LOCUS       NM_026658               2364 bp    mRNA    linear   ROD 29-JUL-2024
DEFINITION  Mus musculus mitochondrial tRNA translation optimization 1 (Mto1),
            mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_026658
VERSION     NM_026658.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Fakruddin,M., Wei,F.Y., Suzuki,T., Asano,K., Kaieda,T., Omori,A.,
            Izumi,R., Fujimura,A., Kaitsuka,T., Miyata,K., Araki,K., Oike,Y.,
            Scorrano,L., Suzuki,T. and Tomizawa,K.
  TITLE     Defective Mitochondrial tRNA Taurine Modification Activates Global
            Proteostress and Leads to Mitochondrial Disease
  JOURNAL   Cell Rep 22 (2), 482-496 (2018)
   PUBMED   29320742
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Tischner,C., Hofer,A., Wulff,V., Stepek,J., Dumitru,I., Becker,L.,
            Haack,T., Kremer,L., Datta,A.N., Sperl,W., Floss,T., Wurst,W.,
            Chrzanowska-Lightowlers,Z., De Angelis,M.H., Klopstock,T.,
            Prokisch,H. and Wenz,T.
  TITLE     MTO1 mediates tissue specificity of OXPHOS defects via tRNA
            modification and translation optimization, which can be bypassed by
            dietary intervention
  JOURNAL   Hum Mol Genet 24 (8), 2247-2266 (2015)
   PUBMED   25552653
  REMARK    GeneRIF: MTO1 mediates tRNA modification and controls mitochondrial
            translation rate in a highly tissue-specific manner associated with
            tissue-specific oxidative phosphorylation defects.
REFERENCE   3  (bases 1 to 2364)
  AUTHORS   Becker,L., Kling,E., Schiller,E., Zeh,R., Schrewe,A., Holter,S.M.,
            Mossbrugger,I., Calzada-Wack,J., Strecker,V., Wittig,I.,
            Dumitru,I., Wenz,T., Bender,A., Aichler,M., Janik,D., Neff,F.,
            Walch,A., Quintanilla-Fend,L., Floss,T., Bekeredjian,R.,
            Gailus-Durner,V., Fuchs,H., Wurst,W., Meitinger,T., Prokisch,H., de
            Angelis,M.H. and Klopstock,T.
  TITLE     MTO1-deficient mouse model mirrors the human phenotype showing
            complex I defect and cardiomyopathy
  JOURNAL   PLoS One 9 (12), e114918 (2014)
   PUBMED   25506927
  REMARK    GeneRIF: here described is an MTO1-deficient mouse model generated
            by gene trap mutagenesis that mirrors the human phenotype showing
            complex I defect and cardiomyopathy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2364)
  AUTHORS   Li,R., Li,X., Yan,Q., Qin Mo,J. and Guan,M.X.
  TITLE     Identification and characterization of mouse MTO1 gene related to
            mitochondrial tRNA modification
  JOURNAL   Biochim Biophys Acta 1629 (1-3), 53-59 (2003)
   PUBMED   14522080
  REMARK    GeneRIF: These observations suggest that the mouse MTO1 is a
            structural and functional homolog of yeast MTO1, thereby playing a
            role in the mitochondrial tRNA modification and protein synthesis.
REFERENCE   5  (bases 1 to 2364)
  AUTHORS   Li,X., Li,R., Lin,X. and Guan,M.X.
  TITLE     Isolation and characterization of the putative nuclear modifier
            gene MTO1 involved in the pathogenesis of deafness-associated
            mitochondrial 12 S rRNA A1555G mutation
  JOURNAL   J Biol Chem 277 (30), 27256-27264 (2002)
   PUBMED   12011058
REFERENCE   6  (bases 1 to 2364)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY735135.1, CF173757.1, AF369902.1 and BB811598.1.
            
            On Mar 29, 2008 this sequence version replaced NM_026658.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK019088.1, SRR1660817.65081.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-537               BY735135.1         1-537
            538-1110            CF173757.1         73-645
            1111-2186           AF369902.1         1101-2176
            2187-2364           BB811598.1         229-406
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 43.65 cM"
     gene            1..2364
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /note="mitochondrial tRNA translation optimization 1"
                     /db_xref="GeneID:68291"
                     /db_xref="MGI:MGI:1915541"
     exon            1..301
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            302..501
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            502..619
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            620..909
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1022
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1023..1213
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1214..1344
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1345..1549
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1550..1721
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1840
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1841..2001
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2364
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2342..2347
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2361
                     /gene="Mto1"
                     /gene_synonym="2310039H01Rik; 5730419A02Rik"
                     /note="major polyA site"
ORIGIN      
        1 tacgcgctga ctcggccaag atggcgacgc ccagctggct tctactccgg tgacagttcc
       61 gggcgtctct agctagcttc cctcaacatg ttccttctcc gaggccgtgg ccattgggcc
      121 gcagcgtccc tcggcagacg gctgcctttg cgtcgcctcc gcagcgacag cgcggcgccc
      181 tgcaccccgc acttcgacgt ggtagttatc ggaggagggc acgcggggac cgaggcggcc
      241 gccgccgccg ctcgctgtgg ctcgcggacc ctgcttctca cgcaccgcgt ggacacgatc
      301 ggtcagatgt cctgtaatcc ttcctttggt ggcatcggaa aggggcattt gatgagagaa
      361 gtggatgcct tggatggtct gtgttcccgg atctgtgacc agtctggtat acattacaaa
      421 gtgctaaacc ggcgtaaggg ccctgctgtg tggggactga gagcacagat tgaccggaaa
      481 ctttataaac agaacatgca gaaagaaatc ctgagcacgc ccctactcac tgttcagaag
      541 ggagctgtag aagatctagt tcttgcagaa ccagagcctg ggtaccctgg gaagtccagg
      601 gtcagaggtg ttgtcctggc agatggaagt acaatttatg cagagagtgt aattctgact
      661 actgggacat ttctgagagg catgatcata attggattgg agatgcatcc tgcaggacgt
      721 ttgggggatc agccctccat agggttggct cagactctgg agaagttggg atttatggta
      781 ggaagactaa agactggaac tccaccccga ctcgctaaag agtccattaa tttctccatt
      841 ctaaacaagc acacaccaga tgatccatcc ataccattca gctttctcag tgacagcgtg
      901 tggatcaagc cggaagacca actgccatgt tacttgactc acaccaaccc ccgagtggat
      961 gcgattgtcc ttgagaacct tcacctgaat agccacgttc aggaaactac aaaagggccc
     1021 cgatactgtc cctctattga atcaaaggtt ttacgttttc caaatcgtct gcatcaggtt
     1081 tggttggagc ctgaaggaat ggattccgac ctcatctacc cgcaagggtt atctgttaca
     1141 ctgccagctg agttacagga gaaaatgata acatgcatca gaggcttgga gaaagccaaa
     1201 atggttcacc caggctatgg tgtgcagtat gattatttag acccccgtca gatcagtcct
     1261 tccctcgaga cacacttggt tcagcggctg ttctttgctg gacagataaa tggaaccact
     1321 ggctatgagg aagctgcagc tcagggggtg atagctggaa tcaatgccag tctacgagtc
     1381 agtcgaaagc caccctttgt tgtgagtcgc acagaaggct acataggagt cttgattgat
     1441 gacctcacta ccctgggcac cagtgagcca taccgaatgt tcactagcag agtagagttc
     1501 cgattgtcac tgcgccccga taatgctgac acccggctca cgttccgagc ccataaggaa
     1561 gctggctgtg tgtcctcaca gcgatttgaa agagctttgt ggatgaagtc atctttagaa
     1621 gaaggtattt ctgtgttgaa atccattaaa ttttcaagct ccaaatggaa aaagttaatt
     1681 ccacaaattc ctataagtat taatagaagc cttcctgtca gtgctctgga tgttcttaag
     1741 tatgaagaag tggacatgga gtcgctcgtc ggcgtgcttc ccgagccctt ggagaagtat
     1801 actgcatgta gagagctcgc cagaagactg aaaatagaag ccagttatga gtcagtattg
     1861 tcatatcagc tacaagaaat aaaggaggtt cagcaagatg aagctttgca gcttccacac
     1921 gagctcgact acctaaccat cagggatgtg tcattgtccc aggaagtccg ggagaagcta
     1981 cacttgagtc gcccacagac gattggggct gctagtcgta ttcctggagt gacaccagct
     2041 gccatcatca atctgctcag atttgtgagg agcacgcggc agagcagaca gcagtgacag
     2101 aacgccaggc caatcggtgc ttgtgaactt tcatttcata gaggacttag agtgagggct
     2161 tagggcaatg gttttcagcc ccgtaaaaca gttccttgtg ttgtggtgag ccccaaacac
     2221 acaattattt cattcctact tcatactgtt acgaatcata atgtaaatat ctaatattca
     2281 atccctgtgg gagtctcaag ccatgggctg agaaccgctg gcttagagag tatgaatagg
     2341 aaataaagat gacactgaaa atac
//
