagg caaaagccaa cggcgaggac tgtctcaacc
      301 aggtgtgcag gcgtctaggg atcatcgagg ttgattattt tgggctgcag ttcacgggga
      361 gcaaaggtga gagcttatgg ctgaatctga gaaaccggat ctcccagcag atggatgggc
      421 tggcacctta ccgccttaaa ctgagggtca agttctttgt ggagcctcat ctcatcttac
      481 aggagcagac aaggcatatc tttttcttgc acattaaaga gtccctcttg gcaggccacc
      541 tccagtgttc cccagagcag gccgtggaac tcagtgccct cctggctcag accaaatttg
      601 gagactacaa ccagaacacc gcccaataca gctatgagga cctgtgtgag aaagagctct
      661 ccagctccac tttgaacagc atcgttgcga agcataagga gctggagggc atcagccagg
      721 cctctgccga gtaccaggtt ctgcagattg tgtcagcgat ggagaactac ggcatagagt
      781 ggcatgctgt gagggacagc gaaggacaga aactcctcat tggggtcgga cctgaaggca
      841 tctcgatctg taaagaggac tttagcccta ttaacaggat agcttatcct gtggtgcaga
      901 tggccaccca gtcaggaaag aatgtctact tgaccgtcac caaggagtcc ggcaacagca
      961 tcgtgctcct gtttaagatg atcagcacca gagcagccag cggcctctac cgagccatca
     1021 ccgaaacaca tgcattctat aggtgtgaca cagtcaccag tgccgtcatg atgcagtaca
     1081 gtcgcgacct gaagggccac ttggcgtctc tgtttctgaa cgaaaacatt aaccttggta
     1141 agaaatacgt cttcgacatc aagagaacat ccaaagaggt ctatgaccat gccaggaggg
     1201 ctctgtacaa cgccggcgtt gtggaccttg tctctcgaag tgaccagagc ccccccagct
     1261 cacccctgaa gtcctcagac agcagcatga gctgcagcag ctgtgagggc ctcagctgcc
     1321 agcagacccg ggtgctgcag gagaagctgc gcaagctgaa ggaagccatg ctgtgtatgg
     1381 cgtgctgcga ggaggagatc aactccacct tctgcccctg cggccacact gtgtgctgcg
     1441 agagctgtgc agcccagctg cagtcctgtc cggtctgcag atcccgtgtg gagcatgtcc
     1501 agcacgtcta cctgcccacc cacaccagtc tcctcaatct gactgtcatc tgatgcgtcc
     1561 tgcactcgat ggacaagcca tgtccccaca agctgcagta ttgtaaacta taagaataat
     1621 aactttgtga agagctattt cactctcaac acccatctgc catgagacat tttcagaaac
     1681 aaggaggaaa agaaaacaag aatgtgacca cacctcttcc gtgaggagaa gcaacaggcc
     1741 ccatggccac caggaagaac tctgggacac ggacacattc cttgaacttt agggttggtt
     1801 ttttttttta atgatcaagt aaaggagtag atagaatcgt cttcgtcagt caagtggcaa
     1861 catggcccaa ccgtgggcac cttttaggaa atgacgtcat atgtctcctt cactttttcc
     1921 ccgggcagca gattttgtaa gtgttttaag gatttccttg gttctttttg tatggtcatg
     1981 gagcgctgaa tatttttaat agggattttt tttcttaaag aaatagtcct cattataaaa
     2041 gtcatttctg tctttataac tcattcaaga acaactggaa aagctggcag attgaaaaaa
     2101 aaaaagcaat cctgtgactt cccaagggtt gacagcaatg ttgtcagatt ggaagcagtc
     2161 tggctgagag ccaataggta actcaccgtg ggtgacttcc ttcctagagc ccttccgttt
     2221 cccctcattc cacaccccat gcctttcact gataaaaatg ctaccagttt ggttaagaga
     2281 catacatggt agagtcaagc actccctggg ctttggagat tggaaagcga gagtagcttc
     2341 cttgaaggaa aaagatgaga gagagagaga gagagagaga gagagagaga gagagagaga
     2401 gagagagaga tgagccagag agccactcag tatgcccgag tggttcttca ctttcccaag
     2461 cactcactcc agctgcaccc atgggtgtcg ccttgcttga agatcaaact ttctacagcc
     2521 ttataggttt ctagatagtg tctccttttt gtgtatgtct tgtttctcgt tgttcgagtt
     2581 ttcctatgtc agtgcttcca tactcattgt cctgccccct cggtgtcttc cagaggtagg
     2641 gctacttctt tatgtttcca tattctaagt tttcaccccc acttgggcat tttggaagct
     2701 agtgagctag ggggttttct agggtgtcag gaaacctagc tgacctcatc gggtgcaata
     2761 ctagctaagt taaagctaga agcctacact gtcactttac tgagatttct gagtctacgt
     2821 ttcatattgc cttaatgtag cagtaatgtg tttatgcatt tgtttctttg cacagacatt
     2881 ttgtcagata ttaaaactct acttttttat ggcacatatt agcatataag cctttattcc
     2941 aagaggtatt tattttttca cttgtaaaaa aaataatgtt tcc
//
