LOCUS       NM_080457              12452 bp    mRNA    linear   ROD 30-DEC-2022
DEFINITION  Mus musculus mucin 4 (Muc4), mRNA.
ACCESSION   NM_080457
VERSION     NM_080457.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Biering SB, Sarnik SA, Wang E, Zengel JR, Leist SR, Schafer A,
            Sathyan V, Hawkins P, Okuda K, Tau C, Jangid AR, Duffy CV, Wei J,
            Gilmore RC, Alfajaro MM, Strine MS, Nguyenla X, Van Dis E, Catamura
            C, Yamashiro LH, Belk JA, Begeman A, Stark JC, Shon DJ, Fox DM,
            Ezzatpour S, Huang E, Olegario N, Rustagi A, Volmer AS,
            Livraghi-Butrico A, Wehri E, Behringer RR, Cheon DJ, Schaletzky J,
            Aguilar HC, Puschnik AS, Button B, Pinsky BA, Blish CA, Baric RS,
            O'Neal WK, Bertozzi CR, Wilen CB, Boucher RC, Carette JE, Stanley
            SA, Harris E, Konermann S and Hsu PD.
  TITLE     Genome-wide bidirectional CRISPR screens identify mucins as host
            factors modulating SARS-CoV-2 infection
  JOURNAL   Nat Genet 54 (8), 1078-1089 (2022)
   PUBMED   35879412
REFERENCE   2  (bases 1 to 12452)
  AUTHORS   Pothuraju R, Pai P, Chaudhary S, Siddiqui JA, Cox JL, Kaur S,
            Rachagani S, Roy HK, Bouvet M and Batra SK.
  TITLE     Depletion of transmembrane mucin 4 (Muc4) alters intestinal
            homeostasis in a genetically engineered mouse model of colorectal
            cancer
  JOURNAL   Aging (Albany NY) 14 (5), 2025-2046 (2022)
   PUBMED   35255004
  REMARK    GeneRIF: Depletion of transmembrane mucin 4 (Muc4) alters
            intestinal homeostasis in a genetically engineered mouse model of
            colorectal cancer.
REFERENCE   3  (bases 1 to 12452)
  AUTHORS   Timmermans S and Libert C.
  TITLE     Overview of inactivating mutations in the protein-coding genome of
            the mouse reference strain C57BL/6J
  JOURNAL   JCI Insight 3 (13), 121758 (2018)
   PUBMED   29997285
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12452)
  AUTHORS   Metwalli KA, Do MA, Nguyen K, Mallick S, Kin K, Farokhnia N, Jun G
            and Fakhouri WD.
  TITLE     Interferon Regulatory Factor 6 Is Necessary for Salivary Glands and
            Pancreas Development
  JOURNAL   J Dent Res 97 (2), 226-236 (2018)
   PUBMED   28898113
REFERENCE   5  (bases 1 to 12452)
  AUTHORS   Rowson-Hodel AR, Wald JH, Hatakeyama J, O'Neal WK, Stonebraker JR,
            VanderVorst K, Saldana MJ, Borowsky AD, Sweeney C and Carraway KL
            3rd.
  TITLE     Membrane Mucin Muc4 promotes blood cell association with tumor
            cells and mediates efficient metastasis in a mouse model of breast
            cancer
  JOURNAL   Oncogene 37 (2), 197-207 (2018)
   PUBMED   28892049
  REMARK    GeneRIF: Observations suggest that Muc4 may facilitate metastasis
            by promoting the association of circulating tumor cells with blood
            cells to augment tumor cell survival in circulation.
REFERENCE   6  (bases 1 to 12452)
  AUTHORS   Jonckheere N, Vincent A, Perrais M, Ducourouble MP, Male AK, Aubert
            JP, Pigny P, Carraway KL, Freund JN, Renes IB and Van Seuningen I.
  TITLE     The human mucin MUC4 is transcriptionally regulated by
            caudal-related homeobox, hepatocyte nuclear factors, forkhead box
            A, and GATA endodermal transcription factors in epithelial cancer
            cells
  JOURNAL   J Biol Chem 282 (31), 22638-22650 (2007)
   PUBMED   17553805
  REMARK    GeneRIF: MUC4 is regulated at the transcriptional level by CDX-1
            and -2, HNF-1 alpha and -1 beta, FOXA1/A2, HNF-4 alpha and -4
            gamma, and GATA-4, -5, and -6 factors in a cell-specific manner
REFERENCE   7  (bases 1 to 12452)
  AUTHORS   Garrison WD, Battle MA, Yang C, Kaestner KH, Sladek FM and Duncan
            SA.
  TITLE     Hepatocyte nuclear factor 4alpha is essential for embryonic
            development of the mouse colon
  JOURNAL   Gastroenterology 130 (4), 1207-1220 (2006)
   PUBMED   16618389
REFERENCE   8  (bases 1 to 12452)
  AUTHORS   Zhang J, Perez A, Yasin M, Soto P, Rong M, Theodoropoulos G,
            Carothers Carraway CA and Carraway KL.
  TITLE     Presence of MUC4 in human milk and at the luminal surfaces of blood
            vessels
  JOURNAL   J Cell Physiol 204 (1), 166-177 (2005)
   PUBMED   15672420
  REMARK    GeneRIF: As a luminal surface component, the MUC4 is situated to
            contribute to the non-adhesive luminal surface and to act as an
            intrinsic protection and survival factor for blood vessels
REFERENCE   9  (bases 1 to 12452)
  AUTHORS   Lee CG, Homer RJ, Cohn L, Link H, Jung S, Craft JE, Graham BS,
            Johnson TR and Elias JA.
  TITLE     Transgenic overexpression of interleukin (IL)-10 in the lung causes
            mucus metaplasia, tissue inflammation, and airway remodeling via
            IL-13-dependent and -independent pathways
  JOURNAL   J Biol Chem 277 (38), 35466-35474 (2002)
   PUBMED   12107190
REFERENCE   10 (bases 1 to 12452)
  AUTHORS   Desseyn JL, Clavereau I and Laine A.
  TITLE     Cloning, chromosomal localization and characterization of the
            murine mucin gene orthologous to human MUC4
  JOURNAL   Eur J Biochem 269 (13), 3150-3159 (2002)
   PUBMED   12084055
  REMARK    GeneRIF: full coding sequence was cloned and mapped to chromosome
            16, it's highest levels of expression are in trachea and intestinal
            tract
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC161755.3 and MG954261.1.
            
            [WARNING] On Apr 13, 2023 this sequence was replaced by
            NM_080457.5.
            
            On Jul 30, 2020 this sequence version replaced NM_080457.3.
            
            Summary: The major constituents of mucus, the viscous secretion
            that covers epithelial surfaces such as those in the trachea,
            colon, and cervix, are highly glycosylated proteins called mucins.
            These glycoproteins play important roles in the protection of the
            epithelial cells and have been implicated in epithelial renewal and
            differentiation. This gene encodes an integral membrane
            glycoprotein found on the cell surface. A large 5' exon encodes at
            least 15 tandem repeats of 124-126 amino acids. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments. The RefSeq includes an unresolved
            tandem repeat and may not correctly represent the genomic sequence.
            Two deletions were introduced in the repeat region compared to the
            reference genome to maintain the reading frame compared to mouse
            and human protein data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849377,
                              SAMN00849378 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-182               AC161755.3         8214-8395           c
            183-8666            MG954261.1         15380-23863
            8667-8795           MG954261.1         24087-24215
            8796-8929           MG954261.1         25924-26057
            8930-9094           MG954261.1         26817-26981
            9095-9250           MG954261.1         27927-28082
            9251-9381           MG954261.1         29173-29303
            9382-9470           MG954261.1         30650-30738
            9471-9650           MG954261.1         32423-32602
            9651-9767           MG954261.1         32825-32941
            9768-9887           MG954261.1         33624-33743
            9888-10099          MG954261.1         34043-34254
            10100-10184         MG954261.1         35430-35514
            10185-10352         MG954261.1         35878-36045
            10353-10454         MG954261.1         36491-36592
            10455-10688         MG954261.1         36944-37177
            10689-10826         MG954261.1         38314-38451
            10827-11008         MG954261.1         41474-41655
            11009-11168         MG954261.1         42494-42653
            11169-11342         MG954261.1         43526-43699
            11343-11416         MG954261.1         44395-44468
            11417-11487         MG954261.1         45488-45558
            11488-11723         MG954261.1         45638-45873
            11724-11886         MG954261.1         47533-47695
            11887-12452         MG954261.1         48561-49126
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16"
     gene            1..12452
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /note="mucin 4"
                     /db_xref="GeneID:140474"
                     /db_xref="MGI:MGI:2153525"
     exon            1..182
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..12082
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /note="testis mucin; ascites sialoglycoprotein; pancreatic
                     adenocarcinoma mucin; mucin 4, ASGP"
                     /codon_start=1
                     /product="mucin-4 precursor"
                     /protein_id="NP_536705.4"
                     /db_xref="GeneID:140474"
                     /db_xref="MGI:MGI:2153525"
                     /translation="MRGPHWRVPWLCLSCLYSCLLLLPDALATTSTQTPMSLSSSTRT
                     SQMSSQASTSSTSSDRRTSKTEQTSTRDTPSSITTVSQSHHTTSMETSKPQTTTTTEV
                     TTSTPSASSRDQIQTETSSQRTISPDGTTTSHAPSISSSAPSTTHMLTTTSSTESTSV
                     DSGHTTAITTQGLTPATAQVSLTPSSQNMSTVSTPITSTLTQRQHTGSKQTSSKSQVN
                     IVTSTLSTSTSDSTPAQTMSQVTSSSDKRTKPSTSGVSSTSLTTTEVLTQTSSTDSAP
                     GNTTLRITQNSTTHTTKVSTTSTPQKLSPVSTLINSSQKMSTLPQNQHTESMDTSRQP
                     QTTTTIEVTTSTPSASSLHQIQTETNSPKTISPGETTTSHAPNMRSSPPKTSQILTTM
                     PSTKSTSVDTKQTKAITTKVSTPDTTQVSMTPSSQKLPTHSTSTQELTSSYSQHIQSK
                     GTSSKSQTTTNTKVNTSTPSASSRDKIQTETSSQRTNSPGEKRTSHAPSMSSSAPSTT
                     HMLSTTSSNQSTSVDTGQTTSVTAQGSTPAITQTSLTPSSQNTSTVSTPITSTHKLST
                     LSQSQHTGSKGTSSNPQTTTTTEVTTSTPSATTHDQIQTETSSQNTISPGETTTSYAP
                     IMSSSAPSTTHMLSTTSSTQSTSVDTRHTTTLTNQGSTPATTQVSPSSQNMSTVSAPI
                     TSTQILSTFPQSQHTGSKGTSSNPQTTTTPVVTTSNPSATSRDQIQTETSSQRTISPG
                     ETTTSYASIMSSSAPSTTHMLTTTSSTQSTSVDTRHTIAVRTQGSTPATTQVSPSSQN
                     MSTVSTPITTTHKLSTLSQSQHTGSKGTSSNPQTTTTPVMTTSTPSATTHDQIQTETS
                     SQRTISHGETTTSYAPIMSSSAPSTTHMLSTASSTQITSVDTRHTTAITTQGSTPATT
                     QVSPSSQNMSTVSAPITSIQILSTLLQSQHTGSKGTSTKPQTTTTPVVTTSNPSATFP
                     DQFQTETSSQRKISPGETTTSHAPIMSSSAPSTTHMLSTTSSTQSTSVDTRHTTTVTT
                     QGSTPATTQVLPSSQNMSTVSAPITSTQILSTLPQSQHTGSKGTSTNPQTTTNAEVTT
                     STPSATSHDQIETETSSQRTISPGETTTSNASNLSSSAPSTTHMLTTTSSTQSTSVDT
                     GYTTAVRTQGSTPATTQVSPSSQNMSTVSAPITSTQILSTLPQSQHTGSKGTSSNPQT
                     TTSPVVTTSTPSGTSGDQIQTETSSQRTISPGKTTTSHALNINSSAPSTTHMLSTTSS
                     TQSTSGDTRHTTAGRTQGSTPATTQTSLTPLSQNMSTVSTPITATHKFSTLPQSQHTG
                     SKGTSSNSQTNTTPDVTISTPSATSRDQIQTETSSQRTISPGETTTSHASSLSSSGPS
                     TTNMLTTTSYTQSTSVGTGHTTAERTQGSTPATTQVLPSSQNMSTVSTPITSTQNLST
                     LLQSQHTGSMGTSSNPQTTTKPVMTTSTPSATSRDQIQTETSFQRTISSGETTTSHAP
                     SMSSSAPSSTHMLSTASSTQITSVDTRHTTAITTQGSTPATTQVSPSSQNMSTVSAPI
                     TSIQILSTLPQSQHTGSNRTSTNPQTTTIPEVTTSTPSATSHDQIETETSSQRTISPG
                     ETTTSHAPNMSSSAPSTTHMLSSTSSTQITSVDTGHTSAGRTQGSTPATTQVSPSSQN
                     MSTVSAPITSTHILSTLPKSQHTGSKGTSSNPQTTITPVVTTSTPSASSRDQIQTETS
                     FQRTISPGETTTSHAPSMSSSAPSSTHMLSTASSTQITSVDTRHTTAITTQGSTPATT
                     QVSPSSQNMSTVSAPITSIQMLSTLPQSQHTESKSTSTNPQTTTTPEVTTSNPSATSH
                     DQIETETSSQRTISPGETTTSYAPIMSSSAPSTTHMLSTTSSTQSTSVDTRNTTTLTT
                     QGSTPATTQVSPSSKNMSTVSTPITSTHKLSTLPQSQHTGSNGTSSNPQTTTTPEVTT
                     STPSATTRDQIQTESSSQRTIYPGETTTSHASIMSSSAQSSTHMLSTAYSTQITSVDT
                     RHTTAITTQGSTPATTQVSPSSQNMSTVSAPITSTQILSTFPQSQHTGRKGTSTKPQT
                     TTTPVVTTSNPSATSPDQFQTETSSQRTISPGETTTSHAPIMSSSPPSSTHMLSTASS
                     TEITSVDTRHTTAIMTQGSTPATTQVSPSSQNMSTVSAPITSTHILSTLSQSQHTGSK
                     GTSSNPQTTTTPVVTTSTPSASSRDQIQTETSSQRTISPGKTTTSHVPNMNSSAPSTT
                     HILSTTSSIQSTSGDTRHTTAVRTQGSTPATTQVSLAPSSQNMSTLSAPITSPQHFST
                     LPQNQHTGSMGTSSNPQSTTIPEVTTSTLSASSRDQVQTETSSQRTISPGETTTSHAS
                     SLSSSGPSTTNMLTRTSSTQITSGDTRHTTAIVTQGSTPATTQTTLTPSSQNMSTVST
                     PITSTHKLSPLPQSQHTENMGTSSNPQTTTTPEVTTSTPSATSYDQIQTETSFQRTIS
                     PGETTTSHAPSMSNSAPSSTHKLSTASSTEITSVDTRHTIAITTQGSTLATTQTSLTP
                     SSQNMSTVSAPITSSQILSTLRQSQHTGSKGTSSNHQTTTTPVVTTSTSSATSRDQIQ
                     TETSSLRTISPDGTTTSHASSMSSSSPNTTHLLITTSSTESTSVDTGYSTVITTHGST
                     LATTQVSLTPSSQNMSTVSMPSTSSQELTSLPQRQHTGNMETSSQPQNITPTVVTTST
                     LLSFSRGSTELQTMSWGTSSSGTINTLSTPVRNTSPASTSGILTSTLTTSGNTGYTGV
                     TRSLGVITSRVTSTSLPGKSTVVHSTPAQPLSAHSQSHQTYGTGTPSMSQTSILPDMT
                     SEKRVASSPGPTVTESFSHVSSSSGLTTKTDNDRNTAVSATSSTLTSPSPTTASRSTV
                     PLPSLLPDQGISLFPYGSEVGDQNLFARTVDFNSPIFKILIGFPLGSSLRDSFYVTDN
                     GQIIFPESDYDVFSYPNPPQRGFTGRERVAMVAPFWADADFSSSRGAIFYQEYVTFYN
                     EHHQLIREVETLINDFTSSWGYRAKWTLKVTWVNVPAYTAQESFGTNTYQAILSTDGS
                     RSYALFLYQNSGMRWDVTQEPYNRVLMGFSSGDGYFENSPLTFRPAMEKYRPDRFLNS
                     KLGIRGLQVYRLHREERPNYRLKCLQWLESQPQQPSWGWSSVSCPCSWQQGQRDFRFR
                     PINPGWWDRQLCSFSSGRGGVCCSYGAWGEFREGWRMHSPWQFDEEQEAQNWCCQWND
                     KPSFCVWYQLRRPRVSCAGYRPPRPAWTFGDPHITTLDNANFTFNGLGDFLLVQAQDR
                     NSSFLLEGRTAQTGTAKATNFIAFAAQYNTSSLKSPITVQWFLEPSDKIRVVYNNQTV
                     AFNTRDTEVLPIFNTTGVLLTQNGSQVSANFDGTVTISVIARSNILHASSSLSEEYRN
                     HTEGLLGVWNDNPEDDFRMPNGSTIPSNSSEETLFYYGMTWHVNGTGLLGIRADPLPT
                     KFTPIFLSQLLNQSASGEDLASGCKGDRKCMFDILATGNRTIGQSTNSILNEFQHMND
                     TLNQYPPSINCSSKIQAYKGQTVTTEITSNSKDATLSLSKKCSGFKLFENGSLQWTPT
                     SPEACTLEILARDVRTNLSWVLQPKTVACFCSKEEQCLYNETSKEGNSSLEVTSCKCD
                     GDTFGRLCERSKDPCDEPCFPNVNCIPGKGCEACPPNTTGDGRHCAALEDSCPNRSCP
                     MNYCYNNGHCDISEAPGCQPTCTCPPAFTDNRCFLAGNSFTPTISMELPLRTIVLSLR
                     EDENASAADVNASVANILENLDMRAFFSNSLVELIRTSPGAQPSSKSIHHWKVTSHFK
                     YRPRGPLIHYLNNQLIGAVMEAFLLQARQERQKRSGEARKDVHFFPISRADVQDQMAL
                     NLSMLEEYFTCDGYKGYHLVYSPQDGVTCVSPCSEGYCHNGGQCKHLPDGPQCSCASF
                     TIYSSSGEHCEHLSVKLGAFYGILFGTLGALLLLGILAFMIFHFCGCSKNKFSYPLDS
                     EL"
     exon            183..8666
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            8667..8795
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            8796..8929
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            8930..9094
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            9095..9250
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            9251..9381
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            9382..9470
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            9471..9650
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            9651..9767
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            9768..9887
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            9888..10099
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            10100..10184
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            10185..10352
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            10353..10454
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            10455..10688
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            10689..10826
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            10827..11008
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            11009..11168
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            11169..11342
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            11343..11416
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            11417..11487
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            11488..11723
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            11724..11886
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
     exon            11887..12452
                     /gene="Muc4"
                     /gene_synonym="4933405I11Rik; Asgp"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acctgtttgg ggccagcagc cagtggggac aggctcgcct ctgctcctcc tgctgccacc
       61 tcaagtgctg tggggattcc tgagggagcc tgagggactc tctggagcca tgagagggcc
      121 tcactggaga gttccctggc tgtgtctgag ctgcctgtat tcttgcctcc tcttgctacc
      181 tgatgctcta gcaacaacat caacacagac acccatgtcc ctttccagca gcaccagaac
      241 atcccaaatg agctcacaag catcgacctc atcaacatct tctgacagaa ggacaagtaa
      301 aacagaacaa acatcaacaa gggatactcc aagttccata acaacagttt ctcagagcca
      361 tcatactaca agcatggaga ccagcaaacc tcaaaccacc acaaccaccg aggtgaccac
      421 atcaactcct tcagcctcct cacgtgacca aatacagaca gagacaagtt ctcaaagaac
      481 aatctctcct gatggaacaa ccacctcaca tgctcccagt atcagcagct cagctccaag
      541 tacaacacac atgttaacca caacatcctc cacagaaagt acctcagtag actcaggaca
      601 cacaacagca ataacaactc aaggtttaac acctgccacc gcacaagtct cactgacacc
      661 ttcatcccag aatatgtcaa cagtgtcaac acccatcacc tcaactctta ctcagagaca
      721 acacactgga agcaagcaga ccagcagcaa atctcaagtc aacatagtca catcaactct
      781 ttcaacctct acaagtgact caactcctgc acagacaatg tctcaagtga catcatcttc
      841 agataaaaga accaagccat ctacttctgg tgtcagcagt acatcactaa ctacaacaga
      901 agtgctcacc caaacatcct ctacagactc tgctccagga aacacaactc tgaggattac
      961 tcagaactct acaacacata ccacgaaagt ctcaacaaca tctacaccac aaaaactgtc
     1021 accagtgtca acactcatca attcaagtca aaaaatgtca acccttcctc agaaccaaca
     1081 cactgaaagc atggacacca gcagacaacc tcaaaccacc acaaccatag aggtgaccac
     1141 atcaacccct tcagcctcct cacttcacca aattcagaca gagacaaatt ccccaaaaac
     1201 aatctctcct ggtgagacaa ccacctcaca tgctccgaac atgagaagct caccaccaaa
     1261 gacatcacag atactaacca caatgccctc cacaaaaagt acctcagtag acacaaaaca
     1321 aacaaaggca ataactacta aagtttctac acctgacacc acacaggtct cgatgacacc
     1381 ttcatcccag aaattgccaa cacacagcac gtctactcag gaattaacat cttcttacag
     1441 ccaacatatt caaagcaagg ggaccagcag caaatctcaa acaaccacaa acaccaaggt
     1501 gaacacatca actccttcag cctcctcacg tgacaaaatt cagacagaga caagttccca
     1561 aagaacaaac tctcctggcg agaaaaggac atcacatgct cccagcatga gcagctcagc
     1621 tccaagtaca acacacatgt tatccacaac atcctccaat caaagtacct cagtagacac
     1681 aggacaaaca acatcagtaa cagctcaagg ttctacacct gccatcacac agacctcatt
     1741 gacaccttca tcccagaata cgtcaacagt gtcaacaccc atcacctcta ctcacaaatt
     1801 gtcaaccctt tctcagagcc aacacactgg aagcaagggg accagcagca atcctcaaac
     1861 taccaccacc acagaggtga ccacatcaac tccttcagcc accacccatg accaaattca
     1921 gacagagaca agttcccaaa acacaatctc tcctggtgag acaaccacct catatgctcc
     1981 catcatgagc agctcagctc caagtacaac acatatgtta tccacaacat cctccactca
     2041 aagtacctca gtagacacaa gacacactac aacattaacg aatcaaggat ctacacctgc
     2101 caccacacag gtctcacctt catcccagaa catgtcaaca gtgtcagcac ccatcacctc
     2161 aactcaaatt ttgtcaacct ttcctcagag ccaacacact ggaagcaagg ggaccagcag
     2221 caatcctcaa actaccacca ccccagtggt gaccacatca aatccttcag ccacctcacg
     2281 tgaccaaatt cagacagaga caagttccca aagaacaatc tctcctggtg agacaaccac
     2341 ctcatatgct tccatcatga gcagctcagc tccaagtaca acacacatgt taaccacaac
     2401 atcctccact caaagtacct cagtagacac aagacacaca atagcagtaa ggactcaagg
     2461 ttctacacct gccaccacac aggtctcacc ttcatcccag aacatgtcaa cagtgtcaac
     2521 acccatcacc actactcaca aattgtcaac cctttctcag agccaacaca ctggaagcaa
     2581 ggggaccagc agcaatcctc aaactaccac aacgccagtg atgaccacat caactccttc
     2641 agccaccacc catgaccaaa ttcagacaga gacaagttct caaagaacaa tctctcatgg
     2701 tgagacaacc acctcatatg ctcccatcat gagcagctca gctccaagta caacacacat
     2761 gttatcaaca gcatcctcta ctcaaattac ctcagtagac acaagacaca caacagcaat
     2821 aacaactcaa ggttctacac ctgccaccac acaggtctca ccttcatccc agaacatgtc
     2881 aacagtgtca gcacccatca cctcaattca aattttgtca acccttcttc agagccaaca
     2941 cactggaagc aaggggacca gcaccaagcc tcaaactacc accaccccag tggtgaccac
     3001 atcaaatcct tcagccacct tccctgacca atttcagaca gagacaagtt cccaaagaaa
     3061 aatctctcct ggtgagacaa ccacctcaca tgctcccatc atgagcagct cagctccaag
     3121 tacaacacac atgttatcca caacatcttc cactcaaagt acctcagtag atacaagaca
     3181 cactacaaca gtaacgactc aaggttctac acctgccacc acacaggtct taccttcatc
     3241 ccagaacatg tcaacagtgt cagcacccat cacctcaact caaattttgt caacccttcc
     3301 tcagagccaa cacactggaa gcaaggggac cagcaccaat cctcaaacta ccaccaatgc
     3361 agaggtgacc acatcaactc cttcagccac ctcacatgac caaattgaga cagaaacaag
     3421 ttcccaaaga acaatctctc ctggtgagac aaccacctca aatgcttcca acttgagcag
     3481 ttcagctcca agtacaacac acatgttaac cacaacatcc tccactcaaa gtacctcagt
     3541 agacacagga tacacaacag cagtaaggac tcaaggttct acacctgcca ccacacaggt
     3601 ctcaccttca tcccagaaca tgtcaacagt gtcagcaccc atcacctcaa ctcaaatttt
     3661 gtcaaccctt cctcagagcc aacacactgg aagcaagggg accagcagca atcctcaaac
     3721 taccacaagc ccagtggtaa ccacatcaac tccttcaggc acctcaggtg accaaattca
     3781 gacagagaca agttcccaaa gaacaatctc tcccggcaag acaaccactt cacatgctct
     3841 caacataaac agctcagctc caagtacaac acacatgtta tccacaacat cctccactca
     3901 aagtacctca ggagacacaa gacacacaac agcaggaagg actcaaggtt ctacacctgc
     3961 caccacacag acctcattga cacctttgtc ccagaatatg tcaacagtgt caacacccat
     4021 cactgctact cacaaattct caacccttcc tcagagccaa cacactggaa gcaaggggac
     4081 cagcagcaat tctcaaacta acaccacccc agatgtgacc atatcaactc cttcagccac
     4141 ctcacgtgac caaattcaga cagagacaag ttcccaaaga acaatctctc ctggggagac
     4201 aaccacctca catgcttcca gcttgagcag ttcaggacca agtacaacaa acatgttaac
     4261 cacaacatcc tacactcaaa gtacctcagt aggcacagga cacacaacag cagaaagaac
     4321 tcaaggttct acacctgcca ccacacaggt cttaccttca tcccagaaca tgtcaacagt
     4381 gtcaacaccc atcacatcta ctcaaaattt gtcaaccctt cttcagagcc aacacactgg
     4441 aagcatgggg actagtagca atcctcaaac taccaccaag ccagtgatga ccacatcaac
     4501 tccttcagcc acctcacgtg accaaattca gacagagaca agtttccaaa gaacaatctc
     4561 ttctggtgag acaaccacct cacatgctcc cagcatgagc agctcagctc caagttcaac
     4621 acacatgtta tcaacagcat cctctactca aattacctca gtagacacaa gacacacaac
     4681 agcaataaca actcaaggtt ctacacctgc caccacacag gtctcacctt catcacagaa
     4741 tatgtcaaca gtgtcagcac ccatcacctc aattcaaatt ttgtcaaccc ttcctcagag
     4801 ccaacacact ggaagcaata ggaccagcac caatcctcaa actaccacca tcccagaggt
     4861 gaccacatca actccttcag ccacctcaca tgaccaaatt gagacagaaa caagttccca
     4921 aagaacaatc tctcctggtg agacaaccac ctcacatgct cccaacatga gtagttcagc
     4981 tccaagtaca acacacatgt tatcctcaac atcctccact caaattacct cagtagacac
     5041 aggacacaca tcagcaggaa ggactcaagg ttctacacct gccaccacac aggtctcacc
     5101 ttcatcccag aacatgtcaa cagtgtcagc acccatcacc tcaactcata ttttgtcaac
     5161 ccttccaaag agccaacaca ctggaagcaa ggggaccagc agcaatcctc aaactaccat
     5221 aaccccagtg gtaaccacat caactccttc agcctcctcc cgtgaccaaa ttcagacaga
     5281 gactagtttc caaagaacaa tctctcctgg cgagacaacc acctcacatg ctcccagcat
     5341 gagcagctca gctccaagtt caacacacat gttatcaaca gcatcctcta ctcaaattac
     5401 ctcagtagac acaagacaca caacagcaat aacaactcaa ggttctacac ctgccaccac
     5461 acaggtctca ccttcatccc agaacatgtc aacagtgtca gcacccatca cctcaattca
     5521 aatgttgtca acccttcctc agagccaaca cactgaaagc aagagtacca gcaccaatcc
     5581 tcaaactacc accaccccag aggtgaccac atcaaatcct tcagccacct cacatgacca
     5641 aattgagaca gaaacaagtt cccaaagaac aatctctcct ggtgagacaa ccacctcata
     5701 tgctcccatc atgagcagct cagctccaag tacaacacac atgttatcca caacatcctc
     5761 cactcaaagt acctcagtag acacaagaaa cactacaaca ttaacgactc aaggatctac
     5821 acctgccacc acacaggtct caccttcatc caagaacatg tcaacagtgt caacaccaat
     5881 cacctctact cacaaattgt caacccttcc tcagagccaa cacactggaa gcaatgggac
     5941 cagcagcaat cctcaaacta ccaccacccc agaggtgacc acatcaactc cttcggccac
     6001 cacccgtgac caaattcaga cagagtcaag ttcccaaaga acaatctatc ctggtgagac
     6061 aaccacctca catgcttcca tcatgagcag ctcagctcaa agttcaacac acatgttatc
     6121 aacagcatac tccactcaaa ttacctcagt agacacaaga cacacaacag caataacaac
     6181 tcaaggttct acacctgcca ccacacaggt ctcaccttca tcccagaaca tgtcaacagt
     6241 gtcagcaccc atcacctcaa ctcaaatttt gtcaaccttt cctcagagcc aacacactgg
     6301 aaggaagggg accagcacca agcctcaaac taccacaacc ccagtggtga ccacatcaaa
     6361 tccttcagcc acctcccctg accaatttca gacagagaca agttcccaaa gaacaatctc
     6421 tcctggtgag acaaccacct cacatgctcc catcatgagc agctcacctc caagttcaac
     6481 acacatgtta tcaacagcat cctccactga aattacctca gtagacacaa gacacacaac
     6541 agcaataatg actcaaggtt ctacacctgc caccacacag gtctcacctt catcccagaa
     6601 catgtcaaca gtgtcagcac ccatcacctc aactcatatt ttgtcaaccc tttctcagag
     6661 ccaacacact ggaagcaagg ggaccagcag caatcctcaa actaccacaa ccccagtggt
     6721 aaccacatca actccttcag cctcctcacg tgaccaaatt cagacagaga caagttccca
     6781 aagaacaatc tctcccggca agacaaccac ctcacatgtt cccaacatga acagctcagc
     6841 tccaagtaca acacacatct tatccacaac atcctccatt caaagtacct caggagatac
     6901 aagacacaca acagcagtaa ggactcaagg ttctacacct gccaccacac aggtctcact
     6961 ggcaccttca tcccagaaca tgtcaacact gtcagcaccc atcacatcac ctcaacattt
     7021 ttcaaccctt cctcagaacc aacacactgg aagcatgggg accagcagca atcctcaaag
     7081 taccaccatc ccagaggtga ccacatcaac tctttcagct tcctcacgtg accaagttca
     7141 gacagagaca agttcccaaa gaacaatctc tcctggtgag acaaccacct cacatgcttc
     7201 cagcttgagc agttcaggac caagtacaac aaacatgtta accagaacat cctccactca
     7261 aattacctca ggagacacaa gacacacaac agcaatagtg actcaaggtt ctacacctgc
     7321 caccacacag accacattga caccttcgtc ccagaatatg tcaacagtgt caacacccat
     7381 cacttctact cacaaattgt caccccttcc tcagagccaa cacactgaaa acatggggac
     7441 cagcagcaat cctcaaacta ccaccacccc agaagtgacc acatcaactc cttcagccac
     7501 ctcctatgac caaattcaga cagagacaag tttccaaaga acaatctctc ctggtgagac
     7561 aaccacttca catgctccca gcatgagcaa ctcagctcca agttcaacac acaagttatc
     7621 aacagcatcc tccactgaaa ttacctcagt agacacaaga cacacaatag caataacgac
     7681 tcaaggttct acacttgcca ccacacagac ctcactgaca ccttcatccc agaacatgtc
     7741 aacagtgtca gcacccatca cctcatctca aattttgtca actcttcgtc agagccaaca
     7801 cactggaagc aaggggacca gcagcaatca tcagactacc actacccctg tggtgaccac
     7861 atcaacttct tcagccacct cacgtgacca aattcagaca gagacaagtt ccctaagaac
     7921 aatctctcct gatggaacga ccacctcaca tgcttccagc atgagcagtt catctccaaa
     7981 tacaacacac ttgttaatca ctacatcctc cacagaaagt acctcagtag acacaggata
     8041 ctcaacagta ataacgactc atggttctac acttgccacc acccaggtct cattgacacc
     8101 ttcatcccag aatatgtcaa cagtgtcaat gcccagcact tcttcacagg aattaacatc
     8161 acttcctcag agacaacaca ctggaaacat ggagaccagc agccaacctc agaatatcac
     8221 ccctactgtg gtgaccacat caactctttt atcattctca aggggatcca ctgaattgca
     8281 aaccatgtct tggggaacgt cctcctcagg gacaataaat accctatcta ctcccgtcag
     8341 gaatacctca cctgcatcca catcaggcat cctcaccagc acattgacca cctcaggaaa
     8401 cacagggtac acaggtgtaa ctagaagcct cggtgtgatc acatcaagag tcacaagcac
     8461 aagtctacct ggaaagtcca cagtagtgca tagcacccct gctcagccat tgtctgcaca
     8521 ttctcagagt caccagacgt atggcacggg gacacccagc atgtctcaaa ccagcatcct
     8581 cccagacatg acctcagaaa agcgtgttgc ctcttcacct ggacccactg tgacagagtc
     8641 cttttctcat gtgtcatcct cctcaggatt gactacgaag acagacaatg acagaaacac
     8701 agcagtctca gccacctcct cgacccttac tagccccagc ccaaccacag cctccaggtc
     8761 aacagtgccc ctgccttctc ttttaccaga tcagggcatt tccctcttcc cctacgggtc
     8821 tgaagtcgga gaccagaatt tgtttgccag gactgtggat tttaactcac caatcttcaa
     8881 gatcctgatt ggctttcccc tcggttcctc cctgcgggat tccttctacg ttacagataa
     8941 tggtcagatc attttcccag agtcagacta tgacgtcttc tcctacccca acccacctca
     9001 aagaggcttc accggaaggg agcgcgtggc catggtggcc ccgttctggg ctgatgctga
     9061 tttctctagc tccaggggag ccatatttta ccaggaatat gtcacatttt acaatgaaca
     9121 ccatcagcta atccgggaag tggagacttt gattaatgat ttcacaagta gctggggcta
     9181 cagagccaag tggacactga aggtcacctg ggtcaatgtt cctgcctata ctgcccaaga
     9241 gagctttggg accaatacct accaagccat cctctccaca gatgggagca ggtcctatgc
     9301 cctgtttctc taccagaaca gtggcatgag gtgggatgtg acccaggaac catacaaccg
     9361 ggtgctcatg ggcttctcca gtggagatgg gtattttgaa aacagcccgc tgacattccg
     9421 gccagcaatg gagaagtacc gtccagatcg attcctgaat tccaaattag gcatccgggg
     9481 actgcaggtc tacaggctgc acagggagga gcggcccaac taccggctca aatgtctgca
     9541 gtggctggag agtcagcctc agcagcccag ctggggctgg agctcagttt cctgcccttg
     9601 ctcctggcag cagggacaac gggactttcg gttcaggccc atcaacccag gttggtggga
     9661 caggcagctg tgcagcttct cctccgggcg agggggtgtg tgctgcagct atggtgcctg
     9721 gggagagttt cgagaaggct ggagaatgca cagtccttgg cagtttgatg aggagcagga
     9781 ggcacagaac tggtgctgcc aatggaacga caagccctcg ttctgtgtct ggtaccagct
     9841 taggcggccc cgtgtcagct gtgctgggta caggccccca cggcccgctt ggacatttgg
     9901 tgatccccac atcaccactt tggataatgc caacttcacc ttcaacgggc tgggagactt
     9961 tctgctggtc caggcccagg acagaaattc ttccttcctg ctggaaggcc gcactgccca
    10021 gactggcact gccaaggcca ctaacttcat tgcctttgcg gctcaataca acaccagcag
    10081 cctgaagtct cccatcacgg ttcagtggtt tcttgagccc agtgacaaaa tccgagtcgt
    10141 atacaataac cagactgtgg cctttaacac cagagacact gaagtcttac ccatattcaa
    10201 taccaccggt gttctgctga cccaaaatgg ctcccaagtc tcagccaact ttgatgggac
    10261 agtgactatc tctgtgattg ctcgctccaa catccttcat gcctcctcca gcctatcaga
    10321 ggaatatcga aaccacacag agggccttct gggagtgtgg aatgacaatc cagaagatga
    10381 cttcaggatg cccaatggct ccaccattcc ctcaaacagc tctgaggaga cccttttcta
    10441 ctatggaatg acatggcacg tcaacggaac aggcctcctt gggataaggg cagacccgct
    10501 gcctaccaag ttcaccccca tctttctgtc tcaactgttg aatcagagtg cctcaggtga
    10561 agacttggcc tctgggtgca aaggcgacag aaagtgcatg tttgacatcc tggccacggg
    10621 aaacagaact attggacaaa gcaccaattc catccttaat gagttccaac atatgaatga
    10681 caccctcaat cagtacccac cctctatcaa ctgcagcagc aagattcaag cctacaaggg
    10741 gcagacagtg accactgaga tcaccagcaa ctctaaggat gccacactca gcctctccaa
    10801 gaaatgtagt ggctttaagc tctttgaaaa cgggagtttg cagtggacac caacatcccc
    10861 agaagcgtgt accctggaga ttctagcgag ggatgtcagg actaacttgt catgggtact
    10921 ccagcccaag accgtggctt gcttctgcag taaggaggag cagtgtttgt acaatgaaac
    10981 cagcaaagag ggcaattctt cccttgaggt gaccagctgc aagtgtgatg gggacacctt
    11041 tggccgcttg tgtgaacgct ccaaggaccc gtgtgatgag ccatgcttcc ctaacgtgaa
    11101 ctgcattcct gggaagggct gtgaggcctg ccctccaaac acaactggag atggacgtca
    11161 ttgtgcagct ctggaggact cctgcccaaa ccgttcctgt cccatgaatt actgctataa
    11221 caatggccat tgcgacatct ctgaggctcc aggctgccag cccacttgca cctgtccccc
    11281 tgccttcact gataaccgct gcttcctggc tgggaacagt ttcactccca ccatctctat
    11341 ggagcttccc ttaaggacca tcgtgctctc tctcagggag gatgaaaatg cctccgccgc
    11401 tgacgtcaac gcctcggtgg cgaacatact agagaacctg gacatgcggg cttttttctc
    11461 caacagctta gtggaactga tacgaacctc tcccggagca cagccctcga gcaagtccat
    11521 tcatcactgg aaggtcacct cccacttcaa gtaccgtccc aggggcccgc tcatccacta
    11581 tctgaacaac cagctgatag gcgccgtgat ggaggccttc ctcctgcagg ctcggcagga
    11641 gaggcagaag aggagtggag aagccaggaa ggacgtccac ttcttcccca tctcgagggc
    11701 agatgtccag gaccagatgg ctctgaacct aagtatgctg gaggagtact tcacatgtga
    11761 tggctacaaa ggctaccacc tggtctacag cccacaggat ggtgtcacct gtgtgtcccc
    11821 atgtagtgag ggctactgtc acaatggagg ccaatgcaag cacctgccag atgggcccca
    11881 atgcagctgc gcatccttca ccatctactc atcctcgggc gaacactgtg agcacctaag
    11941 tgtgaaactt ggggcattct acgggatcct cttcggaacc ctgggcgccc tcttgctact
    12001 ggggatctta gcatttatga tctttcactt ctgcggctgc tccaagaaca agttctccta
    12061 ccctttggac tcagaattgt aaggccctgt cccagatggg cagctgcacc taggacctcg
    12121 ggacccatcc ctcagtctgc ccctgctctg tgggactgga aagggcctgc ggcatgaaga
    12181 tgatttgaga ctcttcaaga gtgaataaac cagactctac ctgtttagta gactgggggt
    12241 acaggaaaag gccatggtgg ccaaatatac agatggatgg gttcctacac agatatatca
    12301 ggaaggagga tattgcttat atatgaatgc agactgtgtg tgcatacaca cacacacaca
    12361 ccagagttaa tggatgtgac tggctttaca tttaccaaga tgtttttaca tataacacag
    12421 gcttacttct cattaaaatc tatttaaata aa
//
