LOCUS       NM_011885                749 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus mitochondrial ribosomal protein S12 (Mrps12),
            transcript variant 2, mRNA.
ACCESSION   NM_011885
VERSION     NM_011885.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 749)
  AUTHORS   Richman,T.R., Ermer,J.A., Siira,S.J., Kuznetsova,I., Brosnan,C.A.,
            Rossetti,G., Baker,J., Perks,K.L., Cserne Szappanos,H., Viola,H.M.,
            Gray,N., Larance,M., Hool,L.C., Zuryn,S., Rackham,O. and
            Filipovska,A.
  TITLE     Mitochondrial mistranslation modulated by metabolic stress causes
            cardiovascular disease and reduced lifespan
  JOURNAL   Aging Cell 20 (7), e13408 (2021)
   PUBMED   34096683
REFERENCE   3  (bases 1 to 749)
  AUTHORS   Cheong,A., Lingutla,R. and Mager,J.
  TITLE     Expression analysis of mammalian mitochondrial ribosomal protein
            genes
  JOURNAL   Gene Expr Patterns 38, 119147 (2020)
   PUBMED   32987154
REFERENCE   4  (bases 1 to 749)
  AUTHORS   Ferreira,N., Perks,K.L., Rossetti,G., Rudler,D.L., Hughes,L.A.,
            Ermer,J.A., Scott,L.H., Kuznetsova,I., Richman,T.R., Narayana,V.K.,
            Abudulai,L.N., Shearwood,A.J., Cserne Szappanos,H., Tull,D.,
            Yeoh,G.C., Hool,L.C., Filipovska,A. and Rackham,O.
  TITLE     Stress signaling and cellular proliferation reverse the effects of
            mitochondrial mistranslation
  JOURNAL   EMBO J 38 (24), e102155 (2019)
   PUBMED   31721250
REFERENCE   5  (bases 1 to 749)
  AUTHORS   Liu,X., Jiao,Y., Cao,Y., Deng,N., Ma,Y., Hasty,K.A., Kang,A.,
            Chen,H., Stuart,J.M. and Gu,W.
  TITLE     Decreased expression levels of Ifi genes is associated to the
            increased resistance to spontaneous arthritis disease in mice
            deficiency of IL-1RA
  JOURNAL   BMC Immunol 17 (1), 25 (2016)
   PUBMED   27480124
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 749)
  AUTHORS   Zanotto,E., Hakkinen,A., Teku,G., Shen,B., Ribeiro,A.S. and
            Jacobs,H.T.
  TITLE     NF-Y influences directionality of transcription from the
            bidirectional Mrps12/Sarsm promoter in both mouse and human cells
  JOURNAL   Biochim Biophys Acta 1789 (5), 432-442 (2009)
   PUBMED   19439209
  REMARK    GeneRIF: NF-Y type CCAAT boxes are found preferentially in
            bidirectional Mrps12/Sarsm promoters, but many such promoters lack
            them and must be regulated in another way.
REFERENCE   7  (bases 1 to 749)
  AUTHORS   Zanotto,E., Shah,Z.H. and Jacobs,H.T.
  TITLE     The bidirectional promoter of two genes for the mitochondrial
            translational apparatus in mouse is regulated by an array of CCAAT
            boxes interacting with the transcription factor NF-Y
  JOURNAL   Nucleic Acids Res 35 (2), 664-677 (2007)
   PUBMED   17179180
  REMARK    GeneRIF: Identification of an array of 4 CCAAT box elements
            required for efficient transcription of the Mrps12 gene.
REFERENCE   8  (bases 1 to 749)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   9  (bases 1 to 749)
  AUTHORS   Suzuki,T., Terasaki,M., Takemoto-Hori,C., Hanada,T., Ueda,T.,
            Wada,A. and Watanabe,K.
  TITLE     Proteomic analysis of the mammalian mitochondrial ribosome.
            Identification of protein components in the 28 S small subunit
  JOURNAL   J Biol Chem 276 (35), 33181-33195 (2001)
   PUBMED   11402041
REFERENCE   10 (bases 1 to 749)
  AUTHORS   Shah,Z.H., O'Dell,K.M., Miller,S.C., An,X. and Jacobs,H.T.
  TITLE     Metazoan nuclear genes for mitoribosomal protein S12
  JOURNAL   Gene 204 (1-2), 55-62 (1997)
   PUBMED   9434165
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC171210.1 and Y11682.1.
            
            On Jan 30, 2018 this sequence version replaced NM_011885.4.
            
            Transcript Variant: This variant (2) differs in the 5' UTR compared
            to variant 1. Variants 1, 2, and 3 all encode the same isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027522.1, Y11682.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                AC171210.1         148732-148772
            42-749              Y11682.1           1-708
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 16.91 cM"
     gene            1..749
                     /gene="Mrps12"
                     /gene_synonym="Rpms12; rps12"
                     /note="mitochondrial ribosomal protein S12"
                     /db_xref="GeneID:24030"
                     /db_xref="MGI:MGI:1346333"
     exon            1..57
                     /gene="Mrps12"
                     /gene_synonym="Rpms12; rps12"
                     /inference="alignment:Splign:2.1.0"
     exon            58..125
                     /gene="Mrps12"
                     /gene_synonym="Rpms12; rps12"
                     /inference="alignment:Splign:2.1.0"
     CDS             77..496
                     /gene="Mrps12"
                     /gene_synonym="Rpms12; rps12"
                     /note="isoform a precursor is encoded by transcript
                     variant 2; 28S ribosomal protein S12, mitochondrial;
                     S12mt; MRP-S12; MT-RPS12; mitoribosomal protein s12;
                     ribosomal protein, mitochondrial, S12; small ribosomal
                     subunit protein uS12m"
                     /codon_start=1
                     /product="small ribosomal subunit protein uS12m isoform a
                     precursor"
                     /protein_id="NP_036015.1"
                     /db_xref="CCDS:CCDS21052.1"
                     /db_xref="GeneID:24030"
                     /db_xref="MGI:MGI:1346333"
                     /translation="MSWPGLLYGLTTSLSRGLALAPQLWAARSMATLNQMHRLGPRKE
                     PPKRLGPTEGRPQLKGVVLRTFIRKPKKPNSANRKCCRVRLSTGKEAVCFIPGEGHTL
                     QEHHVVLVEGGRTQDLPGVKLKVVRGKYDCGHVQKKK"
     exon            126..724
                     /gene="Mrps12"
                     /gene_synonym="Rpms12; rps12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      696..701
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mrps12"
                     /gene_synonym="Rpms12; rps12"
                     /note="hexamer: AATAAA"
     polyA_site      724
                     /gene="Mrps12"
                     /gene_synonym="Rpms12; rps12"
                     /note="major polyA site"
ORIGIN      
        1 cccggaaggg gctggaggta ggggttggcg tgtgtgcgac cccgctaggt tggtgaggga
       61 cgctgcaggc ggcgagatgt cctggcccgg ccttctttat ggcctcacca cgtccctcag
      121 tcgtggccta gccctcgctc cccagctctg ggccgcccgt tccatggcca ccctgaacca
      181 gatgcaccgc ttaggccccc ggaaggaacc ccctaagcgt ctgggcccca cagagggccg
      241 gccccagctg aagggtgtgg tgttgcgcac attcatccga aagccgaaga agcccaactc
      301 cgccaaccgc aagtgctgcc gagtccgcct cagcacaggc aaagaggctg tctgcttcat
      361 ccccggggag ggccacaccc tgcaggagca tcacgtggtc ctcgtggagg gtggtcgcac
      421 ccaggacctg cctggggtca aactcaaagt tgtgaggggc aagtatgact gcggccacgt
      481 gcaaaagaag aaatgacagt aattcacagt ggtctggcac tcgatccttg gaaacgggtt
      541 cttcctactc ccgggtcttg ggctcaaatc ttactgtgat tcagcaagac tgttgttaac
      601 ctgcaagagt actgaaggtg aattggaagg cagtacccct gtgcgtccat gttcagtgtt
      661 tgcaggttga cctcatgtgc tgggatgggg tttgtaataa atatatcttc aggttgtgat
      721 gttaaaaaaa aaaaaaaaaa aaaaaaaaa
//
