LOCUS       NM_001012323             902 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus major urinary protein 20 (Mup20), mRNA.
ACCESSION   NM_001012323 XM_355497
VERSION     NM_001012323.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Demir,E., Li,K., Bobrowski-Khoury,N., Sanders,J.I., Beynon,R.J.,
            Hurst,J.L., Kepecs,A. and Axel,R.
  TITLE     The pheromone darcin drives a circuit for innate and reinforced
            behaviours
  JOURNAL   Nature 578 (7793), 137-141 (2020)
   PUBMED   31996852
REFERENCE   2  (bases 1 to 902)
  AUTHORS   Charkoftaki,G., Wang,Y., McAndrews,M., Bruford,E.A., Thompson,D.C.,
            Vasiliou,V. and Nebert,D.W.
  TITLE     Update on the human and mouse lipocalin (LCN) gene family,
            including evidence the mouse Mup cluster is result of an
            'evolutionary bloom'
  JOURNAL   Hum Genomics 13 (1), 11 (2019)
   PUBMED   30782214
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 902)
  AUTHORS   Ricatti,J., Acquasaliente,L., Ribaudo,G., De Filippis,V.,
            Bellini,M., Llovera,R.E., Barollo,S., Pezzani,R., Zagotto,G.,
            Persaud,K.C. and Mucignat-Caretta,C.
  TITLE     Effects of point mutations in the binding pocket of the mouse major
            urinary protein MUP20 on ligand affinity and specificity
  JOURNAL   Sci Rep 9 (1), 300 (2019)
   PUBMED   30670733
  REMARK    GeneRIF: 14 critical amino acids in the binding pocket involved in
            ligand interactions were identified in MUP20 using in silico
            techniques and 7 MUP20 mutants were synthesized and characterized
            to produce a set of proteins with diverse ligand binding profiles
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 902)
  AUTHORS   Liu,Y.J., Guo,H.F., Zhang,J.X. and Zhang,Y.H.
  TITLE     Quantitative inheritance of volatile pheromones and darcin and
            their interaction in olfactory preferences of female mice
  JOURNAL   Sci Rep 7 (1), 2094 (2017)
   PUBMED   28522864
  REMARK    Erratum:[Sci Rep. 2018 Mar 6;8(1):4300. doi:
            10.1038/s41598-018-22515-2.. PMID: 29511252]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 902)
  AUTHORS   Jiang,J., Creasy,K.T., Purnell,J., Peterson,M.L. and Spear,B.T.
  TITLE     Zhx2 (zinc fingers and homeoboxes 2) regulates major urinary
            protein gene expression in the mouse liver
  JOURNAL   J Biol Chem 292 (16), 6765-6774 (2017)
   PUBMED   28258223
REFERENCE   6  (bases 1 to 902)
  AUTHORS   Roberts,S.A., Davidson,A.J., McLean,L., Beynon,R.J. and Hurst,J.L.
  TITLE     Pheromonal induction of spatial learning in mice
  JOURNAL   Science 338 (6113), 1462-1465 (2012)
   PUBMED   23239735
REFERENCE   7  (bases 1 to 902)
  AUTHORS   Roberts,S.A., Simpson,D.M., Armstrong,S.D., Davidson,A.J.,
            Robertson,D.H., McLean,L., Beynon,R.J. and Hurst,J.L.
  TITLE     Darcin: a male pheromone that stimulates female memory and sexual
            attraction to an individual male's odour
  JOURNAL   BMC Biol 8, 75 (2010)
   PUBMED   20525243
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 902)
  AUTHORS   Logan,D.W., Marton,T.F. and Stowers,L.
  TITLE     Species specificity in major urinary proteins by parallel evolution
  JOURNAL   PLoS One 3 (9), e3280 (2008)
   PUBMED   18815613
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 902)
  AUTHORS   Chamero,P., Marton,T.F., Logan,D.W., Flanagan,K., Cruz,J.R.,
            Saghatelian,A., Cravatt,B.F. and Stowers,L.
  TITLE     Identification of protein pheromones that promote aggressive
            behaviour
  JOURNAL   Nature 450 (7171), 899-902 (2007)
   PUBMED   18064011
REFERENCE   10 (bases 1 to 902)
  AUTHORS   Armstrong,S.D., Robertson,D.H., Cheetham,S.A., Hurst,J.L. and
            Beynon,R.J.
  TITLE     Structural and functional differences in isoforms of mouse major
            urinary proteins: a male-specific protein that preferentially binds
            a male pheromone
  JOURNAL   Biochem J 391 (Pt 2), 343-350 (2005)
   PUBMED   15934926
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC089613.1.
            
            On Feb 17, 2005 this sequence version replaced XM_355497.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC089613.1, CA479582.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849375,
                                           SAMN00849386 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 32.98 cM"
     gene            1..902
                     /gene="Mup20"
                     /gene_synonym="darcin; Gm12560; Mup24"
                     /note="major urinary protein 20"
                     /db_xref="GeneID:381530"
                     /db_xref="MGI:MGI:3651981"
     exon            1..167
                     /gene="Mup20"
                     /gene_synonym="darcin; Gm12560; Mup24"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..614
                     /gene="Mup20"
                     /gene_synonym="darcin; Gm12560; Mup24"
                     /note="major urinary protein 24"
                     /codon_start=1
                     /product="major urinary protein 20 precursor"
                     /protein_id="NP_001012323.1"
                     /db_xref="CCDS:CCDS18233.1"
                     /db_xref="GeneID:381530"
                     /db_xref="MGI:MGI:3651981"
                     /translation="MKLLVLLLCLGLTLVCVHAEEASSMERNFNVEKINGEWYTIMLA
                     TDKREKIEEHGSMRVFVEYIHVLENSLALKFHIIINEECSEIFLVADKTEKAGEYSVT
                     YDGSNTFTILKTDYDNYIMIHLINKKDGETFQLMELYGREPDLSSDIKEKFAQLSEEH
                     GIVRENIIDLTNANRCLEARE"
     exon            168..301
                     /gene="Mup20"
                     /gene_synonym="darcin; Gm12560; Mup24"
                     /inference="alignment:Splign:2.1.0"
     exon            302..375
                     /gene="Mup20"
                     /gene_synonym="darcin; Gm12560; Mup24"
                     /inference="alignment:Splign:2.1.0"
     exon            376..486
                     /gene="Mup20"
                     /gene_synonym="darcin; Gm12560; Mup24"
                     /inference="alignment:Splign:2.1.0"
     exon            487..588
                     /gene="Mup20"
                     /gene_synonym="darcin; Gm12560; Mup24"
                     /inference="alignment:Splign:2.1.0"
     exon            589..634
                     /gene="Mup20"
                     /gene_synonym="darcin; Gm12560; Mup24"
                     /inference="alignment:Splign:2.1.0"
     exon            635..887
                     /gene="Mup20"
                     /gene_synonym="darcin; Gm12560; Mup24"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gtggagtgta ggcaccatca ccagaaagac gtggttctga caggcagaaa attttactat
       61 ctaccaaaat gaagctgctg gtgctgctgc tgtgtttggg actgacccta gtctgtgtcc
      121 atgcagaaga agctagttct atggaaagga actttaatgt agaaaagatt aatggggaat
      181 ggtatactat tatgctggcc actgacaaaa gagaaaagat agaagaacat ggcagcatga
      241 gagtttttgt cgagtacatc catgtcttgg agaattcctt agctcttaaa ttccatatta
      301 ttataaatga ggagtgctct gaaatatttt tggttgctga caaaacagaa aaggctggtg
      361 aatattctgt aacgtatgat ggatcgaata catttactat acttaagaca gactatgata
      421 attatattat gattcatctc attaacaaaa aggatggaga aaccttccag ctgatggagc
      481 tctatggccg agaaccagat ttgagttcag acatcaagga aaagtttgca caactaagcg
      541 aggagcatgg aatcgttaga gaaaatatca ttgacctaac caatgccaat cgctgccttg
      601 aggcccgaga atgaagaatg gcctgagcct ccagtgttga gtggagactt ctcaccagga
      661 ctccaggatc agtccttcct atccatacag catccagtaa gaagttctgt gatctgcttt
      721 ccatcgtgtc ttacagagaa gtgcaatccc tgtctctcca gcatgttact aggataactc
      781 atcaagaatc aaagtctttc tttaaatttc tctttgacat acccatgaca attctccatg
      841 aatttcttcc tcttcctgtt caataaatga ttacacttgc acttaaaaaa aaaaaaaaaa
      901 aa
//
