LOCUS       NP_034964                376 aa            linear   ROD 24-SEP-2024
DEFINITION  growth/differentiation factor 8 preproprotein [Mus musculus].
ACCESSION   NP_034964
VERSION     NP_034964.1
DBSOURCE    REFSEQ: accession NM_010834.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 376)
  AUTHORS   Wang,H., Guo,S., Gao,H., Ding,J., Li,H., Kong,X., Zhang,S., He,M.,
            Feng,Y., Wu,W., Xu,K., Chen,Y., Zhang,H., Liu,T. and Kong,X.
  TITLE     Myostatin regulates energy homeostasis through autocrine- and
            paracrine-mediated microenvironment communication
  JOURNAL   J Clin Invest 134 (16), e178303 (2024)
   PUBMED   38889010
  REMARK    GeneRIF: Myostatin regulates energy homeostasis through autocrine-
            and paracrine-mediated microenvironment communication.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 376)
  AUTHORS   Morel,J., Pignard,A.S., Castells,J., Allibert,V., Hatimi,L.,
            Buhot,B., Velarde,M., Durieux,A.C. and Freyssenet,D.
  TITLE     Myostatin gene invalidation does not prevent skeletal muscle mass
            loss during experimental sepsis in mice
  JOURNAL   J Physiol 602 (12), 2839-2854 (2024)
   PUBMED   38748517
  REMARK    GeneRIF: Myostatin gene invalidation does not prevent skeletal
            muscle mass loss during experimental sepsis in mice.
REFERENCE   3  (residues 1 to 376)
  AUTHORS   Martin Gil,C., Raoof,R., Versteeg,S., Willemen,H.L.D.M.,
            Lafeber,F.P.J.G., Mastbergen,S.C. and Eijkelkamp,N.
  TITLE     Myostatin and CXCL11 promote nervous tissue macrophages to maintain
            osteoarthritis pain
  JOURNAL   Brain Behav Immun 116, 203-215 (2024)
   PUBMED   38070625
  REMARK    GeneRIF: Myostatin and CXCL11 promote nervous tissue macrophages to
            maintain osteoarthritis pain.
REFERENCE   4  (residues 1 to 376)
  AUTHORS   Riasi,M., Mozaffari-Jovin,S. and Javadmanesh,A.
  TITLE     The effect of modification of DNA interference on myostatin gene
            expression in mice
  JOURNAL   J Genet 103 (2024)
   PUBMED   38185835
  REMARK    GeneRIF: The effect of modification of DNA interference on
            myostatin gene expression in mice.
REFERENCE   5  (residues 1 to 376)
  AUTHORS   Knapp,M., Supruniuk,E. and Gorski,J.
  TITLE     Myostatin and the Heart
  JOURNAL   Biomolecules 13 (12), 1777 (2023)
   PUBMED   38136649
  REMARK    GeneRIF: Myostatin and the Heart.
            Review article
            Publication Status: Online-Only
REFERENCE   6  (residues 1 to 376)
  AUTHORS   Conlon,I. and Raff,M.
  TITLE     Size control in animal development
  JOURNAL   Cell 96 (2), 235-244 (1999)
   PUBMED   9988218
  REMARK    Review article
REFERENCE   7  (residues 1 to 376)
  AUTHORS   Szabo,G., Dallmann,G., Muller,G., Patthy,L., Soller,M. and Varga,L.
  TITLE     A deletion in the myostatin gene causes the compact (Cmpt)
            hypermuscular mutation in mice
  JOURNAL   Mamm Genome 9 (8), 671-672 (1998)
   PUBMED   9680391
REFERENCE   8  (residues 1 to 376)
  AUTHORS   McPherron,A.C. and Lee,S.J.
  TITLE     Double muscling in cattle due to mutations in the myostatin gene
  JOURNAL   Proc Natl Acad Sci U S A 94 (23), 12457-12461 (1997)
   PUBMED   9356471
REFERENCE   9  (residues 1 to 376)
  AUTHORS   Varga,L., Szabo,G., Darvasi,A., Muller,G., Sass,M. and Soller,M.
  TITLE     Inheritance and mapping of Compact (Cmpt), a new mutation causing
            hypermuscularity in mice
  JOURNAL   Genetics 147 (2), 755-764 (1997)
   PUBMED   9335610
REFERENCE   10 (residues 1 to 376)
  AUTHORS   McPherron,A.C., Lawler,A.M. and Lee,S.J.
  TITLE     Regulation of skeletal muscle mass in mice by a new TGF-beta
            superfamily member
  JOURNAL   Nature 387 (6628), 83-90 (1997)
   PUBMED   9139826
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK142222.1 and AK132415.1.
            
            Summary: This gene encodes a secreted ligand of the TGF-beta
            (transforming growth factor-beta) superfamily of proteins. Ligands
            of this family bind various TGF-beta receptors leading to
            recruitment and activation of SMAD family transcription factors
            that regulate gene expression. The encoded preproprotein is
            proteolytically processed to generate each subunit of the
            disulfide-linked homodimer. This protein negatively regulates
            skeletal muscle cell proliferation and differentiation. Homozygous
            knockout mice for this gene exhibit increased muscle mass and bone
            density, and reduced adiposity. [provided by RefSeq, Jul 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK142222.1, AK132415.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 26.99 cM"
     Protein         1..376
                     /product="growth/differentiation factor 8 preproprotein"
                     /note="growth/differentiation factor 8"
                     /calculated_mol_wt=40087
     sig_peptide     1..24
                     /note="/evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O08689.1)"
                     /calculated_mol_wt=2853
     Region          37..254
                     /region_name="TGFb_propeptide"
                     /note="TGF-beta propeptide; cl46657"
                     /db_xref="CDD:480997"
     Site            72
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (O08689.1)"
     Site            99..100
                     /site_type="cleavage"
                     /note="Cleavage. /evidence=ECO:0000269|PubMed:14671324;
                     propagated from UniProtKB/Swiss-Prot (O08689.1)"
     mat_peptide     268..376
                     /product="Growth/differentiation factor 8.
                     /id=PRO_0000033957"
                     /note="propagated from UniProtKB/Swiss-Prot (O08689.1)"
                     /calculated_mol_wt=12407
     Region          269..376
                     /region_name="TGF_beta_GDF8"
                     /note="transforming growth factor beta (TGF-beta) like
                     domain found in growth/differentiation factor 8 (GDF8) and
                     similar proteins; cd19388"
                     /db_xref="CDD:381658"
     Site            order(269..271,279,281,287,294..298,300,303,305..309,311,
                     316..319,322..324,326..328,330..331,333..335,340,342,346,
                     350..362,365..368,370,376)
                     /site_type="other"
                     /note="polypeptide substrate binding site 2 [polypeptide
                     binding]"
                     /db_xref="CDD:381658"
     Site            order(287,289,291,294,296,298,305,307..311,319..320,
                     322..324,326..328,331..332,334..338,340..343,346,351,353,
                     365..368,370,376)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:381658"
     Site            order(292,297..298,300..303,350,352,354,358..362)
                     /site_type="other"
                     /note="polypeptide substrate binding site 1 [polypeptide
                     binding]"
                     /db_xref="CDD:381658"
     CDS             1..376
                     /gene="Mstn"
                     /gene_synonym="Cmpt; Gdf8"
                     /coded_by="NM_010834.3:127..1257"
                     /db_xref="CCDS:CCDS14950.1"
                     /db_xref="GeneID:17700"
                     /db_xref="MGI:MGI:95691"
ORIGIN      
        1 mmqklqmyvy iylfmliaag pvdlnegser eenvekeglc nacawrqntr ysrieaikiq
       61 ilsklrleta pniskdairq llprapplre lidqydvqrd dssdgsledd dyhattetii
      121 tmptesdflm qadgkpkccf fkfsskiqyn kvvkaqlwiy lrpvktpttv fvqilrlikp
      181 mkdgtrytgi rslkldmspg tgiwqsidvk tvlqnwlkqp esnlgieika ldenghdlav
      241 tfpgpgedgl npflevkvtd tpkrsrrdfg ldcdehstes rccrypltvd feafgwdwii
      301 apkrykanyc sgecefvflq kyphthlvhq anprgsagpc ctptkmspin mlyfngkeqi
      361 iygkipamvv drcgcs
//
