LOCUS       NM_001384326             893 bp    mRNA    linear   ROD 25-FEB-2025
DEFINITION  Mus musculus myosin, light polypeptide 2, regulatory, cardiac, slow
            (Myl2), transcript variant 2, mRNA.
ACCESSION   NM_001384326 XM_006530186
VERSION     NM_001384326.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Argiro,L., Chevalier,C., Choquet,C., Nandkishore,N., Ghata,A.,
            Baudot,A., Zaffran,S. and Lescroart,F.
  TITLE     Gastruloids are competent to specify both cardiac and skeletal
            muscle lineages
  JOURNAL   Nat Commun 15 (1), 10172 (2024)
   PUBMED   39580459
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 893)
  AUTHORS   Allwood,M.A., Edgett,B.A., Platt,M.J., Marrow,J.P., Coyle-Asbil,B.,
            Holjak,E.J.B., Nelson,V.L., Bangali,S., Alshamali,R., Jacyniak,K.,
            Klein,J.M., Farquharson,L., Romanova,N., Northrup,V., Ogilvie,L.M.,
            Ayoub,A., Ask,K., Vickaryous,M.K., Hare,G.M.T., Brunt,K.R. and
            Simpson,J.A.
  TITLE     Novel roles of cardiac-derived erythropoietin in cardiac
            development and function
  JOURNAL   J Mol Cell Cardiol 188, 90-104 (2024)
   PUBMED   38382296
REFERENCE   3  (bases 1 to 893)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 893)
  AUTHORS   Villeneuve,C., Hashmi,A., Ylivinkka,I., Lawson-Keister,E.,
            Miroshnikova,Y.A., Perez-Gonzalez,C., Myllymaki,S.M., Bertillot,F.,
            Yadav,B., Zhang,T., Matic Vignjevic,D., Mikkola,M.L., Manning,M.L.
            and Wickstrom,S.A.
  TITLE     Mechanical forces across compartments coordinate cell shape and
            fate transitions to generate tissue architecture
  JOURNAL   Nat Cell Biol 26 (2), 207-218 (2024)
   PUBMED   38302719
REFERENCE   5  (bases 1 to 893)
  AUTHORS   Sheikh,F., Lyon,R.C. and Chen,J.
  TITLE     Functions of myosin light chain-2 (MYL2) in cardiac muscle and
            disease
  JOURNAL   Gene 569 (1), 14-20 (2015)
   PUBMED   26074085
  REMARK    Review article
            Erratum:[Gene. 2015 Oct 15;571(1):151]
REFERENCE   6  (bases 1 to 893)
  AUTHORS   Lee,K.F., Simon,H., Chen,H., Bates,B., Hung,M.C. and Hauser,C.
  TITLE     Requirement for neuregulin receptor erbB2 in neural and cardiac
            development
  JOURNAL   Nature 378 (6555), 394-398 (1995)
   PUBMED   7477377
REFERENCE   7  (bases 1 to 893)
  AUTHORS   Lyons,I., Parsons,L.M., Hartley,L., Li,R., Andrews,J.E., Robb,L.
            and Harvey,R.P.
  TITLE     Myogenic and morphogenetic defects in the heart tubes of murine
            embryos lacking the homeo box gene Nkx2-5
  JOURNAL   Genes Dev 9 (13), 1654-1666 (1995)
   PUBMED   7628699
REFERENCE   8  (bases 1 to 893)
  AUTHORS   Lee,K.J., Ross,R.S., Rockman,H.A., Harris,A.N., O'Brien,T.X., van
            Bilsen,M., Shubeita,H.E., Kandolf,R., Brem,G., Price,J. et al.
  TITLE     Myosin light chain-2 luciferase transgenic mice reveal distinct
            regulatory programs for cardiac and skeletal muscle-specific
            expression of a single contractile protein gene
  JOURNAL   J Biol Chem 267 (22), 15875-15885 (1992)
   PUBMED   1379240
REFERENCE   9  (bases 1 to 893)
  AUTHORS   Hastings,K.E. and Emerson,C.P. Jr.
  TITLE     cDNA clone analysis of six co-regulated mRNAs encoding skeletal
            muscle contractile proteins
  JOURNAL   Proc Natl Acad Sci U S A 79 (5), 1553-1557 (1982)
   PUBMED   6951196
REFERENCE   10 (bases 1 to 893)
  AUTHORS   Czosnek,H., Nudel,U., Shani,M., Barker,P.E., Pravtcheva,D.D.,
            Ruddle,F.H. and Yaffe,D.
  TITLE     The genes coding for the muscle contractile proteins, myosin heavy
            chain, myosin light chain 2, and skeletal muscle actin are located
            on three different mouse chromosomes
  JOURNAL   EMBO J 1 (11), 1299-1305 (1982)
   PUBMED   6897916
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC140353.2.
            
            On Jun 16, 2020 this sequence version replaced XM_006530186.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BB661951.1, SRR5189679.191820.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849383, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AC140353.2         120298-120347
            51-140              AC140353.2         121067-121156
            141-216             AC140353.2         122014-122089
            217-321             AC140353.2         123149-123253
            322-400             AC140353.2         124145-124223
            401-449             AC140353.2         124306-124354
            450-568             AC140353.2         125989-126107
            569-893             AC140353.2         132467-132791
FEATURES             Location/Qualifiers
     source          1..893
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5"
     gene            1..893
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="myosin, light polypeptide 2, regulatory, cardiac,
                     slow"
                     /db_xref="GeneID:17906"
                     /db_xref="MGI:MGI:97272"
     exon            1..50
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     CDS             48..548
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="isoform 1 is encoded by transcript variant 2;
                     myosin light chain 2v; myosin light chain,
                     phosphorylatable, cardiac ventricles"
                     /codon_start=1
                     /product="myosin regulatory light chain 2,
                     ventricular/cardiac muscle isoform isoform 1"
                     /protein_id="NP_001371255.1"
                     /db_xref="GeneID:17906"
                     /db_xref="MGI:MGI:97272"
                     /translation="MAPKKAKKRIEGGSSNVFSMFEQTQIQEFKEAFTIMDQNRDGFI
                     DKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNA
                     FKVFDPEGKGSLKADYVREMLTTQAERFSKEEIDQMFAAFPPDVTGNLDYKNLVHIIT
                     HGEEKD"
     exon            51..140
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            141..216
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            217..321
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            322..400
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            401..449
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            450..568
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     exon            569..893
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /inference="alignment:Splign:2.1.0"
     regulatory      867..872
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="hexamer: CATAAA"
     polyA_site      893
                     /gene="Myl2"
                     /gene_synonym="Gm32672; MLC-2; MLC-2s/v; MLC-2v; Mlc2v;
                     Mylpc"
                     /note="major polyA site"
ORIGIN      
        1 agcaggctcc tcgaactctc cagaggtggc aactggcctc agacaccatg gcaccaaaga
       61 aagccaagaa gcggatagaa ggcgggagct ccaacgtgtt ctccatgttt gagcagaccc
      121 agatccagga gttcaaggaa gccttcacaa tcatggacca gaacagagac ggcttcatcg
      181 acaagaatga cctaagggac acatttgctg ccctaggacg agtgaacgtg aaaaatgaag
      241 agatcgatga aatgatcaaa gaggctccag gtccaattaa cttcaccgtg ttcctcacga
      301 tgtttgggga gaaacttaaa ggggctgatc ctgaagagac cattctcaac gcattcaagg
      361 tgtttgatcc cgagggcaaa gggtcactga aggctgacta tgtccgggag atgctgacca
      421 cacaagcaga gaggttctcc aaagaggaga tcgaccagat gttcgcagcc tttccccctg
      481 acgtcaccgg caatcttgat tataagaatt tggtccacat cattacccac ggagaagaga
      541 aggactgagc cctgaaccac agcctcagga tgctccgggt gccctcatgg gaggtctgct
      601 gctgtgaccg caagttgatg atgtcatcct gctcagacag acgagaacca gtaggagaag
      661 atgggagttg ccctcatcca ctgtatccaa ccctactcca tgtggggtaa cccatcaagg
      721 agtcgcccct gtccccacct tctcctcagg cattctattc ttcatcctct gagaatcatc
      781 tagaactatc tagtgcagta gctctcaacc ttcctaatgc ctcgaccctt taatatagtt
      841 ccttcatgtt gtggtgactc cccaaacata aaattatttc tgtcgctact tca
//
