LOCUS       NM_025369                716 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus mitochondrial ribosomal protein S36 (Mrps36),
            transcript variant 1, mRNA.
ACCESSION   NM_025369
VERSION     NM_025369.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 716)
  AUTHORS   Hevler,J.F., Albanese,P., Cabrera-Orefice,A., Potter,A.,
            Jankevics,A., Misic,J., Scheltema,R.A., Brandt,U., Arnold,S. and
            Heck,A.J.R.
  TITLE     MRPS36 provides a structural link in the eukaryotic 2-oxoglutarate
            dehydrogenase complex
  JOURNAL   Open Biol 13 (3), 220363 (2023)
   PUBMED   36854377
REFERENCE   3  (bases 1 to 716)
  AUTHORS   Cheong,A., Lingutla,R. and Mager,J.
  TITLE     Expression analysis of mammalian mitochondrial ribosomal protein
            genes
  JOURNAL   Gene Expr Patterns 38, 119147 (2020)
   PUBMED   32987154
REFERENCE   4  (bases 1 to 716)
  AUTHORS   Heublein,M., Burguillos,M.A., Vogtle,F.N., Teixeira,P.F., Imhof,A.,
            Meisinger,C. and Ott,M.
  TITLE     The novel component Kgd4 recruits the E3 subunit to the
            mitochondrial alpha-ketoglutarate dehydrogenase
  JOURNAL   Mol Biol Cell 25 (21), 3342-3349 (2014)
   PUBMED   25165143
REFERENCE   5  (bases 1 to 716)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC165159.2 and AK003765.1.
            
            On Jun 15, 2010 this sequence version replaced NM_025369.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: CN835714.1, AK003765.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-24                AC165159.2         201730-201753       c
            25-695              AK003765.1         2-672
            696-716             AC165159.2         193034-193054       c
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 53.23 cM"
     gene            1..716
                     /gene="Mrps36"
                     /gene_synonym="1110018B13Rik; Kgd4; S36mt"
                     /note="mitochondrial ribosomal protein S36"
                     /db_xref="GeneID:66128"
                     /db_xref="MGI:MGI:1913378"
     exon            1..130
                     /gene="Mrps36"
                     /gene_synonym="1110018B13Rik; Kgd4; S36mt"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..400
                     /gene="Mrps36"
                     /gene_synonym="1110018B13Rik; Kgd4; S36mt"
                     /note="isoform 1 is encoded by transcript variant 1; 28S
                     ribosomal protein S36, mitochondrial; alpha-ketoglutarate
                     dehydrogenase component 4; alpha-ketoglutarate
                     dehydrogenase subunit 4"
                     /codon_start=1
                     /product="alpha-ketoglutarate dehydrogenase component 4
                     isoform 1"
                     /protein_id="NP_079645.1"
                     /db_xref="CCDS:CCDS36767.1"
                     /db_xref="GeneID:66128"
                     /db_xref="MGI:MGI:1913378"
                     /translation="MGSKMASATRVVQVVKPHAPLIKFPNRRDKPKLSASEALGSAAL
                     PSHSSAISQHSKGSTSPDLLMHQGPPDTAEIIKSLPQKYRRKPMSQEEMEFIQRGGPE
                     "
     exon            131..194
                     /gene="Mrps36"
                     /gene_synonym="1110018B13Rik; Kgd4; S36mt"
                     /inference="alignment:Splign:2.1.0"
     exon            195..382
                     /gene="Mrps36"
                     /gene_synonym="1110018B13Rik; Kgd4; S36mt"
                     /inference="alignment:Splign:2.1.0"
     exon            383..716
                     /gene="Mrps36"
                     /gene_synonym="1110018B13Rik; Kgd4; S36mt"
                     /inference="alignment:Splign:2.1.0"
     regulatory      676..681
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mrps36"
                     /gene_synonym="1110018B13Rik; Kgd4; S36mt"
                     /note="hexamer: ATTAAA"
     polyA_site      697
                     /gene="Mrps36"
                     /gene_synonym="1110018B13Rik; Kgd4; S36mt"
                     /note="major polyA site"
ORIGIN      
        1 gacatcacac aggctccgcc ccacgctgcc cactccagat acttctgcag ctcgtctgcg
       61 ccccggaagc tgcccctgct cgggggtcac gatgggcagc aagatggctt ccgccaccag
      121 ggtcgtgcag gtagtgaagc cacacgctcc attaataaag ttccctaaca gaagagacaa
      181 acctaaactc agtgcctcag aagctttggg gtctgcagcg ctcccctccc actcctctgc
      241 aatttcacag cattctaaag gaagtacgtc ccccgattta ctgatgcacc aggggccacc
      301 agacactgca gaaattataa aatcattacc tcagaaatac agaaggaaac ctatgtctca
      361 agaggaaatg gaatttatcc agcgtggggg tccagagtga ccgtcgtggc tgctgcttgt
      421 caccagacag aagggttagc tttacaataa aggacttcca caatgtcaaa tgaaaagtca
      481 tatattaaac aaccttaata aatattctgt gagcagaatg aagtacagaa actccaggtg
      541 gtcacttgta tctcctacac tgtgtatctc ggtcagctgc cccacaagct gccctgtaca
      601 cttattaaag tctacatttt taaatgctag cctattaact tgctcttgat tatgaaatgt
      661 tcccagcgat gagctattaa aagcacatag tatctaataa atgtcccttt tctgtt
//
