LOCUS       NM_001201341             780 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus musashi RNA-binding protein 2 (Msi2), transcript
            variant 2, mRNA.
ACCESSION   NM_001201341 XM_003085515
VERSION     NM_001201341.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Banik,J., Moreira,A.R.S., Lim,J., Tomlinson,S., Hardy,L.L.,
            Lagasse,A., Haney,A., Crimmins,M.R., Boehm,U., Odle,A.K.,
            MacNicol,M.C., Childs,G.V. and MacNicol,A.M.
  TITLE     The Musashi RNA binding proteins direct the translational
            activation of key pituitary mRNAs
  JOURNAL   Sci Rep 14 (1), 5918 (2024)
   PUBMED   38467682
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 780)
  AUTHORS   Wang,R., Kato,F., Watson,R.Y., Beedle,A.M., Call,J.A., Tsunoda,Y.,
            Noda,T., Tsuchiya,T., Kashima,M., Hattori,A. and Ito,T.
  TITLE     The RNA-binding protein Msi2 regulates autophagy during myogenic
            differentiation
  JOURNAL   Life Sci Alliance 7 (5), e202302016 (2024)
   PUBMED   38373797
  REMARK    GeneRIF: The RNA-binding protein Msi2 regulates autophagy during
            myogenic differentiation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 780)
  AUTHORS   Rajbhandari,N., Hamilton,M., Quintero,C.M., Ferguson,L.P., Fox,R.,
            Schurch,C.M., Wang,J., Nakamura,M., Lytle,N.K., McDermott,M.,
            Diaz,E., Pettit,H., Kritzik,M., Han,H., Cridebring,D., Wen,K.W.,
            Tsai,S., Goggins,M.G., Lowy,A.M., Wechsler-Reya,R.J., Von
            Hoff,D.D., Newman,A.M. and Reya,T.
  TITLE     Single-cell mapping identifies MSI+ cells as a common origin for
            diverse subtypes of pancreatic cancer
  JOURNAL   Cancer Cell 41 (11), 1989-2005 (2023)
   PUBMED   37802055
REFERENCE   5  (bases 1 to 780)
  AUTHORS   Singh,S., Gaur,A., Sharma,R.K., Kumari,R., Prakash,S., Kumari,S.,
            Chaudhary,A.D., Prasun,P., Pant,P., Hunkler,H., Thum,T.,
            Jagavelu,K., Bharati,P., Hanif,K., Chitkara,P., Kumar,S., Mitra,K.
            and Gupta,S.K.
  TITLE     Musashi-2 causes cardiac hypertrophy and heart failure by inducing
            mitochondrial dysfunction through destabilizing Cluh and Smyd1 mRNA
  JOURNAL   Basic Res Cardiol 118 (1), 46 (2023)
   PUBMED   37923788
  REMARK    GeneRIF: Musashi-2 causes cardiac hypertrophy and heart failure by
            inducing mitochondrial dysfunction through destabilizing Cluh and
            Smyd1 mRNA.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 780)
  AUTHORS   Aubert,J., Stavridis,M.P., Tweedie,S., O'Reilly,M., Vierlinger,K.,
            Li,M., Ghazal,P., Pratt,T., Mason,J.O., Roy,D. and Smith,A.
  TITLE     Screening for mammalian neural genes via fluorescence-activated
            cell sorter purification of neural precursors from Sox1-gfp
            knock-in mice
  JOURNAL   Proc Natl Acad Sci U S A 100 Suppl 1 (Suppl 1), 11836-11841 (2003)
   PUBMED   12923295
REFERENCE   7  (bases 1 to 780)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   8  (bases 1 to 780)
  AUTHORS   Sakakibara,S., Nakamura,Y., Yoshida,T., Shibata,S., Koike,M.,
            Takano,H., Ueda,S., Uchiyama,Y., Noda,T. and Okano,H.
  TITLE     RNA-binding protein Musashi family: roles for CNS stem cells and a
            subpopulation of ependymal cells revealed by targeted disruption
            and antisense ablation
  JOURNAL   Proc Natl Acad Sci U S A 99 (23), 15194-15199 (2002)
   PUBMED   12407178
  REMARK    GeneRIF: data suggest that msi1 and msi2 are cooperatively involved
            in the proliferation and maintenance of CNS stem cell populations
REFERENCE   9  (bases 1 to 780)
  AUTHORS   Sakakibara,S., Nakamura,Y., Satoh,H. and Okano,H.
  TITLE     Rna-binding protein Musashi2: developmentally regulated expression
            in neural precursor cells and subpopulations of neurons in
            mammalian CNS
  JOURNAL   J Neurosci 21 (20), 8091-8107 (2001)
   PUBMED   11588182
REFERENCE   10 (bases 1 to 780)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL645601.13.
            
            On Jan 15, 2011 this sequence version replaced XM_003085515.1.
            
            Transcript Variant: This variant (2) represents an alternate splice
            pattern in the 3' coding region and UTR, compared to variant 1. The
            resulting isoform (2) has a substantially shorter and distinct
            C-terminus, compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from genomic sequence
            data to make the sequence consistent with the reference genome
            assembly. The genomic coordinates used for the transcript record
            were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC109368.1, BI452804.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-239               AL645601.13        117086-117324       c
            240-280             AL645601.13        116762-116802       c
            281-362             AL645601.13        116347-116428       c
            363-447             AL645601.13        115567-115651       c
            448-489             AL645601.13        111495-111536       c
            490-561             AL645601.13        86934-87005         c
            562-658             AL645601.13        86509-86605         c
            659-780             AL645601.13        85011-85132         c
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 52.4 cM"
     gene            1..780
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /note="musashi RNA-binding protein 2"
                     /db_xref="GeneID:76626"
                     /db_xref="MGI:MGI:1923876"
     exon            1..239
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /inference="alignment:Splign:2.1.0"
     CDS             178..654
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /note="isoform 2 is encoded by transcript variant 2;
                     RNA-binding protein Musashi homolog 2; Musashi2;
                     musashi-2"
                     /codon_start=1
                     /product="RNA-binding protein Musashi homolog 2 isoform 2"
                     /protein_id="NP_001188270.1"
                     /db_xref="CCDS:CCDS48882.1"
                     /db_xref="GeneID:76626"
                     /db_xref="MGI:MGI:1923876"
                     /translation="MEANGSPGTSGSANDSQHDPGKMFIGGLSWQTSPDSLRDYFSKF
                     GEIRECMVMRDPTTKRSRGFGFVTFADPASVDKVLGQPHHELDSKTIDPKVAFPRRAQ
                     PKSQEQGGSWKRTWTTVQARAASGDQKKVGLSDSAALFPALPVPLRPGRRGFRGAV"
     exon            240..280
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /inference="alignment:Splign:2.1.0"
     exon            281..362
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /inference="alignment:Splign:2.1.0"
     exon            363..447
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /inference="alignment:Splign:2.1.0"
     exon            448..489
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /inference="alignment:Splign:2.1.0"
     exon            490..561
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /inference="alignment:Splign:2.1.0"
     exon            562..658
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /inference="alignment:Splign:2.1.0"
     exon            659..780
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /inference="alignment:Splign:2.1.0"
     regulatory      757..762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /note="hexamer: AATAAA"
     polyA_site      780
                     /gene="Msi2"
                     /gene_synonym="1700105C15Rik; Msi2h"
                     /note="major polyA site"
ORIGIN      
        1 gtgacgggct ctcgccgctg gcccggctcg gcgctcgcgg agcttcgcag ccgccgggcg
       61 gggggaggag gcgggggagg agggacggag atctggggct cggagccggc cgccgctgcg
      121 ctccgcttcg ctgtggggct cggtgtgtcg ggggtggggg ggcggggggg ctccgctatg
      181 gaggcaaatg ggagcccagg cacctcgggc agcgccaacg actcccagca cgaccccggt
      241 aaaatgttta tcggtggact gagctggcag acctcaccag atagccttag agactatttt
      301 agcaaatttg gagaaattag agaatgtatg gtcatgagag atcccacaac gaaacgctcc
      361 agaggcttcg gtttcgtcac cttcgcagac ccagcaagtg tagataaagt attaggtcag
      421 ccccaccatg agttagattc caagacgatt gacccaaaag ttgcatttcc tcgtcgagcg
      481 caacctaaga gtcaagagca gggaggctcc tggaaacgca catggaccac ggtccaagct
      541 cgggctgcct caggtgacca gaaaaaggtt ggcttgtcag actctgctgc cctcttcccg
      601 gctctccccg tccctctgcg gcctggcaga cgaggcttta gaggggccgt ctgatgggca
      661 acccggagct ttctgcaatc cagctctggc ttttcttacc ccagccgttc tttctcacag
      721 cgaagaggat acaattcagc accaataatg ccgagcaata aataacccag cgaagatgaa
//
