LOCUS       NM_008638               2070 bp    mRNA    linear   ROD 05-JUN-2024
DEFINITION  Mus musculus methylenetetrahydrofolate dehydrogenase (NAD+
            dependent), methenyltetrahydrofolate cyclohydrolase (Mthfd2), mRNA;
            nuclear gene for mitochondrial product.
ACCESSION   NM_008638
VERSION     NM_008638.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Cui,Y., Li,Z., Ni,L., Yu,S., Shan,X., Hu,P., Ji,Z., Jing,W.,
            Zhou,Y., Wang,B., Dong,H., Zhou,J., Xie,K. and Yu,Q.
  TITLE     Induction of MTHFD2 in Macrophages Inhibits Reactive Oxygen
            Species-mediated NF-kappaB Activation and Protects against
            Inflammatory Responses
  JOURNAL   J Immunol 212 (8), 1345-1356 (2024)
   PUBMED   38407485
  REMARK    GeneRIF: Induction of MTHFD2 in Macrophages Inhibits Reactive
            Oxygen Species-mediated NF-kappaB Activation and Protects against
            Inflammatory Responses.
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2070)
  AUTHORS   Wang,Y., Hongu,T., Nishimura,T., Takeuchi,Y., Takano,H.,
            Daikoku,T., Yao,R. and Gotoh,N.
  TITLE     Mitochondrial one-carbon metabolic enzyme MTHFD2 facilitates
            mammary gland development during pregnancy
  JOURNAL   Biochem Biophys Res Commun 674, 183-189 (2023)
   PUBMED   37450958
  REMARK    GeneRIF: Mitochondrial one-carbon metabolic enzyme MTHFD2
            facilitates mammary gland development during pregnancy.
REFERENCE   4  (bases 1 to 2070)
  AUTHORS   Shang,M., Ni,L., Shan,X., Cui,Y., Hu,P., Ji,Z., Shen,L., Zhang,Y.,
            Zhou,J., Bing,Chen., Wang,T. and Yu,Q.
  TITLE     MTHFD2 reprograms macrophage polarization by inhibiting PTEN
  JOURNAL   Cell Rep 42 (5), 112481 (2023)
   PUBMED   37149861
REFERENCE   5  (bases 1 to 2070)
  AUTHORS   Huynh,H., Zhu,S., Lee,S., Bao,Y., Pang,J., Nguyen,A., Gu,Y.,
            Chen,C., Ouyang,K., Evans,S.M. and Fang,X.
  TITLE     DELE1 is protective for mitochondrial cardiomyopathy
  JOURNAL   J Mol Cell Cardiol 175, 44-48 (2023)
   PUBMED   36539111
REFERENCE   6  (bases 1 to 2070)
  AUTHORS   Di Pietro,E., Sirois,J., Tremblay,M.L. and MacKenzie,R.E.
  TITLE     Mitochondrial NAD-dependent methylenetetrahydrofolate
            dehydrogenase-methenyltetrahydrofolate cyclohydrolase is essential
            for embryonic development
  JOURNAL   Mol Cell Biol 22 (12), 4158-4166 (2002)
   PUBMED   12024029
REFERENCE   7  (bases 1 to 2070)
  AUTHORS   Abidari,J.M., Gonzales,E.T., Inoue,K., Lupski,J.R., Karsenty,G. and
            Katsanis,N.
  TITLE     Identification of novel genes expressed during metanephric
            induction through single-cell library screening
  JOURNAL   Kidney Int 57 (6), 2221-2228 (2000)
   PUBMED   10844592
REFERENCE   8  (bases 1 to 2070)
  AUTHORS   Belanger,C., Peri,K.G. and MacKenzie,R.E.
  TITLE     Analysis of the promoter region of the gene encoding NAD-dependent
            methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate
            cyclohydrolase
  JOURNAL   Nucleic Acids Res 19 (16), 4341-4345 (1991)
   PUBMED   1843253
REFERENCE   9  (bases 1 to 2070)
  AUTHORS   Belanger,C. and MacKenzie,R.E.
  TITLE     Structural organization of the murine gene encoding NAD-dependent
            methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate
            cyclohydrolase
  JOURNAL   Gene 97 (2), 283-288 (1991)
   PUBMED   1999293
REFERENCE   10 (bases 1 to 2070)
  AUTHORS   Belanger,C. and MacKenzie,R.E.
  TITLE     Isolation and characterization of cDNA clones encoding the murine
            NAD-dependent methylenetetrahydrofolate
            dehydrogenase-methenyltetrahydrofolate cyclohydrolase
  JOURNAL   J Biol Chem 264 (9), 4837-4843 (1989)
   PUBMED   2647744
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY068806.1, AK159680.1 and DV651355.1.
            
            On Nov 15, 2007 this sequence version replaced NM_008638.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK152249.1, SRR1660819.11074.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164140, SAMN01164143
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-28                BY068806.1         5-32
            29-2019             AK159680.1         2-1992
            2020-2070           DV651355.1         374-424
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 35.94 cM"
     gene            1..2070
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /note="methylenetetrahydrofolate dehydrogenase (NAD+
                     dependent), methenyltetrahydrofolate cyclohydrolase"
                     /db_xref="GeneID:17768"
                     /db_xref="MGI:MGI:1338850"
     exon            1..176
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /inference="alignment:Splign:2.1.0"
     CDS             76..1128
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /EC_number="3.5.4.9"
                     /EC_number="1.5.1.15"
                     /note="bifunctional methylenetetrahydrofolate
                     dehydrogenase/cyclohydrolase, mitochondrial"
                     /codon_start=1
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/cyclohydrolase, mitochondrial precursor"
                     /protein_id="NP_032664.1"
                     /db_xref="CCDS:CCDS20277.1"
                     /db_xref="GeneID:17768"
                     /db_xref="MGI:MGI:1338850"
                     /translation="MASVSLLSALAVRLLRPTHGCHPRLQPFHLAAVRNEAVVISGRK
                     LAQQIKQEVQQEVEEWVASGNKRPHLSVILVGDNPASHSYVLNKTRAAAEVGINSETI
                     VKPASVSEEELLNSIRKLNNDENVDGLLVQLPLPEHIDERKVCNAVSPDKDVDGFHVI
                     NVGRMCLDQYSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGA
                     HERPGGDATVTISHRYTPKEQLKKHTILADIVISAAGIPNLITADMIKEGAAVIDVGI
                     NRVQDPVTAKPKLVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRP
                     EELEVFKSKQRGVATN"
     exon            177..361
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /inference="alignment:Splign:2.1.0"
     exon            362..484
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /inference="alignment:Splign:2.1.0"
     exon            485..637
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /inference="alignment:Splign:2.1.0"
     exon            638..745
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /inference="alignment:Splign:2.1.0"
     exon            746..838
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /inference="alignment:Splign:2.1.0"
     exon            839..964
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /inference="alignment:Splign:2.1.0"
     exon            965..2070
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2002..2007
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /note="hexamer: ATTAAA"
     polyA_site      2020
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
     regulatory      2044..2049
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /note="hexamer: AATAAA"
     polyA_site      2070
                     /gene="Mthfd2"
                     /gene_synonym="NMDMC"
                     /note="major polyA site"
ORIGIN      
        1 ggctttcgtg ggcccggggt atagcttcgt ggcccgcgcg cgcgcttctc tccggcgcgg
       61 gcaccggcgc gagccatggc ttcagtttcc ttgttgtctg cgttggctgt gcggttgttg
      121 cgccccacgc acggctgcca cccccgccta cagcccttcc acctggcggc agtgcgaaat
      181 gaagccgttg tcatttctgg aaggaaactg gcccagcaga tcaagcaaga agtacagcag
      241 gaggtggaag agtgggtggc ctcaggcaac aagcggccac acctcagtgt cattctggtt
      301 ggtgacaatc ccgccagtca ctcctatgtt ctcaacaaaa ccagggcagc agctgaagtg
      361 ggaatcaaca gtgagacaat tgtgaaacca gcctcagttt cagaggaaga gctgctgaat
      421 tcaatcagga agctgaacaa cgatgagaac gtagatggcc tccttgttca gctgccactc
      481 ccagagcaca ttgatgagag gaaggtctgc aatgccgttt ctcctgacaa ggatgttgat
      541 ggctttcatg tcattaacgt ggggcgaatg tgcttggacc agtactctat gctgccagca
      601 accccatggg gcgtgtggga gataattaag cgaacaggca ttccaacctt agggaagaat
      661 gtggtagtgg ctggcaggtc aaaaaacgtt ggaatgccga ttgcaatgtt gctgcacaca
      721 gatggagctc acgaacggcc cgggggtgat gccacagtca caatatctca tcgatacact
      781 cccaaagagc agctgaagaa gcacacgatc cttgcagaca ttgtaatatc tgccgctggc
      841 attccaaatc tgatcacagc tgacatgatc aaggaaggag cagcggtcat cgatgtggga
      901 ataaacagag ttcaagatcc tgtcactgca aagcccaaat tggttggaga tgtggatttt
      961 gaaggagtca agaagaaagc tggttatatc actcccgtcc ctggtggtgt gggtcccatg
     1021 accgtggcca tgctcatgaa gaacaccatt attgccgcaa agaaagtgct aagaccagag
     1081 gagctggaag tgttcaagtc caaacagcgc ggagtcgcca ccaactagca gcactctgag
     1141 ccagcccgag aggctaagca ggccaagagg aatgcagtgt ttttaattta ttctgtcgaa
     1201 atggtttaaa atgtttgtat ttattgaaag cttaaacggg tggctgtgtg cgcgcatggc
     1261 tctacagaac ctcaccagga tgcccatcag ttgtcctgcc tttgacctgg tgatgcacag
     1321 gagacgtcct cacaccgaga gtggatgctg aacttcatca aaaaaaaaaa aaggtctgcc
     1381 gttttaggct ctcatttccc aagtgctatt ccaatacaag ttgatgactc atcttaggtc
     1441 ccaagccttt gagttcaact ttcaaaccaa aggaaacctt agtagaggaa atgaagggaa
     1501 aggaagagaa agcagcacag ggtttcaacg gcactccctt actctctcgg gagtgcagcc
     1561 aggagtgctc cgattgccct gaggagagct cagctggctg ggcctagctg tccccctgtg
     1621 gcgggtcgtc agggaagggt tagagtctcc ccacttgctg ttatcctcct gtgagcttgt
     1681 cctatctatt ttaattttct agaatcgaaa aagctcattt tataaatgat gtgtgtatta
     1741 tcacgtttgg cttttgcagc acatttaaac ttttaagttt cagactgtta agagagttgt
     1801 gtgtctgttt tctttggcgg gtgctttgag gcctatttgt ttttgaaaaa caaaacaaaa
     1861 cttgtgcgtg ataatcacga gggcagctcg tgccaccaaa gaactgtcag cagctgcctg
     1921 cacgtctttc acgtaccccg ttggctttcc tgtaagctat ccaggagctt gggttaatat
     1981 aagacttagt cggattttct tattaaaggg ttttcttgca gttgggtttt ttgtttgttt
     2041 ttaaataaag ctcatctgtc gggtgtggaa
//
