LOCUS       NM_010855               6037 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus myosin, heavy polypeptide 4, skeletal muscle (Myh4),
            mRNA.
ACCESSION   NM_010855 XM_126119
VERSION     NM_010855.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Kolonay,D.W., Sattler,K.M., Strawser,C., Rafael-Fortney,J.,
            Mihaylova,M.M., Miller,K.E., Lepper,C. and Baskin,K.K.
  TITLE     Temporal regulation of the Mediator complex during muscle
            proliferation, differentiation, regeneration, aging, and disease
  JOURNAL   Front Cell Dev Biol 12, 1331563 (2024)
   PUBMED   38690566
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  AUTHORS   Eguchi,T., Tezuka,T., Watanabe,Y., Inoue-Yamauchi,A., Sagara,H.,
            Ozawa,M. and Yamanashi,Y.
  TITLE     Calcium-binding protein 7 expressed in muscle negatively regulates
            age-related degeneration of neuromuscular junctions in mice
  JOURNAL   iScience 27 (2), 108997 (2024)
   PUBMED   38327785
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6037)
  AUTHORS   Jacobson,K.R., Saleh,A.M., Lipp,S.N., Tian,C., Watson,A.R.,
            Luetkemeyer,C.M., Ocken,A.R., Spencer,S.L., Kinzer-Ursem,T.L. and
            Calve,S.
  TITLE     Extracellular matrix protein composition dynamically changes during
            murine forelimb development
  JOURNAL   iScience 27 (2), 108838 (2024)
   PUBMED   38303699
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6037)
  AUTHORS   Dos Santos,M., Shah,A.M., Zhang,Y., Bezprozvannaya,S., Chen,K.,
            Xu,L., Lin,W., McAnally,J.R., Bassel-Duby,R., Liu,N. and Olson,E.N.
  TITLE     Opposing gene regulatory programs governing myofiber development
            and maturation revealed at single nucleus resolution
  JOURNAL   Nat Commun 14 (1), 4333 (2023)
   PUBMED   37468485
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6037)
  AUTHORS   So,H.K., Kim,H., Lee,J., You,C.L., Yun,C.E., Jeong,H.J., Jin,E.J.,
            Jo,Y., Ryu,D., Bae,G.U. and Kang,J.S.
  TITLE     Protein Arginine Methyltransferase 1 Ablation in Motor Neurons
            Causes Mitochondrial Dysfunction Leading to Age-related Motor
            Neuron Degeneration with Muscle Loss
  JOURNAL   Research (Wash D C) 6, 0158 (2023)
   PUBMED   37342629
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6037)
  AUTHORS   Dennehey,B.K., Leinwand,L.A. and Krauter,K.S.
  TITLE     Diversity in transcriptional start site selection and alternative
            splicing affects the 5'-UTR of mouse striated muscle myosin
            transcripts
  JOURNAL   J Muscle Res Cell Motil 27 (8), 559-575 (2006)
   PUBMED   16819597
REFERENCE   7  (bases 1 to 6037)
  AUTHORS   Lucas,M., Goblet,C., Keller,A., Lamande,N., Gros,F., Whalen,R.G.
            and Lazar,M.
  TITLE     Modulation of embryonic and muscle-specific enolase gene products
            in the developing mouse hindlimb
  JOURNAL   Differentiation 51 (1), 1-7 (1992)
   PUBMED   1280607
REFERENCE   8  (bases 1 to 6037)
  AUTHORS   Suter,U., Moskow,J.J., Welcher,A.A., Snipes,G.J., Kosaras,B.,
            Sidman,R.L., Buchberg,A.M. and Shooter,E.M.
  TITLE     A leucine-to-proline mutation in the putative first transmembrane
            domain of the 22-kDa peripheral myelin protein in the trembler-J
            mouse
  JOURNAL   Proc Natl Acad Sci U S A 89 (10), 4382-4386 (1992)
   PUBMED   1374899
REFERENCE   9  (bases 1 to 6037)
  AUTHORS   Smith,T.H. and Miller,J.B.
  TITLE     Distinct myogenic programs of embryonic and fetal mouse muscle
            cells: expression of the perinatal myosin heavy chain isoform in
            vitro
  JOURNAL   Dev Biol 149 (1), 16-26 (1992)
   PUBMED   1728586
REFERENCE   10 (bases 1 to 6037)
  AUTHORS   Cox,R.D., Weydert,A., Barlow,D. and Buckingham,M.E.
  TITLE     Three linked myosin heavy chain genes clustered within 370 kb of
            each other show independent transcriptional and
            post-transcriptional regulation during differentiation of a mouse
            muscle cell line
  JOURNAL   Dev Biol 143 (1), 36-43 (1991)
   PUBMED   1985022
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK138608.1 and AL596129.27.
            
            On Jan 30, 2013 this sequence version replaced NM_010855.2.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223969.10804.1,
                                           SRR10223970.13587.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2732              AK138608.1         2-2733
            2733-2801           AL596129.27        153465-153533
            2802-3044           AL596129.27        154232-154474
            3045-3221           AL596129.27        154708-154884
            3222-3367           AL596129.27        155081-155226
            3368-3458           AL596129.27        155499-155589
            3459-3848           AL596129.27        155680-156069
            3849-3975           AL596129.27        156526-156652
            3976-4094           AL596129.27        156984-157102
            4095-4291           AL596129.27        157203-157399
            4292-4475           AL596129.27        157825-158008
            4476-4641           AL596129.27        158094-158259
            4642-4766           AL596129.27        158358-158482
            4767-5075           AL596129.27        158713-159021
            5076-5279           AL596129.27        159618-159821
            5280-5405           AL596129.27        161139-161264
            5406-5576           AL596129.27        161352-161522
            5577-5681           AL596129.27        161609-161713
            5682-5777           AL596129.27        161816-161911
            5778-6037           AL596129.27        162714-162973
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 40.59 cM"
     gene            1..6037
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /note="myosin, heavy polypeptide 4, skeletal muscle"
                     /db_xref="GeneID:17884"
                     /db_xref="MGI:MGI:1339713"
     exon            1..22
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            23..71
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            72..314
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     CDS             111..5930
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /note="myHC-2b; myosin heavy chain 4; myosin heavy chain
                     2b"
                     /codon_start=1
                     /product="myosin-4"
                     /protein_id="NP_034985.2"
                     /db_xref="CCDS:CCDS24856.1"
                     /db_xref="GeneID:17884"
                     /db_xref="MGI:MGI:1339713"
                     /translation="MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAK
                     ESYVKATVQSREGGKVTAKTEGGATVTVKDDQVFSMNPPKYDKIEDMAMMTHLHEPAV
                     LYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVAAYRGKKRQEAPPHIFSISD
                     NAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKEEATSGKMQGT
                     LEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRV
                     TFQLKAERSYHIFYQIMSNKKPELIEMLLITTNPYDFAYVSQGEITVPSIDDQEELMA
                     TDTAVDILGFSADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTS
                     LNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALAKSMYEKMFLWMVTRINQQL
                     DTKQPRQYFIGVLDIAGFEIFDFNTLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEG
                     IDWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNF
                     QKPKPAKGKAEAHFSLVHYAGTVDYNIIGWLDKNKDPLNETVVGLYQKSGLKTLAFLF
                     SGGQAAEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLKSTHPHFVRCLIPNET
                     KTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFI
                     DSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAVCRG
                     YLMRVEFKKMMERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMANM
                     KEDFEKAKEDLAKSEAKRKELEEKMVALMQEKNDLQLQVQAEADGLADAEERCDQLIK
                     TKIQLEAKIKELTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEK
                     HATENKVKNLTEEMAGLDENIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTK
                     LEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKE
                     FEMSNLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRE
                     LEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSV
                     AELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMETVSKAKGNLEKMCRTLEDQLSE
                     VKTKEEEQQRLINELSTQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELK
                     RQLEEESKAKNALAHALQSARHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTK
                     YETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDV
                     ERSNAACAALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEE
                     SLDQLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKIKKQIDQEKSELQASLEEAE
                     ASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRVVESMQSTLDAEIR
                     SRNDALRIKKKMEGDLNEMEIQLNHANRQAAEAIRNLRNTQGMLKDTQLHLDDALRGQ
                     DDLKEQLAMVERRANLMQAEIEELRASLEQTERSRRVAEQELLDASERVQLLHTQNTS
                     LINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLE
                     RMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELENEVENEQKRNIEAVK
                     GLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKF
                     RKIQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE"
     exon            315..458
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            459..615
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            616..643
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            644..758
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            759..851
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            852..915
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1014
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1015..1118
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1257
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1376
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1377..1526
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1697
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..2007
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2008..2078
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2079..2166
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2167..2284
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2285..2408
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2545
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2546..2801
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            2802..3044
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            3045..3221
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            3222..3367
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            3368..3458
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            3459..3848
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            3849..3975
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            3976..4094
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            4095..4291
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            4292..4475
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            4476..4641
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            4642..4766
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            4767..5075
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            5076..5279
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            5280..5405
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            5406..5576
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            5577..5681
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            5682..5777
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     exon            5778..6037
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6007..6012
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /note="hexamer: AATAAA"
     polyA_site      6036
                     /gene="Myh4"
                     /gene_synonym="MHC2B; Minimsc; Minmus; MM; MYH-2B;
                     MyHC-IIb; Myhsf"
                     /note="major polyA site"
ORIGIN      
        1 gtccttcctc aaacccttaa agtacttgtc tgactcaagc ctgcctcctt cttcatctgg
       61 taacacaaga ggtgcacctg gccctgagct gccaccaata gcccgcagcc atgagttccg
      121 acgctgagat ggccgttttc ggggaggctg ctccttacct ccggaagtct gaaaaggagc
      181 gaatcgaggc ccagaacaag ccttttgatg ccaagtcatc ggtgtttgtg gtggatgcta
      241 aggagtccta tgtgaaagca acagtgcaga gcagggaagg ggggaaggtg acagccaaga
      301 ccgaaggcgg agctacggtc accgtcaagg atgatcaagt cttctccatg aaccctccca
      361 agtacgacaa gattgaggac atggccatga tgacccacct gcacgagccc gctgtgctgt
      421 acaacctcaa agagcgttat gcagcctgga tgatctacac ttactcaggc ctcttttgtg
      481 tcaccgtcaa cccctacaag tggctgccgg tgtacaaccc tgaggtggtg gcagcctacc
      541 gaggcaaaaa gcgccaggag gccccacccc acatcttctc catctctgat aacgcctacc
      601 agttcatgct gacggatcgt gagaatcagt ccatcctgat tactggagaa tccggggccg
      661 ggaagactgt gaacacgaag cgtgtcatcc agtactttgc aacaattgca gtcactgggg
      721 acaagaagaa ggaggaggcg acttctggca aaatgcaggg gacccttgaa gatcaaatta
      781 tcagtgccaa ccccctgctg gaggccttcg gcaacgccaa gactgtgagg aacgacaact
      841 cttctcgatt tggtaaattc atcagaatcc atttcggtgc cacaggcaaa ctggcctctg
      901 cagatattga aacctatctg ctagagaagt ccagagtcac cttccagctc aaggctgaaa
      961 ggagctacca catcttctac caaatcatgt ccaataagaa accagagctt attgagatgc
     1021 ttctgatcac caccaaccca tatgactttg cttacgtcag tcaaggtgaa atcacagtgc
     1081 ccagcattga tgaccaggaa gagctgatgg ccacagacac tgctgttgat atcctgggat
     1141 tcagtgcaga tgaaaaggtg gccatttaca agctcacagg cgctgtgatg cattatggga
     1201 acatgaaatt caagcaaaag caaagggaag agcaggctga gccagacggc accgaagttg
     1261 ctgacaaggc tgcctatctg acaagtctga actctgctga cctgctcaaa gccctctgct
     1321 accccagggt caaggtcggc aatgagtatg tcaccaaagg ccagacggtg cagcaggtgt
     1381 acaactccgt gggtgccctg gccaagtcca tgtatgagaa gatgttccta tggatggtca
     1441 cccgcatcaa ccagcagctg gacaccaagc agccccggca gtacttcatc ggggtcttgg
     1501 acattgctgg ctttgagatc tttgacttca acaccttgga gcagctgtgc atcaacttca
     1561 ccaatgagaa actgcaacag tttttcaacc accacatgtt cgtgctggag caggaggagt
     1621 acaagaagga gggcattgat tgggagttca ttgacttcgg gatggacctg gcggcctgca
     1681 tcgagctcat cgagaagccg atgggcatct tctccatcct ggaagaggag tgcatgttcc
     1741 ctaaggcgac agacacctcc ttcaagaaca agctgtatga gcagcatctt ggaaagtcca
     1801 acaacttcca gaagcccaaa cccgccaaag gcaaggctga ggctcacttc tccctggtgc
     1861 actatgcggg caccgtggac tacaacatta ttggctggct ggacaagaac aaggaccccc
     1921 tgaatgagac cgtggtgggg ctgtaccaga aatccgggtt gaagactctg gctttcctat
     1981 tttctggggg acaagctgcg gaagcagagg gcggcggtgg aaagaaaggt ggcaagaaga
     2041 agggttcttc tttccagacc gtgtcagctc tcttcaggga gaatttaaat aagctgatga
     2101 ccaacttgaa gagcacccac ccccactttg tcagatgcct cattcccaat gaaactaaga
     2161 ctcctggtgc catggagcat gaactggtct tgcaccagct caggtgtaac ggggtgctgg
     2221 aaggcatccg catctgtagg aaggggttcc ccagcaggat cctctatgca gacttcaagc
     2281 agagatacaa agtcctgaat gcaagtgcca tccctgaggg tcagttcatt gatagcaaga
     2341 aggcctctga gaaacttctg ggttccattg acattgacca cactcagtac aaattcggtc
     2401 acactaaggt tttctttaaa gccggcctgt tgggaactct agaggagatg cgagatgaaa
     2461 agctggctca gctcatcacc cgcactcaag ccgtgtgcag agggtacctg atgagggtgg
     2521 agttcaaaaa gatgatggag aggagagagt ccatcttctg cattcagtac aacgtccgcg
     2581 ccttcatgaa tgtgaaacac tggccctgga tgaagctgta tttcaagatc aaacctctgc
     2641 tgaagagcgc ggagaccgag aaggagatgg ccaacatgaa ggaagatttt gagaaggcca
     2701 aggaggatct ggctaagtca gaggcaaaaa ggaaggaact agaagaaaag atggtagctt
     2761 tgatgcagga gaaaaatgac ctgcagctcc aggttcaagc tgaagcagat ggcttggctg
     2821 atgctgagga aaggtgcgac cagctgatca aaaccaaaat ccagctggag gccaaaatca
     2881 aagagctgac cgagagggcc gaggacgagg aggagatcaa cgcggagctc acggccaaga
     2941 agaggaagct ggaggacgag tgctcagagc tgaagaagga cattgacgac cttgagctga
     3001 cactggccaa ggttgagaag gagaagcacg ccacggagaa caaggtgaaa aacctgacag
     3061 aagagatggc aggcctggat gaaaatatcg ccaagctgac caaggagaag aaggccctcc
     3121 aggaggccca ccagcagacc ctggatgacc tgcaggcaga ggaggacaaa gtcaacaccc
     3181 tgaccaaagc caaaaccaag ctggaacagc aagtggatga ccttgaagga tcactggaac
     3241 aggaaaagaa gctgaggatg gacttagaaa gagccaagag gaaactggag ggggacctga
     3301 aactggccca agagtccaca atggacatag aaaacgataa acagcagctc gatgagaaac
     3361 tcaaaaagaa agagtttgaa atgagcaatc tgcagagcaa gattgaggac gagcaggccc
     3421 tcggcatgca gctgcagaag aagatcaagg agttgcaggc ccgcatcgag gagctggagg
     3481 aggaaatcga ggcagagcgg gcctccaggg ctaaagcaga gaagcagcgc tctgacctct
     3541 cccgggaact ggaggagatc agcgagaggc tggaagaagc tggcggagcc acttcagccc
     3601 agatcgagat gaacaagaag agagaggctg agttccagaa aatgcgcagg gacctggagg
     3661 aggccacgct gcagcatgaa gccacagcag ccgctcttcg gaagaagcac gcggacagcg
     3721 tggctgagct cggggagcag atcgacaacc tgcagcgggt gaagcagaag ctggagaagg
     3781 agaagagcga gctgaagatg gagatcgatg atctcgctag taacatggag acagtctcca
     3841 aagccaaggg gaaccttgag aagatgtgcc gcaccctgga ggaccaactg agtgaagtga
     3901 agaccaagga ggaggagcag cagcgcctga tcaatgaact gtcaacccag aaggcacgct
     3961 tgcacacaga gtcaggcgag ttttcccgac agctggatga aaaagacgct atggtttctc
     4021 agctttcccg aggcaaacaa gcatttacac aacagattga ggaactaaag aggcagctag
     4081 aggaagagtc caaggccaag aacgcactgg cccacgccct gcagtcagct cgccatgact
     4141 gtgacctgct gcgggaacag tatgaagagg agcaggaagc caaggctgag ctgcagaggg
     4201 ccatgtccaa ggccaacagc gaggtagccc agtggaggac gaaatatgag acggatgcca
     4261 tccagcgcac agaggagctg gaagaggcca agaagaagct ggctcagcgt ctgcaggatg
     4321 ctgaggaaca tgtagaagct gtgaattcca aatgcgcttc tcttgaaaag acgaagcagc
     4381 ggcttcagaa tgaagtggag gacctcatga ttgacgtgga gaggtctaac gctgcctgcg
     4441 ctgcgcttga taagaagcag aggaactttg acaaggtcct ggcagaatgg aaacagaagt
     4501 acgaggaaac tcaggctgag cttgaagcct cccagaagga gtcccgctct ctgagcactg
     4561 agctgttcaa agtgaagaat gcctatgagg agtctctgga tcaacttgag acgttaaaga
     4621 gagagaacaa gaacctgcag caggagatct ctgacctgac cgagcagatt gctgagggag
     4681 ggaagcacat ccatgagctg gagaaaatca agaagcaaat tgatcaggaa aagagtgaat
     4741 tacaggcttc tctggaggaa gcggaggcat cccttgaaca tgaagaaggt aaaatcctac
     4801 gcatccagct ggagttgaac caggtgaaat ctgagattga ccgcaaaatt gctgaaaaag
     4861 atgaagagat agatcagctg aagagaaacc acctcagagt tgtggagtcc atgcagagca
     4921 cgctggacgc cgagatccgc agcaggaatg atgctctgag gattaagaag aagatggagg
     4981 gagacctcaa tgagatggag atccagctga accatgccaa ccgccaggct gcggaggcaa
     5041 tcaggaacct tcggaacacc cagggaatgc tgaaggacac acagctgcac ctggacgatg
     5101 ctctcagagg ccaggacgac ctgaaagagc agctggccat ggttgagcgc agagccaacc
     5161 tgatgcaggc tgagatcgag gagctcaggg catccctgga acagacagag aggagcagga
     5221 gagtggccga gcaagagcta ctggatgcca gtgagcgcgt gcagctcctc cacacccaga
     5281 acaccagcct catcaacacc aagaagaagc tggagacaga catttcccaa atccagggag
     5341 agatggaaga catcgtccag gaagcccgca acgcagaaga gaaggccaag aaagccatca
     5401 ctgatgccgc catgatggcg gaggagctga agaaggagca ggacaccagc gcccacctgg
     5461 agcggatgaa gaagaacatg gagcagaccg tgaaggacct gcagcaccgt ctggacgagg
     5521 ctgagcagct ggcgctgaag ggcggcaaga agcagatcca gaaactggag gctagggtga
     5581 gggagcttga aaacgaggtg gaaaatgaac agaagcgcaa catcgaagcc gtcaagggtc
     5641 ttcgtaagca cgagcgcaga gtgaaggaac tcacctacca gaccgaggag gaccgcaaga
     5701 acgtgctgag gctgcaggac ttggtggaca aactacagac taaagtgaaa gcctacaaga
     5761 gacaggctga ggaggctgag gaacaatcca atgtcaacct ggccaagttc cgtaagatcc
     5821 agcacgagct ggaggaagcc gaggagcggg ctgacatcgc ggagtcccag gtcaacaagc
     5881 tgcgggtgaa gagccgagag gttcacacta aagtcataag cgaagaataa tccatctttc
     5941 tgttgagagg tgacaggaga aatcacaaaa tgtgacgttc tttgtcactg tcctgtatat
     6001 cacggaaata aattctgcag ataattttgc aatctag
//
