LOCUS       NM_138745               3324 bp    mRNA    linear   ROD 31-DEC-2024
DEFINITION  Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+
            dependent), methenyltetrahydrofolate cyclohydrolase,
            formyltetrahydrofolate synthase (Mthfd1), mRNA.
ACCESSION   NM_138745
VERSION     NM_138745.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Guan,J., Li,M., Wang,Y., Zhang,Y., Que,Y., Lu,S., Wang,J., Zhu,J.,
            Huang,J., Zhen,Z., Sun,F., Song,M. and Zhang,Y.
  TITLE     MTHFD1 regulates the NADPH redox homeostasis in MYCN-amplified
            neuroblastoma
  JOURNAL   Cell Death Dis 15 (2), 124 (2024)
   PUBMED   38336749
  REMARK    GeneRIF: MTHFD1 regulates the NADPH redox homeostasis in
            MYCN-amplified neuroblastoma.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3324)
  AUTHORS   Imbard,A., Schwendimann,L., Lebon,S., Gressens,P., Blom,H.J. and
            Benoist,J.F.
  TITLE     Liver and brain differential expression of one-carbon metabolism
            genes during ontogenesis
  JOURNAL   Sci Rep 11 (1), 21132 (2021)
   PUBMED   34702858
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3324)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3324)
  AUTHORS   MacFarlane,A.J., Perry,C.A., Girnary,H.H., Gao,D., Allen,R.H.,
            Stabler,S.P., Shane,B. and Stover,P.J.
  TITLE     Mthfd1 is an essential gene in mice and alters biomarkers of
            impaired one-carbon metabolism
  JOURNAL   J Biol Chem 284 (3), 1533-1539 (2009)
   PUBMED   19033438
  REMARK    GeneRIF: Mthfd1 is an essential gene in mice and alters biomarkers
            of impaired one-carbon metabolism.
REFERENCE   6  (bases 1 to 3324)
  AUTHORS   Patel,H., Di Pietro,E., Mejia,N. and MacKenzie,R.E.
  TITLE     NAD- and NADP-dependent mitochondrially targeted
            methylenetetrahydrofolate dehydrogenase-cyclohydrolases can rescue
            mthfd2 null fibroblasts
  JOURNAL   Arch Biochem Biophys 442 (1), 133-139 (2005)
   PUBMED   16150419
REFERENCE   7  (bases 1 to 3324)
  AUTHORS   Christensen,K.E., Patel,H., Kuzmanov,U., Mejia,N.R. and
            MacKenzie,R.E.
  TITLE     Disruption of the mthfd1 gene reveals a monofunctional
            10-formyltetrahydrofolate synthetase in mammalian mitochondria
  JOURNAL   J Biol Chem 280 (9), 7597-7602 (2005)
   PUBMED   15611115
  REMARK    GeneRIF: MTHFD1 has a role in production of monofunctional
            10-formyltetrahydrofolate synthetase in mammalian mitochondria
REFERENCE   8  (bases 1 to 3324)
  AUTHORS   Patel,H., Christensen,K.E., Mejia,N. and MacKenzie,R.E.
  TITLE     Mammalian mitochondrial methylenetetrahydrofolate
            dehydrogenase-cyclohydrolase derived from a trifunctional
            methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase
  JOURNAL   Arch Biochem Biophys 403 (1), 145-148 (2002)
   PUBMED   12061812
  REMARK    GeneRIF: Mammalian mitochondrial methylenetetrahydrofolate
            dehydrogenase-cyclohydrolase derived from a trifunctional
            methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase.
REFERENCE   9  (bases 1 to 3324)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   10 (bases 1 to 3324)
  AUTHORS   Peri,K.G. and MacKenzie,R.E.
  TITLE     Transcriptional regulation of murine NADP(+)-dependent
            methylenetetrahydrofolate dehydrogenase-cyclohydrolase-synthetase
  JOURNAL   FEBS Lett 294 (1-2), 113-115 (1991)
   PUBMED   1720740
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CD545120.1, AK159812.1,
            AK042958.1 and AW536566.1.
            
            On Oct 23, 2009 this sequence version replaced NM_138745.1.
            
            Summary: This gene encodes a trifunctional cytoplasmic enzyme. The
            encoded protein functions as a methylenetetrahydrofolate
            dehydrogenase, a methenyltetrahydrofolate cyclohydrolase, and a
            formyltetrahydrofolate synthase. The encoded enzyme functions in de
            novo synthesis of purines and thymidylate and in regeneration of
            methionine from homocysteine. [provided by RefSeq, Oct 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK129012.1, SRR1660815.187204.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-389               CD545120.1         1-389
            390-2044            AK159812.1         202-1856
            2045-2270           AK042958.1         1881-2106
            2271-3306           AK159812.1         2083-3118
            3307-3324           AW536566.1         1-18                c
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 33.52 cM"
     gene            1..3324
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /note="methylenetetrahydrofolate dehydrogenase (NADP+
                     dependent), methenyltetrahydrofolate cyclohydrolase,
                     formyltetrahydrofolate synthase"
                     /db_xref="GeneID:108156"
                     /db_xref="MGI:MGI:1342005"
     exon            1..313
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     CDS             273..3080
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /EC_number="1.5.1.5"
                     /EC_number="6.3.4.3"
                     /EC_number="3.5.4.9"
                     /note="C1-tetrahydrofolate synthase; C1-THF synthase"
                     /codon_start=1
                     /product="C-1-tetrahydrofolate synthase, cytoplasmic"
                     /protein_id="NP_620084.2"
                     /db_xref="CCDS:CCDS25990.1"
                     /db_xref="GeneID:108156"
                     /db_xref="MGI:MGI:1342005"
                     /translation="MAPAGILNGKLVSAQIRDRLKNQVTRMQEQVPGFTPGLAILQVG
                     DRDDSNLYINVKLKAAEEIGIKATHIKLPRTSTESEVLKYVISLNEDASVHGFIVQLP
                     LDSENSINTEAVINAIAPEKDVDGLTSVSAGKLARGDLNDCFIPCTPKGCLELIKEAG
                     VQIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTANLDKEVNKGDILVVATGQ
                     PEMVKGEWIKPGAVVIDCGINYVPDDTKPNGRKVVGDVAYDEAKERASFITPVPGGVG
                     PMTVAMLMQSTVESAQRFLQKFKPGKWTIQYNKLNLKTPVPSDIAISRSCKPKLIGNL
                     AREIGLLTEEVELYGETKAKVLLSALDRLKHQPDGKYVVVTGITPTPLGEGKSTTTIG
                     LVQALGAHLRQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAIT
                     AANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGIRKFSDIQIRRLRRLGIEKTDPT
                     TLTDDEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQSPTEKGHTRTAQFDISVA
                     SEIMAVLALTSSLEDMRERLGRMVVASSKKGEPISCEDLGVSGALTVLMKDAIKPNLM
                     QTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTEAGFGADIGMEKFFN
                     IKCRYSGLQPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYTEEDLDLVEKGFSNLRK
                     QIENARMFGVPVVVAVNVFKTDTDAELDLVSRLSREHGAFDAVKCTHWAEGGQGALAL
                     AQAVQRASQAPSSFQLLYDLKLSIEDKIRIIAQRIYGADDIELLPEAQNKAEIYTKQG
                     FGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGTMSTMPGLP
                     TRPCFYDIDLDPETEQVNGLF"
     exon            314..398
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            399..458
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            459..512
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            513..649
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            650..750
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            751..887
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            888..999
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1127
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1225
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1226..1399
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1536
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1583
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1691
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1766
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1767..1869
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1870..1946
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            1947..2087
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            2088..2156
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            2157..2268
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            2269..2408
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            2409..2450
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2551
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            2552..2729
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            2730..2837
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            2838..2990
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            2991..3084
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     exon            3085..3324
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3286..3291
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /note="hexamer: AATAAA"
     polyA_site      3307
                     /gene="Mthfd1"
                     /gene_synonym="Dcs; E430024A07Rik; Mthfd"
                     /note="major polyA site"
ORIGIN      
        1 ccagtcattg ccgccaacca gtctttggtg acaaccctct tgctgcctgg gcccaccggc
       61 tcccgcagac gctcctgggc ctcctctgtc agcgggtcac gacgtctttc cgggtcgcct
      121 gcgcaccttc gctgctgagc gcgacgcagg cgcactagct gatttctccc gggaggtccc
      181 tgagcacgtg ggttgtgctg tcctgcgcgc gcggagcgac tggaacctga tttttgctgg
      241 tgttcaccgt gggtggcaga ctagcaaagg ccatggcacc agcgggaatc ctgaacggga
      301 aactggtttc tgcgcaaatc agggatcgac tgaagaacca ggtaactcgg atgcaggagc
      361 aggtacctgg cttcacccct ggcctggcaa tcctgcaggt tggcgacaga gatgattcca
      421 atctttatat aaatgtgaag ctgaaggctg ctgaagagat tgggatcaaa gccactcaca
      481 ttaaattacc aagaacatcc acagagtccg aggtgttaaa atatgtcatc tccctgaacg
      541 aggatgcctc tgtgcacgga ttcatagtgc agctgccttt agattcagag aattccatta
      601 acactgaggc agtcatcaat gccattgcac ccgagaagga tgtggatggg ttgacgagcg
      661 tcagtgctgg gaagctcgcc agaggtgatc taaatgactg cttcatccca tgcacaccca
      721 agggatgctt ggaactcatc aaagaggcag gggtgcagat tgccggaagg cacgcggtgg
      781 tggttggtcg aagtaagata gttggtgcac ccatgcatga cctccttctg tggaacaatg
      841 ccacggtgac cacctgccac tccaagactg ccaatctgga caaggaggta aataaaggag
      901 acattctggt ggttgccaca ggccagcctg agatggtgaa gggggagtgg atcaagcctg
      961 gggctgtggt catagactgt gggatcaatt atgttccaga tgatacaaaa ccaaatggaa
     1021 ggaaagtcgt gggtgatgtg gcctacgatg aggccaagga gagggcgagc tttatcacgc
     1081 ctgtccctgg tggcgtgggg cccatgaccg tggcaatgct gatgcagagc acagtagaga
     1141 gcgcacagcg cttcctacag aaatttaagc cagggaagtg gacaattcag tataacaagc
     1201 tgaacctcaa gactcctgtc ccaagtgaca ttgctatatc acgatcttgc aaaccaaagc
     1261 tcatcgggaa cctggcccga gaaatcgggc tgctcaccga agaggtggag ttgtacgggg
     1321 aaacgaaggc taaagtcttg ctgtcagcat tagatcgcct gaagcatcag ccagatggga
     1381 aatatgtcgt ggtgactgga attactccaa caccgctggg agaagggaag agtaccacca
     1441 cgatcgggct ggtgcaggcc ctcggcgccc acctgcgaca gaacgtcttt gcatgtgtgc
     1501 gacagccttc gcagggcccc acctttggga taaaaggtgg cgctgcagga ggcggctatt
     1561 cccaggtcat tcctatggaa gagtttaacc tccacctcac tggtgacatc cacgccatca
     1621 ctgctgctaa caacctcgtg gctgctgcca ttgatgctcg gatatttcat gagctgaccc
     1681 agacagacaa ggctctcttt aatcgtctgg taccttcagt aaacggaata agaaaattct
     1741 ctgacatcca aatccgcagg ttacggaggc taggcattga gaagactgac cccaccacgc
     1801 tgacagatga cgagataaac agatttgcaa gactggatat tgacccagaa accataacat
     1861 ggcagagagt gttggacacc aacgatagat tcctgaggaa gatcacaatt ggacagtctc
     1921 caacagagaa aggccacaca cgcacggccc agtttgatat ctctgtggcc agtgagatca
     1981 tggcagtcct ggccctcacg agctctctgg aagacatgag agagagactg ggcagaatgg
     2041 tggtagcgtc cagtaagaaa ggggagccta tcagttgcga agacctgggc gtgagtgggg
     2101 cgctgacggt gctgatgaag gatgcaatca agcccaacct catgcagacc ttagagggca
     2161 cgccagtgtt tgtccatgcc gggcctttcg ccaacattgc acacgggaat tcctccatca
     2221 ttgcagaccg gattgcactc aagctggtag gccctgaggg cttcgtagta acggaagctg
     2281 gattcggagc agacatagga atggaaaagt tcttcaacat caagtgccgg tattctggtc
     2341 tccagccaca cgtggtggtt ctggttgcca ctgtcagggc ccttaagatg cacgggggtg
     2401 gccccacggt caccgctgga ctgcctcttc ccaaggctta cacagaagag gacctggacc
     2461 tggttgaaaa gggcttcagt aacttgagga aacaaatcga aaatgctaga atgtttggag
     2521 tccctgtcgt cgtggctgtg aacgtgttca agacagatac agatgctgag ctggaccttg
     2581 tcagccgcct ctccagagaa cacggggctt ttgatgctgt caagtgcacc cactgggcag
     2641 aagggggcca gggggccttg gccctggctc aggctgtcca gagagcgtca caggccccca
     2701 gcagcttcca gctcctctat gacctcaagc tctcaattga ggataaaatc aggattattg
     2761 cacagaggat ctacggggcc gatgacatcg aattgctccc ggaagcacag aacaaagcag
     2821 aaatttacac aaagcagggc tttgggaatc tacccatctg catggccaaa acacacttgt
     2881 ccttgtctca caacccagag caaaaagggg tgcctactgg tttcgttctg cccatccggg
     2941 acatccgtgc cagcgttggg gcaggtttcc tgtatccttt agtaggaacg atgagcacaa
     3001 tgcctggact ccctacgcgg ccctgttttt atgatattga tttggatcct gaaacggaac
     3061 aagtgaatgg attgttttaa gcagatcttc catctccaag aggccactct gtccggccag
     3121 tgtctgttca ggcccactga agaagtgtgc agaagtcttg gaagtctgtc cccgccctga
     3181 agagcttcag aaatagtggg agtttcccta aagcctttca tagccttaat tcatgtcatg
     3241 tataaattaa cataaatcat gtctatttac atagtgaagg tccagaataa atgaaataat
     3301 tttgctacct tggtgttgaa acat
//
