LOCUS       NM_008636               7631 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus metal response element binding transcription factor 1
            (Mtf1), mRNA.
ACCESSION   NM_008636 XM_207848
VERSION     NM_008636.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Chee,J.M., Lanoue,L., Clary,D., Higgins,K., Bower,L., Flenniken,A.,
            Guo,R., Adams,D.J., Bosch,F., Braun,R.E., Brown,S.D.M., Chin,H.G.,
            Dickinson,M.E., Hsu,C.W., Dobbie,M., Gao,X., Galande,S.,
            Grobler,A., Heaney,J.D., Herault,Y., de Angelis,M.H., Mammano,F.,
            Nutter,L.M.J., Parkinson,H., Qin,C., Shiroishi,T., Sedlacek,R.,
            Seong,J.K., Xu,Y., Brooks,B., McKerlie,C., Lloyd,K.C.K.,
            Westerberg,H. and Moshiri,A.
  CONSRTM   International Mouse Phenotyping Consortium
  TITLE     Genome-wide screening reveals the genetic basis of mammalian
            embryonic eye development
  JOURNAL   BMC Biol 21 (1), 22 (2023)
   PUBMED   36737727
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 7631)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   4  (bases 1 to 7631)
  AUTHORS   Lee,M., Won,Y., Shin,Y., Kim,J.H. and Chun,J.S.
  TITLE     Reciprocal activation of hypoxia-inducible factor (HIF)-2alpha and
            the zinc-ZIP8-MTF1 axis amplifies catabolic signaling in
            osteoarthritis
  JOURNAL   Osteoarthritis Cartilage 24 (1), 134-145 (2016)
   PUBMED   26241779
  REMARK    GeneRIF: Findings identify a reciprocal activation mechanism
            involving HIF-2alpha and the zinc-ZIP8-MTF1 axis during
            osteoarthritis pathogenesis that amplifies catabolic signaling and
            cartilage destruction.
REFERENCE   5  (bases 1 to 7631)
  AUTHORS   van Loo,K.M., Schaub,C., Pitsch,J., Kulbida,R., Opitz,T.,
            Ekstein,D., Dalal,A., Urbach,H., Beck,H., Yaari,Y., Schoch,S. and
            Becker,A.J.
  TITLE     Zinc regulates a key transcriptional pathway for epileptogenesis
            via metal-regulatory transcription factor 1
  JOURNAL   Nat Commun 6, 8688 (2015)
   PUBMED   26498180
  REMARK    GeneRIF: MTF1 mediates the increase of CaV3.2 mRNA and a rise in
            intracellular Zn(2+) which is associated with status epilepticus.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7631)
  AUTHORS   Dalton,T.P., Li,Q., Bittel,D., Liang,L. and Andrews,G.K.
  TITLE     Oxidative stress activates metal-responsive transcription factor-1
            binding activity. Occupancy in vivo of metal response elements in
            the metallothionein-I gene promoter
  JOURNAL   J Biol Chem 271 (42), 26233-26241 (1996)
   PUBMED   8824273
REFERENCE   7  (bases 1 to 7631)
  AUTHORS   Muller,H.P., Brungnera,E., Georgiev,O., Badzong,M., Muller,K.H. and
            Schaffner,W.
  TITLE     Analysis of the heavy metal-responsive transcription factor MTF-1
            from human and mouse
  JOURNAL   Somat Cell Mol Genet 21 (5), 289-297 (1995)
   PUBMED   8619126
REFERENCE   8  (bases 1 to 7631)
  AUTHORS   Radtke,F., Georgiev,O., Muller,H.P., Brugnera,E. and Schaffner,W.
  TITLE     Functional domains of the heavy metal-responsive transcription
            regulator MTF-1
  JOURNAL   Nucleic Acids Res 23 (12), 2277-2286 (1995)
   PUBMED   7610056
REFERENCE   9  (bases 1 to 7631)
  AUTHORS   Heuchel,R., Radtke,F., Georgiev,O., Stark,G., Aguet,M. and
            Schaffner,W.
  TITLE     The transcription factor MTF-1 is essential for basal and heavy
            metal-induced metallothionein gene expression
  JOURNAL   EMBO J 13 (12), 2870-2875 (1994)
   PUBMED   8026472
REFERENCE   10 (bases 1 to 7631)
  AUTHORS   Radtke,F., Heuchel,R., Georgiev,O., Hergersberg,M., Gariglio,M.,
            Dembic,Z. and Schaffner,W.
  TITLE     Cloned transcription factor MTF-1 activates the mouse
            metallothionein I promoter
  JOURNAL   EMBO J 12 (4), 1355-1362 (1993)
   PUBMED   8467794
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DV658365.1, AK150295.1, AK030284.1, AK036233.1, AK030868.1,
            CK380917.1, CF354134.1 and AK021268.1.
            
            On May 17, 2008 this sequence version replaced NM_008636.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK030284.1, X71327.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-735               DV658365.1         20-754
            736-1181            AK150295.1         798-1243
            1182-2617           AK030284.1         1161-2596
            2618-6519           AK036233.1         323-4224
            6520-7215           AK030868.1         2163-2858
            7216-7539           CK380917.1         103-426             c
            7540-7545           CF354134.1         94-99               c
            7546-7631           AK021268.1         531-616             c
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 57.91 cM"
     gene            1..7631
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /note="metal response element binding transcription factor
                     1"
                     /db_xref="GeneID:17764"
                     /db_xref="MGI:MGI:101786"
     exon            1..95
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     exon            96..551
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..2174
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /note="transcription factor MTF-1; MRE-binding
                     transcription factor; metalloregulatory transcription
                     factor"
                     /codon_start=1
                     /product="metal regulatory transcription factor 1"
                     /protein_id="NP_032662.3"
                     /db_xref="CCDS:CCDS18627.1"
                     /db_xref="GeneID:17764"
                     /db_xref="MGI:MGI:101786"
                     /translation="MGEHSPDDNIIFFKGEEDDLTPHDKMLRFVDDNGLVPSSSGTVY
                     DRTTVLIEQDPGTLEDDEDDGQCGEPLPFLVEGEEGFLIDQEAMSQGYVQHIISPDQI
                     HLTINPGSTPMPRNIEGATLTLQSECPETKRKEVKRYQCTFEGCPRTYSTAGNLRTHQ
                     KTHRGEYTFVCNQEGCGKAFLTSYSLRIHVRVHTKEKPFECDVQGCEKAFNTLYRLKA
                     HQRLHTGKTFNCESQGCSKYFTTLSDLRKHIRTHTGEKPFRCDHDGCGKAFAASHHLK
                     THVRTHTGERPFFCPSNGCEKTFSTQYSLKSHMKGHDNKGTAYSALPQHNGSEDTNHS
                     LYLSELGLLSTDSELQENSSSTQDQDLSTISPAIIFESMFQNSDDPGIQDDPLQTAAL
                     IDSFNGDAESVIDVPPPAGNSASLSLPLVLQSGISEPPQPLLPATAPSAPPPAPSLGP
                     GSQPAAFGSPPALLQPPEVPVPHSTQFAANHQEFLPHPQAPPQTIVPGLSVVAGAPAS
                     AATVASAVAAPAPPQSTTEPLPAMVQTLPLGANSVLTNNPTITITPTPNTAILQSSLV
                     MGEQNLQWILNGATSSPQNQEQIQQASKVEQVYFATAVPVASGTGSSVQQIGLSVPVI
                     IIKQEEACQCQCACRDSAKERAAGRRKGCSSPPPPEPNPQPPDGPSLQLPP"
     exon            552..790
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     exon            791..922
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     exon            923..996
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1133
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     exon            1134..1211
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1314
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     exon            1315..1913
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     exon            1914..1977
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     exon            1978..7631
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2719..2724
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /note="hexamer: AATGAA"
     polyA_site      2725
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /note="major polyA site"
     regulatory      7193..7198
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
                     /note="hexamer: ATTAAA"
     polyA_site      7217
                     /gene="Mtf1"
                     /gene_synonym="MTF-1; Thyls"
ORIGIN      
        1 gaagcggaag tgacgctagg gacaggtggg ggcaatcatg gtgccgcttg ggaggggaga
       61 agctgctgct gccgccgttg ccgggagccg cggagaaagg ccgttacctt tccatttctc
      121 acaattgggc tgagcacacg tgagccatgg gggaacacag tccagacgac aatatcatct
      181 tctttaaggg agaggaggat gacctaaccc cgcatgacaa gatgctcagg tttgtggatg
      241 acaacggact ggtgccttcc tcatctggaa ctgtttatga taggaccact gttctcatag
      301 aacaggaccc tggcactttg gaggatgatg aagacgatgg acagtgtgga gaacccttgc
      361 cttttctggt ggaaggtgaa gagggcttct taatagatca ggaagcaatg tcccagggtt
      421 acgtacaaca cattatctca ccagatcaga ttcatttgac tataaaccct ggttccacac
      481 ccatgccaag aaacattgaa ggtgcaactc ttactctgca gtcggaatgt ccagaaacga
      541 aacggaaaga agtaaagcgg taccagtgca cctttgaggg atgccctcgc acctacagca
      601 cagcaggcaa cctgcgcacc caccagaaga ctcaccgagg agagtacacc ttcgtctgta
      661 atcaggaggg ctgtggcaag gccttcctca cctcctacag cctcaggatc catgtgcgag
      721 tgcacacaaa ggagaagcca tttgagtgtg acgtgcaggg ctgtgagaag gcgttcaaca
      781 cactgtacag gttgaaagca catcagaggc ttcacacagg gaaaacgttt aactgtgaat
      841 ctcaaggctg cagcaaatac ttcaccacac tcagtgacct gcggaagcac attcggaccc
      901 atacaggaga aaagccattt cgatgtgatc acgacggctg tgggaaagcg tttgcagcga
      961 gccaccacct taaaactcac gtccggacgc acactggtga aagacccttc ttctgcccca
     1021 gcaacggctg tgagaagacg tttagcactc agtacagtct caaaagtcac atgaaaggtc
     1081 atgataacaa aggcactgca tacagtgcac ttccacagca caatggatca gaggatacaa
     1141 atcactcact ttatctgagt gagctgggcc ttctgtccac agattctgaa ctgcaagaaa
     1201 attccagttc gacacaggac caggacctca gcacaatttc accagcaatc atctttgaat
     1261 caatgttcca gaattccgac gaccctggga ttcaggacga ccctctgcag acagctgcct
     1321 tgattgacag ttttaatggt gatgcagagt ccgtcattga tgtcccaccg cctgcaggaa
     1381 attcggcgtc tttatctctt ccactcgtac tgcagtctgg catctccgag ccaccccagc
     1441 ctctgctacc agcaacagct ccgtccgctc ctccacctgc tccctcccta ggacctggtt
     1501 cccagccagc tgcatttggc agcccccctg ctctcttaca acctccagaa gtgcctgttc
     1561 cccacagcac acagtttgct gctaatcatc aagagtttct tccgcacccc caggcgccac
     1621 cccagaccat cgtaccagga ctttctgttg ttgctggggc tcctgcatca gcagcaacag
     1681 tggcgtcagc cgtggcagca ccagccccac cacaaagtac tactgagccc ctgcctgcta
     1741 tggtccagac tctgcccctg ggtgccaact ctgtcctaac taataatccc accataacca
     1801 tcaccccaac tcctaacacg gcaatcctgc agtccagcct agtcatggga gaacagaact
     1861 tacagtggat attgaacggt gccaccagtt caccacaaaa ccaagaacaa attcagcaag
     1921 catcgaaagt ggagcaggtg tacttcgcca ccgctgtacc agtggccagt ggcacaggga
     1981 gctctgttca gcagattggc ctcagtgttc ctgtgatcat catcaaacaa gaggaggcct
     2041 gtcagtgtca gtgcgcgtgc cgggactctg cgaaggagcg ggcggccggc cggagaaagg
     2101 gatgttcttc cccaccccct ccagagccga acccccagcc tcctgatggg cccagcctgc
     2161 agctgccacc ctagacttct ccctcatccc tgcccccccc ccccccctcc gctggaccct
     2221 cttcctgtga gcacagcaga caggctgagg ctcctcagac cctccgaaac gttaagtgcc
     2281 atggatgtgt cagagttcct gtccctccag agcctggaca ccccgtccaa tccggttcac
     2341 agtgaagcac tactgcaggg gtagaggaaa gagcctggcc ggcagcttct ccaggggaag
     2401 ggcctgtgcg cacacccctc caggaacagg gtgagcagga agtaccggct gtgatgctgc
     2461 cgtcatgggg tcagaaattg gaaggatgaa gaaatctgcc atctgaaagc tcacctttca
     2521 gacgtatttt ctttactcgt atcccaggaa catccatttt aaggaactga tctttggagg
     2581 aaaaaacaaa aaacaaaaac aaaaaaagaa aaaaaaaagc taagttataa gtgaactgtc
     2641 tggctgcact gtgtgtcact tttgcttatt gttatgtgaa cttggaaact aaggttacgt
     2701 gtatgcataa aagttctaaa tgaaagggtg tggtttccat cactttggta ctgcccatca
     2761 tttgcactgg ggtcactgtg gattgggcag gagaggccac tgtgcctgcc gggtgttgct
     2821 tctcttctgt gtctgtttaa tccgaggcag tacctggagg gccagaccac cgctctatga
     2881 aagcggggag tggcaagggc aggcgtgagt tagactgggt gagttgcttt gttgttggca
     2941 cttggtttct gtggagcttg gggtagatgc agagggggct gccctgtgtc ctgcttagtc
     3001 ctagcgggca gctgcaggcc tcctggccag agggaagatg tggttctgca gggccccgag
     3061 gcagttgttg acagctctgt tgataatgtg ctagacccta gagctatcta gcacagccac
     3121 agtcttgcct tcttggctct ttccatctct cagtgcttgt tagtgcttga ggcttgaagt
     3181 tcatctctcc tacggaagcg ccctgtttta tcctaaagtg aatgagaggc ttctggccag
     3241 tggctgccag ggtcttcctg ccttcgatga tgtttgcttc ctcagagcag aagggctgct
     3301 tttccagaga aagtgcagag ctaaaggagt tagatggtga ggcagcaggt ggtcggcagg
     3361 cattctggta cccagttgta gccaggcttt ggttatcctc tcccagctgc atgtctcatg
     3421 tctcttagat ttgcatacag cctcatacag tgcaaaggaa gatgtgatct ctaaacaaaa
     3481 aggaataaaa acctaaaatg atgatattct actcagggta aaacaaacaa acaaacccct
     3541 tccaccaaaa agcctgaagc ttgcagtcag tttacctcat ttggaatgtt ttgttgagtt
     3601 gtgttacagg aatttttttt tattagtgta taaaattata cccatctcct tgcctttggc
     3661 tcctggttat tgcctcttca aacataagta caatctatag gagaatgggg gtgtggctgt
     3721 ccttcggctg tttactgtag ccatcagtgc ttggcaaggt cagaaggtag ctgggtctgg
     3781 gactggagga ggcgtctgtc tccagcaaag agcagggtct ggtgccccaa caggacaacc
     3841 agagagctgt gtaggttgcc ctcctgcagt ctggaccttt cctcagagcc agctaggatg
     3901 gtgacccttg tctttaggca aggtatttag ggacaattgg ccaagagggt catggcagca
     3961 ctattgagaa atggcacagg tacaagcagc tgatctccca ttccaggatt ggaccaggag
     4021 tatgaatttc tgatgttcta cacccccaca ccccacacac acccaaaaaa ggttgatgga
     4081 tttgggcaac catagacctc agcaggaatc tgcacggtgt gaggagcccc ctgttggcgc
     4141 cgtggttagt ccttctggga accagctgct tgggtgtaga ttgtcctcag ctgtctctag
     4201 gacttgcact cacaggaagc tttgcactcg ggccctcact cgtttctctt ctctgaggag
     4261 agcagtgaat gggaccccca aagtgagctt gtaacaaggg gtagtaatgg ccctcccact
     4321 cactctgcct atgacaccct gggagctgac ataggaaaca ggagcctagg gaggatgtat
     4381 ggaaggtttc aatggtttaa atatggcttc ttggtttgct agggctgtgc tcttaatggt
     4441 ccattcctag gttattggct tttcaccctc cacagttggc atggtgaaga aacatttgag
     4501 cagtgttgag gctgaagtgt tgctaggtga aggagctaag tgattttaga acacaagaga
     4561 ctctcagaac ctaagaggtt ttggaaagtg aggactctgc ttgcagttgg tggttgtgga
     4621 gagccaatgt gtgaaacagg gtagacagac gcttcggcca tgcttgtacc agtggtcctt
     4681 cccccatctt tccacaagcc cagcctctaa tgctaaggac cttggagaag agggaacctc
     4741 ctggctaaac tagaaccacg aagacccttc atggagtggt ttcgccaggg aatgacctta
     4801 gggtaaggcc aagaagagtg aattctggca agtcgattaa gcctttcctt ccacgacagc
     4861 catcaccact gtcaccctgt agacgagact tgggttacca ctagcatctc cagtttagcc
     4921 tcttccaaga gactagttgg cagcagggct gatggaccta ggaggtggtc aggtgatctt
     4981 ggtcccaccc aattgcctta taaatgtggt ccttcccagg caggagggta aggctgagag
     5041 aagagagctc agtgcccttg ggcagcacta gtttggccac aggagtactg tcctctgttg
     5101 actttggttc ataagatgat ggtacaatgc caaggagagg cttggggtgt ggagagccct
     5161 ctaggtagaa ggcaatggga ctccccttcc ctttcagagt cagtggatgg gaaaaggttg
     5221 ttttccggat caaggcagtg ggctgatggg gtaatggagg tgcctgagtt ttgcctgagg
     5281 ctttgtatta tgctgaatgt gtccagaggg acaaatttgc agaacctcat attgatattt
     5341 taaataataa aataaaaagc actttaggtt acttttatct ttaacccaat tgctgcaatt
     5401 tctgttgtgt atgtatatat acatatatat actttcccca aagttttatt ttttgctcag
     5461 aataaaagtt aagttgaggt gtaaaaagag cacttacttg ggtgcaatat atgcgtagct
     5521 tgacagtcgc tatcccacgt ggccctggcc tggccttctg ctccatcagc cctgtgctga
     5581 agctggccac agggaaccac tatcagcatc tcagcagctg ctcaatctat gcaagccttc
     5641 ctgtgtgtcc ggggcgcccc cctcaggccc tccaaggaac tgctgcagct gttttcttct
     5701 gactgttgag gccccttttc aactgcttct ccgccgtccc ctgctctttc ccctcttccc
     5761 cagaacaaaa tgattcctga aaggaagggt cggttgttcc taggtagaaa cctggcacct
     5821 ttagactttc atatttgtaa acacatccat tgaagggaag cgtctccagt gtcactgaga
     5881 ctgttggtgt gtggaagtgc actactgtcg cgaatttaca gctttttcac agccccaatc
     5941 agaaatatgt cggaaggctc tctgcaccac ctctgttggc tcaaaagcag aatgacttcc
     6001 acgcttcatc aaatccattt aggaagccgt taatgacctc cctcaccggc tcccacttac
     6061 tcttaaattc ccaactgtag aggcatttta aatatactcc agccagtgca agattttcac
     6121 agatgaagtt tgcaagtggt tgtgctgtga cacagatggg aaattgtcca tctctcctct
     6181 gaggcagaag gggatgagcc ataaaactgg atcatacata tttctgattg gtctgtttct
     6241 tgcatattct tggatcttgt ttcttatgcc cctgctcttg gggaggttgg tcattagccc
     6301 aggcatctct cactgttctg gcggcttgag ggcttctcag tctgcgttga aaatgcaagt
     6361 ttaattggat ctgtcaaatt atattatagt acaacctttt tctagaactg gcgtgtaaaa
     6421 accatacact gctccttggt ggtggctcac tgggacaaaa ataggtgata atgtcatgtc
     6481 actttctcaa ttcaatgcaa tttctacttt tttttttttt aaacaggtaa tgttttcata
     6541 aaactttttt ccaccaaaat ctggggtgtt tattgtagta tccccgtgtc cctcgaggtg
     6601 ccaagtcagg ggcttgcccg cccttgccgc gggtgttctc aggcttccca agggctgtgt
     6661 gagtcaacag cctgcatctt tggttgtgct ttggagaaaa tgtcactgag gttggagtcc
     6721 caggtagcta gtcccttttc ctgggaattc cctgttgcag tttggggaag gtggctggaa
     6781 aacgggcttt tctggttgcc tggctcagat ctagccagaa aaggccacaa actcttttct
     6841 aacacaaact aatcattggc cagtggtctt ggtgacaagt ttttaagtcc caaatagttt
     6901 tatttgaatt atgtaaaagt accaagttta ttttaaatgt aaaacatggg aacaacggac
     6961 ttccactgag cgatatgaaa acgttacagg ttcagtactt ccaaaggaag aaacctccaa
     7021 cccctaaaaa gaataaatac gaatttgtat ttttgaagaa tgtgaaataa tagtgtttgc
     7081 ttaattgctc attttgtata aacttaatat tgtactttaa aatatctgct atgaagtgaa
     7141 aatttaactt tttggaattg aaaaagcaat attaaatact aatgaaatct taattaaatg
     7201 cttatttaaa tctggtagta catgtggcat tacttcccat ccctgtccct ggttgacact
     7261 ctcttcccac tcccagccat caagtcttgg agggacagaa aagaaaggtc agtcaccagg
     7321 gtctgcagat ttccttttaa tcaagactct gctcaagtgt tttgtggggc tgagagcccc
     7381 caaagcatga aatgaacatg taataccacc tggaaccccc aaagcaggcc agaccactct
     7441 ggccagcact gctggcttcc tgaatccgag tactcaagac tggatgtttg ttggctccat
     7501 ttcaaagcac agtactgcct tcagccagga cgacgtggga gtgaacccag ctgctagtag
     7561 agttgccatc ccaggctgag ggccaagtac cagcaactgc ccgtgaagac tggccccttt
     7621 tagtgaaggg a
//
