LOCUS       NM_027430                806 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus mitochondrial pyruvate carrier 2 (Mpc2), mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_027430
VERSION     NM_027430.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Chen,C., Zheng,H., Horwitz,E.M., Ando,S., Araki,K., Zhao,P., Li,Z.,
            Ford,M.L., Ahmed,R. and Qu,C.K.
  TITLE     Mitochondrial metabolic flexibility is critical for CD8+ T cell
            antitumor immunity
  JOURNAL   Sci Adv 9 (49), eadf9522 (2023)
   PUBMED   38055827
REFERENCE   2  (bases 1 to 806)
  AUTHORS   Yiew,N.K.H., Vazquez,J.H., Martino,M.R., Kennon-McGill,S.,
            Price,J.R., Allard,F.D., Yee,E.U., Layman,A.J., James,L.P.,
            McCommis,K.S., Finck,B.N. and McGill,M.R.
  TITLE     Hepatic pyruvate and alanine metabolism are critical and
            complementary for maintenance of antioxidant capacity and
            resistance to oxidative insult
  JOURNAL   Mol Metab 77, 101808 (2023)
   PUBMED   37716594
REFERENCE   3  (bases 1 to 806)
  AUTHORS   Yiew,N.K.H., Deja,S., Ferguson,D., Cho,K., Jarasvaraparn,C.,
            Jacome-Sosa,M., Lutkewitte,A.J., Mukherjee,S., Fu,X., Singer,J.M.,
            Patti,G.J., Burgess,S.C. and Finck,B.N.
  TITLE     Effects of hepatic mitochondrial pyruvate carrier deficiency on de
            novo lipogenesis and gluconeogenesis in mice
  JOURNAL   iScience 26 (11), 108196 (2023)
   PUBMED   37942005
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 806)
  AUTHORS   Zhu,B., Wei,X., Narasimhan,H., Qian,W., Zhang,R., Cheon,I.S.,
            Wu,Y., Li,C., Jones,R.G., Kaplan,M.H., Vassallo,R.A.,
            Braciale,T.J., Somerville,L., Colca,J.R., Pandey,A.,
            Jackson,P.E.H., Mann,B.J., Krawczyk,C.M., Sturek,J.M. and Sun,J.
  TITLE     Inhibition of the mitochondrial pyruvate carrier simultaneously
            mitigates hyperinflammation and hyperglycemia in COVID-19
  JOURNAL   Sci Immunol 8 (82), eadf0348 (2023)
   PUBMED   36821695
REFERENCE   5  (bases 1 to 806)
  AUTHORS   Hodges,W.T., Jarasvaraparn,C., Ferguson,D., Griffett,K., Gill,L.E.,
            Chen,Y., Ilagan,M.X.G., Hegazy,L., Elgendy,B., Cho,K., Patti,G.J.,
            McCommis,K.S. and Finck,B.N.
  TITLE     Mitochondrial pyruvate carrier inhibitors improve metabolic
            parameters in diet-induced obese mice
  JOURNAL   J Biol Chem 298 (2), 101554 (2022)
   PUBMED   34973337
REFERENCE   6  (bases 1 to 806)
  AUTHORS   Bricker,D.K., Taylor,E.B., Schell,J.C., Orsak,T., Boutron,A.,
            Chen,Y.C., Cox,J.E., Cardon,C.M., Van Vranken,J.G., Dephoure,N.,
            Redin,C., Boudina,S., Gygi,S.P., Brivet,M., Thummel,C.S. and
            Rutter,J.
  TITLE     A mitochondrial pyruvate carrier required for pyruvate uptake in
            yeast, Drosophila, and humans
  JOURNAL   Science 337 (6090), 96-100 (2012)
   PUBMED   22628558
REFERENCE   7  (bases 1 to 806)
  AUTHORS   Herzig,S., Raemy,E., Montessuit,S., Veuthey,J.L., Zamboni,N.,
            Westermann,B., Kunji,E.R. and Martinou,J.C.
  TITLE     Identification and functional expression of the mitochondrial
            pyruvate carrier
  JOURNAL   Science 337 (6090), 93-96 (2012)
   PUBMED   22628554
REFERENCE   8  (bases 1 to 806)
  AUTHORS   Kim,S.C., Sprung,R., Chen,Y., Xu,Y., Ball,H., Pei,J., Cheng,T.,
            Kho,Y., Xiao,H., Xiao,L., Grishin,N.V., White,M., Yang,X.J. and
            Zhao,Y.
  TITLE     Substrate and functional diversity of lysine acetylation revealed
            by a proteomics survey
  JOURNAL   Mol Cell 23 (4), 607-618 (2006)
   PUBMED   16916647
REFERENCE   9  (bases 1 to 806)
  AUTHORS   Mootha,V.K., Bunkenborg,J., Olsen,J.V., Hjerrild,M.,
            Wisniewski,J.R., Stahl,E., Bolouri,M.S., Ray,H.N., Sihag,S.,
            Kamal,M., Patterson,N., Lander,E.S. and Mann,M.
  TITLE     Integrated analysis of protein composition, tissue diversity, and
            gene regulation in mouse mitochondria
  JOURNAL   Cell 115 (5), 629-640 (2003)
   PUBMED   14651853
REFERENCE   10 (bases 1 to 806)
  AUTHORS   Eddleston,J., Murdoch,J.N., Copp,A.J. and Stanier,P.
  TITLE     Physical and transcriptional map of a 3-Mb region of mouse
            chromosome 1 containing the gene for the neural tube defect mutant
            loop-tail (Lp)
  JOURNAL   Genomics 56 (2), 149-159 (1999)
   PUBMED   10051400
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162789.4.
            
            On Jan 26, 2019 this sequence version replaced NM_027430.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK002233.2, BU525971.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            RefSeq Select criteria             :: based on single
                                                  protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-252               AC162789.4         138395-138646
            253-293             AC162789.4         152089-152129
            294-378             AC162789.4         155356-155440
            379-490             AC162789.4         156602-156713
            491-806             AC162789.4         158038-158353
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 72.8 cM"
     gene            1..806
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /note="mitochondrial pyruvate carrier 2"
                     /db_xref="GeneID:70456"
                     /db_xref="MGI:MGI:1917706"
     exon            1..252
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /inference="alignment:Splign:2.1.0"
     CDS             144..527
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /note="brain protein 44"
                     /codon_start=1
                     /product="mitochondrial pyruvate carrier 2"
                     /protein_id="NP_081706.1"
                     /db_xref="CCDS:CCDS15442.1"
                     /db_xref="GeneID:70456"
                     /db_xref="MGI:MGI:1917706"
                     /translation="MAAAGARGLRATYHRLMDKVELLLPKKLRPLYNHPAGPRTVFFW
                     APIMKWGLVCAGLADMARPAEKLSTAQSTVLMATGFIWSRYSLVIIPKNWSLFAVNFF
                     VGSAGASQLFRIWRYNQELKSKGIQ"
     exon            253..293
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /inference="alignment:Splign:2.1.0"
     exon            294..378
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /inference="alignment:Splign:2.1.0"
     exon            379..490
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /inference="alignment:Splign:2.1.0"
     exon            491..806
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /inference="alignment:Splign:2.1.0"
     regulatory      686..691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /note="hexamer: AATAAA"
     polyA_site      708
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /note="major polyA site"
     regulatory      782..787
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
                     /note="hexamer: AATAAA"
     polyA_site      804
                     /gene="Mpc2"
                     /gene_synonym="0610006C01Rik; 2010002I07Rik;
                     2610205H19Rik; Brp44; ESTM43"
ORIGIN      
        1 agagccgggg aagggcgccc tagggcagag gcggacgccc cagcgctgca gagagaaagt
       61 gacgagccgc ccttcgttgt cagccaggaa cgcggacaca gcggcgctcc caccctgctg
      121 ctgtcgatcc ctaggccgcc gcgatggcag ctgccggcgc ccgaggcctg cgggccacct
      181 accaccgact catggataaa gtggagttgt tgctgccaaa gaaattgagg ccgctttaca
      241 accacccggc aggccccaga acagtttttt tctgggctcc aataatgaaa tggggattgg
      301 tgtgtgctgg actagcggac atggccagac ctgcagagaa actcagcaca gctcagtcca
      361 ctgtgttgat ggctacaggg tttatttggt caagatattc acttgtaatt ataccaaaaa
      421 attggagttt gttcgctgtt aatttctttg tggggtcagc aggagcctct cagctgtttc
      481 ggatttggag atataaccaa gaactcaaat ctaaagggat ccagtaagag agttcctgat
      541 cacctgaaca ttctagatgt ggacagaaac attggaacct agtgtatttg cttaccgata
      601 aggtgatgct aaattctgat aacattcgaa aagaaggaaa gcaaaatcta actgtactta
      661 aaagctagca cttgacttca caaaaaataa agcaaacttt tagtaccagt ctgtctttac
      721 atttgcctca aggggacttg cttgtggatg gcaccaacct tggtttctac catttgtcca
      781 taataaaccg tacttggtca tgcata
//
