LOCUS       NM_026157               4119 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus mitochondrial poly(A) polymerase (Mtpap), transcript
            variant 1, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_026157
VERSION     NM_026157.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Cheong,A., Archambault,D., Degani,R., Iverson,E., Tremblay,K.D. and
            Mager,J.
  TITLE     Nuclear-encoded mitochondrial ribosomal proteins are required to
            initiate gastrulation
  JOURNAL   Development 147 (10) (2020)
   PUBMED   32376682
  REMARK    Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC138768.3 and AEKQ02123947.1.
            
            On Jul 30, 2020 this sequence version replaced NM_026157.3.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK011546.1, AK135537.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-187               AC138768.3         113872-114058
            188-360             AC138768.3         117849-118021
            361-594             AC138768.3         118934-119167
            595-819             AC138768.3         121469-121693
            820-1031            AC138768.3         124451-124662
            1032-1258           AC138768.3         125233-125459
            1259-1351           AC138768.3         133254-133346
            1352-1425           AC138768.3         133436-133509
            1426-3714           AC138768.3         134385-136673
            3715-3915           AEKQ02123947.1     15942-16142         c
            3916-4119           AC138768.3         136875-137078
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="18"
                     /map="18 2.77 cM"
     gene            1..4119
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /note="mitochondrial poly(A) polymerase"
                     /db_xref="GeneID:67440"
                     /db_xref="MGI:MGI:1914690"
     exon            1..187
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /inference="alignment:Splign:2.1.0"
     CDS             31..1788
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /EC_number="2.7.7.19"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; PAP associated domain containing 1; poly(A) RNA
                     polymerase, mitochondrial; polynucleotide
                     adenylyltransferase; PAP-associated domain-containing
                     protein 1"
                     /codon_start=1
                     /product="poly(A) RNA polymerase, mitochondrial isoform 1
                     precursor"
                     /protein_id="NP_080433.1"
                     /db_xref="CCDS:CCDS37718.1"
                     /db_xref="GeneID:67440"
                     /db_xref="MGI:MGI:1914690"
                     /translation="MAARGVGLLTRLPVCSQRRNRIPRSISRLLSCPGTIAASIGSEE
                     QSSVVAETGIEDKTLQKKFSEVQKERREQAQRTVLIHCPNNINEKKFLKYLSQHGPVN
                     NHFFYESFGLFAVVEFCQKDSIKSLQNGTHTPTQSTEAAIPFKSRFLNLRLKNPSSQV
                     SGQPFVQTTNQSPPSSKKLFELLSYAESIEEQLNTLLKAFQLTEENIRLRHLTCSLIE
                     DIAAAYFPSCVIRPFGSSVNTFGKLGCDLDMFLDLDETGKLDVHKNTGNFFMEFQVKN
                     VPSERIATQKILSVIGECLDNFGPGCVGVQKILNARCPLVRFSHQGSGFQCDLTANNS
                     IALKSSELLYIYGSLDSRVRALVFSVRCWARAHSLTSSIPGAWITNFSLTVMVIFFLQ
                     RRSPPILPTLDSLKSIADAEDRCILEGNNCTFVQDVNKIQPSGNTETLELLIKEFFEY
                     FGNFAFNKNSINIRQGREQNKPDSSPLYIQNPFETSLNISKNVSQSQLQKFVELARDS
                     AWILEQEDKNQPFSSSRQPWGLAALLLPPGSGHTSLSRKKKKKPMSEKVKGLLASIKS
                     NSPDSSTDTSGKRTISTQA"
     exon            188..360
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /inference="alignment:Splign:2.1.0"
     exon            361..594
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /inference="alignment:Splign:2.1.0"
     exon            595..819
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /inference="alignment:Splign:2.1.0"
     exon            820..1031
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /inference="alignment:Splign:2.1.0"
     exon            1032..1258
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /inference="alignment:Splign:2.1.0"
     exon            1259..1351
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1425
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..4119
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2082..2087
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /note="hexamer: AATAAA"
     polyA_site      2106
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /note="major polyA site"
     regulatory      2629..2634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /note="hexamer: AATAAA"
     polyA_site      2651
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /note="major polyA site"
     regulatory      4097..4102
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
                     /note="hexamer: AATAAA"
     polyA_site      4115
                     /gene="Mtpap"
                     /gene_synonym="0610027A18Rik; Papd1; Tent6"
ORIGIN      
        1 ctttggcgtt tacttcctgc tgccctgata atggctgctc gcggcgtggg gttattgacc
       61 cgtttgcccg tgtgttccca gagacggaac cgaatcccgc gctctatttc caggctcctc
      121 agttgtccag ggactattgc ggcgagcatc gggagcgaag aacagtcttc ggtggttgcg
      181 gaaacaggca ttgaagacaa gaccctccaa aagaaattct ctgaggtgca gaaagagaga
      241 agagaacagg cccagcggac tgttttgata cattgtccaa ataatatcaa tgagaaaaaa
      301 tttctcaagt atttatccca gcatggacca gtcaataatc atttcttcta cgagagcttt
      361 ggtctctttg ctgttgtaga attttgccaa aaagacagta taaagtcatt gcagaatgga
      421 acacatactc caacccagag cacagaggct gcaattccat ttaagtcacg gttcttgaac
      481 ttaaggctga agaatccgtc tagccaagta tctggacagc catttgtaca gacaaccaac
      541 cagtcacctc cttcaagcaa aaagctcttt gaattactga gttatgcaga aagcatagag
      601 gagcagctga acactctcct gaaggccttc cagctcacag aagagaatat caggctccgc
      661 catctcacct gctctctgat tgaggacatt gctgctgcat attttcccag ctgtgtcatc
      721 cggccctttg gctcctcagt gaacacattt ggcaaattag gatgtgattt ggacatgttt
      781 ttagatctag atgaaactgg aaaacttgat gttcacaaga atacagggaa tttttttatg
      841 gaatttcaag tgaaaaacgt tccttcagaa agaattgcaa cccagaagat cctatctgta
      901 ataggagagt gccttgacaa ttttggccct ggctgtgtgg gtgttcagaa gatactcaat
      961 gctcgatgtc cactggtgag attctcccat cagggctctg gattccagtg tgatctgact
     1021 gctaacaata gtattgcttt gaaaagttcc gaactccttt atatatatgg ctccctggat
     1081 tcgagagtaa gagctttggt gttcagtgtg cgatgctggg ctcgggcaca ttcattaaca
     1141 agcagtattc ctggtgcttg gattacaaac ttctccttaa cagtgatggt catctttttc
     1201 ctccagagaa gatcacctcc cattcttcca actctagatt ccctaaagtc catagcagat
     1261 gctgaagata gatgtattct agaggggaac aattgtacat tcgtccagga tgtgaataaa
     1321 attcaacctt ctggaaacac agaaacatta gaattactaa taaaggaatt ttttgagtat
     1381 tttggcaatt tcgctttcaa taaaaattcc ataaacattc gacagggaag ggaacaaaac
     1441 aagcctgatt cctctcctct atacatccag aatccttttg aaacatctct caacataagc
     1501 aaaaatgtca gtcagagcca gctacaaaaa tttgtggaac tggcaagaga cagtgcctgg
     1561 attttggaac aggaagataa gaatcagcct ttttcttcga gtaggcaacc ctggggactg
     1621 gctgccctcc tgctgccacc ggggtcaggc cacacatctc tctcccggaa gaagaagaaa
     1681 aagccgatga gtgaaaaagt caaaggcttg ttagcatcca taaaaagtaa cagccctgac
     1741 agttccacag ataccagtgg aaagagaaca attagtacac aggcatgatg cagccttttg
     1801 aacttctggg aagaactgcc actaaatgtt cataggcctt aacagttata tatctgatgt
     1861 catcatgctt ttactcgatc tgaagttctg gatatttcct tccgatcaga tggagtttat
     1921 taaaatatca ctggcaaaaa attggcaaaa ggcatatttg aactctgcaa agtgaatcgt
     1981 aaaatgaatt ttacagcaac ctggttgata attctgtcac tctctagttg aaaatcattt
     2041 caaaaccctt tcagttatct tcatctatag gtgacgaatg caataaagct ccaaaatttg
     2101 atagtactct ctagtatcta ttttctattt ttcaatcaaa gttctcttat taggtgtttt
     2161 gtcttctaat tagtatataa agcattgggc ttgtatccca tcaggctttc tatgctttgt
     2221 ctttgttgat tttggttttc ctgccccttg cataccctct ctcacttttc ttcccccact
     2281 agccctcagt tgggttctgc ttcatgtagg agtgactgtt ctgctactcc tcctcttccc
     2341 ctccctgtaa gatcttcccc aaaaggtccc cagagtttca tacatgcttc acacataatc
     2401 ccaactttca cacaggagag gaaatgtaca gctctagcct cccgggtcag agctacttca
     2461 agcgaagttc cccgttgcca gttagacagg ttgggtttga tttcccaaac tgggtctctg
     2521 atacataaaa gcgcaaacac tttagtcagt gtattctgtg ttccaatcag atatgtagaa
     2581 ttgtggatat attgtacttt caagtcatat tttatttttg ttttcaagaa taaaaccctg
     2641 tttctactga attgtgataa cagtatttct aatatttctt cacttctgct tttgagatct
     2701 ctctgtgtaa gaaatggtat atacacagag aaatatgttt tacattttat tctgtagctt
     2761 tccttctgag atagagatgg tgcagtttag aaatgtataa tggttatgag gtacaaggaa
     2821 ggtgttggcc aaaacctgtc acacagtcag tgtttaatga caaaccttgg agcagggaca
     2881 agcactatat tcactcctat tctctatgat ctgtgcatat gcagcatgca gttatctacg
     2941 aatatggtcc gtgctgtgcc attttacact cctgaggttc atgttagtcc ctggagtctt
     3001 agcacttaga ggatgctcag cttgttctca gcaatgctcc tgctcttgct tttgactggg
     3061 gacattggag gcagaacacc aggtcttgtg tgtcctgggc aagcacttgt cacaagataa
     3121 atcttagccc ctactactca ttagagttca tagtctttaa tatttttcct gccattcaaa
     3181 ttttaccctt agagcttgta gtggtaaact taaagttttt ggacagaatt ctaatgagtt
     3241 ggatgatttc gggaaagtat gtttctgaag ggagtctgcc cacagagctg tgatctcaca
     3301 tctgaaggca ggccagtggc agttttcttc tacaggtatt cagcaaaggc tgctttcatt
     3361 tgttcttgaa gctcatttag ttgataggct gttttgaact cctagcctag gtgtggcagt
     3421 agaggaaggc tgtgtttgca tgtctgaata agaagagcag agacatctgt agcaaagcca
     3481 caggaaggta cagtagcttc actgtgagtt ctctgcttcc cagagcttgg catcggatgg
     3541 gtgagtagcc gttaatgttc agtctctgct aactggtaag agattactaa aaatgttata
     3601 gatagcattt ctgtctgaca tgattggatc agtagggtcg atagacaggg aggggggatt
     3661 atcccaggga aggaaaggag tcaggagtga gtgttagaga ccaggacatc tacattgctg
     3721 cttaagagtt gagcaaggct gttgtgctgc tttgattgtt ttttgcttgt ttgtttcttt
     3781 ttttgttttg ttttgtccaa tttgattaga acctagacat atctgggaag agggaatttt
     3841 cattgagaaa atgcttctat aagattggcc tgtagacagg tctgtgtgac aatttcttga
     3901 ttaactgtta atgtggaatg gcccagtctt ttggtcagtg ccaccgtggg ccagttgtcc
     3961 tggatgtata agaaaacgaa ctgaacaaga cacaagcatg ccagtaagca gcagtctctt
     4021 gacatctgct tcagtcctgc cttcaggttc ctgctcaagt tcttccctga cttccctcac
     4081 ctgttgtata cgacgaaata aaccttgcct ccccatgtt
//
