LOCUS       NM_001256102            4968 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus mitochondrial translational initiation factor 3
            (Mtif3), transcript variant 4, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_001256102
VERSION     NM_001256102.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Richman,T.R., Ermer,J.A., Baker,J., Siira,S.J., Kile,B.T.,
            Linden,M.D., Rackham,O. and Filipovska,A.
  TITLE     Mitochondrial gene expression is required for platelet function and
            blood clotting
  JOURNAL   Cell Rep 42 (11), 113312 (2023)
   PUBMED   37889747
REFERENCE   3  (bases 1 to 4968)
  AUTHORS   Rudler,D.L., Hughes,L.A., Perks,K.L., Richman,T.R., Kuznetsova,I.,
            Ermer,J.A., Abudulai,L.N., Shearwood,A.J., Viola,H.M., Hool,L.C.,
            Siira,S.J., Rackham,O. and Filipovska,A.
  TITLE     Fidelity of translation initiation is required for coordinated
            respiratory complex assembly
  JOURNAL   Sci Adv 5 (12), eaay2118 (2019)
   PUBMED   31903419
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4968)
  AUTHORS   Koc,E.C. and Spremulli,L.L.
  TITLE     Identification of mammalian mitochondrial translational initiation
            factor 3 and examination of its role in initiation complex
            formation with natural mRNAs
  JOURNAL   J Biol Chem 277 (38), 35541-35549 (2002)
   PUBMED   12095986
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY284518.1, BC115684.1, BC115685.1, BC052439.1 and BM944410.1.
            
            Transcript Variant: This variant (4) differs in the 5' UTR compared
            to variant 1. All five variants encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660813.438803.1, BC115684.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-48                BY284518.1         1-48
            49-743              BC115684.1         1-695
            744-942             BC115685.1         867-1065
            943-2425            BC052439.1         908-2390
            2426-2666           BM944410.1         479-719
            2667-4968           BC052439.1         2631-4932
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 86.57 cM"
     gene            1..4968
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
                     /note="mitochondrial translational initiation factor 3"
                     /db_xref="GeneID:76366"
                     /db_xref="MGI:MGI:1923616"
     exon            1..75
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            76..527
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..907
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
                     /note="IF3mt; IF-3Mt; IF3(mt); IF-3(Mt)"
                     /codon_start=1
                     /product="translation initiation factor IF-3,
                     mitochondrial"
                     /protein_id="NP_001243031.1"
                     /db_xref="CCDS:CCDS19874.1"
                     /db_xref="GeneID:76366"
                     /db_xref="MGI:MGI:1923616"
                     /translation="MAVLLMRLMLQTTKLDHNLIGRCLQRHAVKPDPAQLSLSASTPK
                     LLYLTSAKGFSTAGDPQGERKQKRRDAFSNTGRKISERIIRVLDEKGMDLGMMHRADV
                     IRLMNKQDLRLVQRNTSSEPPEYQLMTGEQIHQERLKLREQEKAKPKTGPTMTKELVF
                     SSNIGQHDLDTKSKQIQQWIEKKYHVQVTIKRRKDAEQSEEETEEIFNQILQTMPDIA
                     TFSSRPKAIRGGTASMCVFRHLSKKEEKAYRESQESQRRDTLSKDDDGNSKESDVVCQ
                     "
     exon            528..685
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            686..4928
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1551..1556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1572
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
                     /note="major polyA site"
     polyA_site      1981
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
     regulatory      4907..4912
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
                     /note="hexamer: AATAAA"
     polyA_site      4928
                     /gene="Mtif3"
                     /gene_synonym="2810012L14Rik"
ORIGIN      
        1 ggaagccgac gcggtgctcc ggcggagtcc aggtacggac ctgctagttc ttcctgctcg
       61 cctaccccgg cccaggatgg ctgttcttct gatgaggttg atgctacaaa ctacaaagct
      121 tgaccataac ttaattggaa gatgtttgca aagacacgct gtgaagccag atccagcaca
      181 gctgtccctg tctgcgtcca ccccaaagct gttgtacctg acttctgcaa aaggttttag
      241 tactgctgga gacccacagg gtgagaggaa acagaaaaga agggatgctt tctctaacac
      301 cggccggaag attagtgagc ggattatccg cgtcctggat gagaagggca tggacctggg
      361 gatgatgcat cgagcagatg tgatcagact catgaacaag caggacctga ggctggtgca
      421 gaggaacacc agctcagagc caccggagta ccagcttatg accggagagc agatccacca
      481 ggagcggcta aagctccggg aacaggaaaa ggccaaaccc aaaactggac caactatgac
      541 gaaagagctc gttttctctt caaatattgg acagcacgat ttagacacca agagtaaaca
      601 gatccagcag tggatagaga agaaatacca cgttcaagtc acgataaaga ggaggaaaga
      661 tgcggagcag tcagaggagg aaacggagga gatatttaac cagattctcc agactatgcc
      721 tgacatagca accttctcaa gcagaccaaa agccatcaga ggagggactg cttccatgtg
      781 tgtttttcgt cacttgagca agaaagaaga aaaggcgtac agagaatccc aggagagcca
      841 gagaagagac actttgagca aagacgacga cggaaacagc aaagagtcgg atgttgtgtg
      901 tcagtgactt taataggaaa agtgcaggcc aggagcctct gctggccgcc ctgaagtctt
      961 cccgcctagc agacagtgag taaggcggct ggcgggggca gctgggcctc tgggctgctg
     1021 ctggactctg cgctggcgtt aggcaaggag tagtcgggct cttgtttcct cttaggaggg
     1081 aagtacccac tgaggcgaga ggccaagctg cgtctctccc gaggggctct taccttcagg
     1141 agtgaggaag acagacaact gagttgccca ttacacaacg gggatccact ccaacagatg
     1201 agcccctgat gggagtcaca ccctcagcag gcacagacac gcaggatggg gtatgcggat
     1261 ggacagacgc caagcaggcc tcacagccga gtgctggccc cagcagctgc cacttacttt
     1321 gagcttcatc ctcttcttgt cgggatcgtg cacgacactg tgcctcatga gactctgctg
     1381 cagtgcaagg gaaagaagga cgcttgacct gggcctgcag acgtggctca gtgcttacag
     1441 cattcgctgc tcgaggagga cacgggtttg gttcccagca cccgtactcc agttccagta
     1501 aaaactcttg gtgtctgcta accttaagat gcagtggtcc agtgtgcaat aataaactac
     1561 cagctgcctt tctgttgtgt agtgaatctg accttcacct gactccctaa ccagagccaa
     1621 ctccttggca gagcgagcga ctgtcagcct gctgacacgc atgggattta cttcttcccg
     1681 gtcctcctga gaagggaaag ccggctatgc ttgaacatcc aggctcatgc tggggtaact
     1741 gaatctacct cagctgcgtc cctaagtgcc agggggaaat ctgacctctg ccctgctggc
     1801 tatgctgaca gtgctgacca cgggaccttt gctgtgggct ggtctgggtt atgttcagtg
     1861 actttgtaat ccacagtaag agctctttag aaaccagcta ggcttaggca aactgttcag
     1921 ttccctacta ggaaccatgt tcccacgcga tccatgaatc atgccaagat tccagagtct
     1981 agaggctgtg catttgtgct acccacctcc tctcccagac cctaggcgac ggtcagaagc
     2041 tcctaatcac ctcagaacat cctctaagta ggaatacagg ccccgggctc tcccactcag
     2101 catggctaga atcccccagg agtggttctc aacctgctac tcatgactcc tctggggtca
     2161 tataccagat cgcctgaata tcattattta caatccatac cagtagcaaa attatagtta
     2221 tgaagtagca actaaataat attatggttg ggggtcccca caacatgagg aactgttatt
     2281 gggtcccagt attaggaagg ttgagaacca ctgctctaga ggtcctaaat aggcctgttt
     2341 agcctcccaa ccccagggct cctgcatttc caagttctgc aggctggaca tgaacacacc
     2401 agcctggagc gggagacact cactttcatt gcgaatgtct tgccacagcc agcgtgctca
     2461 cacacaaatg ggcgcttttc ctcgtggaag gagagaatgt ggctctgcag gttgaacacg
     2521 gttgtgtagg ttcttccgca gccttgccgc ggacagcggt acacatccct ttccggggcg
     2581 tgagtcttca tgtgctgctt aaggtaatct ctgcgcttga acatcctctg acatacattg
     2641 caggttatgt cctctgggag gagaaacaca cacgtgtaac cagcgtccca tgagtaaccc
     2701 gtctggtcct atgactacac acaatctggg gacttcacat gggaaatgtc agcttgtctc
     2761 actgctatgc tggtttcaaa ttcctcaagt tcactcatgg ctcccaacat cctgagtcct
     2821 cgcctgtcaa ggagcaggcg tcctcggtgt gataactgag cacgaggaag tatgccctca
     2881 tagcatctac ctttaccctc tgacacagta gcatctgcta agtcctgcag ggcctcacga
     2941 acacccgggc acagccgcac cagctctgag gcctgcaggg ctccctcgac tgtcacagcc
     3001 caggaagcca gcactaccct gaactcgatg cagagccccc ttcctttaca gcgcaagtgg
     3061 gtatctcact ctctcagagt acaccatgca ctccccagag ctcagagcac acacaccatg
     3121 ggctccccgg ggttcagagc acaccgggct ctccccaggg ccgatgcgct tccacagctc
     3181 ctgcctacct tacctttatg ggcttctctc atgtgtttca ggagctctgt ccacgttttt
     3241 cccatgaaag aacatccctt ttgacacagg tagcctgttc aaaacaaaaa caaaacaagt
     3301 tacccacagc ctgttgaatg cccagggcaa tccctaatga cagccaagaa agcccgtgcc
     3361 aagtgcactc ccctctctga agtgtgctgc tctgctgggt gcaatgcagc gggctccagt
     3421 ggcccctgct ctgaaagctc ccatcagcca gacttttgca cacttgagtg gcacataacc
     3481 aggtagcgta ggaacagtat agcaccagtg gacgtggcaa agaggatagc ggcaaaacac
     3541 acagcacagt gtctcttggc ataagaggca aacactcaaa taacacaaca ggcgcttgct
     3601 tgtcacagaa ctttgtctac tcagcgtgac tacagaatag acccagccta ccctgccggt
     3661 ggcatgttcc tcgcggccgg cacagtcccc atctacgtac cctcgtgaac tttcccatgc
     3721 cgtttcagcc tgctgggcga ggcaaagtgc ttcccgcatc cctcgtgggt acacctagga
     3781 caatagcagg caaagcatca gccaggccct ttccatggac aattctaaga ttccctcttc
     3841 tcccacacca aagcccatgt cttcccacac cacacctgtc atccaaaaca aagagtaagt
     3901 acagtcccga atatggacat agtggcttgg ccagcttggg agcaaaacaa gccatcctga
     3961 gaaaaagtca catcttttgt ttgtgttatc aaactaaaag ttctctgaat tctggaaatg
     4021 tggctacaga agcgacatca agaggctgtc tgactcggtt tgcactggac cagacacaga
     4081 gctgttgtct ggggtctgcc caatatgctg catacggtca gatgagaaca cagcacagag
     4141 gctggggacc agcaccagaa acacacgaga atgtacagca gtcggtgcag agctccaggc
     4201 actggcagca caaacaggcc acagggctgc tggtaagacc tcggtcactg tagctgcacc
     4261 agtgcaaaga gcagcctatg ggagccaggg gcaaggcccc atcacccgga ctgtgcaggg
     4321 agacactgcc ctaggaggga ggcaggcagg taacctggca cccctgccca cctcctcttt
     4381 gtccctcttg agtactcagg aatccctcaa aacaagcagg gcctcattac tcctgttcta
     4441 tagtaagaaa aaaaaaagcc ctcatgacac agagccttcc tcgggatcct gatcttagcg
     4501 ttctactctg aatcccagag cgctggcaaa gcaccaacct gaagagcggc tcgctggtgt
     4561 gctggcactg atgggttctc agctgctggt gcttcttaaa ggccttcttg caaccctcat
     4621 aactgcactg tttaaaaaaa ataaatgcac ggtttagtac aggcagggga tcatcagttg
     4681 ctgaagtcct ttaaaaggca ggagagattg ctcaatggtt agggacactt gcttctctgg
     4741 tcccaggttt ggactgcagc acccacgtgg tgcctcacaa ccatctgact ccagttccac
     4801 aggacctcac accctcttct gacctccaca ggcaccgagc acgcatatgg tatatagata
     4861 catatgcagg caaaacgctt gcacataaaa ccttttttaa ctaaacaata aagtctctct
     4921 ggatctgaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa
//
