LOCUS       NM_172308               3731 bp    mRNA    linear   ROD 23-JUL-2024
DEFINITION  Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+
            dependent) 1-like (Mthfd1l), transcript variant 2, mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_172308
VERSION     NM_172308.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ambekar,Y.S., Caiaffa,C.D., Wlodarczyk,B.J., Singh,M., Schill,A.W.,
            Steele,J.W., Zhang,J., Aglyamov,S.R., Scarcelli,G., Finnell,R.H.
            and Larin,K.V.
  TITLE     Optical coherence tomography-guided Brillouin microscopy highlights
            regional tissue stiffness differences during anterior neural tube
            closure in the Mthfd1l murine mutant
  JOURNAL   Development 151 (10) (2024)
   PUBMED   38682273
  REMARK    GeneRIF: Optical coherence tomography-guided Brillouin microscopy
            highlights regional tissue stiffness differences during anterior
            neural tube closure in the Mthfd1l murine mutant.
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 3731)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3731)
  AUTHORS   Shin,M., Vaughn,A., Momb,J. and Appling,D.R.
  TITLE     Deletion of neural tube defect-associated gene Mthfd1l causes
            reduced cranial mesenchyme density
  JOURNAL   Birth Defects Res 111 (19), 1520-1534 (2019)
   PUBMED   31518072
REFERENCE   5  (bases 1 to 3731)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3731)
  AUTHORS   Momb,J., Lewandowski,J.P., Bryant,J.D., Fitch,R., Surman,D.R.,
            Vokes,S.A. and Appling,D.R.
  TITLE     Deletion of Mthfd1l causes embryonic lethality and neural tube and
            craniofacial defects in mice
  JOURNAL   Proc Natl Acad Sci U S A 110 (2), 549-554 (2013)
   PUBMED   23267094
  REMARK    GeneRIF: Deletion of Mthfd1l causes embryonic lethality and neural
            tube and craniofacial defects in mice.
REFERENCE   7  (bases 1 to 3731)
  AUTHORS   Pike,S.T., Rajendra,R., Artzt,K. and Appling,D.R.
  TITLE     Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the
            flow of mitochondrial one-carbon units into the methyl cycle in
            embryos
  JOURNAL   J Biol Chem 285 (7), 4612-4620 (2010)
   PUBMED   19948730
  REMARK    GeneRIF: Data show that the MTHFD1L enzyme is present in
            mitochondria from normal embryonic tissues and embryonic fibroblast
            cell lines, and embryonic mitochondria possess the ability to
            synthesize formate from glycine.
REFERENCE   8  (bases 1 to 3731)
  AUTHORS   Pettitt,S.J., Liang,Q., Rairdan,X.Y., Moran,J.L., Prosser,H.M.,
            Beier,D.R., Lloyd,K.C., Bradley,A. and Skarnes,W.C.
  TITLE     Agouti C57BL/6N embryonic stem cells for mouse genetic resources
  JOURNAL   Nat Methods 6 (7), 493-495 (2009)
   PUBMED   19525957
REFERENCE   9  (bases 1 to 3731)
  AUTHORS   Lin,Q., Donahue,S.L., Moore-Jarrett,T., Cao,S., Osipovich,A.B. and
            Ruley,H.E.
  TITLE     Mutagenesis of diploid mammalian genes by gene entrapment
  JOURNAL   Nucleic Acids Res 34 (20), e139 (2006)
   PUBMED   17062627
REFERENCE   10 (bases 1 to 3731)
  AUTHORS   Tavtigian,S.V., Zabludoff,S.D. and Wold,B.J.
  TITLE     Cloning of mid-G1 serum response genes and identification of a
            subset regulated by conditional myc expression
  JOURNAL   Mol Biol Cell 5 (3), 375-388 (1994)
   PUBMED   8049528
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC153570.9, AK028211.1 and AC159148.10.
            
            On Jan 6, 2010 this sequence version replaced NM_172308.3.
            
            Transcript Variant: This variant (2) differs in the 3' UTR compared
            to variant 1. Variants 1, 2 and 3 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK038579.1, AK028211.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-50                AC153570.9         116158-116207
            51-3067             AK028211.1         2-3018
            3068-3068           AC159148.10        112405-112405
            3069-3562           AK028211.1         3020-3513
            3563-3731           AC159148.10        120315-120483
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="10"
                     /map="10 2.06 cM"
     gene            1..3731
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /note="methylenetetrahydrofolate dehydrogenase (NADP+
                     dependent) 1-like"
                     /db_xref="GeneID:270685"
                     /db_xref="MGI:MGI:1924836"
     exon            1..404
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            405..489
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            490..540
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            541..594
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            595..719
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            720..820
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            821..957
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            958..1069
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1161
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1162..1259
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1260..1433
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1434..1570
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1617
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1618..1725
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1726..1800
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1801..1903
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1904..1980
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2121
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2122..2190
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2191..2302
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2303..2442
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2484
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2485..2585
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2586..2763
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2764..2871
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2872..3024
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3025..3142
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     exon            3143..3731
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3689..3694
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /note="hexamer: AATAAA"
     polyA_site      3710
                     /gene="Mthfd1l"
                     /gene_synonym="2410004L15Rik; Fthfsdc1"
                     /note="major polyA site"
ORIGIN      
        1 ttggtacagt gccccctagg ggcctcagtt cctcccacga ggaagagtct agtctttcct
       61 gctgctggcc ctttactgtg ccgcttggct ctctggtgcc tcccacgcca gcccggggaa
      121 gaacttgcag ccgcatctcg gcgcctgggg ataaccagtc accccgcact ctgcagcagc
      181 atgagcgtcc gcctccctct cctgcttcgc cagctcggcc gccagcagct gccctcgggc
      241 ccggcgtgtc gcctccgtga gctgtgtcgc tcaggcagca gaagcagcag cagcggaggt
      301 ggtgaccccg aggggttacg cgggcggcgg ctgcaggatg gccaaacctt cagcagccat
      361 ggcccaggca atccggaagc ccccgggatg gactccatcg tcagagacgt cattcacaat
      421 tccaaggaag tgctgagctt gttgcaagaa aagaaccctg cctttaagcc agtgcttgtg
      481 gttattcagg ccggtgatga caatttgatg aaggatatga accagaattt ggccaaggag
      541 gctggtctgg acatcactca catctgcttg cctccagaca gcggagagga tgagatcata
      601 gatgaaatcc tgaagattaa tgaagacccc agagtgcatg gccttaccct tcagatctct
      661 gaagattcat tgagcaacaa agttctcaat gctttgaaac cagaaaaaga tgtggacggg
      721 gtgaccgaca tcaatctggg gaagctcgta cgaggtgacg ctccggagtg ttttgtttca
      781 cccttggcca aagctgctgt agaacttgtt gaaaagtcag ggatcacttt ggatggaaag
      841 aaggtcttgg tcgtaggggc gcacgggccc ctggaagctg ccctgcagtg gcttttccag
      901 agaaaaggat ctatgacaat gagctgcccc tgggcaacac ctcagcttcc agacaagctt
      961 cgagaggcag acattgtggt cttaggctca cctaagccag aagagattcc tgctgtgtgg
     1021 attccatcag gaaccaccat tctcaattgc ttccatgact tcttgtcagg gaagctgagc
     1081 ggaggttctc ctggggtccc cgtggacaaa ctcattgcag aagagagcgt gagtctcctt
     1141 gctgcggctc tgcgcattca gaacatggtg agcagcggca ggaggtggct cagagagcag
     1201 caacatcgaa gatggcgact gcactgcttg aaactgcagc ctctctcacc tgtgcccagt
     1261 gatattgaga tttctcgagg acagactcca aaagctgtgg atgtccttgc caaggagata
     1321 ggattgcttg cagatgaaat tgaaatctat ggcaagagca aagccaaagt ccaattgtcc
     1381 ctgctggaga ggttaaagga tcaaacagat ggaaaatacg tcttggttgc tgggatcacg
     1441 cccaccccgc tcggagaagg gaagagtaca gtcaccatcg gactagtgca ggcgctgacc
     1501 gctcacctga aagtcaactc cttcgcctgt ctgaggcagc cttctcaggg acccactttt
     1561 ggagtgaaag gaggagctgc aggaggtgga tatgctcagg tcatccccat ggaggagttc
     1621 aaccttcacc tgactgggga catccatgcc atcactgccg ctaataactt gctggccgca
     1681 gccatcgaca cgagaatttt acatgagagc actcaaacag acaaggctct ctacaatcga
     1741 ctggttccct tagtgaatgg tgtcagagaa ttttcagaaa ttcaactttc tcggctgaag
     1801 aaactgggga tacataagac tgatccaagc acactgaccg aggaggaagt gaggaagttc
     1861 gcccgcctca acattgaccc cgcaaccatc acgtggcaga gagtattgga cacgaacgac
     1921 cgatttctga gaaaaataac cattggacag ggaagtacag agaaaggata ttcccggcag
     1981 gcacagtttg acattgcagt ggccagcgag atcatggccg tgctggcctt gactgatagc
     2041 ctcacagaca tgaaagagcg gctaggaaga atggtggtgg ccagtgacaa agacgggcag
     2101 cctgtgacag ctgaggactt gggggtgacc ggcgctctga cagttttgat gaaagacgcc
     2161 atcaagccaa atctgatgca gacccttgaa ggaacacctg tgtttgtgca cgctggtccc
     2221 tttgccaaca tcgctcacgg caactcatca gtattagctg ataaaatcgc gctgaaattg
     2281 gtcggcgaag aagggtttgt ggtgactgaa gctggctttg gggctgatat tgggatggag
     2341 aaattcttca atatcaaatg ccgagcttct ggcctggtgc ccaacgttgt cgtgctggta
     2401 gccaccgttc gagctctgaa gatgcatgga ggcgggccga gtgtaactgc tggggttcct
     2461 ctcaagaaag aatatacaga ggagaacatt cagctggtag ctgatggctg ctgcaaccta
     2521 cagaagcaaa ttcagatcgc tcagctcttc ggggtgccgg tggtggtggc actgaatgtc
     2581 ttcaagacgg acacccgtgc agagattgac ttggtgtgtg agctcgctaa acgggccggt
     2641 gcctttgatg cggtcccctg ctatcactgg tcggctggag ggaaaggatc tgtggacttg
     2701 gcccgggctg tgagagaagc tgcgaataaa agaagccgct tccagttcct gtatgatgtt
     2761 cagcttccca tcgtggaaaa gataagagtc attgcccaga ctgtctatgg agccaaggac
     2821 attgagctgt ctcctgaggc gcagtccaaa atcgatcgct atacgcaaca gggttttgga
     2881 aatttgccca tttgcatggc gaagacccac ctctctctct ctcatgaacc tgacaaaaag
     2941 ggagtgccga gggatttcac tctgcccatc agtgatgtcc gggccagcat cggggctggg
     3001 ttcatttatc ctttggtggg aacgatgagc acaatgccag gactgcccac ccgaccctgc
     3061 ttttatgata tagaccttga cacagaaacg gagcaagtta aaggcttgtt ctaatggaat
     3121 gaaacccaca ggactgggca agaaaacagc tcacacaaga atcccagcct ttaaaaccac
     3181 aacacaagta agagtttaac accagaggaa aagacgactt ggctctgaga ccccaccccc
     3241 tctttttcag agtcttcaaa caaaaaacgt ctccttgcct ttttgctgca gttggagaag
     3301 aaaatgaatt ttgaaatctt gtgatgcaat gctggagcaa tcctgagaca ggccaaagac
     3361 cgcttcctca tacaagatta actctgtttc cagccaagtg gtgttcaaca gaaggacctg
     3421 cgccacatct ggaaggagtg aagttactct tggttccgat agtgtttaca ccatttctgc
     3481 tgcttatact ttagaaagtt tattttatgg ggactagggc tttgcaagag tgtgaactaa
     3541 aagctaatgc ttccgtcttt ggagttttct ctttcatctt ttttattctt cctttcctca
     3601 cctgtgttga ggatgggatg tcaggcctct gcttgcaccg tccagcgctg tgctgccaaa
     3661 ctgtatttgt ttttgtcttt gaactgaaaa taaacatgtg ttctagaaaa ccgtgttcag
     3721 ctagtaaagc c
//
