LOCUS       NM_008610               3078 bp    mRNA    linear   ROD 04-MAR-2025
DEFINITION  Mus musculus matrix metallopeptidase 2 (Mmp2), mRNA.
ACCESSION   NM_008610
VERSION     NM_008610.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Feng,W., Hong,N., Wu,Y., Huang,J., Zhang,Q., Liu,G., Qian,Z.,
            Chen,Y., Jin,L., Ding,X., Zhao,P., Chen,A.F. and Yu,Y.
  TITLE     Deficiency of Sox7 leads to congenital aortic stenosis via abnormal
            valve remodeling
  JOURNAL   J Mol Cell Cardiol 199, 81-94 (2025)
   PUBMED   39746830
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Janeckova,E., Juarez-Balarezo,J., Tucker,A.S., Matalova,E.,
            Holomkova,K. and Gaete,M.
  TITLE     Metalloproteinases are involved in the regulation of prenatal tooth
            morphogenesis
  JOURNAL   Am J Physiol Cell Physiol 328 (1), C323-C333 (2025)
   PUBMED   39510136
REFERENCE   3  (bases 1 to 3078)
  AUTHORS   Patel,V.N., Aure,M.H., Choi,S.H., Ball,J.R., Lane,E.D., Wang,Z.,
            Xu,Y., Zheng,C., Liu,X., Martin,D., Pailin,J.Y., Prochazkova,M.,
            Kulkarni,A.B., van Kuppevelt,T.H., Ambudkar,I.S., Liu,J. and
            Hoffman,M.P.
  TITLE     Specific 3-O-sulfated heparan sulfate domains regulate salivary
            gland basement membrane metabolism and epithelial differentiation
  JOURNAL   Nat Commun 15 (1), 7584 (2024)
   PUBMED   39217171
  REMARK    Erratum:[Nat Commun. 2024 Oct 10;15(1):8784. doi:
            10.1038/s41467-024-53230-4. PMID: 39389981]
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3078)
  AUTHORS   Li,Y., Zhong,X. and Yang,F.
  TITLE     Silencing HE4 alleviates the renal fibrosis in lupus nephritis mice
            by regulating the C3/MMPs/prss axis
  JOURNAL   Naunyn Schmiedebergs Arch Pharmacol 397 (7), 4823-4831 (2024)
   PUBMED   38157023
  REMARK    GeneRIF: Silencing HE4 alleviates the renal fibrosis in lupus
            nephritis mice by regulating the C3/MMPs/prss axis.
REFERENCE   5  (bases 1 to 3078)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   6  (bases 1 to 3078)
  AUTHORS   Gack,S., Vallon,R., Schmidt,J., Grigoriadis,A., Tuckermann,J.,
            Schenkel,J., Weiher,H., Wagner,E.F. and Angel,P.
  TITLE     Expression of interstitial collagenase during skeletal development
            of the mouse is restricted to osteoblast-like cells and
            hypertrophic chondrocytes
  JOURNAL   Cell Growth Differ 6 (6), 759-767 (1995)
   PUBMED   7669731
REFERENCE   7  (bases 1 to 3078)
  AUTHORS   Reponen,P., Leivo,I., Sahlberg,C., Apte,S.S., Olsen,B.R.,
            Thesleff,I. and Tryggvason,K.
  TITLE     92-kDa type IV collagenase and TIMP-3, but not 72-kDa type IV
            collagenase or TIMP-1 or TIMP-2, are highly expressed during mouse
            embryo implantation
  JOURNAL   Dev Dyn 202 (4), 388-396 (1995)
   PUBMED   7626795
REFERENCE   8  (bases 1 to 3078)
  AUTHORS   Sahlberg,C., Reponen,P., Tryggvason,K. and Thesleff,I.
  TITLE     Association between the expression of murine 72 kDa type IV
            collagenase by odontoblasts and basement membrane degradation
            during mouse tooth development
  JOURNAL   Arch Oral Biol 37 (12), 1021-1030 (1992)
   PUBMED   1471951
REFERENCE   9  (bases 1 to 3078)
  AUTHORS   Reponen,P., Sahlberg,C., Huhtala,P., Hurskainen,T., Thesleff,I. and
            Tryggvason,K.
  TITLE     Molecular cloning of murine 72-kDa type IV collagenase and its
            expression during mouse development
  JOURNAL   J Biol Chem 267 (11), 7856-7862 (1992)
   PUBMED   1373140
REFERENCE   10 (bases 1 to 3078)
  AUTHORS   Brenner,C.A., Adler,R.R., Rappolee,D.A., Pedersen,R.A. and Werb,Z.
  TITLE     Genes for extracellular-matrix-degrading metalloproteinases and
            their inhibitor, TIMP, are expressed during early mammalian
            development
  JOURNAL   Genes Dev 3 (6), 848-859 (1989)
   PUBMED   2744464
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC121604.3, BC070430.1 and
            W53462.1.
            
            On Feb 6, 2016 this sequence version replaced NM_008610.2.
            
            Summary: This gene encodes a member of the matrix metalloproteinase
            family of extracellular matrix-degrading enzymes that are involved
            in tissue remodeling, wound repair, progression of atherosclerosis
            and tumor invasion. The encoded preproprotein undergoes proteolytic
            processing to generate a mature, zinc-dependent endopeptidase
            enzyme that hydrolyzes collagens, gelatins, laminin, fibronectin
            and elastin. Mice lacking the encoded protein exhibit suppressed
            angiogenesis and attenuated features of human multicentric
            osteolysis with arthritis including abnormal skeletal and
            craniofacial development. [provided by RefSeq, Feb 2016].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC070430.1, AK148184.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AC121604.3         120230-120260       c
            32-3072             BC070430.1         1-3041
            3073-3078           W53462.1           398-403
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 44.99 cM"
     gene            1..3078
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /note="matrix metallopeptidase 2"
                     /db_xref="GeneID:17390"
                     /db_xref="MGI:MGI:97009"
     exon            1..481
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             329..2317
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /EC_number="3.4.24.24"
                     /note="72 kDa type IV collagenase; 72 kDa gelatinase;
                     72kDa gelatinase; 72kDa type IV collagenase; matrix
                     metalloproteinase 2; gelatinase A; 72kD gelatinase; 72kD
                     type IV collagenase"
                     /codon_start=1
                     /product="72 kDa type IV collagenase preproprotein"
                     /protein_id="NP_032636.1"
                     /db_xref="CCDS:CCDS22523.1"
                     /db_xref="GeneID:17390"
                     /db_xref="MGI:MGI:97009"
                     /translation="MEARVAWGALAGPLRVLCVLCCLLGRAIAAPSPIIKFPGDVAPK
                     TDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQNTIETMRKPRC
                     GNPDVANYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARALKVWSDVTPLRFS
                     RIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEG
                     QVVRVKYGNADGEYCKFPFLFNGREYSSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPH
                     EALFTMGGNADGQPCKFPFRFQGTSYNSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPE
                     TAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRNDGKVWCATTTNYDDDRKWGFCPD
                     QGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSHDDIKGIQELYGPSPD
                     ADTDTGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPTGP
                     LLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWVYSASTLERGYPKPLTSLGLPPD
                     VQQVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADSWNAIPDNLDAVVD
                     LQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC"
     exon            482..708
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            709..857
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            858..986
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1160
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1334
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1335..1508
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..1664
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1665..1806
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1943
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            1944..2103
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2104..2213
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2214..3073
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3049..3054
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /note="hexamer: AATAAA"
     polyA_site      3072
                     /gene="Mmp2"
                     /gene_synonym="Clg4a; GelA; MMP-2"
                     /note="major polyA site"
ORIGIN      
        1 ctccacccgg tgctgccagc actcttccag cccagccggc cacatctggc gtctgcccgc
       61 ccttgtttcc gctgcatcca gacttccctg gtggctggag gctctgtgtg catccaggag
      121 tttagatata caaagggatt gccaggacct gcaagcaccc gcggcagtgg tgtgtattgg
      181 gacgtgggac cccgttatga gctcctgagc cccgagaagc agaggcagta gagtaagggg
      241 atcgccgtgc agggcaggcg ccagccgggc ggaccccagg gcacagccag agacctcagg
      301 gtgacacgcg gagcccggga gcgcaacgat ggaggcacga gtggcctggg gagcgctggc
      361 cggacctctg cgggttctct gcgtcctgtg ctgcctgttg ggccgcgcca tcgctgcacc
      421 atcgcccatc atcaagttcc ccggcgatgt cgcccctaaa acagacaaag agttggcagt
      481 gcaatacctg aacactttct atggctgccc caaggagagt tgcaacctct ttgtgctgaa
      541 agataccctc aagaagatgc agaagttctt tgggctgccc cagacaggtg accttgacca
      601 gaacaccatc gagaccatgc ggaagccaag atgtggcaac ccagatgtgg ccaactacaa
      661 cttcttcccc cgcaagccca agtgggacaa gaaccagatc acatacagga tcattggtta
      721 cacacctgac ctggaccctg aaaccgtgga tgatgctttt gctcgggcct taaaagtatg
      781 gagcgacgtc actccgctgc gcttttctcg aatccatgat ggggaggctg acatcatgat
      841 caactttgga cgctgggagc atggagatgg atacccattt gatggcaagg atggactcct
      901 ggcacatgcc tttgccccgg gcactggtgt tgggggagat tctcactttg atgatgatga
      961 gctgtggacc ctgggagaag gacaagtggt ccgcgtaaag tatgggaacg ctgatggcga
     1021 gtactgcaag ttccccttcc tgttcaacgg tcgggaatac agcagctgta cagacactgg
     1081 tcgcagtgat ggcttcctct ggtgctccac cacatacaac tttgagaagg atggcaagta
     1141 tggcttctgc ccccatgaag ccttgtttac catgggtggc aatgcagatg gacagccctg
     1201 caagttcccg ttccgcttcc agggcacctc ctacaacagc tgtaccaccg agggccgcac
     1261 cgatggctac cgctggtgtg gcaccaccga ggactatgac cgggataaga agtatggatt
     1321 ctgtcccgag accgctatgt ccactgtggg tggaaattca gaaggtgccc catgtgtctt
     1381 ccccttcact ttcctgggca acaagtatga gagctgcacc agcgccggcc gcaacgatgg
     1441 caaggtgtgg tgtgcgacca caaccaacta cgatgatgac cggaagtggg gcttctgtcc
     1501 tgaccaagga tatagcctat tcctcgtggc agcccatgag ttcggccatg ccatggggct
     1561 ggaacactct caggaccctg gagctctgat ggccccgatc tacacctaca ccaagaactt
     1621 ccgattatcc catgatgaca tcaaggggat ccaggagctc tatgggccct cccccgatgc
     1681 tgatactgac actggtactg gccccacacc aacactggga cctgtcactc cggagatctg
     1741 caaacaggac attgtctttg atggcatcgc tcagatccgt ggtgagatct tcttcttcaa
     1801 ggaccggttt atttggcgga cagtgacacc acgtgacaag cccacaggtc ccttgctggt
     1861 ggccacattc tggcctgagc tcccagaaaa gattgacgct gtgtatgagg ccccacagga
     1921 ggagaaggct gtgttcttcg cagggaatga gtactgggtc tattctgcta gtactctgga
     1981 gcgaggatac cccaagccac tgaccagcct ggggttgccc cctgatgtcc agcaagtaga
     2041 tgctgccttt aactggagta agaacaagaa gacatacatc tttgcaggag acaagttctg
     2101 gagatacaat gaagtgaaga agaaaatgga ccccggtttc cctaagctca tcgcagactc
     2161 ctggaatgcc atccctgata acctggatgc cgtcgtggac ctgcagggtg gtggtcatag
     2221 ctacttcttc aagggtgctt attacctgaa gctggagaac caaagtctca agagcgtgaa
     2281 gtttggaagc atcaaatcag actggctggg ctgctgagct ggccctgttc ccacgggccc
     2341 tatcatcttc atcgctgcac accaggtgaa ggatgtgaag cagcctggcg gctctgtcct
     2401 cctctgtagt taaccagcct tctccttcac ctggtgactt cagatttaag agggtggctt
     2461 ctttttgtgc ccaaagaaag gtgctgactg taccctcccg ggtgctgctt ctccttcctg
     2521 cccaccctag gggatgcttg gatatttgca atgcagccct cctctgggct gccctggtgc
     2581 tccactcttc tggttcttca acatctatga cctttttatg gctttcagca ctctcagagt
     2641 taatagagac tggcttagga gggcactggt ggccctgtta acagcctggc atggggcagt
     2701 ggggtacagg tgtgccaagg tggaaatcag agacacctgg tttcaccctt tctgctgccc
     2761 agacacctgc accaccttaa ctgttgcttt tgtatgccct tcgctcgttt ccttcaacct
     2821 tttcagtttt ccactccact gcatttcctg cccaaaggac tcgggttgtc tgacatcgct
     2881 gcatgatgca tctcagcccg cctagtgatg gttcccctcc tcactctgtg cagatcatgc
     2941 ccagtcactt cctccactgg atggaggaga accaagtcag tggcttcctg ctcagccttc
     3001 ttgcttctcc ctttaacagt tccccatggg aaatggcaaa caagtataaa taaagacacc
     3061 cattgagtga cagaaaaa
//
