LOCUS       NM_027134               2042 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus mitochondrial methionyl-tRNA formyltransferase
            (Mtfmt), mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_027134
VERSION     NM_027134.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Sun,X., Liu,S., Cai,J., Yang,M., Li,C., Tan,M. and He,B.
  TITLE     Mitochondrial Methionyl-tRNA Formyltransferase Deficiency
            Alleviates Metaflammation by Modulating Mitochondrial Activity in
            Mice
  JOURNAL   Int J Mol Sci 24 (6), 5999 (2023)
   PUBMED   36983072
  REMARK    GeneRIF: Mitochondrial Methionyl-tRNA Formyltransferase Deficiency
            Alleviates Metaflammation by Modulating Mitochondrial Activity in
            Mice.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2042)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2042)
  AUTHORS   Arguello,T., Kohrer,C., RajBhandary,U.L. and Moraes,C.T.
  TITLE     Mitochondrial methionyl N-formylation affects steady-state levels
            of oxidative phosphorylation complexes and their organization into
            supercomplexes
  JOURNAL   J Biol Chem 293 (39), 15021-15032 (2018)
   PUBMED   30087118
  REMARK    GeneRIF: Here, we generated a Mtfmt-KO mouse fibroblast cell line
            and demonstrated that N-formylation of the first methionine via
            fMet-tRNA(Met) by MTFMT is not an absolute requirement for
            initiation of protein synthesis.
REFERENCE   5  (bases 1 to 2042)
  AUTHORS   Arguello,T. and Moraes,C.T.
  TITLE     Cre recombinase activity is inhibited in vivo but not ex vivo by a
            mutation in the asymmetric spacer region of the distal loxP site
  JOURNAL   Genesis 53 (11), 695-700 (2015)
   PUBMED   26331883
REFERENCE   6  (bases 1 to 2042)
  AUTHORS   Yokoyama,S., Ito,Y., Ueno-Kudoh,H., Shimizu,H., Uchibe,K.,
            Albini,S., Mitsuoka,K., Miyaki,S., Kiso,M., Nagai,A., Hikata,T.,
            Osada,T., Fukuda,N., Yamashita,S., Harada,D., Mezzano,V., Kasai,M.,
            Puri,P.L., Hayashizaki,Y., Okado,H., Hashimoto,M. and Asahara,H.
  TITLE     A systems approach reveals that the myogenesis genome network is
            regulated by the transcriptional repressor RP58
  JOURNAL   Dev Cell 17 (6), 836-848 (2009)
   PUBMED   20059953
REFERENCE   7  (bases 1 to 2042)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK009430.1 and AK133492.1.
            
            On Apr 25, 2009 this sequence version replaced NM_027134.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK133492.1, SRR1660819.74034.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AK009430.1         2-7
            7-672               AK133492.1         2-667
            673-1390            AK009430.1         674-1391
            1391-2042           AK133492.1         1386-2037
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 35.31 cM"
     gene            1..2042
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /note="mitochondrial methionyl-tRNA formyltransferase"
                     /db_xref="GeneID:69606"
                     /db_xref="MGI:MGI:1916856"
     exon            1..226
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             33..1193
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /EC_number="2.1.2.9"
                     /codon_start=1
                     /product="methionyl-tRNA formyltransferase, mitochondrial"
                     /protein_id="NP_081410.2"
                     /db_xref="CCDS:CCDS23292.1"
                     /db_xref="GeneID:69606"
                     /db_xref="MGI:MGI:1916856"
                     /translation="MLLPRCCWGRWLMGRRPRCSCQAPAGFDGKDGRGSRVREKPPWR
                     VLFLGTDHFARETLRALHAARDGKEEKLIEKLEVVTVPSLSPKGLPVKQYAIQSQLPV
                     YEWPDVGSGEYDVGVVASFGRLLSEALILKFPYGILNVHPSCLPRWRGPAPIIHTVLH
                     GDTVTGVTIMQIRPKRFDIGPILQQETIPVPPKSTSKELEAVLSKLGANMLISVLKNL
                     PESLNNGRPQPAEGVTYAPKVSAGTSCVKWEEQTSEQVLRLHLAIGDIVPLQTLWMEN
                     TVKLLDLVEVNNSILADPKLTGQTVTPGFVVYHRPSQMLLVRCKDGWIGVRSVMLKKT
                     LTATDFYNGYLHAWYQKNSHAHPSQCRFQTLRLPTKMQQKTKLLLCNSALSS"
     exon            227..436
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            437..559
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            560..662
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            663..738
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            739..830
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            831..909
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            910..992
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            993..2042
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1373..1378
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1405
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /note="major polyA site"
     regulatory      1435..1440
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
                     /note="hexamer: ACTAAA"
     polyA_site      1461
                     /gene="Mtfmt"
                     /gene_synonym="2310020P08Rik"
ORIGIN      
        1 atgcgcatgg tgggccgctg cgggccgagg tgatgctcct tccgcggtgc tgctggggtc
       61 ggtggctgat gggaaggagg ccacgctgtt cctgtcaagc acctgccggg ttcgacggga
      121 aggacggccg aggttcccga gtccgcgaga agccgccttg gcgggtgctg ttcctcggca
      181 ccgaccactt tgcccgggaa acgctgcggg cgctgcacgc cgccagagac ggcaaagagg
      241 aaaagttaat tgagaaactg gaggttgtca cagtgccttc gctatccccc aaaggactac
      301 ctgtgaagca atatgctatt cagtctcagc tcccggtgta cgagtggccc gacgtgggat
      361 ctggagaata tgatgttggg gtagtggctt cctttggccg gcttttgagt gaggctctta
      421 ttcttaaatt tccctacggc atactgaatg tgcaccccag ttgcctcccg aggtggcgtg
      481 gtcccgctcc gataatccac acggtgcttc atggagacac tgtcactggc gtaacgatta
      541 tgcaaattag acccaaaaga tttgatatag gcccaattct ccagcaggaa accatccctg
      601 tgcctcccaa gagcacatca aaggaattgg aagcagtgtt gtcaaaactt ggtgccaaca
      661 tgcttatctc agttttgaaa aatctgcccg aaagtctgaa caatggaagg ccgcagccag
      721 ccgagggagt gacatatgcc cccaaggtct ctgctggcac cagctgtgtc aagtgggagg
      781 agcaaacctc agagcaagta ctcagacttc accttgccat tggggatata gtcccactgc
      841 agacgctctg gatggagaac accgttaaac ttctggatct cgtagaagtt aacaattcca
      901 tccttgcaga tccgaaatta acaggacaaa ctgtaactcc ggggttcgtg gtgtaccaca
      961 gaccatcgca aatgctgctg gttcgctgta aggatggctg gatcggtgtt cggtcagtaa
     1021 tgcttaagaa aacactaaca gctactgatt tctacaatgg atatttgcat gcgtggtacc
     1081 agaaaaattc ccacgctcac ccaagccagt gcagatttca gactctcaga cttccaacaa
     1141 agatgcagca gaaaacaaag ttgttgctat gcaacagtgc attaagtagc tagaaagaca
     1201 acggacaagg acctattaca cacttgtaat ttattaaaag cttgatttac aagcaagaag
     1261 atgacagcgt gggagaaaga gaagtggttg tcaggaggcg gaagtctttc gctctccatt
     1321 gtgtgtagca gtcaagggcg gacttttcta cttgaatcac acaagaagtt taaataaaat
     1381 ttgtatttat aatgttcagc ttaaaggagc ttgtatcctt tgaagagtaa gacaactaaa
     1441 tggtgtgcat atattctaaa aggttgtttt aaatggtgca tctatggact tcgggcctag
     1501 ctcagtaggt agagtgcctg cttagccatg gtgaagttct gagttcgatc cccagcacct
     1561 cataatccag atagtggtgc acacctgtag tcccagcact cagcagaagc aggaacgtca
     1621 gaagttgaag ggcatcctca gctgtccgcg agttctgtag gacattccaa tttggatttt
     1681 tattattgct ggaaaaaagt ttgctttacc ccagacttga aactggactc cggcttgggg
     1741 tggggctcag ttggtagaat acttgcctag tgtgtacaca gtgctgtagt cccagtactt
     1801 ggaagatgaa ggcgggagga tcagttcaag gcatcactac caagcatgtt taaggcctgc
     1861 ccagggctgg agagagggct cagcggttaa gaacactgac tgctcttcca gaggtcctga
     1921 gttcaattcc cagcaaccac atggtggctc acaaccatct ggaatgagat ctgatgcctt
     1981 cttctggtgt gtctgaagac agctacagtg tactcacata taataataaa tctttaaaaa
     2041 ac
//
