LOCUS       NM_001384329            1421 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus myosin, light polypeptide 2, regulatory, cardiac, slow
            (Myl2), transcript variant 5, mRNA.
ACCESSION   NM_001384329
VERSION     NM_001384329.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Argiro,L., Chevalier,C., Choquet,C., Nandkishore,N., Ghata,A.,
            Baudot,A., Zaffran,S. and Lescroart,F.
  TITLE     Gastruloids are competent to specify both cardiac and skeletal
            muscle lineages
  JOURNAL   Nat Commun 15 (1), 10172 (2024)
   PUBMED   39580459
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  AUTHORS   Allwood,M.A., Edgett,B.A., Platt,M.J., Marrow,J.P., Coyle-Asbil,B.,
            Holjak,E.J.B., Nelson,V.L., Bangali,S., Alshamali,R., Jacyniak,K.,
            Klein,J.M., Farquharson,L., Romanova,N., Northrup,V., Ogilvie,L.M.,
            Ayoub,A., Ask,K., Vickaryous,M.K., Hare,G.M.T., Brunt,K.R. and
            Simpson,J.A.
  TITLE     Novel roles of cardiac-derived erythropoietin in cardiac
            development and function
  JOURNAL   J Mol Cell Cardiol 188, 90-104 (2024)
   PUBMED   38382296
REFERENCE   3  (bases 1 to 1421)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 1421)
  AUTHORS   Villeneuve,C., Hashmi,A., Ylivinkka,I., Lawson-Keister,E.,
            Miroshnikova,Y.A., Perez-Gonzalez,C., Myllymaki,S.M., Bertillot,F.,
            Yadav,B., Zhang,T., Matic Vignjevic,D., Mikkola,M.L., Manning,M.L.
            and Wickstrom,S.A.
  TITLE     Mechanical forces across compartments coordinate cell shape and
            fate transitions to generate tissue architecture
  JOURNAL   Nat Cell Biol 26 (2), 207-218 (2024)
   PUBMED   38302719
REFERENCE   5  (bases 1 to 1421)
  AUTHORS   Sheikh,F., Lyon,R.C. and Chen,J.
  TITLE     Functions of myosin light chain-2 (MYL2) in cardiac muscle and
            disease
  JOURNAL   Gene 569 (1), 14-20 (2015)
   PUBMED   26074085
  REMARK    Review article
            Erratum:[Gene. 2015 Oct 15;571(1):151]
REFERENCE   6  (bases 1 to 1421)
  AUTHORS   Lee,K.F., Simon,H., Chen,H., Bates,B., Hung,M.C. and Hauser,C.
  TITLE     Requirement for neuregulin receptor erbB2 in neural and cardiac
            development
  JOURNAL   Nature 378 (6555), 394-398 (1995)
   PUBMED   7477377
REFERENCE   7  (bases 1 to 1421)
  AUTHORS   Lyons,I., Parsons,L.M., Hartley,L., Li,R., Andrews,J.E., Robb,L.
            and Harvey,R.P.
  TITLE     Myogenic and morphogenetic defects in the heart tubes of murine
            embryos lacking the homeo box gene Nkx2-5
  JOURNAL   Genes Dev 9 (13), 1654-1666 (1995)
   PUBMED   7628699
REFERENCE   8  (bases 1 to 1421)
  AUTHORS   Lee,K.J., Ross,R.S., Rockman,H.A., Harris,A.N., O'Brien,T.X., van
            Bilsen,M., Shubeita,H.E., Kandolf,R., Brem,G., Price,J. et al.
  TITLE     Myosin light chain-2 luciferase transgenic mice reveal distinct
            regulatory programs for cardiac and skeletal muscle-specific
            expression of a single contractile protein gene
  JOURNAL   J Biol Chem 267 (22), 15875-15885 (1992)
   PUBMED   1379240
REFERENCE   9  (bases 1 to 1421)
  AUTHORS   Hastings,K.E. and Emerson,C.P. Jr.
  TITLE     cDNA clone analysis of six co-regulated mRNAs encoding skeletal
            muscle contractile proteins
  JOURNAL   Proc Natl Acad Sci U S A 79 (5), 1553-1557 (1982)
   PUBMED   6951196
REFERENCE   10 (bases 1 to 1421)
  AUTHORS   Czosnek,H., Nudel,U., Shani,M., Barker,P.E., Pravtcheva,D.D.,
            Ruddle,F.H. and Yaffe,D.
  TITLE     The genes coding for the muscle contractile proteins, myosin heavy
            chain, myosin light chain 2, and skeletal muscle actin are located
            on three different mouse chromosomes
  JOURNAL   EMBO J 1 (11), 1299-1305 (1982)
   PUBMED   6897916
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140353.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR5189679.82325.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849390 [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-507               AC140353.2         112507-113013
            508-530             AC140353.2         113146-113168
            531-605             AC140353.2         113709-113783
            606-717             AC140353.2         113965-114076
            718-838             AC140353.2         115986-116106
            839-928             AC140353.2         121067-121156
            929-1004            AC140353.2         122014-122089
            1005-1109           AC140353.2         123149-123253
            1110-1188           AC140353.2         124145-124223
            1189-1237           AC140353.2         124306-124354
            1238-1421           AC140353.2         125989-126172
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5"
     gene            1..1421
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="myosin, light polypeptide 2, regulatory, cardiac,
                     slow"
                     /db_xref="GeneID:17906"
                     /db_xref="MGI:MGI:97272"
     exon            1..507
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            508..530
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            531..605
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            606..717
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            718..838
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            839..928
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     CDS             893..1336
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="isoform 2 is encoded by transcript variant 5;
                     myosin light chain 2v; myosin light chain,
                     phosphorylatable, cardiac ventricles"
                     /codon_start=1
                     /product="myosin regulatory light chain 2,
                     ventricular/cardiac muscle isoform isoform 2"
                     /protein_id="NP_001371258.1"
                     /db_xref="GeneID:17906"
                     /db_xref="MGI:MGI:97272"
                     /translation="MFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKN
                     EEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGSLKADYVRE
                     MLTTQAERFSKEEIDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD"
     exon            929..1004
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            1005..1109
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1188
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            1189..1237
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            1238..1421
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1396..1401
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="hexamer: AATAAA"
     polyA_site      1421
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="major polyA site"
ORIGIN      
        1 agccagccag gcagcctggg ctgaaaatgc aattggggga aaaagagacc aacagtgtct
       61 ggctcctggg ggcggggtgt gtgggggtgt ctgctccaga gtgggggaca gtggggactg
      121 ctggccaaaa ggaagtacac agagaggaac tccaacaacc tgaaccaaat ccagtgttgc
      181 ccaagcagag agtctggttg tggggccgac agaaaggatc atctctgaca ctcagttttg
      241 ttatctgaga aatgggcctt attatataca catctgctta cggtctggtt tgggatggca
      301 cagaccggga ggagtttggg cagtgcccgg catgccctgg gaggcgggca agccggtgac
      361 atgcacttgg tgtttctggg gcctcagtga aggaagatcg ctgcagaggc tggggatgtc
      421 gctgtgtgaa gccctcttcc acttctgagg acccagcaca aaccaaacgg acaagaccat
      481 ttgaactcgg agcatctgcc cttaagagcc acccgcagtc acagcacaca gtctagcctg
      541 cgctgggtgt aagagctgac ctgactgaac acagctgagc tgaggagcct tccagaatgt
      601 tcaaggagga gatacgttat ccttggtgac agtagccatc agccacactc tagaatggca
      661 gcgacttcta cagagacatg gactccatca cccggcacgc aaggcagact ggtcatgtct
      721 tggatggatg ctggaaccat cggatcctgt aacccgcttc tccacctcca gtacagacgt
      781 caggacttga ggctccctcc gtctgctctc ttctgcactg cacttgaatt tccctgaggc
      841 accaaagaaa gccaagaagc ggatagaagg cgggagctcc aacgtgttct ccatgtttga
      901 gcagacccag atccaggagt tcaaggaagc cttcacaatc atggaccaga acagagacgg
      961 cttcatcgac aagaatgacc taagggacac atttgctgcc ctaggacgag tgaacgtgaa
     1021 aaatgaagag atcgatgaaa tgatcaaaga ggctccaggt ccaattaact tcaccgtgtt
     1081 cctcacgatg tttggggaga aacttaaagg ggctgatcct gaagagacca ttctcaacgc
     1141 attcaaggtg tttgatcccg agggcaaagg gtcactgaag gctgactatg tccgggagat
     1201 gctgaccaca caagcagaga ggttctccaa agaggagatc gaccagatgt tcgcagcctt
     1261 tccccctgac gtcaccggca atcttgatta taagaatttg gtccacatca ttacccacgg
     1321 agaagagaag gactgagccc tgaaccacag cctcaggtga cccacagccc actctccatc
     1381 ccagggctgt gcgcaaataa acaggaagtc ttggctctgg c
//
