LOCUS       NM_013903               3276 bp    mRNA    linear   ROD 30-JUL-2024
DEFINITION  Mus musculus matrix metallopeptidase 20 (enamelysin) (Mmp20), mRNA.
ACCESSION   NM_013903
VERSION     NM_013903.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Shin,M., Matsushima,A., Nagao,J.I., Tanaka,Y., Harada,H., Okabe,K.
            and Bartlett,J.D.
  TITLE     Mobility gene expression differences among wild-type, Mmp20 null
            and Mmp20 over-expresser mice plus visualization of 3D mouse
            ameloblast directional movement
  JOURNAL   Sci Rep 13 (1), 18829 (2023)
   PUBMED   37914726
  REMARK    GeneRIF: Mobility gene expression differences among wild-type,
            Mmp20 null and Mmp20 over-expresser mice plus visualization of 3D
            mouse ameloblast directional movement.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3276)
  AUTHORS   Fan,L., Ou,Y.J., Zhu,Y.X., Liang,Y.D., Zhou,Y. and Wang,Y.N.
  TITLE     Lif Deficiency Leads to Iron Transportation Dysfunction in
            Ameloblasts
  JOURNAL   J Dent Res 101 (1), 63-72 (2022)
   PUBMED   34034544
REFERENCE   4  (bases 1 to 3276)
  AUTHORS   Bartlett,J.D., Smith,C.E., Hu,Y., Ikeda,A., Strauss,M., Liang,T.,
            Hsu,Y.H., Trout,A.H., McComb,D.W., Freeman,R.C., Simmer,J.P. and
            Hu,J.C.
  TITLE     MMP20-generated amelogenin cleavage products prevent formation of
            fan-shaped enamel malformations
  JOURNAL   Sci Rep 11 (1), 10570 (2021)
   PUBMED   34012043
  REMARK    GeneRIF: MMP20-generated amelogenin cleavage products prevent
            formation of fan-shaped enamel malformations.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3276)
  AUTHORS   Kim,E.J., Lee,J.E., Yoon,S., Lee,D.J., Mai,H.N., Ida-Yonemochi,H.,
            Choi,J. and Jung,H.S.
  TITLE     Hypoxia-Responsive Oxygen Nanobubbles for Tissues-Targeted Delivery
            in Developing Tooth Germs
  JOURNAL   Front Cell Dev Biol 9, 626224 (2021)
   PUBMED   33659251
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3276)
  AUTHORS   Hu,J.C., Sun,X., Zhang,C., Liu,S., Bartlett,J.D. and Simmer,J.P.
  TITLE     Enamelysin and kallikrein-4 mRNA expression in developing mouse
            molars
  JOURNAL   Eur J Oral Sci 110 (4), 307-315 (2002)
   PUBMED   12206593
  REMARK    GeneRIF: expression of MMP-20 correlates with the stage-associated
            changes in the digestion of enamel proteins
REFERENCE   7  (bases 1 to 3276)
  AUTHORS   Caterina,J., Shi,J., Sun,X., Qian,Q., Yamada,S., Liu,Y.,
            Krakora,S., Bartlett,J.D., Yamada,Y., Engler,J.A.,
            Birkedal-Hansen,H. and Simmer,J.P.
  TITLE     Cloning, characterization, and expression analysis of mouse
            enamelysin
  JOURNAL   J Dent Res 79 (9), 1697-1703 (2000)
   PUBMED   11023266
REFERENCE   8  (bases 1 to 3276)
  AUTHORS   Hrabe de Angelis,M.H., Flaswinkel,H., Fuchs,H., Rathkolb,B.,
            Soewarto,D., Marschall,S., Heffner,S., Pargent,W., Wuensch,K.,
            Jung,M., Reis,A., Richter,T., Alessandrini,F., Jakob,T., Fuchs,E.,
            Kolb,H., Kremmer,E., Schaeble,K., Rollinski,B., Roscher,A.,
            Peters,C., Meitinger,T., Strom,T., Steckler,T., Holsboer,F.,
            Klopstock,T., Gekeler,F., Schindewolf,C., Jung,T., Avraham,K.,
            Behrendt,H., Ring,J., Zimmer,A., Schughart,K., Pfeffer,K., Wolf,E.
            and Balling,R.
  TITLE     Genome-wide, large-scale production of mutant mice by ENU
            mutagenesis
  JOURNAL   Nat Genet 25 (4), 444-447 (2000)
   PUBMED   10932192
REFERENCE   9  (bases 1 to 3276)
  AUTHORS   Johansson,N., Ahonen,M. and Kahari,V.M.
  TITLE     Matrix metalloproteinases in tumor invasion
  JOURNAL   Cell Mol Life Sci 57 (1), 5-15 (2000)
   PUBMED   10949577
  REMARK    Review article
REFERENCE   10 (bases 1 to 3276)
  AUTHORS   Caterina,J., Shi,J., Krakora,S., Bartlett,J.D., Engler,J.A.,
            Kozak,C.A. and Birkedal-Hansen,H.
  TITLE     Isolation, characterization, and chromosomal location of the mouse
            enamelysin gene
  JOURNAL   Genomics 62 (2), 308-311 (1999)
   PUBMED   10610728
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL603630.11.
            
            On Nov 17, 2006 this sequence version replaced NM_013903.1.
            
            Summary: This gene encodes a member of the matrix metalloproteinase
            family of zinc-dependent endopeptidases capable of degrading
            extracellular matrix proteins. This gene is expressed specifically
            in the ameloblasts and odontoblasts, and the encoded protein is an
            inactive zymogen that requires proteolytic removal of a N-terminal
            propeptide to become enzymatically active. Mice lacking the encoded
            protein display an amelogenesis imperfecta phenotype. [provided by
            RefSeq, Oct 2015].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF155933.1, BC152336.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-145               AL603630.11        73313-73457
            146-393             AL603630.11        80225-80472
            394-542             AL603630.11        84286-84434
            543-668             AL603630.11        87769-87894
            669-830             AL603630.11        89041-89202
            831-972             AL603630.11        90341-90482
            973-1109            AL603630.11        99113-99249
            1110-1266           AL603630.11        100062-100218
            1267-1370           AL603630.11        116425-116528
            1371-3276           AL603630.11        118145-120050
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 2.46 cM"
     gene            1..3276
                     /gene="Mmp20"
                     /note="matrix metallopeptidase 20 (enamelysin)"
                     /db_xref="GeneID:30800"
                     /db_xref="MGI:MGI:1353466"
     exon            1..145
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
     CDS             23..1471
                     /gene="Mmp20"
                     /note="matrix metalloproteinase-20; enamel
                     metalloproteinase; matrix metalloproteinase 20
                     (enamelysin)"
                     /codon_start=1
                     /product="matrix metalloproteinase-20 preproprotein"
                     /protein_id="NP_038931.1"
                     /db_xref="CCDS:CCDS22809.1"
                     /db_xref="GeneID:30800"
                     /db_xref="MGI:MGI:1353466"
                     /translation="MKVLPASGLAVLVTALKFATADPNLLAATPRTFRSNYHLAQAYL
                     DKYYTKKGGPQAGEMVARESNPMIRRIKELQIFFGLKVTGKLDQNTMNVIKKPRCGVP
                     DVANYRLFPGEPKWKKNILTYRISKYTPSMSPTEVDKAIQMALHAWSTAVPLNFVRIN
                     SGEADIMISFETGDHGDSYPFDGPRGTLAHAFAPGEGLGGDTHFDNAEKWTMGTNGFN
                     LFTVAAHEFGHALGLGHSTDPSALMYPTYKYQNPYRFHLPKDDVKGIQALYGPRKIFP
                     GKPTMPHIPPHKPSIPDLCDSSSSFDAVTMLGKELLFFKDRIFWRRQVHLPTGIRPST
                     ITSSFPQLMSNVDAAYEVAERGIAFFFKGPHYWVTRGFHMQGPPRTIYDFGFPRHVQR
                     IDAAVYLKEPQKTLFFVGEEYYSYDERKKKMEKDYPKNTEEEFSGVSGHIDAAVELNG
                     YIYFFSGRKTFKYDTEKEDVVSVVKSSSWIGC"
     exon            146..393
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
     exon            394..542
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
     exon            543..668
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
     exon            669..830
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
     exon            831..972
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
     exon            973..1109
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1266
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1370
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..3276
                     /gene="Mmp20"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 caatcatcaa gcaagagagg agatgaaggt gctacctgcc tctggccttg ctgtccttgt
       61 cacagcttta aagtttgcca ctgcagaccc caacctactg gcagccaccc ctaggacctt
      121 caggagcaac taccaccttg cacaggccta tcttgacaag tattatacaa agaaaggagg
      181 cccccaggct ggcgagatgg tggcaagaga aagcaacccc atgatcagga ggattaagga
      241 gctacaaata ttctttggcc tcaaagtcac tgggaagttg gaccagaata caatgaatgt
      301 gatcaagaag cctcgctgtg gagttcctga tgtggctaac taccgcctct tcccaggtga
      361 acccaaatgg aaaaaaaata ttttgacata cagaatctct aagtacacac cctccatgag
      421 tcccactgag gtggacaagg caatacagat ggccctgcat gcgtggagta cagctgtccc
      481 tctgaacttc gttagaataa actctgggga agcagatatc atgatatctt ttgaaactgg
      541 agatcatggg gattcctacc catttgatgg gcctcgaggg actctggccc atgcatttgc
      601 tcctggagaa ggcctgggag gagacacaca tttcgacaat gctgagaagt ggactatggg
      661 aacgaatggg ttcaatttat tcaccgttgc tgctcatgaa tttggccatg ctctgggcct
      721 gggccattcc acagatccat cagcactgat gtacccaact tataagtacc agaaccccta
      781 tagatttcac ctccccaagg atgatgtgaa agggatccaa gcattatacg gaccccggaa
      841 aatattccca ggaaagccca ccatgcccca tatcccacct cacaagccat ctatccccga
      901 cctctgtgac tccagctcgt cctttgatgc agtgacaatg ttgggaaagg agcttctgtt
      961 cttcaaggat aggattttct ggagaaggca ggtgcacttg ccaacaggga ttcggcccag
     1021 caccatcacc agctcctttc cccagctaat gtccaatgtg gacgcggctt acgaagtggc
     1081 tgaacgaggc attgctttct tcttcaaagg tcctcactac tgggtcactc gaggattcca
     1141 tatgcaaggt cctccacgga caatttatga cttcgggttc ccaaggcatg tgcaacgaat
     1201 agatgctgct gtctacctca aggagcctca gaagaccctt ttctttgtgg gagaggaata
     1261 ctacagctat gatgaaagaa aaaagaaaat ggaaaaagac tacccaaaaa acactgaaga
     1321 agagttttca ggagtgagtg gccatataga tgctgctgtg gaactgaatg gctacattta
     1381 cttcttttca ggacggaaaa cattcaagta tgatacagag aaggaagacg tggttagtgt
     1441 ggtaaaatcc agttcctgga ttggctgctg agtcgtcagg tctctccgca gggacgaaga
     1501 gagctgtgag caactgatga ctggacatta cgcaccaagt cgtgtgtcag agacactcct
     1561 ctccaacctt cagtcgtgag ggtgattgaa ggttccttga aaataataat tctattgttt
     1621 ttcctaatat tcattcacaa ttctagtcta aatgagaaac cacctttatt caaaatcgca
     1681 taatataccc tttagttaaa tattcacttt tctcttacct actgtagtaa agcaaattga
     1741 tcactttggt ttctgtttgt agaaaaagga ctatgtgcac tcatgtgtgt gcgcatacac
     1801 acacgtgctt atgaacacat acacacatac aaatatttca ttaagcaagg tcattaccat
     1861 acaaattaaa attactcttt gaaaaataat ggccatcatt tactatgatg gacataaatg
     1921 tacttatttg tgctttgaag gtatcacaaa cgaaatgtac caccaggtgt gttcttggct
     1981 ctagctgtcc tctttgtctc ctactggcct ctgaagaggg ttggtagaca aacattcaaa
     2041 ttaatcttca tgctcacaac agccagacac tctacaacag ggagaacttt tctaacaaaa
     2101 cccaataaaa aatgttgggt tagaggagtt cctcatttct cttttctgtt aatatttact
     2161 agctcttatc atgctttatt tggcgacaaa ttctgtgtac ttgtagaaaa tgtaaatgat
     2221 agtatcgaat gtacacagat ccaggaaaaa taacctagtc tgatctattt cctacttcct
     2281 ggtagcacct gcagtggaga tctttgatcc acctcagttt cttctaaata gttttctact
     2341 taatgaagtc tattttttgt tgtttttgtt tgtttgtttg ttttaattca aactaaacct
     2401 tttcttccca actctaccca gttaatacag aaagaataat tgctattagt cttatggttt
     2461 tgaaaatttt tagtactaga attctagaac cagtctagag aaactctaca acattttagg
     2521 agttctaaaa ttaaataaca tttttacttc ataggttaca tagaagaact agtttataac
     2581 acttataacc tgttactgtt agcattgttt attaaatttg cttcatatag tcatgaaaag
     2641 actaactgat actctgttac tgcaagtgtt ccatttggct tctcttagtg aaagtcactg
     2701 tgcataccca accggatgtt gactgttgaa gacaatatct ggtatctttg ctgtctaagc
     2761 tcaaggtgcc ctgttgagaa ggaaagccat ttcactttta ttgcaggctg gaaatgtctt
     2821 actatcaata agcaaaaaac aaaagtaaac aaacagcgac tagagagata gatggtcaga
     2881 ggctaagaat gcttgctgct cttccaagga cctgagttca gtctccagca cccacatgga
     2941 caacttacaa ccatctgtta actctggttc caggcaatct gatcctctct ggtggcttcc
     3001 ttgggcgcct gcacatatgt gtgcatattg acatgcatac atataaatat aaatagtttc
     3061 aaaaacaaac aaagagcaaa gacttcatga tgttaagcag aaacccagca actacactac
     3121 tgtgtgtgta tacctgtgat aaattaaagc acaaaaactt gcacaccagt gttcacggca
     3181 gcattattca caatagtcaa atgtggaaag atgtccatca cgtgatgcat gtatgaataa
     3241 atgtggtaga ttcagacaat gaaatgtgta ttaaac
//
