LOCUS       NM_023167               1312 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus mitochondrial ribosomal protein L4 (Mrpl4), transcript
            variant 2, mRNA.
ACCESSION   NM_023167
VERSION     NM_023167.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Cheong,A., Lingutla,R. and Mager,J.
  TITLE     Expression analysis of mammalian mitochondrial ribosomal protein
            genes
  JOURNAL   Gene Expr Patterns 38, 119147 (2020)
   PUBMED   32987154
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Liu,H.B., Muhammad,T., Guo,Y., Li,M.J., Sha,Q.Q., Zhang,C.X.,
            Liu,H., Zhao,S.G., Zhao,H., Zhang,H., Du,Y.Z., Sun,K., Liu,K.,
            Lu,G., Guo,X.J., Sha,J., Fan,H.Y., Gao,F. and Chen,Z.J.
  TITLE     RNA-Binding Protein IGF2BP2/IMP2 is a Critical Maternal Activator
            in Early Zygotic Genome Activation
  JOURNAL   Adv Sci (Weinh) 6 (15), 1900295 (2019)
   PUBMED   31406667
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1312)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   4  (bases 1 to 1312)
  AUTHORS   Brown,A., Amunts,A., Bai,X.C., Sugimoto,Y., Edwards,P.C.,
            Murshudov,G., Scheres,S.H.W. and Ramakrishnan,V.
  TITLE     Structure of the large ribosomal subunit from human mitochondria
  JOURNAL   Science 346 (6210), 718-722 (2014)
   PUBMED   25278503
REFERENCE   5  (bases 1 to 1312)
  AUTHORS   Hoffman,B.G., Zavaglia,B., Witzsche,J., Ruiz de Algara,T.,
            Beach,M., Hoodless,P.A., Jones,S.J., Marra,M.A. and Helgason,C.D.
  TITLE     Identification of transcripts with enriched expression in the
            developing and adult pancreas
  JOURNAL   Genome Biol 9 (6), R99 (2008)
   PUBMED   18554416
REFERENCE   6  (bases 1 to 1312)
  AUTHORS   Suzuki,T., Terasaki,M., Takemoto-Hori,C., Hanada,T., Ueda,T.,
            Wada,A. and Watanabe,K.
  TITLE     Structural compensation for the deficit of rRNA with proteins in
            the mammalian mitochondrial ribosome. Systematic analysis of
            protein components of the large ribosomal subunit from mammalian
            mitochondria
  JOURNAL   J Biol Chem 276 (24), 21724-21736 (2001)
   PUBMED   11279069
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC159314.3, AK002464.1 and BC051685.1.
            
            On Dec 16, 2006 this sequence version replaced NM_023167.1.
            
            Transcript Variant: This variant (2) uses two alternate splice
            junctions compared to variant 1. The resulting isoform (2) has the
            same N- and C-termini but is shorter compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC051685.1, AB049634.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-165               AC159314.3         152827-152991
            166-1277            AK002464.1         2-1113
            1278-1312           BC051685.1         1091-1125
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 7.69 cM"
     gene            1..1312
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /note="mitochondrial ribosomal protein L4"
                     /db_xref="GeneID:66163"
                     /db_xref="MGI:MGI:2137210"
     exon            1..261
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..1089
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /EC_number="3.6.5.3"
                     /note="isoform 2 is encoded by transcript variant 2; 39S
                     ribosomal protein L4, mitochondrial; L4mt; MRP-L4; large
                     ribosomal subunit protein uL4m"
                     /codon_start=1
                     /product="large ribosomal subunit protein uL4m isoform 2"
                     /protein_id="NP_075656.2"
                     /db_xref="CCDS:CCDS40548.1"
                     /db_xref="GeneID:66163"
                     /db_xref="MGI:MGI:2137210"
                     /translation="MLRLFQAASRASLRLSGSRVIHSLAEGAERPAEISEPRDSAGLL
                     DPVLRKCELRIPVHRRPVQAWVESLRGFEQERIGLAELHPDVFATAPRLDIVHQVAIW
                     QRNFRRISYANTKTRAEVSGGGRKPWQQKGSGRARHGSIRSPLWRGGGVAHGPRGPTS
                     YYYMLPMKVRALGLKVALTVKLMQDDLHIVDSLELPTADPQYLTELAQYRHWGSSVLL
                     VDLTHEEMPKNVVAATSGLNSFNLIPAVGLNVYSMLKHQTLVLTLPSVAFLEDKLLWQ
                     DSRYTPLYPFRLPYSDFP"
     exon            262..328
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            329..479
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            480..531
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            532..649
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            650..756
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            757..866
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            867..943
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            944..1289
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1259..1264
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1281
                     /gene="Mrpl4"
                     /gene_synonym="1110017G11Rik"
                     /note="major polyA site"
ORIGIN      
        1 gcttcccggc acccggctct ggctctccgg tgtcggggct tccggctgtc aagtggagct
       61 gatccacagc ctcaggcttc gggacctggc gctgatggcg gcggaatcgc cttcgtgacg
      121 tacccggcgc acatacgacg tcatcagcca ccgccgcgag cgtccgtacc gcagcgacct
      181 cggcgctgcg agaggtcctc cgagatgctg cggttgttcc aggccgcttc ccgggcctcg
      241 cttcgactct ctggctcccg ggtcatccac tctctagcgg aaggcgcgga gcggccggcc
      301 gagatttcag agccgcgaga tagcgctggt ctcctagatc ctgttttgcg caagtgtgag
      361 ctccggatcc cagtgcaccg gcgtccggtg caagcctggg tcgagtctct ccgaggcttt
      421 gagcaggagc gcatcggcct ggcggagctg caccccgacg tgtttgcaac ggcgcctcgg
      481 ctggacattg tgcaccaggt cgccatctgg cagaggaact tcaggagaat cagctatgct
      541 aacaccaaga ccagggctga ggtgagtggt ggtggccgca agccctggca acagaaaggc
      601 agtggccgtg ctcggcatgg cagcatccgt tctcccttat ggcgaggagg aggtgtagcc
      661 catggtcccc ggggtcctac aagttactac tacatgttgc ccatgaaagt gcgggcactg
      721 ggcctcaagg tggccttgac tgtcaagctc atgcaggatg atctacacat tgtggattcc
      781 ctggagctac ccacggcaga cccccagtac ctgacagagt tggcccagta ccgccactgg
      841 gggagttccg tgctccttgt agacttaaca catgaagaga tgcccaagaa tgtcgtggca
      901 gccacttcag ggctcaacag tttcaacctg atccccgcag ttggcctgaa tgtgtacagc
      961 atgctcaagc accagactct ggtcctcacc ctgccttccg tcgccttcct ggaggacaag
     1021 ctactctggc aggactcaag atacactccg ctctacccct tccgcttgcc ctacagtgac
     1081 ttcccctaac cctgccctgg tcacctaggg acctgcccca cttgggggcc aagtcagcac
     1141 cccagccatc ctgcaaaaac agcagttccc tgggagctgt tggaccttgt tgcaaggagg
     1201 ggacaactgc acagagccaa gatgagtgcc tgcctgggat ctcttttgtc acattaaaaa
     1261 taaaccctat ttttcacaag acttgaaaaa aaaaaaaaaa aaaaaaaaaa aa
//
