LOCUS       NM_001410429            1219 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus meiosis-specific nuclear structural protein 1 (Mns1),
            transcript variant 2, mRNA.
ACCESSION   NM_001410429 XM_006510824
VERSION     NM_001410429.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Lu,H., Twan,W.K., Ikawa,Y., Khare,V., Mukherjee,I., Schou,K.B.,
            Chua,K.X., Aqasha,A., Chakrabarti,S., Hamada,H. and Roy,S.
  TITLE     Localisation and function of key axonemal microtubule inner
            proteins and dynein docking complex members reveal extensive
            diversity among vertebrate motile cilia
  JOURNAL   Development 151 (14) (2024)
   PUBMED   39007638
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Tai,L., Yin,G., Huang,X., Sun,F. and Zhu,Y.
  TITLE     In-cell structural insight into the stability of sperm microtubule
            doublet
  JOURNAL   Cell Discov 9 (1), 116 (2023)
   PUBMED   37989994
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1219)
  AUTHORS   Chen,Z., Shiozaki,M., Haas,K.M., Skinner,W.M., Zhao,S., Guo,C.,
            Polacco,B.J., Yu,Z., Krogan,N.J., Lishko,P.V., Kaake,R.M.,
            Vale,R.D. and Agard,D.A.
  TITLE     De novo protein identification in mammalian sperm using in situ
            cryoelectron tomography and AlphaFold2 docking
  JOURNAL   Cell 186 (23), 5041-5053 (2023)
   PUBMED   37865089
REFERENCE   4  (bases 1 to 1219)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   5  (bases 1 to 1219)
  AUTHORS   Cao,H., Xu,H., Zhou,Y., Xu,W., Lu,Q., Jiang,L., Rong,Y., Zhang,Q.
            and Yu,C.
  TITLE     BBOF1 is required for sperm motility and male fertility by
            stabilizing the flagellar axoneme in mice
  JOURNAL   Cell Mol Life Sci 80 (6), 152 (2023)
   PUBMED   37198331
  REMARK    GeneRIF: BBOF1 is required for sperm motility and male fertility by
            stabilizing the flagellar axoneme in mice.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1219)
  AUTHORS   Boschen,K.E., Gong,H., Murdaugh,L.B. and Parnell,S.E.
  TITLE     Knockdown of Mns1 Increases Susceptibility to Craniofacial Defects
            Following Gastrulation-Stage Alcohol Exposure in Mice
  JOURNAL   Alcohol Clin Exp Res 42 (11), 2136-2143 (2018)
   PUBMED   30129265
  REMARK    GeneRIF: Prenatal alcohol exposure Mns1(-/-) fetuses with severe
            eye defects also presented with craniofacial dysmorphologies
            characteristic of fetal alcohol syndrome and midline tissue loss in
            the brain, palate, and nasal septum
REFERENCE   7  (bases 1 to 1219)
  AUTHORS   Vadnais,M.L., Lin,A.M. and Gerton,G.L.
  TITLE     Mitochondrial fusion protein MFN2 interacts with the
            mitostatin-related protein MNS1 required for mouse sperm flagellar
            structure and function
  JOURNAL   Cilia 3, 5 (2014)
   PUBMED   24876927
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1219)
  AUTHORS   Zhou,J., Yang,F., Leu,N.A. and Wang,P.J.
  TITLE     MNS1 is essential for spermiogenesis and motile ciliary functions
            in mice
  JOURNAL   PLoS Genet 8 (3), e1002516 (2012)
   PUBMED   22396656
  REMARK    GeneRIF: These results demonstrate that MNS1 is essential for
            spermiogenesis, the assembly of sperm flagella, and motile ciliary
            functions.
            GeneRIF: MNS1 is an integral component of sperm flagella. Mice
            lacking MNS1 exhibit male sterility as evidenced by abnormal
            assembly of flagella. MNS1-deficient mice also display defects in
            left-right asymmetry patterning of internal organs and
            hydrocephalus.
REFERENCE   9  (bases 1 to 1219)
  AUTHORS   Schwab,K., Patterson,L.T., Aronow,B.J., Luckas,R., Liang,H.C. and
            Potter,S.S.
  TITLE     A catalogue of gene expression in the developing kidney
  JOURNAL   Kidney Int 64 (5), 1588-1604 (2003)
   PUBMED   14531791
REFERENCE   10 (bases 1 to 1219)
  AUTHORS   Furukawa,K., Inagaki,H., Naruge,T., Tabata,S., Tomida,T.,
            Yamaguchi,A., Yoshikuni,M., Nagahama,Y. and Hotta,Y.
  TITLE     cDNA cloning and functional characterization of a meiosis-specific
            protein (MNS1) with apparent nuclear association
  JOURNAL   Chromosome Res 2 (2), 99-113 (1994)
   PUBMED   8032679
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CT954326.5 and AC111135.10.
            
            On Aug 8, 2022 this sequence version replaced XM_006510824.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR13861890.12252.1,
                                           SRR5189685.45409.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-118               CT954326.5         136596-136713
            119-340             CT954326.5         137274-137495
            341-468             AC111135.10        110748-110875
            469-571             AC111135.10        112112-112214
            572-829             AC111135.10        118486-118743
            830-955             AC111135.10        122683-122808
            956-1219            AC111135.10        124218-124481
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 40.08 cM"
     gene            1..1219
                     /gene="Mns1"
                     /note="meiosis-specific nuclear structural protein 1"
                     /db_xref="GeneID:17427"
                     /db_xref="MGI:MGI:107933"
     exon            1..118
                     /gene="Mns1"
                     /inference="alignment:Splign:2.1.0"
     CDS             116..1036
                     /gene="Mns1"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="meiosis-specific nuclear structural protein 1
                     isoform 2"
                     /protein_id="NP_001397358.1"
                     /db_xref="GeneID:17427"
                     /db_xref="MGI:MGI:107933"
                     /translation="MATKKRALSFSEKHQKLVDEKFRKSLNIQVMNKLERQAKNQVVQ
                     NENDEKVERQRFLRVLQNEQFELDMEEAIQKAEANKMLRDRQLEQEERLANELARLKH
                     ESLKDKKMRQQVRENSIELRELEQKLKAAYMNKERAAQIVEKDAMKYEQMEEAELRLR
                     RQREMKQDFEDQMALKELILQAAKEEEETFKKAMLAKFAEDDRIELMNAQKQRMKQLE
                     HKRAVEKLIEERRSQFLADKQRELEELQLQQRRQGCINEIIEEERLRLLKEHAAKLLG
                     YLPKGVFKREDDVDMLGEEFRKAYQKRDGV"
     exon            119..340
                     /gene="Mns1"
                     /inference="alignment:Splign:2.1.0"
     exon            341..468
                     /gene="Mns1"
                     /inference="alignment:Splign:2.1.0"
     exon            469..571
                     /gene="Mns1"
                     /inference="alignment:Splign:2.1.0"
     exon            572..829
                     /gene="Mns1"
                     /inference="alignment:Splign:2.1.0"
     exon            830..955
                     /gene="Mns1"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1219
                     /gene="Mns1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1198..1203
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mns1"
                     /note="hexamer: ATTAAA"
     polyA_site      1219
                     /gene="Mns1"
                     /note="major polyA site"
ORIGIN      
        1 aaacttaacc cagcgtgcac aggtatccgc tgccgtggct gctgctccgg aagacgcgtt
       61 tgcaactgag gcagagaccc atcctgccgg aggaggaggt ttaaagccaa ccgagatggc
      121 gaccaaaaag agagctttga gctttagtga aaagcatcag aagttagtag atgaaaaatt
      181 ccgcaaatca ttgaacatac aagtcatgaa taagttagag aggcaagcca agaaccaagt
      241 ggtgcaaaat gaaaacgacg agaaggtgga acgccagagg ttcctcagag tgctgcagaa
      301 tgagcagttt gagctcgaca tggaagaagc catccaaaaa gcagaagcaa acaagatgct
      361 gagggatcga cagcttgagc aggaagagag acttgcaaac gaactggcca gactgaagca
      421 cgagagtctg aaggataaga agatgaggca gcaagtgaga gagaacagca ttgagctcag
      481 agagttggag cagaaactga aagccgccta catgaacaaa gagagagcag cccagatcgt
      541 ggagaaggac gccatgaagt atgagcagat ggaagaagca gagctgagac tgaggaggca
      601 gagggagatg aagcaagact ttgaagacca aatggcactg aaggaactca tactgcaggc
      661 tgcaaaagag gaggaagaga cctttaagaa agccatgctg gccaagtttg ccgaagatga
      721 ccgcattgag ctcatgaatg cccagaaaca gcgaatgaag cagctggagc acaaacgcgc
      781 cgtggagaaa ctcattgaag aacgccggag ccagttcctc gcagacaaac aacgtgagct
      841 ggaggaattg caactacagc aaaggcggca gggctgcatc aatgagatta ttgaagaaga
      901 gaggctgaga ctcctcaaag aacatgctgc aaagttacta ggctatctcc ccaaaggagt
      961 gtttaaaaga gaagacgatg ttgacatgct tggtgaagag ttcaggaagg cctatcagaa
     1021 aagggatgga gtctgaagcc gagtcttcat agcaaggccg ggcgagacct ggagtgctca
     1081 gatgctgcca ctagatgtca gcgtaactgc tgttttacag ttggtgacag taggccctgg
     1141 tctatctgca tgttctaaaa catcctccta tttcatcatg tatacagttt gacaaaaatt
     1201 aaacacatta gtactttaa
//
