LOCUS       NM_027204                998 bp    mRNA    linear   ROD 09-FEB-2025
DEFINITION  Mus musculus mitochondrial ribosomal protein L12 (Mrpl12), mRNA.
ACCESSION   NM_027204
VERSION     NM_027204.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 998)
  AUTHORS   Ji,X., Chu,L., Su,D., Sun,J., Song,P., Sun,S., Wang,Y., Mu,Q.,
            Liu,Y. and Wan,Q.
  TITLE     MRPL12-ANT3 interaction involves in acute kidney injury via
            regulating MPTP of tubular epithelial cells
  JOURNAL   iScience 26 (5), 106656 (2023)
   PUBMED   37182101
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 998)
  AUTHORS   Cheong,A., Lingutla,R. and Mager,J.
  TITLE     Expression analysis of mammalian mitochondrial ribosomal protein
            genes
  JOURNAL   Gene Expr Patterns 38, 119147 (2020)
   PUBMED   32987154
REFERENCE   4  (bases 1 to 998)
  AUTHORS   Greber,B.J. and Ban,N.
  TITLE     Structure and Function of the Mitochondrial Ribosome
  JOURNAL   Annu Rev Biochem 85, 103-132 (2016)
   PUBMED   27023846
  REMARK    Review article
REFERENCE   5  (bases 1 to 998)
  AUTHORS   Nouws,J., Goswami,A.V., Bestwick,M., McCann,B.J., Surovtseva,Y.V.
            and Shadel,G.S.
  TITLE     Mitochondrial Ribosomal Protein L12 Is Required for POLRMT
            Stability and Exists as Two Forms Generated by Alternative
            Proteolysis during Import
  JOURNAL   J Biol Chem 291 (2), 989-997 (2016)
   PUBMED   26586915
  REMARK    GeneRIF: knock-down of MRPL12 by RNA interference results in
            instability of POLRMT.
REFERENCE   6  (bases 1 to 998)
  AUTHORS   Brown,A., Amunts,A., Bai,X.C., Sugimoto,Y., Edwards,P.C.,
            Murshudov,G., Scheres,S.H.W. and Ramakrishnan,V.
  TITLE     Structure of the large ribosomal subunit from human mitochondria
  JOURNAL   Science 346 (6210), 718-722 (2014)
   PUBMED   25278503
REFERENCE   7  (bases 1 to 998)
  AUTHORS   Taniwaki,T., Haruna,K., Nakamura,H., Sekimoto,T., Oike,Y.,
            Imaizumi,T., Saito,F., Muta,M., Soejima,Y., Utoh,A., Nakagata,N.,
            Araki,M., Yamamura,K. and Araki,K.
  TITLE     Characterization of an exchangeable gene trap using pU-17 carrying
            a stop codon-beta geo cassette
  JOURNAL   Dev Growth Differ 47 (3), 163-172 (2005)
   PUBMED   15840001
REFERENCE   8  (bases 1 to 998)
  AUTHORS   Cobellis,G., Nicolaus,G., Iovino,M., Romito,A., Marra,E.,
            Barbarisi,M., Sardiello,M., Di Giorgio,F.P., Iovino,N., Zollo,M.,
            Ballabio,A. and Cortese,R.
  TITLE     Tagging genes with cassette-exchange sites
  JOURNAL   Nucleic Acids Res 33 (4), e44 (2005)
   PUBMED   15741177
  REMARK    Publication Status: Online-Only
REFERENCE   9  (bases 1 to 998)
  AUTHORS   Graack,H.R., Bryant,M.L. and O'Brien,T.W.
  TITLE     Identification of mammalian mitochondrial ribosomal proteins (MRPs)
            by N-terminal sequencing of purified bovine MRPs and comparison to
            data bank sequences: the large subribosomal particle
  JOURNAL   Biochemistry 38 (50), 16569-16577 (1999)
   PUBMED   10600119
REFERENCE   10 (bases 1 to 998)
  AUTHORS   Araki,K., Imaizumi,T., Sekimoto,T., Yoshinobu,K., Yoshimuta,J.,
            Akizuki,M., Miura,K., Araki,M. and Yamamura,K.
  TITLE     Exchangeable gene trap using the Cre/mutated lox system
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 45 (5), 737-750 (1999)
   PUBMED   10512203
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK134816.1 and BQ032019.1.
            
            On Mar 1, 2018 this sequence version replaced NM_027204.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC019565.1, AK134816.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-914               AK134816.1         2-915
            915-998             BQ032019.1         1-84                c
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 84.18 cM"
     gene            1..998
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /note="mitochondrial ribosomal protein L12"
                     /db_xref="GeneID:56282"
                     /db_xref="MGI:MGI:1926273"
     exon            1..132
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /inference="alignment:Splign:2.1.0"
     CDS             56..661
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /note="39S ribosomal protein L12, mitochondrial; large
                     ribosomal subunit protein bL12m"
                     /codon_start=1
                     /product="large ribosomal subunit protein bL12m precursor"
                     /protein_id="NP_081480.2"
                     /db_xref="CCDS:CCDS25737.1"
                     /db_xref="GeneID:56282"
                     /db_xref="MGI:MGI:1926273"
                     /translation="MLPVAASRCLWGPRLGLRGAALRLARQQMPSVCAARQLRSSSHR
                     RSEALAGAPLDNAPKEYPPKIQQLVQDIASLTLLEISDLNELLKKTLKIQDVGLMPMG
                     GMVPGPVSAAAPASEAAEEEDVPKQKERTHFTVRLTEAKPVDKVKLIKEIKNYVQGIN
                     LVQAKKLVESLPQEIKANVAKAEAEKIKAALEAVGGTVVLE"
     exon            133..319
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /inference="alignment:Splign:2.1.0"
     exon            320..406
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /inference="alignment:Splign:2.1.0"
     exon            407..544
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /inference="alignment:Splign:2.1.0"
     exon            545..981
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      903..908
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /note="hexamer: AATATA"
     polyA_site      930
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /note="major polyA site"
     regulatory      960..965
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /note="hexamer: TATAAA"
     polyA_site      981
                     /gene="Mrpl12"
                     /gene_synonym="0610034O11Rik; 1500031N16Rik; L12mt;
                     MRP-L12; Rpml12"
                     /note="major polyA site"
ORIGIN      
        1 ggatagagcg tcgcttccgc ttagagccgc gtgtgacctt cccgcttgcg gagctatgct
       61 gccggtggcg gctagccgct gcctgtgggg gccacggctt ggactccggg gggcagcgct
      121 tcgccttgcc aggcaacaga tgcccagcgt ctgtgcagcg cggcagttga gaagcagcag
      181 ccaccgaagg agtgaagcac tcgccggcgc acctctggat aatgctccca aagaataccc
      241 ccccaagatc cagcagctgg tccaagacat tgccagcctt acgctcctgg agatctcaga
      301 cctcaacgaa ctcctgaaga aaacattgaa gatccaggat gtcggcctta tgccaatggg
      361 tggcatggtg cctggtcccg tctctgctgc agcaccggcc tctgaggcgg ccgaggagga
      421 agacgtcccc aaacagaaag agcggacaca cttcaccgtc cgcctgacag aggcaaagcc
      481 tgtggacaaa gtaaagctga tcaaggagat caagaactac gtccagggca tcaacctcgt
      541 ccaggccaag aagctggtag agtccctgcc ccaggaaatc aaagccaacg tcgccaaagc
      601 tgaggccgag aagatcaaag cagccttgga ggcagtgggt ggcaccgtgg ttctggagtg
      661 aggcatggtt atgaggactt acggactaga gttctgggat ccgggcaaag cctgctcacc
      721 ccgattcact cccagctgct gtggtgggag caacagctag tgacgcgagt gtccgaagtc
      781 tcctgagagg gacaggatgg acctactgtg atggggtggg gcagtgacaa cctttgcaga
      841 actcacaaga gattgccttg ggagcttacc aagtgaacac accctctggt ggctactgag
      901 aaaatatact attcaggctg gggtgttcaa tgagtggtgt gtgtgcccag ggctgggagt
      961 ataaaggacc acctggaggc aaaaaaaaaa aaaaaaaa
//
