LOCUS       NM_001146215            2697 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus mutS homolog 5 (Msh5), transcript variant 2, mRNA.
ACCESSION   NM_001146215
VERSION     NM_001146215.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Chen,M., Yao,C., Qin,Y., Cui,X., Li,P., Ji,Z., Lin,L., Wu,H.,
            Zhou,Z., Gui,Y., Li,Z. and Gao,F.
  TITLE     Mutations of MSH5 in nonobstructive azoospermia (NOA) and rescued
            via in vivo gene editing
  JOURNAL   Signal Transduct Target Ther 7 (1), 1 (2022)
   PUBMED   34980881
  REMARK    GeneRIF: Mutations of MSH5 in nonobstructive azoospermia (NOA) and
            rescued via in vivo gene editing.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2697)
  AUTHORS   ElInati,E., Zielinska,A.P., McCarthy,A., Kubikova,N., Maciulyte,V.,
            Mahadevaiah,S., Sangrithi,M.N., Ojarikre,O., Wells,D., Niakan,K.K.,
            Schuh,M. and Turner,J.M.A.
  TITLE     The BCL-2 pathway preserves mammalian genome integrity by
            eliminating recombination-defective oocytes
  JOURNAL   Nat Commun 11 (1), 2598 (2020)
   PUBMED   32451402
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2697)
  AUTHORS   Shi,B., Xue,J., Zhou,J., Kasowitz,S.D., Zhang,Y., Liang,G.,
            Guan,Y., Shi,Q., Liu,M., Sha,J., Huang,X. and Wang,P.J.
  TITLE     MORC2B is essential for meiotic progression and fertility
  JOURNAL   PLoS Genet 14 (1), e1007175 (2018)
   PUBMED   29329290
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2697)
  AUTHORS   Shin,Y.H., Ren,Y., Suzuki,H., Golnoski,K.J., Ahn,H.W., Mico,V. and
            Rajkovic,A.
  TITLE     Transcription factors SOHLH1 and SOHLH2 coordinate oocyte
            differentiation without affecting meiosis I
  JOURNAL   J Clin Invest 127 (6), 2106-2117 (2017)
   PUBMED   28504655
REFERENCE   6  (bases 1 to 2697)
  AUTHORS   Allcock,R.J., Martin,A.M. and Price,P.
  TITLE     The mouse as a model for the effects of MHC genes on human disease
  JOURNAL   Immunol Today 21 (7), 328-332 (2000)
   PUBMED   10871872
  REMARK    Review article
REFERENCE   7  (bases 1 to 2697)
  AUTHORS   Kneitz,B., Cohen,P.E., Avdievich,E., Zhu,L., Kane,M.F., Hou,H. Jr.,
            Kolodner,R.D., Kucherlapati,R., Pollard,J.W. and Edelmann,W.
  TITLE     MutS homolog 4 localization to meiotic chromosomes is required for
            chromosome pairing during meiosis in male and female mice
  JOURNAL   Genes Dev 14 (9), 1085-1097 (2000)
   PUBMED   10809667
REFERENCE   8  (bases 1 to 2697)
  AUTHORS   Her,C., Wu,X., Wan,W. and Doggett,N.A.
  TITLE     Identification and characterization of the mouse MutS homolog 5:
            Msh5
  JOURNAL   Mamm Genome 10 (11), 1054-1061 (1999)
   PUBMED   10556423
REFERENCE   9  (bases 1 to 2697)
  AUTHORS   de Vries,S.S., Baart,E.B., Dekker,M., Siezen,A., de Rooij,D.G., de
            Boer,P. and te Riele,H.
  TITLE     Mouse MutS-like protein Msh5 is required for proper chromosome
            synapsis in male and female meiosis
  JOURNAL   Genes Dev 13 (5), 523-531 (1999)
   PUBMED   10072381
REFERENCE   10 (bases 1 to 2697)
  AUTHORS   Edelmann,W., Cohen,P.E., Kneitz,B., Winand,N., Lia,M., Heyer,J.,
            Kolodner,R., Pollard,J.W. and Kucherlapati,R.
  TITLE     Mammalian MutS homologue 5 is required for chromosome pairing in
            meiosis
  JOURNAL   Nat Genet 21 (1), 123-127 (1999)
   PUBMED   9916805
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC087117.9.
            
            On Jan 13, 2015 this sequence version replaced NM_001146215.1.
            
            Summary: This gene encodes a member of the MutS family of proteins
            that play critical roles in DNA mismatch repair and meiotic
            homologous recombination processes. Mice lacking the encoded
            protein are viable but sterile, with severe defects in
            spermatogenesis in males and complete loss of ovarian structures in
            females. Mutations in a similar gene in humans have been shown to
            cause common variable immune deficiency (CVID) and immunoglobulin A
            deficiency. Alternative splicing of this gene results in multiple
            transcript variants. [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1 and 2 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF107352.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-46                AC087117.9         155317-155362       c
            47-203              AC087117.9         154848-155004       c
            204-327             AC087117.9         154547-154670       c
            328-408             AC087117.9         153911-153991       c
            409-471             AC087117.9         153731-153793       c
            472-593             AC087117.9         152934-153055       c
            594-703             AC087117.9         152642-152751       c
            704-739             AC087117.9         152346-152381       c
            740-822             AC087117.9         150715-150797       c
            823-868             AC087117.9         150087-150132       c
            869-1007            AC087117.9         147818-147956       c
            1008-1070           AC087117.9         147023-147085       c
            1071-1199           AC087117.9         142207-142335       c
            1200-1272           AC087117.9         141840-141912       c
            1273-1382           AC087117.9         141377-141486       c
            1383-1463           AC087117.9         140958-141038       c
            1464-1551           AC087117.9         140296-140383       c
            1552-1741           AC087117.9         139971-140160       c
            1742-1868           AC087117.9         139765-139891       c
            1869-2018           AC087117.9         139226-139375       c
            2019-2093           AC087117.9         138818-138892       c
            2094-2237           AC087117.9         138495-138638       c
            2238-2375           AC087117.9         138270-138407       c
            2376-2449           AC087117.9         137813-137886       c
            2450-2697           AC087117.9         137222-137469       c
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 18.57 cM"
     gene            1..2697
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /note="mutS homolog 5"
                     /db_xref="GeneID:17687"
                     /db_xref="MGI:MGI:1329021"
     exon            1..46
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            47..203
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..2561
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /note="DNA mismatch repair protein Msh5"
                     /codon_start=1
                     /product="mutS protein homolog 5"
                     /protein_id="NP_001139687.1"
                     /db_xref="CCDS:CCDS28674.1"
                     /db_xref="GeneID:17687"
                     /db_xref="MGI:MGI:1329021"
                     /translation="MAFRATPGRTPPGPGPRSGIPSASFPSPQPPMAGPGGIEEEDEE
                     EPAEIHLCVLWSSGYLGIAYYDTSDSTIHFMPDAPDHESLKLLQRVLDEINPQSVVTS
                     AKQDEAMTRFLGKLASEEHREPKGPEIILLPSVDFGPEISKQRLLSGNYSFISDSMTA
                     TEKILFLSSIIPFDCVLTVRALGGLLKFLSRRRIGVELEDYDVGVPILGFKKFVLTHL
                     VSIDQDTYSVLQIFKSESHPSVYKVASGLKEGLSLFGILNRCRCKWGQKLLRLWFTRP
                     TRELRELNSRLDVIQFFLMPQNLDMAQMLHRLLSHIKNVPLILKRMKLSHTKVSDWQV
                     LYKTVYSALGLRDACRSLPQSIQLFQDIAQEFSDDLHHIASLIGKVVDFEESLAENRF
                     TVLPNIDPDIDAKKRRLIGLPSFLTEVAQKELENLDSRIPSCSVIYIPLIGFLLSIPR
                     LPFMVEASDFEIEGLDFMFLSEDKLHYRSARTKELDTLLGDLHCEIRDQETLLMYQLQ
                     CQVLARASVLTRVLDLASRLDVLLALASAARDYGYSRPHYSPCIHGVRIRNGRHPLME
                     LCARTFVPNSTDCGGDQGRVKVITGPNSSGKSIYLKQVGLITFMALVGSFVPAEEAEI
                     GVIDAIFTRIHSCESISLGLSTFMIDLNQVAKAVNNATEHSLVLIDEFGKGTNSVDGL
                     ALLAAVLRHWLALGPSCPHVFVATNFLSLVQLQLLPQGPLVQYLTMETCEDGEDLVFF
                     YQLCQGVASASHASHTAAQAGLPDPLIARGKEVSDLIRSGKPIKATNELLRRNQMENC
                     QALVDTFLKLDLEDPTLDLDIFISQEVLPAAPTIL"
     exon            204..327
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            328..408
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            409..471
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            472..593
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            594..703
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            704..739
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            740..822
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            823..868
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            869..1007
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            1008..1070
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            1071..1199
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            1200..1272
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1382
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1463
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            1464..1551
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            1552..1741
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..1868
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            1869..2018
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            2019..2093
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2237
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..2375
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            2376..2449
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     exon            2450..2697
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2676..2681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /note="hexamer: AATAAA"
     polyA_site      2697
                     /gene="Msh5"
                     /gene_synonym="Mut5"
                     /note="major polyA site"
ORIGIN      
        1 gcaggcttgc ggcggttggt cagggaggtg gatcgtcgcg gctgagagtc gccgagccca
       61 tggctttcag agcgacccca ggccggacgc cgccgggacc cggacccaga tccggaatcc
      121 cctcagccag cttccccagc cctcagcccc caatggcggg gcctggaggt atcgaggaag
      181 aggacgagga ggagcccgcc gagatccatc tgtgcgtgct gtggagctcg ggatacctgg
      241 gcattgctta ctatgacact agtgactcca ctatccactt catgccagat gccccagacc
      301 acgagagcct aaagcttctc cagagagttc tggatgaaat caacccccag tctgttgtca
      361 caagtgccaa acaggatgag gctatgactc gatttctagg gaagcttgcc tccgaggagc
      421 acagagagcc aaagggacct gaaatcatac ttctgccaag cgtggatttt ggtccagaga
      481 taagcaaaca gcgtctcctt tccggaaact actccttcat ctcagactcc atgactgcta
      541 ctgagaaaat ccttttcctc tcctccatta ttccctttga ctgtgtcctc acggtccggg
      601 cacttggagg actgctcaag ttcctgagtc gaagaagaat tggggttgaa ctggaagact
      661 atgatgttgg cgtccctatc ctgggattca agaagtttgt attgacccat ctggtgagca
      721 tagatcaaga cacttacagc gttctacaga ttttcaagag tgagtctcac ccctcggtgt
      781 acaaagtagc cagtgggctg aaggaggggc tcagcctttt tggaatcctc aacagatgcc
      841 gctgtaagtg gggacagaag ctgctcaggc tgtggtttac acgtccaacc cgggagctaa
      901 gggaactcaa ttcccgactg gatgtcattc agttcttcct gatgcctcag aacctggaca
      961 tggcccagat gctgcaccga ctcctgagcc acatcaagaa tgtgcctctg attctgaaac
     1021 gcatgaagtt gtcccacacc aaggtcagtg actggcaggt cctctacaag actgtgtaca
     1081 gtgctctcgg cctgagggat gcctgccgtt ctctgccaca gtccatccag ctttttcagg
     1141 acattgccca ggagttctct gacgacctgc atcacattgc cagcctcatc gggaaggtgg
     1201 tggactttga ggaaagtctt gctgaaaatc gcttcacagt cctccctaac atagaccctg
     1261 acatagatgc caagaagcga aggctgatag ggcttccgag cttcctcact gaagttgctc
     1321 agaaggagct ggagaacctg gactctcgca tcccctcatg cagtgtcatc tacatccctc
     1381 tgattggctt ccttctttcc attccccgct tgcctttcat ggtggaagct agtgactttg
     1441 agattgaggg gctggacttc atgtttctct cagaggacaa gctgcactat cgtagcgccc
     1501 ggaccaagga gctggacacg ctgctgggag acctgcactg cgagatccgg gaccaggaga
     1561 ccctgttgat gtaccagctg cagtgccagg tgctggcacg ggcttcggtc ttgactcggg
     1621 tattggacct tgcctcccgc ctggacgtcc tgttggctct tgccagtgct gcccgggact
     1681 acggctattc gagaccgcat tactctccct gtatccatgg agtacgaatc aggaatggca
     1741 ggcatcctct gatggaactg tgtgcacgaa ccttcgtgcc caactccacg gactgtggtg
     1801 gggaccaggg cagggtcaaa gtcatcactg ggcccaactc ctcagggaaa agcatatatc
     1861 tcaagcaggt aggcttgatc actttcatgg ccctggtggg cagtttcgtg cctgcagagg
     1921 aggccgagat tggggtaatc gacgccatct tcactcgaat tcacagctgc gaatccatct
     1981 ccctcggcct ctccaccttc atgattgatc tcaaccaggt ggcgaaagca gtgaacaatg
     2041 ccacagagca ctcgctggtc ctgatcgatg aattcgggaa ggggaccaac tcggtggatg
     2101 gcctggcact tctggctgct gtgctccgtc actggctagc actgggaccc agctgccccc
     2161 acgtctttgt agccaccaac ttcctgagcc ttgttcagct gcagctgctg ccgcaaggac
     2221 ccctggtgca gtatttgacc atggagactt gtgaggatgg ggaagacctt gtcttcttct
     2281 accagctttg ccaaggcgtc gccagtgcca gccacgcctc ccacacagcg gcccaggctg
     2341 ggcttcctga cccactcatt gctcgtggca aagaggtctc agacttgatc cgcagtggga
     2401 aacccatcaa ggccacgaat gagcttctga ggaggaacca aatggaaaac tgccaggcac
     2461 tggtggatac gtttctaaaa ctggacttgg aggatcccac cctggacctg gacattttca
     2521 ttagtcagga agtgctgccc gctgctccca ccatcctctg agagtcctcc ctcgcctccc
     2581 agctacctga gccccggggc ctaccatgcc ttttcgggtt tttttttttc ccttactgca
     2641 gagttctcag tttgcacatt gtgtcttgtg ctaggaataa agcttatttt agatcaa
//
