LOCUS       NM_010861                677 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus myosin, light polypeptide 2, regulatory, cardiac, slow
            (Myl2), transcript variant 1, mRNA.
ACCESSION   NM_010861
VERSION     NM_010861.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Argiro,L., Chevalier,C., Choquet,C., Nandkishore,N., Ghata,A.,
            Baudot,A., Zaffran,S. and Lescroart,F.
  TITLE     Gastruloids are competent to specify both cardiac and skeletal
            muscle lineages
  JOURNAL   Nat Commun 15 (1), 10172 (2024)
   PUBMED   39580459
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  AUTHORS   Allwood,M.A., Edgett,B.A., Platt,M.J., Marrow,J.P., Coyle-Asbil,B.,
            Holjak,E.J.B., Nelson,V.L., Bangali,S., Alshamali,R., Jacyniak,K.,
            Klein,J.M., Farquharson,L., Romanova,N., Northrup,V., Ogilvie,L.M.,
            Ayoub,A., Ask,K., Vickaryous,M.K., Hare,G.M.T., Brunt,K.R. and
            Simpson,J.A.
  TITLE     Novel roles of cardiac-derived erythropoietin in cardiac
            development and function
  JOURNAL   J Mol Cell Cardiol 188, 90-104 (2024)
   PUBMED   38382296
REFERENCE   3  (bases 1 to 677)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 677)
  AUTHORS   Villeneuve,C., Hashmi,A., Ylivinkka,I., Lawson-Keister,E.,
            Miroshnikova,Y.A., Perez-Gonzalez,C., Myllymaki,S.M., Bertillot,F.,
            Yadav,B., Zhang,T., Matic Vignjevic,D., Mikkola,M.L., Manning,M.L.
            and Wickstrom,S.A.
  TITLE     Mechanical forces across compartments coordinate cell shape and
            fate transitions to generate tissue architecture
  JOURNAL   Nat Cell Biol 26 (2), 207-218 (2024)
   PUBMED   38302719
REFERENCE   5  (bases 1 to 677)
  AUTHORS   Sheikh,F., Lyon,R.C. and Chen,J.
  TITLE     Functions of myosin light chain-2 (MYL2) in cardiac muscle and
            disease
  JOURNAL   Gene 569 (1), 14-20 (2015)
   PUBMED   26074085
  REMARK    Review article
            Erratum:[Gene. 2015 Oct 15;571(1):151]
REFERENCE   6  (bases 1 to 677)
  AUTHORS   Lee,K.F., Simon,H., Chen,H., Bates,B., Hung,M.C. and Hauser,C.
  TITLE     Requirement for neuregulin receptor erbB2 in neural and cardiac
            development
  JOURNAL   Nature 378 (6555), 394-398 (1995)
   PUBMED   7477377
REFERENCE   7  (bases 1 to 677)
  AUTHORS   Lyons,I., Parsons,L.M., Hartley,L., Li,R., Andrews,J.E., Robb,L.
            and Harvey,R.P.
  TITLE     Myogenic and morphogenetic defects in the heart tubes of murine
            embryos lacking the homeo box gene Nkx2-5
  JOURNAL   Genes Dev 9 (13), 1654-1666 (1995)
   PUBMED   7628699
REFERENCE   8  (bases 1 to 677)
  AUTHORS   Lee,K.J., Ross,R.S., Rockman,H.A., Harris,A.N., O'Brien,T.X., van
            Bilsen,M., Shubeita,H.E., Kandolf,R., Brem,G., Price,J. et al.
  TITLE     Myosin light chain-2 luciferase transgenic mice reveal distinct
            regulatory programs for cardiac and skeletal muscle-specific
            expression of a single contractile protein gene
  JOURNAL   J Biol Chem 267 (22), 15875-15885 (1992)
   PUBMED   1379240
REFERENCE   9  (bases 1 to 677)
  AUTHORS   Hastings,K.E. and Emerson,C.P. Jr.
  TITLE     cDNA clone analysis of six co-regulated mRNAs encoding skeletal
            muscle contractile proteins
  JOURNAL   Proc Natl Acad Sci U S A 79 (5), 1553-1557 (1982)
   PUBMED   6951196
REFERENCE   10 (bases 1 to 677)
  AUTHORS   Czosnek,H., Nudel,U., Shani,M., Barker,P.E., Pravtcheva,D.D.,
            Ruddle,F.H. and Yaffe,D.
  TITLE     The genes coding for the muscle contractile proteins, myosin heavy
            chain, myosin light chain 2, and skeletal muscle actin are located
            on three different mouse chromosomes
  JOURNAL   EMBO J 1 (11), 1299-1305 (1982)
   PUBMED   6897916
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140353.2, AK002367.1 and BX529018.1.
            
            On Jan 11, 2017 this sequence version replaced NM_010861.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC061144.1, AK002367.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-29                AC140353.2         120269-120297
            30-661              AK002367.1         2-633
            662-677             BX529018.1         411-426
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5"
     gene            1..677
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="myosin, light polypeptide 2, regulatory, cardiac,
                     slow"
                     /db_xref="GeneID:17906"
                     /db_xref="MGI:MGI:97272"
     exon            1..79
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..577
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="isoform 1 is encoded by transcript variant 1;
                     myosin light chain 2v; myosin light chain,
                     phosphorylatable, cardiac ventricles"
                     /codon_start=1
                     /product="myosin regulatory light chain 2,
                     ventricular/cardiac muscle isoform isoform 1"
                     /protein_id="NP_034991.3"
                     /db_xref="CCDS:CCDS39252.1"
                     /db_xref="GeneID:17906"
                     /db_xref="MGI:MGI:97272"
                     /translation="MAPKKAKKRIEGGSSNVFSMFEQTQIQEFKEAFTIMDQNRDGFI
                     DKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNA
                     FKVFDPEGKGSLKADYVREMLTTQAERFSKEEIDQMFAAFPPDVTGNLDYKNLVHIIT
                     HGEEKD"
     exon            80..169
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            170..245
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            246..350
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            351..429
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            430..478
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            479..662
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      637..642
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="hexamer: AATAAA"
     polyA_site      662
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="major polyA site"
ORIGIN      
        1 tttattgttc cacagcaggg gcagaggcca gcaggctcct cgaactctcc agaggtggca
       61 actggcctca gacaccatgg caccaaagaa agccaagaag cggatagaag gcgggagctc
      121 caacgtgttc tccatgtttg agcagaccca gatccaggag ttcaaggaag ccttcacaat
      181 catggaccag aacagagacg gcttcatcga caagaatgac ctaagggaca catttgctgc
      241 cctaggacga gtgaacgtga aaaatgaaga gatcgatgaa atgatcaaag aggctccagg
      301 tccaattaac ttcaccgtgt tcctcacgat gtttggggag aaacttaaag gggctgatcc
      361 tgaagagacc attctcaacg cattcaaggt gtttgatccc gagggcaaag ggtcactgaa
      421 ggctgactat gtccgggaga tgctgaccac acaagcagag aggttctcca aagaggagat
      481 cgaccagatg ttcgcagcct ttccccctga cgtcaccggc aatcttgatt ataagaattt
      541 ggtccacatc attacccacg gagaagagaa ggactgagcc ctgaaccaca gcctcaggtg
      601 acccacagcc cactctccat cccagggctg tgcgcaaata aacaggaagt cttggctctg
      661 gcaaaaaaaa aaaaaaa
//
