LOCUS       NM_001191012            2382 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus megakaryocyte and platelet inhibitory receptor G6b
            (Mpig6b), transcript variant 1, mRNA.
ACCESSION   NM_001191012
VERSION     NM_001191012.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Vogtle,T., Sharma,S., Mori,J., Nagy,Z., Semeniak,D., Scandola,C.,
            Geer,M.J., Smith,C.W., Lane,J., Pollack,S., Lassila,R.,
            Jouppila,A., Barr,A.J., Ogg,D.J., Howard,T.D., McMiken,H.J.,
            Warwicker,J., Geh,C., Rowlinson,R., Abbott,W.M., Eckly,A.,
            Schulze,H., Wright,G.J., Mazharian,A., Futterer,K., Rajesh,S.,
            Douglas,M.R. and Senis,Y.A.
  TITLE     Heparan sulfates are critical regulators of the inhibitory
            megakaryocyte-platelet receptor G6b-B
  JOURNAL   Elife 8, e46840 (2019)
   PUBMED   31436532
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2382)
  AUTHORS   Geer,M.J., van Geffen,J.P., Gopalasingam,P., Vogtle,T., Smith,C.W.,
            Heising,S., Kuijpers,M.J.E., Tullemans,B.M.E., Jarvis,G.E.,
            Eble,J.A., Jeeves,M., Overduin,M., Heemskerk,J.W.M., Mazharian,A.
            and Senis,Y.A.
  TITLE     Uncoupling ITIM receptor G6b-B from tyrosine phosphatases Shp1 and
            Shp2 disrupts murine platelet homeostasis
  JOURNAL   Blood 132 (13), 1413-1425 (2018)
   PUBMED   29891536
  REMARK    GeneRIF: Uncoupling of G6b-B from Shp1 and Shp2 results in severe
            macrothrombocytopenia and aberrant platelet function.
REFERENCE   4  (bases 1 to 2382)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   5  (bases 1 to 2382)
  AUTHORS   West,D.B., Engelhard,E.K., Adkisson,M., Nava,A.J., Kirov,J.V.,
            Cipollone,A., Willis,B., Rapp,J., de Jong,P.J. and Lloyd,K.C.
  TITLE     Transcriptome Analysis of Targeted Mouse Mutations Reveals the
            Topography of Local Changes in Gene Expression
  JOURNAL   PLoS Genet 12 (2), e1005691 (2016)
   PUBMED   26839965
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2382)
  AUTHORS   Mazharian,A., Mori,J., Wang,Y.J., Heising,S., Neel,B.G.,
            Watson,S.P. and Senis,Y.A.
  TITLE     Megakaryocyte-specific deletion of the protein-tyrosine
            phosphatases Shp1 and Shp2 causes abnormal megakaryocyte
            development, platelet production, and function
  JOURNAL   Blood 121 (20), 4205-4220 (2013)
   PUBMED   23509158
REFERENCE   7  (bases 1 to 2382)
  AUTHORS   Mazharian,A., Wang,Y.J., Mori,J., Bem,D., Finney,B., Heising,S.,
            Gissen,P., White,J.G., Berndt,M.C., Gardiner,E.E., Nieswandt,B.,
            Douglas,M.R., Campbell,R.D., Watson,S.P. and Senis,Y.A.
  TITLE     Mice lacking the ITIM-containing receptor G6b-B exhibit
            macrothrombocytopenia and aberrant platelet function
  JOURNAL   Sci Signal 5 (248), ra78 (2012)
   PUBMED   23112346
  REMARK    GeneRIF: these findings establish G6b-B as a major inhibitory
            receptor regulating megakaryocyte activation, function, and
            platelet production.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK137516.1 and GU269270.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: GU269270.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849387
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                AK137516.1         2-15
            15-743              GU269270.1         1-729
            744-886             AK137516.1         615-757
            887-2382            AK137516.1         759-2254
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 18.59 cM"
     gene            1..2382
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /note="megakaryocyte and platelet inhibitory receptor G6b"
                     /db_xref="GeneID:106722"
                     /db_xref="MGI:MGI:2146995"
     exon            1..75
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..743
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; ITIM-receptor G6b-B; immunoreceptor
                     tyrosine-based inhibitory motif (ITIM) containing platelet
                     receptor; protein G6b"
                     /codon_start=1
                     /product="megakaryocyte and platelet inhibitory receptor
                     G6b isoform 1 precursor"
                     /protein_id="NP_001177941.1"
                     /db_xref="CCDS:CCDS57071.1"
                     /db_xref="GeneID:106722"
                     /db_xref="MGI:MGI:2146995"
                     /translation="MALVLPLLPLLLSKVQGNPEVSLEGSPGDRVNLSCIGVSDPTRW
                     AWAPSFPACKGLSKGRRPILWASTRGTPTVLQHFSGRLRSLDNGIKRLELLLSAGDSG
                     TFFCKGRHENESRTVLQVLGDKAGCRPAGSTHGYEYPKVLIPLLGVGLVLGLGVAGVV
                     WRRRRLSPPPPPPPPPGPLPTFAPVINAEPQRPLEQESKISGHLDQEPSLHYADLDHS
                     VLGRHRRMSTVVSGDASTVYAVVV"
     exon            76..417
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /inference="alignment:Splign:2.1.0"
     exon            418..508
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /inference="alignment:Splign:2.1.0"
     exon            509..561
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /inference="alignment:Splign:2.1.0"
     exon            562..638
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /inference="alignment:Splign:2.1.0"
     exon            639..2382
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2057..2062
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /note="hexamer: AATAAA"
     polyA_site      2076
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /note="major polyA site"
     regulatory      2244..2249
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
                     /note="hexamer: AATAAA"
     polyA_site      2265
                     /gene="Mpig6b"
                     /gene_synonym="C6orf25; G6b; Ng31"
ORIGIN      
        1 accacatccc aaccatggcc ttggtcctgc cgctgctgcc tttgttgctc tcaaaggtcc
       61 aggggaatcc cgaggtttct ttggagggca gccctgggga ccgggtgaat ctctcctgca
      121 taggggtctc cgaccccacc cgctgggctt gggcgcctag tttcccagca tgcaagggct
      181 tgtctaaagg gcgccgtccg atcttgtggg cctcgacgag agggacccca actgtgctcc
      241 agcatttctc tggccgcctg cgttccctgg acaatggtat caagcggcta gagctgctgc
      301 tgagcgccgg ggattctgga acctttttct gcaaaggacg ccacgagaat gagagtcgca
      361 cagtgcttca agtgttaggg gacaaggcag gttgccggcc tgcaggatct acccacgggt
      421 acgagtatcc caaggtcctg attccgctcc tgggcgttgg gcttgtgctg ggactgggag
      481 tcgcgggcgt ggtctggcgg cggcgcagac tctcgcctcc gcccccaccc ccgcccccgc
      541 ctggaccact acccacattt gctccagtca taaatgctga gccacagagg cctttagaac
      601 aggagtccaa gatctcaggc cacctggacc aggagccgag cctgcactac gctgatctgg
      661 accactctgt cctcgggagg caccgccgga tgtccacagt ggtttctggt gatgcctcca
      721 ctgtctatgc ggttgtagta taaagggaag cccttgttct gccaaccctt tctatctggg
      781 gctcccctgt ataacctcag ctagaagggg caggcaccat ggacagaggc actgatagag
      841 gcgactgact acctggtttg cgttctgcca atggtcaact ttttttctgt cacttccatc
      901 ttctgtaaca tctttccttc tccactgctg ccctctctaa agatgatcaa gctgcctgtt
      961 aagctttttc ttcgcgcccc cccccaagac tgggatttct ctgtatagcc ttggctgtct
     1021 tggaactccg tagaccatag accaagctgg ccttgaactc agagaattca tctgcttctg
     1081 cctcccaact tctgagttta aaggcactgc ctggcccaat gaagcatttc aaactttaga
     1141 catagacctg cctcgttacc ggtctgactg cactccccct gctcattggc tgctgctgct
     1201 gctcatccac tgaaaggttg ccagtgatgt cctaccacca gataacattc acccctgttt
     1261 aaaccagggt ttactttgtt gctattgtct ttgtagtagc actgttctgc tcatctcttt
     1321 gtttctcctc cgtactcttc taggaagctg gccaatcaat cttcagcccc caaatgatac
     1381 acagtagtcc ttccaagcca gatctcagac ctagttcact ctgtccagat ggcctcaggc
     1441 attttgccca aggcttgtca aacctgactc agtcatttct taagccatcc cctgggtcct
     1501 cctctgaaag ctcttgaaga acccaagttg tccaagccag aaactggggt cagagtacac
     1561 catctcctct cccacacaag gacttatcag gaccctaacc cgccgcttcc actcctttta
     1621 aagacaggct ctcttatggc tgaggctgac tttgaaatct ccctctcctt atgtctacct
     1681 tcagtgtttg gataaaagtg attgcaagca tgcaccacca accacacaag gccttgtctg
     1741 tagcaggtca ctgtcctctg tacctttctc catgtccaat gctgccacca gaatgtactg
     1801 tcatcatttg cttcatgatg acccacagac ccctctgatc ctcgtgccct gctcttccta
     1861 ctcaaagcca gcaagattcc caggatcaac cactggttct ttcccaaggt ttcagctctg
     1921 gggctgtgag cgtgggctct gtgttcaggc tccagcactg ggcccctttt taggcctatc
     1981 catcattttc tgtgtgcaca cgccctcaga gagtttctcc acaatgttct ccctacaata
     2041 ctcgttggct ctttgaaata aattctgttt tctggattcc tttttcctct tcctcccttc
     2101 ccacctggat aattgccccc caggatttca ttcaaatgta gctttttctg agatagcctc
     2161 atcccaaatg cttgcctact tctgcacaaa atttgatgct ctagaaaccc cctggttttt
     2221 ccttatagaa ccagactcca gcaaataaat caccccatta caacacgtct gtcagtttat
     2281 aagaaaatat caggaagtat gagttcagcc ttggaccagg aatgagcgtc ctggaaaaca
     2341 tccctttctt ctttatcaaa taaaggcaga ctttgaatcc tg
//
