LOCUS       NM_013599               3152 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus matrix metallopeptidase 9 (Mmp9), mRNA.
ACCESSION   NM_013599
VERSION     NM_013599.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Feng,W., Hong,N., Wu,Y., Huang,J., Zhang,Q., Liu,G., Qian,Z.,
            Chen,Y., Jin,L., Ding,X., Zhao,P., Chen,A.F. and Yu,Y.
  TITLE     Deficiency of Sox7 leads to congenital aortic stenosis via abnormal
            valve remodeling
  JOURNAL   J Mol Cell Cardiol 199, 81-94 (2025)
   PUBMED   39746830
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Janeckova,E., Juarez-Balarezo,J., Tucker,A.S., Matalova,E.,
            Holomkova,K. and Gaete,M.
  TITLE     Metalloproteinases are involved in the regulation of prenatal tooth
            morphogenesis
  JOURNAL   Am J Physiol Cell Physiol 328 (1), C323-C333 (2025)
   PUBMED   39510136
REFERENCE   3  (bases 1 to 3152)
  AUTHORS   Houchen,C.J., Ghanem,S., Kaartinen,V. and Bumann,E.E.
  TITLE     TGF-beta signaling in the cranial neural crest affects late-stage
            mandibular bone resorption and length
  JOURNAL   Front Physiol 15, 1435594 (2024)
   PUBMED   39473613
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3152)
  AUTHORS   Ishibashi,R., Itani,M., Kawashima,A., Arakawa,Y. and Aoki,T.
  TITLE     JNK2-MMP-9 axis facilitates the progression of intracranial
            aneurysms
  JOURNAL   Sci Rep 14 (1), 19458 (2024)
   PUBMED   39169203
  REMARK    GeneRIF: JNK2-MMP-9 axis facilitates the progression of
            intracranial aneurysms.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3152)
  AUTHORS   Zhu,Z., Zou,Q., Wang,C., Li,D., Yang,Y., Xiao,Y., Jin,Y., Yan,J.,
            Luo,L., Sun,Y. and Liang,X.
  TITLE     Isl Identifies the Extraembryonic Mesodermal/Allantois Progenitors
            and is Required for Placenta Morphogenesis and Vasculature
            Formation
  JOURNAL   Adv Sci (Weinh) 11 (32), e2400238 (2024)
   PUBMED   38923264
REFERENCE   6  (bases 1 to 3152)
  AUTHORS   Canete Soler,R., Gui,Y.H., Linask,K.K. and Muschel,R.J.
  TITLE     MMP-9 (gelatinase B) mRNA is expressed during mouse neurogenesis
            and may be associated with vascularization
  JOURNAL   Brain Res Dev Brain Res 88 (1), 37-52 (1995)
   PUBMED   7493406
REFERENCE   7  (bases 1 to 3152)
  AUTHORS   Harvey,M.B., Leco,K.J., Arcellana-Panlilio,M.Y., Zhang,X.,
            Edwards,D.R. and Schultz,G.A.
  TITLE     Proteinase expression in early mouse embryos is regulated by
            leukaemia inhibitory factor and epidermal growth factor
  JOURNAL   Development 121 (4), 1005-1014 (1995)
   PUBMED   7743917
REFERENCE   8  (bases 1 to 3152)
  AUTHORS   Reponen,P., Leivo,I., Sahlberg,C., Apte,S.S., Olsen,B.R.,
            Thesleff,I. and Tryggvason,K.
  TITLE     92-kDa type IV collagenase and TIMP-3, but not 72-kDa type IV
            collagenase or TIMP-1 or TIMP-2, are highly expressed during mouse
            embryo implantation
  JOURNAL   Dev Dyn 202 (4), 388-396 (1995)
   PUBMED   7626795
REFERENCE   9  (bases 1 to 3152)
  AUTHORS   Reponen,P., Sahlberg,C., Munaut,C., Thesleff,I. and Tryggvason,K.
  TITLE     High expression of 92-kD type IV collagenase (gelatinase B) in the
            osteoclast lineage during mouse development
  JOURNAL   J Cell Biol 124 (6), 1091-1102 (1994)
   PUBMED   8132709
REFERENCE   10 (bases 1 to 3152)
  AUTHORS   Graubert,T., Johnston,J. and Berliner,N.
  TITLE     Cloning and expression of the cDNA encoding mouse neutrophil
            gelatinase: demonstration of coordinate secondary granule protein
            gene expression during terminal neutrophil maturation
  JOURNAL   Blood 82 (10), 3192-3197 (1993)
   PUBMED   8219207
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL591495.12.
            
            On Apr 1, 2021 this sequence version replaced NM_013599.4.
            
            Summary: This gene encodes a member of the matrix metalloproteinase
            family of extracellular matrix-degrading enzymes that are involved
            in tissue remodeling, wound repair, progression of atherosclerosis
            and tumor invasion. The encoded preproprotein undergoes proteolytic
            processing to generate a mature, zinc-dependent endopeptidase
            enzyme that degrades collagens of type IV, V and XI, and elastin.
            Mice lacking the encoded protein exhibit an abnormal pattern of
            skeletal growth plate vascularization and ossification, reduced
            keratinocyte hyperproliferation at all neoplastic stages, a
            decreased incidence of invasive tumors, and resistance to
            experimental autoimmune encephalomyelitis. [provided by RefSeq, Feb
            2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: Z27231.1, BC046991.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-160               AL591495.12        130857-131016
            161-390             AL591495.12        131454-131683
            391-539             AL591495.12        131902-132050
            540-668             AL591495.12        132328-132456
            669-842             AL591495.12        132566-132739
            843-1016            AL591495.12        132920-133093
            1017-1193           AL591495.12        133444-133620
            1194-1349           AL591495.12        133726-133881
            1350-1683           AL591495.12        135039-135372
            1684-1823           AL591495.12        135474-135613
            1824-1974           AL591495.12        135883-136033
            1975-2078           AL591495.12        136138-136241
            2079-3152           AL591495.12        137392-138465
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 85.27 cM"
     gene            1..3152
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /note="matrix metallopeptidase 9"
                     /db_xref="GeneID:17395"
                     /db_xref="MGI:MGI:97011"
     exon            1..160
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     CDS             20..2212
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /EC_number="3.4.24.35"
                     /note="collagenase type IVB; 92 kDa gelatinase; 92 kDa
                     type IV collagenase; matrix metalloproteinase-9; 92kD
                     gelatinase; 92kD type IV collagenase; gelatinase B"
                     /codon_start=1
                     /product="matrix metalloproteinase-9 preproprotein"
                     /protein_id="NP_038627.1"
                     /db_xref="CCDS:CCDS17066.1"
                     /db_xref="GeneID:17395"
                     /db_xref="MGI:MGI:97011"
                     /translation="MSPWQPLLLALLAFGCSSAAPYQRQPTFVVFPKDLKTSNLTDTQ
                     LAEAYLYRYGYTRAAQMMGEKQSLRPALLMLQKQLSLPQTGELDSQTLKAIRTPRCGV
                     PDVGRFQTFKGLKWDHHNITYWIQNYSEDLPRDMIDDAFARAFAVWGEVAPLTFTRVY
                     GPEADIVIQFGVAEHGDGYPFDGKDGLLAHAFPPGAGVQGDAHFDDDELWSLGKGVVI
                     PTYYGNSNGAPCHFPFTFEGRSYSACTTDGRNDGTPWCSTTADYDKDGKFGFCPSERL
                     YTEHGNGEGKPCVFPFIFEGRSYSACTTKGRSDGYRWCATTANYDQDKLYGFCPTRVD
                     ATVVGGNSAGELCVFPFVFLGKQYSSCTSDGRRDGRLWCATTSNFDTDKKWGFCPDQG
                     YSLFLVAAHEFGHALGLDHSSVPEALMYPLYSYLEGFPLNKDDIDGIQYLYGRGSKPD
                     PRPPATTTTEPQPTAPPTMCPTIPPTAYPTVGPTVGPTGAPSPGPTSSPSPGPTGAPS
                     PGPTAPPTAGSSEASTESLSPADNPCNVDVFDAIAEIQGALHFFKDGWYWKFLNHRGS
                     PLQGPFLTARTWPALPATLDSAFEDPQTKRVFFFSGRQMWVYTGKTVLGPRSLDKLGL
                     GPEVTHVSGLLPRRLGKALLFSKGRVWRFDLKSQKVDPQSVIRVDKEFSGVPWNSHDI
                     FQYQDKAYFCHGKFFWRVSFQNEVNKVDHEVNQVDDVGYVTYDLLQCP"
     exon            161..390
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            391..539
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            540..668
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            669..842
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            843..1016
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1017..1193
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1349
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1350..1683
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1684..1823
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1974
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            1975..2078
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     exon            2079..3152
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2411..2416
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /note="hexamer: AATAAA"
     polyA_site      2442
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /note="major polyA site"
     regulatory      3125..3130
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /note="hexamer: AATAAA"
     polyA_site      3152
                     /gene="Mmp9"
                     /gene_synonym="B/MMP9; Clg4b; Gel B; MMP-9; pro-MMP-9"
                     /note="major polyA site"
ORIGIN      
        1 agaagctgcg gtcctcacca tgagtccctg gcagcccctg ctcctggctc tcctggcttt
       61 cggctgcagc tctgctgccc cttaccagcg ccagccgact tttgtggtct tccccaaaga
      121 cctgaaaacc tccaacctca cggacaccca gctggcagag gcatacttgt accgctatgg
      181 ttacacccgg gccgcccaga tgatgggaga gaagcagtct ctacggccgg ctttgctgat
      241 gcttcagaag cagctctccc tgccccagac tggtgagctg gacagccaga cactaaaggc
      301 cattcgaaca ccacgctgtg gtgtcccaga cgtgggtcga ttccaaacct tcaaaggcct
      361 caagtgggac catcataaca tcacatactg gatccaaaac tactctgaag acttgccgcg
      421 agacatgatc gatgacgcct tcgcgcgcgc cttcgcggtg tggggcgagg tggcacccct
      481 caccttcacc cgcgtgtacg gacccgaagc ggacattgtc atccagtttg gtgtcgcgga
      541 gcacggagac gggtatccct tcgacggcaa ggacggcctt ctggcacacg cctttccccc
      601 tggcgccggc gttcagggag atgcccattt cgacgacgac gagttgtggt cgctgggcaa
      661 aggcgtcgtg atccccactt actatggaaa ctcaaatggt gccccatgtc actttccctt
      721 caccttcgag ggacgctcct attcggcctg caccacagac ggccgcaacg acggcacgcc
      781 ttggtgtagc acaacagctg actacgataa ggacggcaaa tttggtttct gccctagtga
      841 gagactctac acggagcacg gcaacggaga aggcaaaccc tgtgtgttcc cgttcatctt
      901 tgagggccgc tcctactctg cctgcaccac taaaggccgc tcggatggtt accgctggtg
      961 cgccaccaca gccaactatg accaggataa actgtatggc ttctgcccta cccgagtgga
     1021 cgcgaccgta gttgggggca actcggcagg agagctgtgc gtcttcccct tcgtcttcct
     1081 gggcaagcag tactcttcct gtaccagcga cggccgcagg gatgggcgcc tctggtgtgc
     1141 gaccacatcg aacttcgaca ctgacaagaa gtggggtttc tgtccagacc aagggtacag
     1201 cctgttcctg gtggcagcgc acgagttcgg ccatgcactg ggcttagatc attccagcgt
     1261 gccggaagcg ctcatgtacc cgctgtatag ctacctcgag ggcttccctc tgaataaaga
     1321 cgacatagac ggcatccagt atctgtatgg tcgtggctct aagcctgacc caaggcctcc
     1381 agccaccacc acaactgaac cacagccgac agcacctccc actatgtgtc ccactatacc
     1441 tcccacggcc tatcccacag tgggccccac ggttggccct acaggcgccc cctcacctgg
     1501 ccccacaagc agcccgtcac ctggccctac aggcgccccc tcacctggcc ctacagcgcc
     1561 ccctactgcg ggctcttctg aggcctctac agagtctttg agtccggcag acaatccttg
     1621 caatgtggat gtttttgatg ctattgctga gatccagggc gctctgcatt tcttcaagga
     1681 cggttggtac tggaagttcc tgaatcatag aggaagccca ttacagggcc ccttccttac
     1741 tgcccgcacg tggccagccc tgcctgcaac gctggactcc gcctttgagg atccgcagac
     1801 caagagggtt ttcttcttct ctggacgtca aatgtgggtg tacacaggca agaccgtgct
     1861 gggccccagg agtctggata agttgggtct aggcccagag gtaacccacg tcagcgggct
     1921 tctcccgcgt cgtctcggga aggctctgct gttcagcaag gggcgtgtct ggagattcga
     1981 cttgaagtct cagaaggtgg atccccagag cgtcattcgc gtggataagg agttctctgg
     2041 tgtgccctgg aactcacacg acatcttcca gtaccaagac aaagcctatt tctgccatgg
     2101 caaattcttc tggcgtgtga gtttccaaaa tgaggtgaac aaggtggacc atgaggtgaa
     2161 ccaggtggac gacgtgggct acgtgaccta cgacctcctg cagtgccctt gaactagggc
     2221 tccttctttg cttcaaccgt gcagtgcaag tctctagaga ccaccaccac caccaccaca
     2281 cacaaacccc atccgaggga aaggtgctag ctggccaggt acagactggt gatctcttct
     2341 agagactggg aaggagtgga ggcaggcagg gctctctctg cccaccgtcc tttcttgttg
     2401 gactgtttct aataaacacg gatccccaac cttttccagc tactttagtc aatcagctta
     2461 tctgtagttg cagatgcatc cgagcaagaa gacaactttg tagggtggat tctgaccttt
     2521 tatttttgtg tggcgtctga gaattgaatc agctggcttt tgtgacaggc acttcaccgg
     2581 ctaaaccacc tctcccgact ccagcccttt tatttattat gtatgaggtt atgttcacat
     2641 gcatgtattt aacccacaga atgcttactg tgtgtcgggc gcggctccaa ccgctgcata
     2701 aatattaagg tattcagttg cccctactgg aaggtattat gtaactattt ctctcttaca
     2761 ttggagaaca ccaccgagct atccactcat caaacattta ttgagagcat ccctagggag
     2821 ccaggctctc tactgggcgt tagggacaga aatgttggtt cttccttcaa ggattgctca
     2881 gagattctcc gtgtcctgta aatctgctga aaccagaccc cagactcctc tctctcccga
     2941 gagtccaact cactcactgt ggttgctggc agctgcagca tgcgtataca gcatgtgtgc
     3001 tagagaggta gagggggtct gtgcgttatg gttcaggtca gactgtgtcc tccaggtgag
     3061 atgacccctc agctggaact gatccaggaa ggataaccaa gtgtcttcct ggcagtcttt
     3121 tttaaataaa tgaataaatg aatatttact ta
//
