LOCUS       NM_138670               1810 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus mercaptopyruvate sulfurtransferase (Mpst), transcript
            variant 1, mRNA.
ACCESSION   NM_138670
VERSION     NM_138670.4
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Drekolia,M.K., Karantanou,C., Wittig,I., Li,Y., Fuhrmann,D.C.,
            Brune,B., Katsouda,A., Hu,J., Papapetropoulos,A. and Bibli,S.I.
  TITLE     Loss of cardiac mitochondrial complex I persulfidation impairs NAD+
            homeostasis in aging
  JOURNAL   Redox Biol 69, 103014 (2024)
   PUBMED   38171255
REFERENCE   3  (bases 1 to 1810)
  AUTHORS   Katsouda,A., Markou,M., Zampas,P., Varela,A., Davos,C.H.,
            Vellecco,V., Cirino,G., Bucci,M. and Papapetropoulos,A.
  TITLE     CTH/MPST double ablation results in enhanced vasorelaxation and
            reduced blood pressure via upregulation of the eNOS/sGC pathway
  JOURNAL   Front Pharmacol 14, 1090654 (2023)
   PUBMED   36860295
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 1810)
  AUTHORS   Katsouda,A., Valakos,D., Dionellis,V.S., Bibli,S.I.,
            Akoumianakis,I., Karaliota,S., Zuhra,K., Fleming,I., Nagahara,N.,
            Havaki,S., Gorgoulis,V.G., Thanos,D., Antoniades,C., Szabo,C. and
            Papapetropoulos,A.
  TITLE     MPST sulfurtransferase maintains mitochondrial protein import and
            cellular bioenergetics to attenuate obesity
  JOURNAL   J Exp Med 219 (7) (2022)
   PUBMED   35616614
REFERENCE   5  (bases 1 to 1810)
  AUTHORS   Panagaki,T., Randi,E.B. and Szabo,C.
  TITLE     Role of 3-Mercaptopyruvate Sulfurtransferase in the Regulation of
            Proliferation and Cellular Bioenergetics in Human Down Syndrome
            Fibroblasts
  JOURNAL   Biomolecules 10 (4), 653 (2020)
   PUBMED   32340322
  REMARK    GeneRIF: Role of 3-Mercaptopyruvate Sulfurtransferase in the
            Regulation of Proliferation and Cellular Bioenergetics in Human
            Down Syndrome Fibroblasts.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1810)
  AUTHORS   Modis,K., Coletta,C., Erdelyi,K., Papapetropoulos,A. and Szabo,C.
  TITLE     Intramitochondrial hydrogen sulfide production by
            3-mercaptopyruvate sulfurtransferase maintains mitochondrial
            electron flow and supports cellular bioenergetics
  JOURNAL   FASEB J 27 (2), 601-611 (2013)
   PUBMED   23104984
  REMARK    GeneRIF: SiRNA silencing of 3-MPST reduced basal bioenergetic
            parameters and prevented the stimulating effect of 3-MP on
            mitochondrial bioenergetics.
REFERENCE   7  (bases 1 to 1810)
  AUTHORS   Nagahara,N., Nagano,M., Ito,T., Shimamura,K., Akimoto,T. and
            Suzuki,H.
  TITLE     Antioxidant enzyme, 3-mercaptopyruvate sulfurtransferase-knockout
            mice exhibit increased anxiety-like behaviors: a model for human
            mercaptolactate-cysteine disulfiduria
  JOURNAL   Sci Rep 3, 1986 (2013)
   PUBMED   23759691
  REMARK    GeneRIF: behavioral abnormality in MST-KO mice is caused by MST
            function defects such as an antioxidant insufficiency or a new
            transducer, H2S (or HS(-)) and/or SOx deficiency.
REFERENCE   8  (bases 1 to 1810)
  AUTHORS   Shibuya,N., Tanaka,M., Yoshida,M., Ogasawara,Y., Togawa,T.,
            Ishii,K. and Kimura,H.
  TITLE     3-Mercaptopyruvate sulfurtransferase produces hydrogen sulfide and
            bound sulfane sulfur in the brain
  JOURNAL   Antioxid Redox Signal 11 (4), 703-714 (2009)
   PUBMED   18855522
REFERENCE   9  (bases 1 to 1810)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   10 (bases 1 to 1810)
  AUTHORS   Da Cruz,S., Xenarios,I., Langridge,J., Vilbois,F., Parone,P.A. and
            Martinou,J.C.
  TITLE     Proteomic analysis of the mouse liver mitochondrial inner membrane
  JOURNAL   J Biol Chem 278 (42), 41566-41571 (2003)
   PUBMED   12865426
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL583893.17 and AL590144.10.
            
            On Jan 11, 2023 this sequence version replaced NM_138670.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1, 2 and 3 encode the same protein.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC094469.1, SRR7345562.143483.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-553               AL583893.17        177845-178397
            554-1175            AL583893.17        181170-181791
            1176-1228           AL583893.17        184506-184558
            1229-1810           AL590144.10        164096-164677       c
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 37.47 cM"
     gene            1..1810
                     /gene="Mpst"
                     /gene_synonym="3MST; Mst"
                     /note="mercaptopyruvate sulfurtransferase"
                     /db_xref="GeneID:246221"
                     /db_xref="MGI:MGI:2179733"
     exon            1..553
                     /gene="Mpst"
                     /gene_synonym="3MST; Mst"
                     /inference="alignment:Splign:2.1.0"
     exon            554..1175
                     /gene="Mpst"
                     /gene_synonym="3MST; Mst"
                     /inference="alignment:Splign:2.1.0"
     CDS             581..1474
                     /gene="Mpst"
                     /gene_synonym="3MST; Mst"
                     /EC_number="2.8.1.2"
                     /codon_start=1
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="NP_619611.3"
                     /db_xref="CCDS:CCDS27615.1"
                     /db_xref="GeneID:246221"
                     /db_xref="MGI:MGI:2179733"
                     /translation="MAAPQLFRALVSAQWVAEALKAPRSSQPLKLLDASWYLPKLGRD
                     ARREFEERHIPGAAFFDIDRCSDHTSPYDHMLPNATHFADYAGSLGVSAATHVVIYDD
                     SDQGLYSAPRVWWMFRAFGHHSVSLLDGGFRHWLNQNLPISSGKSHSEPAEFSAQLDP
                     SFIKTHEDILENLDARRFQVVDARAAGRFQGTQPEPRDGIEPGHIPGSVNIPFTEFLT
                     NEGLEKSPEEIKRLFKEKKVDLSKPLVATCGSGVTACHVVLGAFLCGKSDVPVYDGSW
                     VEWYMRAQPEHIISEGRGKTQ"
     exon            1176..1810
                     /gene="Mpst"
                     /gene_synonym="3MST; Mst"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1789..1794
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mpst"
                     /gene_synonym="3MST; Mst"
                     /note="hexamer: AATAAA"
     polyA_site      1810
                     /gene="Mpst"
                     /gene_synonym="3MST; Mst"
                     /note="major polyA site"
ORIGIN      
        1 aagtctcctc cctttggctg gccgcaggtt ggtggcggaa ggagggaacc tctgagggcg
       61 tggggagggg gtgacatggc ggagctggga aacggggagc gcaaatccct ggtatctgct
      121 acccaacgcg tcctacttgc ttttctgtga gagagggtgc ttctccgggg gtgcatagcg
      181 tggtatcacg cgggagtgga ggaggggatg cagtctatga gagaagagtt tccagggcgc
      241 ctctaatttg ccttaggaag actgtccgtg gctgcttgtg gccgtctgtg ggttggtgtg
      301 ggataagaga caggtgggta gcagtgagtg gatggggttt ggaaagacct gatgcctcct
      361 gagaaagttg ggggggggga gcaagagaat aaacaaagca attcagagga gctgtggtga
      421 ggagatctag catgccccaa gaggagaaag taacagaacg gaataaaaat aaaactagca
      481 tttatgaagc ccgccttgcc gagtgccttc acgaccctgc cggattgagc tgaactccca
      541 acaggagaaa cagccccgca atctgagcat ctcctaggcc atggccgccc cgcaactttt
      601 ccgagctctg gtgtctgcgc agtgggtggc ggaggctctg aaggccccgc gctcctcaca
      661 gccgctgaag ttactggacg cgtcctggta cctacccaag ctgggccgcg atgcacgacg
      721 cgagtttgag gagcgccaca tcccgggcgc cgccttcttc gacatcgatc gttgcagtga
      781 ccacacgtcc ccctacgacc acatgctgcc taacgccacg cactttgccg actacgcggg
      841 cagcttgggc gtgagcgccg ccacgcacgt cgtgatctac gacgacagtg accagggcct
      901 ctattccgct ccgcgggtct ggtggatgtt ccgcgccttc ggccaccact ctgtgtcatt
      961 gctggacggc ggcttccgcc actggctgaa tcagaacctg ccaatcagct ccggcaaaag
     1021 ccactcggag cccgcggagt tcagcgcgca gctcgatccc tctttcatca agacccacga
     1081 ggacatcctg gagaacttgg atgcccggcg cttccaggtg gtggacgccc gcgcagctgg
     1141 ccgtttccaa ggcacccagc cagaaccccg agatggcatc gaacctggcc acatccctgg
     1201 ctcagtaaac atcccgttca ctgaattcct gaccaacgaa ggcctggaga agagcccaga
     1261 ggagatcaaa cgcctgttta aggagaagaa ggtggacctg tctaagccct tggtagccac
     1321 gtgtggctcc ggtgtcacag cctgccacgt ggtcctgggg gccttcctct gtggcaagtc
     1381 agacgtgcct gtctatgatg gctcctgggt agagtggtac atgcgtgccc agccagagca
     1441 tatcatctct gagggccgag ggaagaccca gtgaagccag gcaggacaca gtgcagcttg
     1501 ggtgacatct gaccatcaca gtaccagcca ctggtcactc tgaccctgct ttgccaagag
     1561 tcctttctca gacaactgag ggagtgtttg gggtgatatc ccagagacct ggagttccag
     1621 tgactcatag atactcagct gaggtagcca agaaggtggc aggcagtaag agggtaccca
     1681 ggggagggag ggagggaggg aggaagggag gaagggaggg aggccagata gcaaaacagc
     1741 ttgcacggtg ctaagctggg gcccacctag cttgtttcat tgttgggaaa taaaacatca
     1801 agaacaaaaa
//
