LOCUS       NM_001374668            4222 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus macrophage expressed gene 1 (Mpeg1), transcript
            variant 2, mRNA.
ACCESSION   NM_001374668
VERSION     NM_001374668.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Rodriguez-Silvestre,P., Laub,M., Krawczyk,P.A., Davies,A.K.,
            Schessner,J.P., Parveen,R., Tuck,B.J., McEwan,W.A., Borner,G.H.H.
            and Kozik,P.
  TITLE     Perforin-2 is a pore-forming effector of endocytic escape in
            cross-presenting dendritic cells
  JOURNAL   Science 380 (6651), 1258-1265 (2023)
   PUBMED   37347855
  REMARK    GeneRIF: Perforin-2 is a pore-forming effector of endocytic escape
            in cross-presenting dendritic cells.
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Yu,X., Ni,T., Munson,G., Zhang,P. and Gilbert,R.J.C.
  TITLE     Cryo-EM structures of perforin-2 in isolation and assembled on a
            membrane suggest a mechanism for pore formation
  JOURNAL   EMBO J 41 (23), e111857 (2022)
   PUBMED   36245269
REFERENCE   3  (bases 1 to 4222)
  AUTHORS   Ebrahimnezhaddarzi,S., Bird,C.H., Allison,C.C., Tuipulotu,D.E.,
            Kostoulias,X., Macri,C., Stutz,M.D., Abraham,G., Kaiserman,D.,
            Pang,S.S., Man,S.M., Mintern,J.D., Naderer,T., Peleg,A.Y.,
            Pellegrini,M., Whisstock,J.C. and Bird,P.I.
  TITLE     Mpeg1 is not essential for antibacterial or antiviral immunity, but
            is implicated in antigen presentation
  JOURNAL   Immunol Cell Biol 100 (7), 529-546 (2022)
   PUBMED   35471730
  REMARK    GeneRIF: Mpeg1 is not essential for antibacterial or antiviral
            immunity, but is implicated in antigen presentation.
REFERENCE   4  (bases 1 to 4222)
  AUTHORS   Pastar,I., Sawaya,A.P., Marjanovic,J., Burgess,J.L., Strbo,N.,
            Rivas,K.E., Wikramanayake,T.C., Head,C.R., Stone,R.C., Jozic,I.,
            Stojadinovic,O., Kornfeld,E.Y., Kirsner,R.S., Lev-Tov,H. and
            Tomic-Canic,M.
  TITLE     Intracellular Staphylococcus aureus triggers pyroptosis and
            contributes to inhibition of healing due to perforin-2 suppression
  JOURNAL   J Clin Invest 131 (24) (2021)
   PUBMED   34730110
  REMARK    GeneRIF: Intracellular Staphylococcus aureus triggers pyroptosis
            and contributes to inhibition of healing due to perforin-2
            suppression.
REFERENCE   5  (bases 1 to 4222)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4222)
  AUTHORS   Oeschger,F.M., Wang,W.Z., Lee,S., Garcia-Moreno,F., Goffinet,A.M.,
            Arbones,M.L., Rakic,S. and Molnar,Z.
  TITLE     Gene expression analysis of the embryonic subplate
  JOURNAL   Cereb Cortex 22 (6), 1343-1359 (2012)
   PUBMED   21862448
REFERENCE   7  (bases 1 to 4222)
  AUTHORS   Abdallah,B.M., Ditzel,N., Mahmood,A., Isa,A., Traustadottir,G.A.,
            Schilling,A.F., Ruiz-Hidalgo,M.J., Laborda,J., Amling,M. and
            Kassem,M.
  TITLE     DLK1 is a novel regulator of bone mass that mediates estrogen
            deficiency-induced bone loss in mice
  JOURNAL   J Bone Miner Res 26 (7), 1457-1471 (2011)
   PUBMED   21308776
REFERENCE   8  (bases 1 to 4222)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   9  (bases 1 to 4222)
  AUTHORS   Wada,R., Tifft,C.J. and Proia,R.L.
  TITLE     Microglial activation precedes acute neurodegeneration in Sandhoff
            disease and is suppressed by bone marrow transplantation
  JOURNAL   Proc Natl Acad Sci U S A 97 (20), 10954-10959 (2000)
   PUBMED   11005868
REFERENCE   10 (bases 1 to 4222)
  AUTHORS   Spilsbury,K., O'Mara,M.A., Wu,W.M., Rowe,P.B., Symonds,G. and
            Takayama,Y.
  TITLE     Isolation of a novel macrophage-specific gene by differential cDNA
            analysis
  JOURNAL   Blood 85 (6), 1620-1629 (1995)
   PUBMED   7888681
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151730.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13422597.755442.1,
                                           SRR10662772.3838782.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-92                AC151730.5         115103-115194       c
            93-4222             AC151730.5         110684-114813       c
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="19"
                     /map="19 8.72 cM"
     gene            1..4222
                     /gene="Mpeg1"
                     /gene_synonym="mPFN2; Mpg-1; MPS1; P-2; PFN2"
                     /note="macrophage expressed gene 1"
                     /db_xref="GeneID:17476"
                     /db_xref="MGI:MGI:1333743"
     exon            1..92
                     /gene="Mpeg1"
                     /gene_synonym="mPFN2; Mpg-1; MPS1; P-2; PFN2"
                     /inference="alignment:Splign:2.1.0"
     exon            93..4222
                     /gene="Mpeg1"
                     /gene_synonym="mPFN2; Mpg-1; MPS1; P-2; PFN2"
                     /inference="alignment:Splign:2.1.0"
     CDS             143..2284
                     /gene="Mpeg1"
                     /gene_synonym="mPFN2; Mpg-1; MPS1; P-2; PFN2"
                     /note="macrophage gene 1 protein; macrophage-expressed
                     gene 1 protein; perforin-2"
                     /codon_start=1
                     /product="macrophage-expressed gene 1 protein precursor"
                     /protein_id="NP_001361597.1"
                     /db_xref="GeneID:17476"
                     /db_xref="MGI:MGI:1333743"
                     /translation="MNSFMALVLIWMIIACAEADKPLGETGTTGFQICKNALKLPVLE
                     VLPGGGWDNLRNVDMGRVMDLTYTNCKTTEDGQYIIPDEVYTIPQKESNLEMNSEVLE
                     SWMNYQSTTSLSINTELALFSRVNGKFSTEFQRMKTLQVKDQAVTTRVQVRNRIYTVK
                     TTPTSELSLGFTKALMDICDQLEKNQTKMATYLAELLILNYGTHVITSVDAGAALVQE
                     DHVRSSFLLDNQNSQNTVTASAGIAFLNIVNFKVETDYISQTSLTKDYLSNRTNSRVQ
                     SFGGVPFYPGITLETWQKGITNHLVAIDRAGLPLHFFIKPDKLPGLPGPLVKKLSKTV
                     ETAVRHYYTFNTHPGCTNVDSPNFNFQANMDDDSCDAKVTNFTFGGVYQECTELSGDV
                     LCQNLEQKNLLTGDFSCPPGYTPVHLLSQTHEEGYSRLECKKKCTLKIFCKTVCEDVF
                     RVAKAEFRAYWCVAAGQVPDNSGLLFGGVFTDKTINPMTNAQSCPAGYIPLNLFESLK
                     VCVSLDYELGFKFSVPFGGFFSCIMGNPLVNSDTAKDVRAPSLKKCPGGFSQHLAVIS
                     DGCQVSYCVKAGIFTGGSLLPVRLPPYTKPPLMSQVATNTVIVTNSETARSWIKDPQT
                     NQWKLGEPLELRRAMTVIHGDSNGMSGGEAAGITLGVTIALGVVITLAIYGTRKYKKK
                     EYQEIEEQESLVGSLATDATVLNGEEDPSPA"
     regulatory      4200..4205
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mpeg1"
                     /gene_synonym="mPFN2; Mpg-1; MPS1; P-2; PFN2"
                     /note="hexamer: ATTAAA"
     polyA_site      4222
                     /gene="Mpeg1"
                     /gene_synonym="mPFN2; Mpg-1; MPS1; P-2; PFN2"
                     /note="major polyA site"
ORIGIN      
        1 aaactaatac tgtgatttca caaaagaaga gaaaggcagg ttttgaagtc aaagagagtg
       61 aaacaaaagc cagacagagc cttctgacag agtcttgttg gtgaatgctt agggctggta
      121 tatggccaaa accatctgcg ccatgaacag cttcatggcc ttggtcctca tctggatgat
      181 aatagcgtgt gctgaagcag acaagcctct tggagaaacg ggtaccactg gatttcaaat
      241 atgcaagaat gccctgaaac tacctgtctt ggaggtccta ccaggaggag gctgggataa
      301 tctgagaaat gtagacatgg gacgggtgat ggacttgaca tacaccaact gtaagaccac
      361 agaagatggg cagtacatca tccccgatga agtgtatact attcctcaga aagagagcaa
      421 cctggagatg aactcagaag tcctggagtc ctggatgaat taccagagta ccacctcact
      481 ttctatcaac acagaactcg cccttttctc cagagtcaac ggcaagttct ctactgagtt
      541 ccaaaggatg aagacccttc aagtaaagga ccaagctgtg actaccaggg ttcaggtaag
      601 aaaccggatc tacacagtga aaaccacccc aacttcagag ctcagcttgg ggtttacgaa
      661 ggcacttatg gacatctgtg accaactaga gaaaaaccag acgaagatgg ccacctacct
      721 ggcagagctc ttgatcctca actatggcac acacgtaatc actagtgtgg atgctggggc
      781 tgcactggtt caggaggatc acgtaaggtc ctccttcctt ctggacaacc agaatagcca
      841 gaacaccgtg accgcttctg cagggattgc cttcttaaac attgtgaact tcaaagttga
      901 aacagactac atttctcaga ccagtttgac gaaggactac ctgtcgaaca ggaccaactc
      961 cagggtgcag agttttggag gggttccctt ctatccaggc atcaccttag aaacctggca
     1021 gaagggcatc actaaccacc tagtggcaat agaccgtgct ggcttgcctc tgcatttctt
     1081 cattaaacct gacaagctac ctggcttgcc aggtcccttg gtgaagaagc tgtcgaagac
     1141 agtggaaact gctgtgagac actattacac ttttaacact cacccaggat gcacaaatgt
     1201 tgattccccc aactttaatt ttcaagccaa tatggatgat gattcctgtg atgcgaaagt
     1261 caccaacttc acctttggtg gagtttatca ggaatgcact gaactgtcag gtgatgttct
     1321 ttgccaaaac ctggagcaga agaacctgct cacaggtgat ttctcttgtc cccctggcta
     1381 cacccctgtc catctgctct cccagaccca tgaagagggt tacagtcgtc tggaatgtaa
     1441 aaagaaatgc accctcaaga ttttctgcaa gacagtgtgt gaagatgtgt tcagagtggc
     1501 caaggctgaa tttagggctt attggtgtgt ggctgctggc caagtacctg acaactcagg
     1561 acttctcttt ggaggagtct tcactgacaa gaccatcaac cctatgacaa atgcacagtc
     1621 atgcccagca ggctacatcc cactgaacct gtttgaaagc ctcaaggtat gtgtgtccct
     1681 ggattatgag ttggggttca agttttcagt cccctttggt gggttcttca gttgtataat
     1741 ggggaacccc ttggttaatt ctgatacagc taaagacgtc agagcaccat ctctgaaaaa
     1801 gtgtcccggg ggcttcagcc aacacctagc tgttatcagt gatggatgcc aagtgtccta
     1861 ctgtgtcaag gctggaatct tcacaggagg gtccctgctc cctgtcaggc tcccacctta
     1921 taccaaacca cctcttatga gccaggttgc caccaacact gtcatagtga ccaatagtga
     1981 gactgccaga tcctggatta aggatcctca gaccaaccag tggaagctgg gagaacctct
     2041 ggagcttcgt agggccatga cagtcatcca tggggacagt aatggaatgt caggagggga
     2101 agctgctgga atcactttgg gagtcaccat agcactagga gttgtcatta ccttggccat
     2161 ctatggtact cggaagtaca agaagaagga ataccaggaa attgaggagc aggagagttt
     2221 ggttggaagc ttagcaacag atgcaacagt ccttaatgga gaagaggatc caagtccagc
     2281 ttaattgtct ccaaaggaaa cagtttccag ccacagcttc aagcacatct tttgctttgt
     2341 tttctctact tctgccttcc taagtgactg aagtgacagt cgccatagga agaaagcagc
     2401 tattacaacc ctggcagtat tttcttaggc ctctgcacca ggaaattaaa ggagccattt
     2461 gaagtgaggt gtggggaggg gtatgattca tttggaaaaa gctggactgg gactaagcct
     2521 cggggtgtat gcgtccattc tcatagttgt ctgtacctga atgcatgtgt gtggaacgag
     2581 gggtggggga gaaattggat ttggtgcatt tagtctgaat ttgaaaggct ttccagtttg
     2641 taagctgaag aggtctgtgc tttaagtaca ttacttcttt tcttgtgttt tcaaagaggc
     2701 cctcagggtt tcagtaaaca gtgccctgaa atgccacagg aggcctacat gccccctact
     2761 ctttagcttt aaggacttgg tgttgattga cagtgccctc cccatgagtc acctcacagg
     2821 catttgaagg agaagcgggt ggggtttata caaattccat ttcccaactt cctcacagct
     2881 aatctaggtc agtcttgggc ttctgaactg tgcaaacaag tcctgggggg ggggggggga
     2941 atataacata attttctgtt aggggatgaa ggatgaagaa acagtaagga aatgagtaat
     3001 aaattaaggc taaaaaccgg ggttcgggag acaatgagag tattctagga aactttgaat
     3061 tcttagatct gtcaattaac agggttataa atgaagaact aaacagagct ctagtttggc
     3121 agatcaagca gtatggtttc acgttcatgt gtcaaaaatg taactccttc ctctctctgg
     3181 acagtctttc cccttgtctt cttaccacag ctgagggtgc acaggaatac acacaaaccc
     3241 cttggactgt agcaagaagc ttttatctgc ctaggggtga aaaatgcgca agtcacagtg
     3301 agcctgcact tacatagtac ttcctcccta aaactaaagc tattgggaaa gcgctgtctt
     3361 ctgtctgtta ggatttgcct tttcgcgctg cttggaagag gggtggggta atttaaaaca
     3421 aaacaaaatt tatgtcacag ttagctggga aaaggccatg atatgtattt cagttccagg
     3481 atggcttcca tgggttatct ggaaggcctc gtgattgaca actggcaata gttggggtat
     3541 tgctcaattt tcatatctga gaatttaatt ctctacaaca cggaaattcc tctacagctg
     3601 tcttagaaat tatgtatcta ttcaccacaa cacatcagtg agctatcttc tacgaaagat
     3661 gatgtattct tgctttaaca cttacaatca agctcttgtg cacagagtag gccctcctga
     3721 agagctccaa gtgtctcagc cttcaggaac agggagagac ttcctcattc gtttccattt
     3781 gagatacaga gaggaatgaa tgactgaatt ggaattctgc cctaccacct gcccagccaa
     3841 gagggctaat tgcacaacct ctccatcgaa cctgcctctt gagcgttaaa atgttcttct
     3901 taacctctgt gtgctttgtg ggtttgggct atattttaat gtttcattga agccagccat
     3961 ctttctgaaa atactagtta caatcaagct cttgtgcaca gagtaggccc tcctgaagag
     4021 ctccaagtgt ctcagccttc aggaacgggg agagacttct ttctgtaaat tctctgaatc
     4081 tgcaaagcaa ggcacatcag aagactccca gatctttaat gcagaaactt cctgttgaat
     4141 tttttaatat ttgtcccata aacatgtaac tctactaaca aaaatgagta taaatatgca
     4201 ttaaaatgtg tacttggcaa aa
//
