LOCUS       NM_001177731            2093 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus melanocortin 2 receptor accessory protein 2 (Mrap2),
            transcript variant 1, mRNA.
ACCESSION   NM_001177731
VERSION     NM_001177731.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Bernard,A., Ojeda Naharros,I., Yue,X., Mifsud,F., Blake,A.,
            Bourgain-Guglielmetti,F., Ciprin,J., Zhang,S., McDaid,E., Kim,K.,
            Nachury,M.V., Reiter,J.F. and Vaisse,C.
  TITLE     MRAP2 regulates energy homeostasis by promoting primary cilia
            localization of MC4R
  JOURNAL   JCI Insight 8 (2), e155900 (2023)
   PUBMED   36692018
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2093)
  AUTHORS   Fullone,M.R., Maftei,D., Vincenzi,M., Lattanzi,R. and Miele,R.
  TITLE     Arginine 125 Is an Essential Residue for the Function of MRAP2
  JOURNAL   Int J Mol Sci 23 (17), 9853 (2022)
   PUBMED   36077245
  REMARK    GeneRIF: Arginine 125 Is an Essential Residue for the Function of
            MRAP2.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2093)
  AUTHORS   Wang,M., Zhai,Y., Lei,X., Xu,J., Jiang,B., Kuang,Z., Zhang,C.,
            Liu,S., Bian,S., Yang,X.M., Zan,T., Jin,L.N., Li,Q. and Zhang,C.
  TITLE     Determination of the Interaction and Pharmacological Modulation of
            MCHR1 Signaling by the C-Terminus of MRAP2 Protein
  JOURNAL   Front Endocrinol (Lausanne) 13, 848728 (2022)
   PUBMED   35311242
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2093)
  AUTHORS   Wang,M., Pi,L., Lei,X., Li,L., Xu,J., Kuang,Z., Zhang,C., Li,L. and
            Zhang,C.
  TITLE     Functional Characterization of the Internal Symmetry of MRAP2
            Antiparallel Homodimer
  JOURNAL   Front Endocrinol (Lausanne) 12, 750797 (2021)
   PUBMED   34759891
  REMARK    GeneRIF: Functional Characterization of the Internal Symmetry of
            MRAP2 Antiparallel Homodimer.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2093)
  AUTHORS   Srisai,D., Yin,T.C., Lee,A.A., Rouault,A.A.J., Pearson,N.A.,
            Grobe,J.L. and Sebag,J.A.
  TITLE     MRAP2 regulates ghrelin receptor signaling and hunger sensing
  JOURNAL   Nat Commun 8 (1), 713 (2017)
   PUBMED   28959025
  REMARK    GeneRIF: MRAP2 is an important modulator of the energy homeostasis
            machinery that operates through the regulation of multiple GPCRs
            throughout the hypothalamus.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2093)
  AUTHORS   Novoselova,T.V., Larder,R., Rimmington,D., Lelliott,C., Wynn,E.H.,
            Gorrigan,R.J., Tate,P.H., Guasti,L., O'Rahilly,S., Clark,A.J.,
            Logan,D.W., Coll,A.P. and Chan,L.F.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Loss of Mrap2 is associated with Sim1 deficiency and increased
            circulating cholesterol
  JOURNAL   J Endocrinol 230 (1), 13-26 (2016)
   PUBMED   27106110
  REMARK    GeneRIF: Mrap2 KO mice have a significant increase in body weight,
            with increased fat and lean mass, but without detectable changes in
            food intake or energy expenditure. Transcriptomic analysis showed
            significantly decreased expression of Sim1, Trh, Oxt and Crh within
            the paraventricular nucleus of Mrap2 KO mice. Circulating levels of
            both high-density and low-density lipoprotein were increased in
            Mrap2 KO mice.
REFERENCE   8  (bases 1 to 2093)
  AUTHORS   Chaly,A.L., Srisai,D., Gardner,E.E. and Sebag,J.A.
  TITLE     The Melanocortin Receptor Accessory Protein 2 promotes food intake
            through inhibition of the Prokineticin Receptor-1
  JOURNAL   Elife 5, e12397 (2016)
   PUBMED   26829592
  REMARK    GeneRIF: show that MRAP2 significantly and specifically inhibits
            PKR1 signaling.
            Publication Status: Online-Only
REFERENCE   9  (bases 1 to 2093)
  AUTHORS   Asai,M., Ramachandrappa,S., Joachim,M., Shen,Y., Zhang,R.,
            Nuthalapati,N., Ramanathan,V., Strochlic,D.E., Ferket,P.,
            Linhart,K., Ho,C., Novoselova,T.V., Garg,S., Ridderstrale,M.,
            Marcus,C., Hirschhorn,J.N., Keogh,J.M., O'Rahilly,S., Chan,L.F.,
            Clark,A.J., Farooqi,I.S. and Majzoub,J.A.
  TITLE     Loss of function of the melanocortin 2 receptor accessory protein 2
            is associated with mammalian obesity
  JOURNAL   Science 341 (6143), 275-278 (2013)
   PUBMED   23869016
  REMARK    GeneRIF: Mrap2 interacts directly with melanocortin 4 receptor
            (Mc4r), a protein previously implicated in mammalian obesity, and
            it enhances Mc4r-mediated generation of the second messenger cyclic
            adenosine monophosphate, suggesting that alterations in Mc4r
            signaling may be one mechanism underlying the association between
            Mrap2 disruption and obesity.
            Erratum:[Science. 2013 Aug 30;341(6149):959]
REFERENCE   10 (bases 1 to 2093)
  AUTHORS   Roy,S., Roy,S.J., Pinard,S., Agulleiro,M.J., Cerda-Reverter,J.M.,
            Parent,J.L. and Gallo-Payet,N.
  TITLE     The C-terminal domains of melanocortin-2 receptor (MC2R) accessory
            proteins (MRAP1) influence their localization and ACTH-induced cAMP
            production
  JOURNAL   Gen Comp Endocrinol 176 (2), 265-274 (2012)
   PUBMED   22366472
  REMARK    GeneRIF: The data suggest that MRAPalpha is involved in MC2R
            targeting to the plasma membrane, while MRAPbeta may enhance
            ACTH-MC2R coupling to cAMP production.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC168882.3, CJ059941.1, AC125370.4 and BQ175739.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 through 3 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219381.28772.1,
                                           SRR7345562.1076475.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-37                AC168882.3         198139-198175
            38-510              CJ059941.1         2-474
            511-1491            AC125370.4         86541-87521
            1492-2093           BQ175739.1         1-602               c
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 46.76 cM"
     gene            1..2093
                     /gene="Mrap2"
                     /note="melanocortin 2 receptor accessory protein 2"
                     /db_xref="GeneID:244958"
                     /db_xref="MGI:MGI:3609239"
     exon            1..92
                     /gene="Mrap2"
                     /inference="alignment:Splign:2.1.0"
     exon            93..237
                     /gene="Mrap2"
                     /inference="alignment:Splign:2.1.0"
     exon            238..371
                     /gene="Mrap2"
                     /inference="alignment:Splign:2.1.0"
     CDS             239..862
                     /gene="Mrap2"
                     /codon_start=1
                     /product="melanocortin-2 receptor accessory protein 2"
                     /protein_id="NP_001171202.1"
                     /db_xref="CCDS:CCDS52878.1"
                     /db_xref="GeneID:244958"
                     /db_xref="MGI:MGI:3609239"
                     /translation="MEMSAQRLASNRTSPQSPSNSDYTWEYEYYEIGPVSFEGLKAHK
                     YSIVIGFWVGLAVFVIFMFFVLTLLTKTGAPHQDNAESSERRFRMNSFVSDFGKPLES
                     DKVFSRQGNEESRSLFHCYINEVEHLDRVKVCHQTTAIDSDVHLQEASRSSGRPEEEL
                     ARFMKFDIPNFVNTEQSSFGEDDLLISEAPVLLENKPVSQTSRIDLD"
     exon            372..471
                     /gene="Mrap2"
                     /inference="alignment:Splign:2.1.0"
     exon            472..2076
                     /gene="Mrap2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1126..1131
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mrap2"
                     /note="hexamer: ATTAAA"
     polyA_site      1148
                     /gene="Mrap2"
                     /note="major polyA site"
ORIGIN      
        1 gttcactggc ggcggcggca cggcggcggt gaggctcgag caccggagga gctgaaccgt
       61 gtaaagcctg cggtaaccca ggacaggacg gggactccta agttcgaagc ctgctttgct
      121 gctgagatca ccccttgcgg cccaaggagt caggaaatcc tgcatatagc tccgcgggct
      181 gagctgagca tccagggggc gcagggcacc acgacccagc cacaccctct gccgcaggat
      241 ggagatgtct gcccagaggc tggcttctaa caggacatcc ccacagtcac catcgaactc
      301 tgactacacc tgggaatacg agtactatga gatcggacca gtttcctttg aaggactgaa
      361 ggctcataaa tattccattg tgattggatt ctgggttggt cttgcggtct ttgtgatttt
      421 catgtttttt gtgctgactt tgctgacgaa gacaggtgcc ccacaccaag acaacgcgga
      481 gtcctcagag aggaggttcc gcatgaacag ctttgtgtca gacttcggga agccgctgga
      541 gtcagacaag gtgttttctc gtcagggcaa cgaggagtcc aggtctctgt tccactgtta
      601 catcaatgaa gtagaacact tggacagggt taaagtttgc caccaaacaa cagccatcga
      661 cagtgatgtc cacctccagg aagccagcag aagcagtggg aggcctgagg aggagctagc
      721 caggttcatg aagtttgaca tccccaactt tgtgaacaca gagcagagct cctttgggga
      781 ggatgatctc ctgatttcgg aagcacccgt tcttctagaa aacaagccag tttcccagac
      841 atcacgcata gacctggact gagagacact tgtgccttac tgaggatgtg ttctgtctct
      901 gtacagcaag aaaccgcaag ctacccacac tcgtgtgtgt gtgtgtgtgt gtgtgtgtgt
      961 gtgtgtgtgt agagagagag agagagagag agagagagag agagagagag aggaggtgag
     1021 ggagggggag aggactaggc taggactggg atgtgataga ggggcagaat ttggagatgg
     1081 aaggcaccat tttggtttgg gggagatgat atttttcttt tttggattaa aaaaatgcat
     1141 tgacagtatt aaggcttctt tattgaaaca attagccaca tatatgcaat tgagccccct
     1201 cctgggtgag ggttaggtta ctgcagtctg atctagccct ttcccaccat tctctgcctg
     1261 taacaagttg ctttacctct ctggactgcc atggttttac atgtaaggtg gcctgtctgg
     1321 agtagatgac ctatcccagc tctagaattt ttgtggcttt cagacttagc gtttaagaca
     1381 tgctaagaaa cacataaaca gtagataata gaacatggca ccctggcttt cagggcaggt
     1441 tctccagggc ttggtaccct ccctttgctc agcccttcac agagtctcac tttagctagg
     1501 gaaaccttta ccaggagcac aggagcctca catgcactgt ggacagtgtc tctgcgagtt
     1561 ttgccgcagc agtttatgtg atgaactcca cccaccccgg ctttaaactg gggcagcttt
     1621 gactccagtg agtaccagcc taaactgttt cacaagcatc cgtgctctca aaaactgcta
     1681 gggcagattg tcatctgcct gatgtgggtc ccttctcggt gtctccgagt ttgcatcctg
     1741 tgctcgcgct tctgaactct ttggcttcag aacaagacgc tccttgccat ggtctgcgtg
     1801 gcagatgtgt gggtgtcggc tgcctggctt ggagctgaga gggtcagtgg gttgtttgtg
     1861 ccacagtagc agatgttcag gattgcgatg ataatttgag caacatgatg ttcatttcgt
     1921 ggaggaagca accctttgct ggtgtcggaa agaactcagc cttgcacaca gagagttgta
     1981 tgcatgagca gctgctaatc gatgggtggc atctacttta cgaatttgtt caccctttgt
     2041 cttgcttaag ttgcataata aatgcatttg atcgcaaaaa aaaaaaaaaa aaa
//
