LOCUS       NM_133767               3017 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus mitochondrial translational initiation factor 2
            (Mtif2), transcript variant 1, mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_133767
VERSION     NM_133767.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Liu,X., Jiao,Y., Cao,Y., Deng,N., Ma,Y., Hasty,K.A., Kang,A.,
            Chen,H., Stuart,J.M. and Gu,W.
  TITLE     Decreased expression levels of Ifi genes is associated to the
            increased resistance to spontaneous arthritis disease in mice
            deficiency of IL-1RA
  JOURNAL   BMC Immunol 17 (1), 25 (2016)
   PUBMED   27480124
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Girard,A., Sachidanandam,R., Hannon,G.J. and Carmell,M.A.
  TITLE     A germline-specific class of small RNAs binds mammalian Piwi
            proteins
  JOURNAL   Nature 442 (7099), 199-202 (2006)
   PUBMED   16751776
REFERENCE   3  (bases 1 to 3017)
  AUTHORS   Jin,W.H., Dai,J., Zhou,H., Xia,Q.C., Zou,H.F. and Zeng,R.
  TITLE     Phosphoproteome analysis of mouse liver using immobilized metal
            affinity purification and linear ion trap mass spectrometry
  JOURNAL   Rapid Commun Mass Spectrom 18 (18), 2169-2176 (2004)
   PUBMED   15378723
REFERENCE   4  (bases 1 to 3017)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   5  (bases 1 to 3017)
  AUTHORS   Koc,E.C. and Spremulli,L.L.
  TITLE     Identification of mammalian mitochondrial translational initiation
            factor 3 and examination of its role in initiation complex
            formation with natural mRNAs
  JOURNAL   J Biol Chem 277 (38), 35541-35549 (2002)
   PUBMED   12095986
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX284634.8, CK620846.1, AK146961.1 and CF737093.1.
            
            On Aug 18, 2013 this sequence version replaced NM_133767.2.
            
            Transcript Variant: This variant (1) encodes isoform (1). Both
            variants 1 and 2 encode the same isoform.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146961.1, SRR7652917.988793.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                BX284634.8         101567-101577
            12-60               CK620846.1         1-49
            61-2808             AK146961.1         1-2748
            2809-3017           CF737093.1         274-482
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 16.77 cM"
     gene            1..3017
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /note="mitochondrial translational initiation factor 2"
                     /db_xref="GeneID:76784"
                     /db_xref="MGI:MGI:1924034"
     exon            1..447
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            448..673
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     CDS             455..2638
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; translation initiation factor IF-2,
                     mitochondrial"
                     /codon_start=1
                     /product="translation initiation factor IF-2,
                     mitochondrial isoform 1 precursor"
                     /protein_id="NP_598528.2"
                     /db_xref="CCDS:CCDS24496.1"
                     /db_xref="GeneID:76784"
                     /db_xref="MGI:MGI:1924034"
                     /translation="MNQKLLKLENLLRFHTICRQVHSPSQRRLLAWCRHGFAPASSVW
                     RDLLGARSWQTDMLIGSALHQHRLLVTKKEKRPPRSQLSPVKTKKEVEVWVGMTVEDL
                     ASAMAKDIDCVYEALLNTAIDVDSLEANSHLDEVWIKEVIKKAGMKLKWSKLKQERIR
                     ENKDAVRRPGTDPALLKPRSPVVTVMGHVDHGKTTLLDKLRETQVAAMEVGGITQHIG
                     AFLVSLPSGEKITFLDTPGHAAFSAMRARGAQVTDIVVLVVAADDGVMKQTVESIQHA
                     KDAEVPIILAINKCDKTDADPEKVKKELLAYDVVCEEYGGDVQAVHVSALTGDNLMAL
                     AEATIALAEILELKADPTGPVEGTVIESFTDKGRGPVTTAIIQRGTLRKGSILVAGKS
                     WAKVRLIFDENGKILNEAYPSMPVGIIGWRDLPSAGDEILEVESEPRAREVIEWRKSE
                     QKEEKGKDDLKIMEEKRREHQEAHRKAREKYGSLHWKERSYIKFLERKQQRPLKPKEK
                     VERQSNVLPIIIKGDVDGSVEAILNLLDTYDASHECELELVHFGLGDISENDVTFAET
                     FDGVIYGFNVEAGSAIQQSAAQKGVKIKLHKIIYHLIEDLQEELSSRLPHTLEEYPIG
                     EASILATFTVTEGKKKIPVAGCRVQKGQLERHKKFKLIRNGQVIWKGSLTSLKHHKDD
                     ISVIKTGMDCGLSLDEEKVEFKPGDQVICYEENKVPTKTSWDPGF"
     exon            674..785
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            786..957
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1118
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1295
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            1296..1435
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            1436..1560
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1765
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..2018
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2159
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2324
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            2325..2465
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     exon            2466..3005
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2987..2992
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /note="hexamer: AATAAT"
     polyA_site      3005
                     /gene="Mtif2"
                     /gene_synonym="2310038D14Rik; 2410112O06Rik; IF-2(Mt);
                     IF-2mt; IF2(mt)"
                     /note="major polyA site"
ORIGIN      
        1 accggaagca cgacggggac ctcacggaag ggacggactg aatgacccac aattctcctg
       61 ggtccggagg gcgacctcgc ctgggagttg agtgagtggg gtctctgcgc tagggtggtc
      121 gcgagtgcca gtgtcacttg ccgcggcttc ggagtccacg agcgctcagg tgcgctcccc
      181 agaggcccgc gtggcagcgg cggtgtttga tttttcacaa cgattttttt tttcctttcc
      241 aaatcctctt actggtctat tcagacattt ccgttaatgc agctacgctt ttgcagcatt
      301 cgcacgccct ctccgctatg ggaattgtca gcctgagttc tcggttgccg ctaaccttat
      361 ggaagtagta atgacccctt cttgagtcca ctgtcctctg taaagtgaga ggcgttggga
      421 tatttaacaa ccaagatttc ttcccaggag aaatatgaac cagaagctac tgaagttgga
      481 aaacttgcta cgatttcata ctatttgtag gcaggtgcac agcccaagtc aaagaagact
      541 cttagcatgg tgtagacatg ggtttgcacc ggcttcctca gtgtggagag atctgctggg
      601 tgcccggtcc tggcagacag acatgctcat tggttcagct ttgcatcagc acagacttct
      661 tgtaacaaag aaggaaaaaa gaccaccaag atctcagttg tctccagtaa aaactaaaaa
      721 agaggtagaa gtatgggttg gaatgactgt tgaagatcta gccagtgcca tggcaaaaga
      781 tatagattgt gtatacgaag ctttattgaa cactgccatt gacgtagatt cattagaagc
      841 aaactcacat ttagatgaag tctggatcaa agaagttata aagaaggcag gaatgaagct
      901 gaaatggagc aaattaaaac aggaaagaat tagagaaaat aaagatgctg tgagaaggcc
      961 tgggacagat ccagctttat taaaaccaag gtccccagtt gttactgtaa tgggccatgt
     1021 tgatcatggg aaaacgacct tacttgacaa acttcgagaa actcaagttg cagcgatgga
     1081 agtcggaggc atcactcaac acattggtgc ttttcttgtc tctctgcctt ctggagaaaa
     1141 gataactttt cttgatactc ctggacatgc tgccttctca gcaatgagag ccagaggagc
     1201 tcaggtcacc gacattgttg tgttggttgt agctgcagat gatggggtaa tgaaacaaac
     1261 tgtggaatcc attcagcatg caaaagatgc agaagttcct attatccttg caatcaataa
     1321 gtgcgacaag acagatgctg atcctgaaaa ggtgaaaaaa gagctcctag cttacgatgt
     1381 ggtgtgtgaa gagtatggtg gtgatgttca agcagtgcac gtctctgcac ttacgggcga
     1441 taacctgatg gctttggcag aagcaacaat tgctctcgca gaaatcttgg aactgaaagc
     1501 agatcccacc ggtccagtgg aaggaacagt aatagagtct ttcacagaca aaggaagagg
     1561 tcctgttaca acagctataa ttcaaagagg aactctgaga aaaggctcaa ttctagttgc
     1621 tgggaagagt tgggcaaaag ttcgactaat atttgatgaa aatggaaaaa tacttaatga
     1681 ggcctatccc agcatgccag tgggaatcat aggctggaga gaccttcctt ctgcaggaga
     1741 tgaaattctt gaagtagaat ctgagccaag ggcccgtgaa gttattgaat ggaggaagtc
     1801 tgagcaaaaa gaagaaaaag gcaaagatga cctgaaaata atggaagaaa agcgaaggga
     1861 acaccaagaa gcgcatcgga aagcccgtga gaagtatggc agtctgcact ggaaagagag
     1921 atcatatata aagttccttg aaaggaaaca acagagaccc ttaaagccca aagaaaaggt
     1981 agaaaggcaa tcaaatgtac ttcctataat tattaaaggt gacgttgatg ggtctgtgga
     2041 ggccatcttg aaccttctgg atacctacga tgcttcccac gaatgtgaac tcgaattagt
     2101 acattttgga ttgggtgaca tcagtgaaaa tgatgttacc tttgctgaga catttgatgg
     2161 tgttatttat ggttttaatg tggaggcagg cagtgctatt cagcagtcag ctgcacaaaa
     2221 gggagttaag attaaacttc acaaaatcat ctaccatctt attgaagatt tgcaggagga
     2281 actaagcagc agattgcccc acacactgga ggagtaccca ataggtgagg cttctatact
     2341 agctaccttc actgtaacag aagggaagaa aaaaattcct gttgctggct gcagagttca
     2401 aaagggacaa ttagaaagac ataagaagtt taaattaatc cgaaacggcc aagttatttg
     2461 gaagggatca ttaacctctc tgaaacacca taaagatgac atttcagtta taaaaactgg
     2521 tatggactgt ggtcttagtt tagatgaaga aaaagtcgaa ttcaaaccgg gagatcaagt
     2581 catttgttat gaagaaaata aagttccaac taagacttcc tgggatccag gattttaaaa
     2641 gcacattaaa aatggtaaat gggaagccgg gtgtggtggt gcacgccttt aatcccagca
     2701 cttgggtggc agaggcaggc ggatttctga gttcgaggcc agcctggtct acaaagtgag
     2761 ttccaggaca gccagagcta tacagagaaa ccctgtctcg gaaaaaccaa aaaaataaaa
     2821 aaaaaaaaaa aaggtaaatg ggctggagag atggctcact gactgctctt ctggaggtcc
     2881 tgagttcaaa tcccagccac cacatggtgg ctcacaacca tccgtaatga gatctgacac
     2941 tcttctggag tgtctgaaga cagctacagt gtacttatat ataataaata attcttttaa
     3001 aaaaaaaaaa aaaaaaa
//
