LOCUS       NM_001034868            4559 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus MAS-related GPR, member X2 (Mrgprx2), mRNA.
ACCESSION   NM_001034868 XM_145611 XM_915388
VERSION     NM_001034868.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Zhang,Y., Bai,H., Zhang,W., Gao,J., Gao,C., Deng,T., Liu,X.,
            Sun,X., Liu,Y., Wang,N. and Wu,Y.
  TITLE     miR-212/132 attenuates OVA-induced airway inflammation by
            inhibiting mast cells activation through MRGPRX2 and ASAP1
  JOURNAL   Exp Cell Res 433 (2), 113828 (2023)
   PUBMED   37875175
  REMARK    GeneRIF: miR-212/132 attenuates OVA-induced airway inflammation by
            inhibiting mast cells activation through MRGPRX2 and ASAP1.
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Sbei,S., Moncrief,T., Limjunyawong,N., Zeng,Y. and Green,D.P.
  TITLE     PACAP activates MRGPRX2 on meningeal mast cells to drive
            migraine-like pain
  JOURNAL   Sci Rep 13 (1), 12302 (2023)
   PUBMED   37516794
  REMARK    GeneRIF: PACAP activates MRGPRX2 on meningeal mast cells to drive
            migraine-like pain.
            Erratum:[Sci Rep. 2023 Aug 16;13(1):13298. doi:
            10.1038/s41598-023-40309-z.. PMID: 37587209]
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4559)
  AUTHORS   Kunimura,K., Akiyoshi,S., Uruno,T., Matsubara,K., Sakata,D.,
            Morino,K., Hirotani,K. and Fukui,Y.
  TITLE     DOCK2 regulates MRGPRX2/B2-mediated mast cell degranulation and
            drug-induced anaphylaxis
  JOURNAL   J Allergy Clin Immunol 151 (6), 1585-1594 (2023)
   PUBMED   36804596
  REMARK    GeneRIF: DOCK2 regulates MRGPRX2/B2-mediated mast cell
            degranulation and drug-induced anaphylaxis.
REFERENCE   4  (bases 1 to 4559)
  AUTHORS   Iio,K., Kutsumura,N., Nagumo,Y., Saitoh,T., Tokuda,A.,
            Hashimoto,K., Yamamoto,N., Kise,R., Inoue,A., Mizoguchi,H. and
            Nagase,H.
  TITLE     Synthesis of unnatural morphinan compounds to induce itch-like
            behaviors in mice: Towards the development of MRGPRX2 selective
            ligands
  JOURNAL   Bioorg Med Chem Lett 56, 128485 (2022)
   PUBMED   34861349
  REMARK    GeneRIF: Synthesis of unnatural morphinan compounds to induce
            itch-like behaviors in mice: Towards the development of MRGPRX2
            selective ligands.
REFERENCE   5  (bases 1 to 4559)
  AUTHORS   Lazki-Hagenbach,P., Ali,H. and Sagi-Eisenberg,R.
  TITLE     Authentic and Ectopically Expressed MRGPRX2 Elicit Similar
            Mechanisms to Stimulate Degranulation of Mast Cells
  JOURNAL   Cells 10 (2), 376 (2021)
   PUBMED   33673037
  REMARK    GeneRIF: Authentic and Ectopically Expressed MRGPRX2 Elicit Similar
            Mechanisms to Stimulate Degranulation of Mast Cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4559)
  AUTHORS   Liu,R., Che,D., Zhao,T., Pundir,P., Cao,J., Lv,Y., Wang,J., Ma,P.,
            Fu,J., Wang,N., Wang,X., Zhang,T., Dong,X. and He,L.
  TITLE     MRGPRX2 is essential for sinomenine hydrochloride induced
            anaphylactoid reactions
  JOURNAL   Biochem Pharmacol 146, 214-223 (2017)
   PUBMED   28987593
  REMARK    GeneRIF: The results showed a kind of mechanism that
            sinomenine-induced anaphylactoid reactions were mediated by MRGPRX2
            via activating PLC molecular signaling pathways to provoke mast
            cells Ca(2+) mobilization and degranulation.
REFERENCE   7  (bases 1 to 4559)
  AUTHORS   McNeil,B.D., Pundir,P., Meeker,S., Han,L., Undem,B.J., Kulka,M. and
            Dong,X.
  TITLE     Identification of a mast-cell-specific receptor crucial for
            pseudo-allergic drug reactions
  JOURNAL   Nature 519 (7542), 237-241 (2015)
   PUBMED   25517090
REFERENCE   8  (bases 1 to 4559)
  AUTHORS   Zylka,M.J., Dong,X., Southwell,A.L. and Anderson,D.J.
  TITLE     Atypical expansion in mice of the sensory neuron-specific Mrg G
            protein-coupled receptor family
  JOURNAL   Proc Natl Acad Sci U S A 100 (17), 10043-10048 (2003)
   PUBMED   12909716
REFERENCE   9  (bases 1 to 4559)
  AUTHORS   Han,S.K., Dong,X., Hwang,J.I., Zylka,M.J., Anderson,D.J. and
            Simon,M.I.
  TITLE     Orphan G protein-coupled receptors MrgA1 and MrgC11 are
            distinctively activated by RF-amide-related peptides through the
            Galpha q/11 pathway
  JOURNAL   Proc Natl Acad Sci U S A 99 (23), 14740-14745 (2002)
   PUBMED   12397184
REFERENCE   10 (bases 1 to 4559)
  AUTHORS   Dong,X., Han,S., Zylka,M.J., Simon,M.I. and Anderson,D.J.
  TITLE     A diverse family of GPCRs expressed in specific subsets of
            nociceptive sensory neurons
  JOURNAL   Cell 106 (5), 619-632 (2001)
   PUBMED   11551509
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ131827.1, AK148121.1, AK148319.1 and AC115742.13.
            
            On May 20, 2008 this sequence version replaced NM_001034868.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK148121.1, BC141225.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-72                CJ131827.1         52-123
            73-715              AK148121.1         4-646
            716-1809            AK148319.1         37-1130
            1810-2311           AK148121.1         1741-2242
            2312-2552           AK148319.1         1632-1872
            2553-2672           AK148121.1         2484-2603
            2673-2673           AC115742.13        151491-151491       c
            2674-3059           AK148121.1         2604-2989
            3060-3060           AC115742.13        151104-151104       c
            3061-4559           AK148121.1         2990-4488
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 31.11 cM"
     gene            1..4559
                     /gene="Mrgprx2"
                     /gene_synonym="G370024M05Rik; MrgB10; Mrgprb10"
                     /note="MAS-related GPR, member X2"
                     /db_xref="GeneID:243978"
                     /db_xref="MGI:MGI:3588270"
     exon            1..120
                     /gene="Mrgprx2"
                     /gene_synonym="G370024M05Rik; MrgB10; Mrgprb10"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..1168
                     /gene="Mrgprx2"
                     /gene_synonym="G370024M05Rik; MrgB10; Mrgprb10"
                     /note="mas-related G-protein coupled receptor member B10;
                     MAS-related GPR, member B10"
                     /codon_start=1
                     /product="mas-related G-protein coupled receptor member
                     X2"
                     /protein_id="NP_001030040.2"
                     /db_xref="CCDS:CCDS52258.1"
                     /db_xref="GeneID:243978"
                     /db_xref="MGI:MGI:3588270"
                     /translation="MEERNISGRDLRVDSNITYWGTNITAVNESNHTGMSFCEVVSCT
                     MVFLSLIVALVGLVGNATVLWFLGFQMRRNAFSVYILNLAGADFLFICFQIGYCFHMI
                     LDIDSIPIEIDLFYLVVLNFPYFCGLSILSAISIERCLSVMWPIWYHCQRPRHTSAVI
                     CTLLWVLSLVCSLLEGKECGFLYYTSDPGWCKTFDLITATWLIVLFVALLGSSLALVI
                     TIFWGLHKIPVTRLYVAIVFTVLVFLLFGLPYGIYWFLLVWIEKFYYVLPCSIYPVTV
                     FLSCVNSSAKPIIYCLVGSIRHHRFQRKTLKLFLQRAMQDTPEEEECGEMGSSGRSRE
                     IKTIWKGLRAALIRHKEL"
     exon            121..4559
                     /gene="Mrgprx2"
                     /gene_synonym="G370024M05Rik; MrgB10; Mrgprb10"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4543..4548
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mrgprx2"
                     /gene_synonym="G370024M05Rik; MrgB10; Mrgprb10"
                     /note="putative"
     polyA_site      4559
                     /gene="Mrgprx2"
                     /gene_synonym="G370024M05Rik; MrgB10; Mrgprb10"
                     /note="putative"
ORIGIN      
        1 acactggtca cattgccaac catgcactac agacatgttt catattgaca gctgattcca
       61 gaaactcttc actttgacac agaccaattt aacatctccc ataagaaaaa tggaggaaag
      121 aaacatcagt ggaagggatc taagagtgga ttcaaacata acatactggg gaacaaacat
      181 cacagctgtg aatgaaagca accatactgg aatgtcattt tgtgaagtcg tgtcttgtac
      241 catggttttt ctttccctca ttgttgccct agttgggctg gttggaaatg ccacagtgct
      301 gtggttcctg ggcttccaga tgcgcaggaa tgcattctct gtttacatcc tcaacctcgc
      361 tggtgctgac tttctcttca tttgctttca aattggatat tgttttcaca tgatcttgga
      421 cattgattcc atccccattg aaattgatct gttttacctt gttgtgttaa actttcctta
      481 tttttgtggc ctgagtatcc tcagtgctat tagcattgaa cgttgcctgt ctgtcatgtg
      541 gcccatttgg tatcactgcc aacgcccaag gcacacatca gctgtcatat gtaccctgct
      601 ttgggtcttg tccctagtgt gtagcctcct ggaagggaag gaatgtggct tcctatatta
      661 cactagtgac cctggttggt gtaagacatt tgatttaatc actgctacat ggttaattgt
      721 tttatttgta gctctcttgg gatccagtct ggccttagtg attaccatct tctggggctt
      781 acacaagatt cctgtgacca ggctgtatgt ggccattgtg ttcacagtgc ttgttttcct
      841 gctctttggt ctgccctatg ggatctactg gttcctctta gtgtggattg agaaatttta
      901 ttatgtttta ccttgtagta tatatccggt cacagtattt ctctcctgtg ttaacagctc
      961 tgcaaaaccc atcatttatt gccttgtagg ctccattagg catcatcgat ttcaacggaa
     1021 gactctcaag ctatttctgc agagagccat gcaagacact cctgaggagg aagaatgtgg
     1081 agagatgggt tcctcaggaa gatctagaga aataaaaaca atctggaaag gactgagagc
     1141 tgctttgatc aggcataaag agctctgaag agaactatgt ttttatcact ttgttgcatt
     1201 ttcataacgt tgtttagttg atgacccaag gttaactcag ttgggaaagt agtcaatgtt
     1261 gtagaagttg attgatattg gacttgttac aaatactggg tacaacattt ctgcagctat
     1321 cttgctcagg gttttaccaa cttcttttga tgttactcct tgcaagctct gtggggtcca
     1381 ggaaagctgt tgaccacaat tgataaatcc cttcttcaga agaaagctta agaaagtaca
     1441 ggaaagggtt gcatttctta actcacttaa cttgatagtg gataaattca tgttatattt
     1501 tgcaaaaaaa ttattctgtt tcaaggcaaa cttttcttca gtgttgaagg gttaaataga
     1561 tacattatat aatcccagac tttattaatt tctgtatgtt ttaaagaata tgtggagcaa
     1621 tagtttttct tatacacatt tcttaataaa gaagtaaaca ttctcaagag aagtgttaaa
     1681 catccatgta cataggaagg tgcagtgtcc tctgtggttc tattcacagt ttccttttta
     1741 gcatcccata gttgagtatt gtctttgata tgatcctcat gctctctgac tgtgtaatcc
     1801 ctcatgaaaa gtttccaatg aggtcctcta taaagactcc cttgaaatac aacttatttt
     1861 aaatttatac catttcaagg agcccacagc atctattaac ttagctatat gcacagttta
     1921 gtaaaatttt ctataaaata atattccttt tataaagctg cagtaataat ttcaattttt
     1981 ctacaattaa gagaataaaa tatcaacaaa ttaaataaaa ctaatcagta ggttttctta
     2041 agttaatgta gctgcatgac tctgtaccta atcaagacac aaaatactac actatatctt
     2101 ttaattttca tttcttctcc tgtcataatt ttatatcaca gataaatatg atatccatac
     2161 ttctgttcta aactaaatat tagagtgaaa attaaaagat tgtaacatga tgcttgatac
     2221 atgtggctag atggttgttg tcgttgaaca tactaaccta tacatcaggt catgtaaaca
     2281 ttcatttttt attccttgtg tcaaaaactt cgttttcaca aaatctcctg tgtgcaatga
     2341 actattacta caaagatctc atgctttaga ttacttatat agtttgaatg aaaatggcta
     2401 ccataggctt acccctaatt gttggaattg tttgggcagg attaggacct gtggccttgt
     2461 ttgaggaggt atgacactcc gggttgcaaa tttgaggttg caaatcccca atgcttccac
     2521 ccacacccca tgtggaagta taagtattaa atataagctc tcaactacta ctccagtgtc
     2581 atagctgcct gcttactgtc atactgtcat actttcagct atggtggttg tgcagtacaa
     2641 attccgggac tgtgatctac caaattaagt gctttttttt tttcttgtaa attgcctttt
     2701 tcatgccttt ttatcacagc aatggaaata tatctaaaac agaagctggt atcagagaca
     2761 gtgctattta tgtaacaggc atgatcctat tgcttttatg gggagggacc aagagtatgg
     2821 gattttggat tagggatgtg attgatgtag ggcttaacag accatcctag aaggaatatg
     2881 gatgaaagtg attctgaaag cattgtacat tatggagacc cagttcacat agtttttgct
     2941 ggaaaaaatt ttagtaactg agctaaaaac tattcttgga aatttgtcac aaaatatggc
     3001 tgctttttac ccttctccta agaatatgct ttaggctaaa ttgaaaagtt cgtaaataat
     3061 tttttttttt ttttggcaag ggatactagg tttacagtaa aaatgagtaa gaagggcaaa
     3121 aggaaatata aaatgtacaa ttgaagagaa aaggagaagc aggattttta attttggagc
     3181 taattcttgg gtttaaagaa gtgattagtt taaagaaggt cctgatggga aatggaataa
     3241 atgagattct gctctaggcc aagacaccac ccagctaagc tttcaacttg cacaggggaa
     3301 atgcataaag attttcctca agcaaaggag aatttgttca gcaaattaaa gcttatgcca
     3361 atgtaattca aggaagtgaa gatgtatgat ctgtgcctct gtatgtggga aacatgtact
     3421 tgacagttca acactgagca gacaacagaa gttggtagca tcagtcttct ggttctggcc
     3481 tttgagtcaa gaaggatgca ggagtaaagg gattatagaa tcttcctcca aggttaagaa
     3541 aagtgaatga ggacagacat gtaagcaggg agtccttgct tggaggcctg gaaatgtctc
     3601 aattgtgaca ttgtgacttt gtgacatgac attgtgaaga tgaagcctag attttactgg
     3661 agatggaaag atattggaga tgccaaaagt atggcatatc tgacaagaag cactgtatag
     3721 agagagtgaa acaagtccaa gagagagaaa tgtattgcag ttgaaaaggg tactacagag
     3781 tcatctcagc attttgacac caagacaaga agctacagga tttagaattt gccctactga
     3841 atttttattt tacttggccc aatatttcct cattatgtcc ccattcctct cttttgaaat
     3901 ggatggtaat gtataatctc tgactctgta tgttggaaaa atgtgctttg ctttttgttt
     3961 tgagaaggag ttacaattga aagattgtct tgagtctcat aagagacttt ggacttttaa
     4021 actgtactgt gaccaggaaa gactgcagac ttttcaagtt ggacttaatg tattttctcc
     4081 tgtgctatag ctacaaaatt atgtggtctg aggactgtaa tgtggtagtt tacccaaaaa
     4141 tgttccccta tagtctcatt ttggaaatgc ttggccccag gttggtggaa ctgtttggga
     4201 tagattaaaa tgagtgctct tgttggaata tatctcacta ggggtgatct ttgaggtttc
     4261 acaagccagt actattccca gttagatctt tatctgcctc aagcttgagg gtcagatatg
     4321 agcttccagc aactgcccca ttgccatggc aacctgcttg cctactgtca ggcttctggc
     4381 tatgatggtc atggattcca ctccacagga attgtgatcc tccctatgaa atatattttt
     4441 atgagtagtc tttgtcatca tggcaacaga aaagtaatta tgactctttc ttttatttta
     4501 tattttgtta atatttttaa aactatgttt ttcttctgtc taaataaaga cttgatgag
//
