LOCUS       NM_026829                768 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus 5, 10-methenyltetrahydrofolate synthetase (Mthfs),
            transcript variant 1, mRNA.
ACCESSION   NM_026829 XM_904176
VERSION     NM_026829.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Lumayag,S., Haldin,C.E., Corbett,N.J., Wahlin,K.J., Cowan,C.,
            Turturro,S., Larsen,P.E., Kovacs,B., Witmer,P.D., Valle,D.,
            Zack,D.J., Nicholson,D.A. and Xu,S.
  TITLE     Inactivation of the microRNA-183/96/182 cluster results in
            syndromic retinal degeneration
  JOURNAL   Proc Natl Acad Sci U S A 110 (6), E507-E516 (2013)
   PUBMED   23341629
REFERENCE   2  (bases 1 to 768)
  AUTHORS   Field,M.S., Anderson,D.D. and Stover,P.J.
  TITLE     Mthfs is an Essential Gene in Mice and a Component of the
            Purinosome
  JOURNAL   Front Genet 2, 36 (2011)
   PUBMED   22303332
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 768)
  AUTHORS   Field,M.S., Anguera,M.C., Page,R. and Stover,P.J.
  TITLE     5,10-Methenyltetrahydrofolate synthetase activity is increased in
            tumors and modifies the efficacy of antipurine LY309887
  JOURNAL   Arch Biochem Biophys 481 (2), 145-150 (2009)
   PUBMED   19022216
REFERENCE   4  (bases 1 to 768)
  AUTHORS   Liu,S.P., Li,Y.S., Chen,Y.J., Chiang,E.P., Li,A.F., Lee,Y.H.,
            Tsai,T.F., Hsiao,M., Huang,S.F. and Chen,Y.M.
  TITLE     Glycine N-methyltransferase-/- mice develop chronic hepatitis and
            glycogen storage disease in the liver
  JOURNAL   Hepatology 46 (5), 1413-1425 (2007)
   PUBMED   17937387
  REMARK    Erratum:[Hepatology. 2008 Feb;47(2):769. Hwang, Shiu-Feng
            [corrected to Huang, Shiu-Feng]]
REFERENCE   5  (bases 1 to 768)
  AUTHORS   Field,M.S., Szebenyi,D.M., Perry,C.A. and Stover,P.J.
  TITLE     Inhibition of 5,10-methenyltetrahydrofolate synthetase
  JOURNAL   Arch Biochem Biophys 458 (2), 194-201 (2007)
   PUBMED   17250800
REFERENCE   6  (bases 1 to 768)
  AUTHORS   Anguera,M.C. and Stover,P.J.
  TITLE     Methenyltetrahydrofolate synthetase is a high-affinity
            catecholamine-binding protein
  JOURNAL   Arch Biochem Biophys 455 (2), 175-187 (2006)
   PUBMED   17055997
  REMARK    GeneRIF: Expression of the MTHFS cDNA in MCF-7 cells increased the
            concentration of NADA required to deplete cellular folate.
REFERENCE   7  (bases 1 to 768)
  AUTHORS   Saba,L., Bhave,S.V., Grahame,N., Bice,P., Lapadat,R., Belknap,J.,
            Hoffman,P.L. and Tabakoff,B.
  TITLE     Candidate genes and their regulatory elements: alcohol preference
            and tolerance
  JOURNAL   Mamm Genome 17 (6), 669-688 (2006)
   PUBMED   16783646
REFERENCE   8  (bases 1 to 768)
  AUTHORS   Field,M.S., Szebenyi,D.M. and Stover,P.J.
  TITLE     Regulation of de novo purine biosynthesis by
            methenyltetrahydrofolate synthetase in neuroblastoma
  JOURNAL   J Biol Chem 281 (7), 4215-4221 (2006)
   PUBMED   16365037
REFERENCE   9  (bases 1 to 768)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   10 (bases 1 to 768)
  AUTHORS   VanBuren,V., Piao,Y., Dudekula,D.B., Qian,Y., Carter,M.G.,
            Martin,P.R., Stagg,C.A., Bassey,U.C., Aiba,K., Hamatani,T.,
            Kargul,G.J., Luo,A.G., Kelso,J., Hide,W. and Ko,M.S.
  TITLE     Assembly, verification, and initial annotation of the NIA mouse
            7.4K cDNA clone set
  JOURNAL   Genome Res 12 (12), 1999-2003 (2002)
   PUBMED   12466305
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK004092.1.
            
            On Apr 6, 2007 this sequence version replaced NM_026829.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK004092.1, AK158264.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-768               AK004092.1         2-769
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 47.24 cM"
     gene            1..768
                     /gene="Mthfs"
                     /gene_synonym="1110034I12Rik; 2310020H23Rik"
                     /note="5, 10-methenyltetrahydrofolate synthetase"
                     /db_xref="GeneID:107885"
                     /db_xref="MGI:MGI:1340032"
     exon            1..122
                     /gene="Mthfs"
                     /gene_synonym="1110034I12Rik; 2310020H23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             6..617
                     /gene="Mthfs"
                     /gene_synonym="1110034I12Rik; 2310020H23Rik"
                     /EC_number="6.3.3.2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     5-formyltetrahydrofolate cyclo-ligase; methenyl-THF
                     synthetase; 5,10-methenyl-tetrahydrofolate synthetase"
                     /codon_start=1
                     /product="5-formyltetrahydrofolate cyclo-ligase isoform 1"
                     /protein_id="NP_081105.1"
                     /db_xref="CCDS:CCDS23394.1"
                     /db_xref="GeneID:107885"
                     /db_xref="MGI:MGI:1340032"
                     /translation="MAAVTVNSAKRGLRAELKQRLRALSAEERLRQSLLLTQKVIAHN
                     QYQNSKRISIFLSMQDEVETEVIIKDIFKQGKICFIPRYQFQSNHMDMVRLTSSEEIA
                     LLPKTSWNIHQPGEGDVREEALSTGGLDLIFLPGLGFDKDGNRLGRGKGYYDTYLKRC
                     VQHQEVKPYTMALAFKEQICPQIPVDEHDMKVDEVLYEDSPAS"
     exon            123..384
                     /gene="Mthfs"
                     /gene_synonym="1110034I12Rik; 2310020H23Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            385..768
                     /gene="Mthfs"
                     /gene_synonym="1110034I12Rik; 2310020H23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      744..749
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mthfs"
                     /gene_synonym="1110034I12Rik; 2310020H23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      765
                     /gene="Mthfs"
                     /gene_synonym="1110034I12Rik; 2310020H23Rik"
                     /note="major polyA site"
ORIGIN      
        1 caggaatggc ggcggtcacg gtaaacagtg ccaagcgggg cctgcgggcc gagctgaagc
       61 agcgcttgag ggccttgagc gcggaggaac ggctgcgcca gtctctcctc ctcacgcaga
      121 aggtgattgc ccacaatcag tatcagaatt ccaaaagaat ttccatcttt ctaagcatgc
      181 aagatgaagt tgagacggaa gtaatcatca aggacatttt caaacaaggc aaaatctgct
      241 tcatccctcg gtaccaattc cagagcaatc acatggacat ggtgaggtta acgtcatctg
      301 aagaaattgc tttacttccc aagacatctt ggaatattca tcagcctggt gagggagatg
      361 ttcgggagga ggccttatcc actggtggac ttgacctcat cttccttcct ggcctcgggt
      421 ttgacaaaga tggcaaccgg ctggggcggg gtaaggggta ctatgacacc tacctgaagc
      481 gctgtgtaca gcatcaggaa gtgaagccct acaccatggc tttggctttt aaagagcaga
      541 tctgtcccca gatcccagtg gatgagcatg acatgaaggt agatgaagtc ctttatgaag
      601 attccccagc atcctaagtt acaccacgac ggctacactt actgctctgt cccggtgtaa
      661 agaacatatg tatatttttc tcttgtcaaa atgaatcaaa atggcacatt aaagtgaata
      721 tgggttgtga ttttaagtat aaaaataaaa taatataaaa tcctattt
//
