LOCUS       NM_001134717            1694 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus mitochondrial translation release factor in rescue
            (Mtrfr), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001134717
VERSION     NM_001134717.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Kogure,H., Hikawa,Y., Hagihara,M., Tochio,N., Koshiba,S., Inoue,Y.,
            Guntert,P., Kigawa,T., Yokoyama,S. and Nameki,N.
  TITLE     Solution structure and siRNA-mediated knockdown analysis of the
            mitochondrial disease-related protein C12orf65
  JOURNAL   Proteins 80 (11), 2629-2642 (2012)
   PUBMED   22821833
  REMARK    GeneRIF: Determined the solution structure of the GGQ-containing
            domain (GGQ domain) of C12orf65 from mouse by NMR spectroscopy.
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Jerome-Majewska,L.A., Achkar,T., Luo,L., Lupu,F. and Lacy,E.
  TITLE     The trafficking protein Tmed2/p24beta(1) is required for
            morphogenesis of the mouse embryo and placenta
  JOURNAL   Dev Biol 341 (1), 154-166 (2010)
   PUBMED   20178780
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK039255.1, AK140664.1, CA548248.1 and AC127339.3.
            
            On Nov 5, 2013 this sequence version replaced NM_001134717.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC054073.1, BQ951748.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-58                AK039255.1         2-59
            59-616              AK140664.1         2-559
            617-933             CA548248.1         272-588
            934-1694            AC127339.3         83554-84314
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 63.52 cM"
     gene            1..1694
                     /gene="Mtrfr"
                     /gene_synonym="2810006K23Rik"
                     /note="mitochondrial translation release factor in rescue"
                     /db_xref="GeneID:72650"
                     /db_xref="MGI:MGI:1919900"
     exon            1..141
                     /gene="Mtrfr"
                     /gene_synonym="2810006K23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            142..450
                     /gene="Mtrfr"
                     /gene_synonym="2810006K23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..714
                     /gene="Mtrfr"
                     /gene_synonym="2810006K23Rik"
                     /note="isoform a is encoded by transcript variant 1;
                     Probable peptide chain release factor C12orf65 homolog,
                     mitochondrial"
                     /codon_start=1
                     /product="mitochondrial translation release factor in
                     rescue isoform a"
                     /protein_id="NP_001128189.1"
                     /db_xref="CCDS:CCDS51647.1"
                     /db_xref="GeneID:72650"
                     /db_xref="MGI:MGI:1919900"
                     /translation="MSSRSTWALLRLPLPLIRICSGKWGLRLQEKPALLFPGMAASTV
                     QVAGRKDYPALLPLNESELEEQFVKGHGPGGQATNKTSNCVVLKHVPSGIVVKCHQTR
                     SVDQNRKIARKVLQEKVDVFYNGENSPVHKEKLEAERRKRERKKRAKETLEKKKLLKE
                     LREASQNITEKKADADGIPRGFQE"
     exon            451..1694
                     /gene="Mtrfr"
                     /gene_synonym="2810006K23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1651..1656
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtrfr"
                     /gene_synonym="2810006K23Rik"
                     /note="hexamer: AACAAA"
     regulatory      1662..1667
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtrfr"
                     /gene_synonym="2810006K23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1669
                     /gene="Mtrfr"
                     /gene_synonym="2810006K23Rik"
                     /note="major polyA site"
     polyA_site      1686
                     /gene="Mtrfr"
                     /gene_synonym="2810006K23Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcagttgatg agaatgggcc ctctggtagg ctgggagaag ccgtgggagg agtacgacgc
       61 atgcgcagac ctggaaacct tagatttccc atcctcggtc taccgtgggt ttagagtctc
      121 gccgaatgct tccggcccga ggtcctgcca caccaagcca tgtcttctag gagcacctgg
      181 gctctgctcc gcctccctct accactgatc aggatatgct ctgggaagtg ggggctcagg
      241 cttcaggaga agccagcact gctcttccca ggaatggctg ccagcacagt acaggtggca
      301 ggcaggaagg actaccctgc tctgctcccc ctgaatgaga gtgagctcga agaacagttc
      361 gtgaaaggac atggcccagg gggccaggcc accaacaaga ccagcaattg tgtagtgctc
      421 aaacacgtgc cctccggcat tgtggtcaag tgccaccaaa caagatctgt ggatcaaaac
      481 aggaagatag ctcggaaagt cctccaggag aaagtggatg ttttctacaa tggtgaaaac
      541 agccccgttc acaaagagaa gctcgaggct gagaggagaa agcgagagag gaagaaaaga
      601 gcaaaggaga ctctagaaaa gaaaaaatta ttgaaagaat tacgggaagc aagtcaaaac
      661 atcacagaga aaaaggctga tgctgatggt ataccaaggg gcttccagga atagaggtgg
      721 cagtgcctgg ctccagcatt ataacatcta aagagaacat tctagaactg gaaaaggtgg
      781 cgtatacctg cagcctcagc acttgggaga gctaaggcag gaggattgct gcaaggttca
      841 gaacaacctc acctatatag ctagttctgt agcggccttt cctatatggt gagactgtcc
      901 caaatacgta attagaggct gcagagttag cttagcagtt gagagagctt gcagttcttg
      961 cagaagactc aggtcctgtt cccagtattt cacatggggt tgctcccaac ctactgcctg
     1021 taactacagc tccaggagac ccagtgccac tgtctggcct tcacaggaaa aaatacactc
     1081 tcatacatac aaatcccttt tggggggagg gggggttgag acagggtttc tctgtatagc
     1141 cctggctgtc ctggaactca ctttgtagac caggctggcc tcgaactcag aaatccgcct
     1201 gcctctgcct cctgagtgct ggaattaaag gcatgcgcca ccacacccag ctcacacaaa
     1261 tcctcttaag aaccttttaa aaatatcatc ctgatggact taaccaagtt tttatctgca
     1321 tgttcacatg catggcagaa aagggaatca gatccgatta cagatggtgt gagccaccat
     1381 gtatttgctg tgattaagaa gagaccagca tcactgaggt gaaatcttct ggaaattgtt
     1441 ttctgggagc acaaagaagc cgtgttccag agatacccaa ggttgtacct catgctgccg
     1501 ctggacttgg taatgtgtaa gagtcatttt tttttccttt taaaattttt attgattctc
     1561 tgtgagtttc acatcatgca ccccagtccc actcatctcc ctgttccctc atatccgctc
     1621 tctacccttg caatctcccc ccaaataaaa aacaaacaac aaataaaaag caaagcatag
     1681 aaaacatttc atcg
//
