LOCUS       NM_001303021             598 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus myosin, light chain 10, regulatory (Myl10), transcript
            variant 4, mRNA.
ACCESSION   NM_001303021
VERSION     NM_001303021.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 598)
  AUTHORS   Grossman,G.H., Ebke,L.A., Beight,C.D., Jang,G.F., Crabb,J.W. and
            Hagstrom,S.A.
  TITLE     Protein partners of dynamin-1 in the retina
  JOURNAL   Vis Neurosci 30 (4), 129-139 (2013)
   PUBMED   23746204
REFERENCE   3  (bases 1 to 598)
  AUTHORS   Oltz,E.M., Yancopoulos,G.D., Morrow,M.A., Rolink,A., Lee,G.,
            Wong,F., Kaplan,K., Gillis,S., Melchers,F. and Alt,F.W.
  TITLE     A novel regulatory myosin light chain gene distinguishes pre-B cell
            subsets and is IL-7 inducible
  JOURNAL   EMBO J 11 (7), 2759-2767 (1992)
   PUBMED   1628631
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ236701.1.
            
            Transcript Variant: This variant (4) uses an alternate 5' terminal
            exon resulting in a distinct 5' UTR and the use of an in-frame
            downstream start codon, compared to variant 1. This results in
            isoform 2 which has a shorter N-terminus compared to isoform 1.
            Variants 2 and 4 encode the same isoform (2).
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849383,
                              SAMN04095719 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-598               CJ236701.1         2-599
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 76.03 cM"
     gene            1..598
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /note="myosin, light chain 10, regulatory"
                     /db_xref="GeneID:59310"
                     /db_xref="MGI:MGI:1891705"
     exon            1..49
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /inference="alignment:Splign:2.1.0"
     exon            50..125
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /inference="alignment:Splign:2.1.0"
     CDS             62..457
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /note="isoform 2 is encoded by transcript variant 4;
                     myosin regulatory light chain 10; precursor
                     lymphocyte-specific regulatory light chain; myosin light
                     chain 2, precursor lymphocyte-specific"
                     /codon_start=1
                     /product="myosin regulatory light chain 10 isoform 2"
                     /protein_id="NP_001289950.1"
                     /db_xref="CCDS:CCDS80434.1"
                     /db_xref="GeneID:59310"
                     /db_xref="MGI:MGI:1891705"
                     /translation="MDQNRDGFIDKEDLRDTFAALGRINVKNEELEAMVKEAPGPINF
                     TVFLTMFGEKLKGTDPEETILHAFKVFDTEGKGFVKADFIKEKLMTQADRFSEEEVKQ
                     MFAAFPPDVCGNLDYRNLCYVITHGEEKD"
     exon            126..230
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /inference="alignment:Splign:2.1.0"
     exon            231..309
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /inference="alignment:Splign:2.1.0"
     exon            310..358
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /inference="alignment:Splign:2.1.0"
     exon            359..598
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      578..583
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /note="hexamer: AATAAA"
     polyA_site      598
                     /gene="Myl10"
                     /gene_synonym="1700027I08Rik; Mylc2pl; PLRLC"
                     /note="major polyA site"
ORIGIN      
        1 tcattcagtc aaaaacccag cccgtgggat agtgccgccc tcacttaagg ccttcactat
       61 catggaccag aaccgcgatg gcttcattga caaggaggac ctgagggaca cctttgctgc
      121 actgggtcgc ataaatgtca agaacgagga attggaagcc atggtgaagg aggctccggg
      181 gcccatcaac ttcacagtct tcctgaccat gtttggggag aaactcaagg gcacggaccc
      241 agaagagact atactgcacg ccttcaaagt gtttgacact gaaggaaaag gctttgtcaa
      301 ggctgacttc attaaggaaa agcttatgac gcaggctgac cggttcagtg aggaggaggt
      361 caagcagatg tttgcagctt tcccgccaga tgtctgtggc aacttagact acaggaatct
      421 gtgctacgtc atcacacacg gcgaggagaa ggactagatg tgtctcctcc atacctggag
      481 tcagccaaga tggggttggg tgggcagggg gcagtacagg gctatcatag gtagggaaac
      541 agtctccatc acccccggag tccggagctg aagtgggaat aaataacaca gagatgtg
//
