LOCUS       NM_001308475            4477 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus 5-methyltetrahydrofolate-homocysteine
            methyltransferase reductase (Mtrr), transcript variant 1, mRNA.
ACCESSION   NM_001308475 XM_006517180
VERSION     NM_001308475.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Senner,C.E., Dong,Z., Prater,M., Branco,M.R. and Watson,E.D.
  TITLE     One-carbon metabolism is required for epigenetic stability in the
            mouse placenta
  JOURNAL   Front Cell Dev Biol 11, 1209928 (2023)
   PUBMED   37440923
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4477)
  AUTHORS   Wilkinson,A.L., Menelaou,K., Rakoczy,J., Tan,X.S. and Watson,E.D.
  TITLE     Disruption of Folate Metabolism Causes Poor Alignment and Spacing
            of Mouse Conceptuses for Multiple Generations
  JOURNAL   Front Cell Dev Biol 9, 723978 (2021)
   PUBMED   34957089
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4477)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4477)
  AUTHORS   Bertozzi,T.M., Becker,J.L., Blake,G.E.T., Bansal,A., Nguyen,D.K.,
            Fernandez-Twinn,D.S., Ozanne,S.E., Bartolomei,M.S., Simmons,R.A.,
            Watson,E.D. and Ferguson-Smith,A.C.
  TITLE     Variably methylated retrotransposons are refractory to a range of
            environmental perturbations
  JOURNAL   Nat Genet 53 (8), 1233-1242 (2021)
   PUBMED   34326545
REFERENCE   6  (bases 1 to 4477)
  AUTHORS   Deng,L., Elmore,C.L., Lawrance,A.K., Matthews,R.G. and Rozen,R.
  TITLE     Methionine synthase reductase deficiency results in adverse
            reproductive outcomes and congenital heart defects in mice
  JOURNAL   Mol Genet Metab 94 (3), 336-342 (2008)
   PUBMED   18413293
  REMARK    GeneRIF: Mtrr deficiency adversely impacts reproductive outcomes
            and cardiac development in mice.
REFERENCE   7  (bases 1 to 4477)
  AUTHORS   Froese,D.S., Wu,X., Zhang,J., Dumas,R., Schoel,W.M., Amrein,M. and
            Gravel,R.A.
  TITLE     Restricted role for methionine synthase reductase defined by
            subcellular localization
  JOURNAL   Mol Genet Metab 94 (1), 68-77 (2008)
   PUBMED   18221906
REFERENCE   8  (bases 1 to 4477)
  AUTHORS   Elmore,C.L., Wu,X., Leclerc,D., Watson,E.D., Bottiglieri,T.,
            Krupenko,N.I., Krupenko,S.A., Cross,J.C., Rozen,R., Gravel,R.A. and
            Matthews,R.G.
  TITLE     Metabolic derangement of methionine and folate metabolism in mice
            deficient in methionine synthase reductase
  JOURNAL   Mol Genet Metab 91 (1), 85-97 (2007)
   PUBMED   17369066
REFERENCE   9  (bases 1 to 4477)
  AUTHORS   Juriloff,D.M., Harris,M.J. and Dewell,S.L.
  TITLE     A digenic cause of cleft lip in A-strain mice and definition of
            candidate genes for the two loci
  JOURNAL   Birth Defects Res A Clin Mol Teratol 70 (8), 509-518 (2004)
   PUBMED   15329828
REFERENCE   10 (bases 1 to 4477)
  AUTHORS   Leclerc,D., Wilson,A., Dumas,R., Gafuik,C., Song,D., Watkins,D.,
            Heng,H.H., Rommens,J.M., Scherer,S.W., Rosenblatt,D.S. and
            Gravel,R.A.
  TITLE     Cloning and mapping of a cDNA for methionine synthase reductase, a
            flavoprotein defective in patients with homocystinuria
  JOURNAL   Proc Natl Acad Sci U S A 95 (6), 3059-3064 (1998)
   PUBMED   9501215
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT033781.12.
            
            On Jan 11, 2023 this sequence version replaced NM_001308475.1.
            
            Summary: Methionine is an essential amino acid required for protein
            synthesis and one-carbon metabolism. Its synthesis is catalyzed by
            the enzyme methionine synthase. Methionine synthase eventually
            becomes inactive due to the oxidation of its cob(I)alamin cofactor.
            The protein encoded by this gene regenerates a functional
            methionine synthase via reductive methylation. It is a member of
            the ferredoxin-NADP(+) reductase (FNR) family of electron
            transferases. Mutations in a similar gene in human have been
            associated with cblE complementation type
            homocystinuria-megaloblastic anemia and susceptibility to
            folate-sensitive neural tube defects. Alternative splicing of this
            gene results in multiple transcript variants. [provided by RefSeq,
            May 2015].
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Both variants 1 and 2 encode the same protein.
            
            Sequence Note:.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13861889.38704.1,
                                           SRR17784647.1074678.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-840               CT033781.12        156499-157338       c
            841-1005            CT033781.12        155754-155918       c
            1006-1159           CT033781.12        154746-154899       c
            1160-1277           CT033781.12        152791-152908       c
            1278-1650           CT033781.12        150221-150593       c
            1651-1773           CT033781.12        147781-147903       c
            1774-1927           CT033781.12        146273-146426       c
            1928-2016           CT033781.12        145207-145295       c
            2017-2197           CT033781.12        143975-144155       c
            2198-2240           CT033781.12        142145-142187       c
            2241-2427           CT033781.12        141271-141457       c
            2428-2546           CT033781.12        140313-140431       c
            2547-2639           CT033781.12        139803-139895       c
            2640-2822           CT033781.12        139523-139705       c
            2823-4477           CT033781.12        135981-137635       c
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 35.54 cM"
     gene            1..4477
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /note="5-methyltetrahydrofolate-homocysteine
                     methyltransferase reductase"
                     /db_xref="GeneID:210009"
                     /db_xref="MGI:MGI:1891037"
     exon            1..840
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            841..1005
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     CDS             877..2967
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /EC_number="1.16.1.8"
                     /note="aqCbl reductase; aquacobalamin reductase"
                     /codon_start=1
                     /product="methionine synthase reductase"
                     /protein_id="NP_001295404.1"
                     /db_xref="CCDS:CCDS26620.1"
                     /db_xref="GeneID:210009"
                     /db_xref="MGI:MGI:1891037"
                     /translation="MRRFLLLYATQRGQAKAIAEEISEQAVSHGFSADLHCISESEKY
                     DLKTETGPLVMVVSTTGTGDPPDTARKFVKEIHNKTLPTDYFAHLQYGLLGLGDSEYT
                     YFCNGGKVIDKRLQELGAQRFYDTGHADDCVGLELVVEPWIDGLWAALTKHFKSLGGQ
                     ENMSDTLSRASDAPLSTAMKPELLHIQSQVELLRLEDMGERDSELREQNETNRGQQGR
                     IEDFDSSLVHSVPPLSQSSLSIPAVPPEYLEVHLQESLGQEENQASVPSGDPSFQVPI
                     SKAIRLTTNDAVKSTLLLELDISKIEFSHQPGDSFNVTCPNSDREVEELLQRLQLADK
                     RAHRVILKIKTDTKKKGAALPAHVPEGRSLQFILTWCLEIRAVPKKAFLRALAEHTSS
                     ATEKRRLQELCSKQGAADYNRFIRDASVCLLDLLLTFPSCQPPLSLLLEHLPKLQPRP
                     YSCASSSLRHPDKLHFVFNIVEFPPSTTAASLRKGVCTGWLATLVAPFLQPNTDVSNV
                     DSGDALAPEIRISPRATNAFHLPEDPSAPIIMVGPGTGVAPFVGFLQHREKLQEQHPD
                     GKFGAMWLFFGCRHKDRDYLFREELRHFLKTGVLTHLKVSFSRDAAPDGEEAPAKYVQ
                     DNLQRHSQQVARTLLQENGYVYVCGDAKNMAKDVNDTLIGIISNEAGVDKLEAMKTLA
                     TLKQEKRYLQDIWS"
     exon            1006..1159
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            1160..1277
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            1278..1650
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            1651..1773
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            1774..1927
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            1928..2016
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2197
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            2198..2240
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            2241..2427
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            2428..2546
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            2547..2639
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            2640..2822
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     exon            2823..4477
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3956..3961
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /note="hexamer: AATAAA"
     polyA_site      3974
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /note="major polyA site"
     regulatory      4446..4451
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
                     /note="hexamer: ATTAAA"
     polyA_site      4477
                     /gene="Mtrr"
                     /gene_synonym="4732420G08; MSR"
ORIGIN      
        1 gcgtgcgcag tgctcccgga ctgtgcctgt cggaggagaa cggggttccg cgcgggaatt
       61 cgcgcctgga ggtattcggc gggggacggc gggagcgacg gaagcggtgg gggagccggg
      121 actcgcgcct gcgcagtggg gatgagccgc tgagttttcc ggaccgcggt ggggttcacg
      181 gggagcactt gggaggcgga ccgcggtggg gttcacgggg agcacttggg aggagtcggc
      241 tccgccacct ttcccaaaac ctcgtgagct cgcacgtgtc acccattggt cctgtcgagt
      301 ctgtggcagt gcttcccgat gaacctttga agggtttgtg tcctagctcc ccagcggata
      361 agccacaagt gcttagaaac taactagagt tgattcactc aggaaaagat aattttatat
      421 aggccggcta ccactcagcg ttagccactg gatagaatgg cggagtgtgg aatcccagag
      481 ccctttatct tctttttctc ttggttatct ccggggtaag gcatgaatta gaaataccgt
      541 ctctgtgtaa agtccatgta cgctgactac tgatagatgc aatgatgcca tctttgatga
      601 cgaccagtgc tttgattagg atctagcagg aaacacgaac tccccagttt cacgtgaaca
      661 ttgtacagtt acagttttgg ttttttaaag attacttatt tattttatat atatgagtat
      721 actgtggctg tactgatggt tgtgaaccat catgtcgttt ctgggacttt gaattcagaa
      781 cctttacttg ctccggagga tcctgctcgc tccagcctaa aggtttattt attattatat
      841 tctcactgtt agagttgcag ctgatgacct gaggtgatga gaaggttttt gttactttat
      901 gctacacagc gaggacaggc aaaggccata gcagaagaaa taagtgagca ggctgtgtca
      961 cacgggtttt ccgcagatct tcactgtatc agtgagtcag aaaagtatga tctgaaaaca
     1021 gaaactggcc ctctggtgat ggttgtatct accacgggta caggagaccc acctgacaca
     1081 gcccggaagt ttgttaaaga aatacacaac aagacactcc ccacggacta ttttgctcac
     1141 ctgcaatatg gattattggg cttgggtgac tcagaataca catacttctg caatggaggg
     1201 aaagtaattg ataaacgact tcaggagctt ggagcccagc gtttctacga caccggacat
     1261 gccgatgact gtgtcggttt agagcttgtg gtagagccgt ggattgatgg actctgggct
     1321 gctctcacaa agcactttaa gtcactcgga ggacaagaaa acatgagtga cacactctca
     1381 cgagcatcag atgccccctt gagcactgcc atgaagccgg agttgttgca catccagtca
     1441 caagttgaac tgctgagatt agaggacatg ggggaaaggg attctgagct tcgggaacaa
     1501 aatgagacaa acaggggtca gcagggtcgg atcgaagact ttgactcctc gcttgtgcac
     1561 tcggtccccc cactctccca gagctctcta agtattcctg ctgtgccccc agagtatttg
     1621 gaggtccatc ttcaagagtc tcttggccag gaggaaaacc aagcatctgt gccttcaggg
     1681 gatccaagtt ttcaagttcc aatttcaaaa gccattcggc tgactacaaa tgatgccgta
     1741 aagagcactc tgctgttgga actggacatt tcaaaaatag agttttccca tcaacctgga
     1801 gactccttca atgtgacctg tccaaacagt gatcgtgagg tagaagaact gctccaaagg
     1861 ctgcagctgg ctgataaacg agctcaccgt gtcatcttga aaattaagac ggacactaag
     1921 aagaaaggag ctgccctgcc tgcgcatgtg cctgaggggc gttctctcca gttcatcctc
     1981 acctggtgtc ttgaaatacg agcagttcct aaaaaggcct ttttgcgggc ccttgccgag
     2041 cacaccagca gtgccactga aaagcggagg ctgcaggagc tgtgcagtaa acaaggagcc
     2101 gctgattaca accgcttcat ccgagatgcc agcgtctgcc tgctggacct cctgctcacc
     2161 ttcccgtcct gccagcctcc gctcagccta ctgctggagc accttcctaa acttcagcct
     2221 cgaccgtact catgtgcaag ctccagctta cgtcacccag acaagcttca cttcgtgttt
     2281 aacatcgtgg agttcccacc tagcaccact gcagcctccc tgaggaaggg agtgtgcacg
     2341 ggctggctag ccacattggt tgctccattc cttcagccaa acacagacgt ttcgaatgta
     2401 gacagtggtg acgctctggc tcctgagata cgtatctctc ctcgggcaac aaacgctttc
     2461 cacttaccag aggacccgtc ggcccccatc ataatggtgg gtccaggaac tggcgtagcc
     2521 ccctttgttg gcttcctcca gcacagagaa aaacttcaag aacaacaccc agatgggaaa
     2581 tttggagcta tgtggctgtt ctttggctgc agacacaagg acagagacta cttgttcagg
     2641 gaggagctca ggcatttcct caagactggg gtcttgactc atctgaaggt ctcgttctca
     2701 agagacgctg cccctgacgg ggaggaggcc ccagcaaagt atgtgcaaga caacctccag
     2761 cggcacagcc agcaggtggc caggaccctc cttcaggaga atggctacgt ttacgtgtgt
     2821 ggagacgcca agaatatggc caaggacgtg aacgacaccc tcataggaat cataagcaat
     2881 gaagctggtg ttgacaaact agaagcaatg aagacgctgg caacgttaaa gcaagagaaa
     2941 cgatatctgc aggacatctg gtcctaaact ggaacagaga cgcttggaat ttgtttttgt
     3001 ttttgtttgt ttgtttgttt tttcttgaag tgccaaaggt caattttaca gaagatgagt
     3061 ttaaaaatgt ttggacattt cttggaggtg gctgtgtggg gaacggcttg cttacaggta
     3121 cagcaggtac aaccgcctga cagtctcaca ggttgtatcc cactccttac ctgtggcccc
     3181 acccagcaca tcactacgtg gaggggaaag aggcagctcc cacctggcac ccattgcaca
     3241 taggctcccc aggaggacag atcatcatcc acggctcacc tgtgcctggg cacatcttgg
     3301 agagcttgtg gacatactat ctttcaatga agtatattca aaggatattt atattggtga
     3361 tatttaaatt aacacagaca tgcaaagcat tcttttgcat gtctttattt aattgtggtt
     3421 ttattatcaa aattatattt ctgataaaat attttaattt tgaggtaatc cttacaaaat
     3481 gaaggttttc atgcaactag aaatatattt tgaaatatat ttgatatagt actctgccat
     3541 tttcatcatg ttaactttca gtgtcaccat cttacagctt aggggcattt gcattcccct
     3601 gacaaaggta tcatgtgtac agctcagtag tgtgccctgt ccttctcatg gactccattc
     3661 atgtctgctc ctcactaaca gacttgggtt ttcccaatac ctgactatac ttttgtgatt
     3721 cagttcagct taaagaacgc cagttaggcc gggcgtggtg gcgcacgtct ttaatcccag
     3781 cacttgggag gcaggcagag ataggcggat ttctgagttt gaggccagcc tggtctacag
     3841 agtgagttcg aggacaacca gggctacaca gagaaaccct gtctcgaaaa ccaaaaacaa
     3901 aaaaaaagaa ctccagttaa gacttctaat accaaattct cttgcaagtt atgaaaataa
     3961 agtatataaa aggatcctgt gctattgtgt gcgtggttgc tgagacaaaa actgggacaa
     4021 agcaacttac agagggtatt ttagctcagt tcaggtgtgg ggtccacagt gctgaggaaa
     4081 tcatagtggc agaaggagga ggtgactggt tacatcccgt ccagtcagga aggaaaagaa
     4141 aaggaaaagt gcctcgctgg cttcctcctt ttcacacact ccaatacttt aatgcatgga
     4201 acaggtctgc ctatgtgtag tgtgggtgtc cacttcagcc tagtgtagag gacctctcga
     4261 agacatgccc aggggttttt ttccagtgag gttgtagacc ctgcaaacct aactgtaaca
     4321 tagccttcac aagcatgata aaccctccct gttgtgtata gtaacggtct cattttatag
     4381 tccaccttta aaacacatct ctacctggtg aaaagtcatc acaagactcc taggtcatta
     4441 taagaattaa agatttgaaa gagtaatata atatcaa
//
