LOCUS       NM_013783               2948 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus membrane metallo-endopeptidase-like 1 (Mmel1), mRNA.
ACCESSION   NM_013783
VERSION     NM_013783.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Chen,Q., Wu,Y., Yu,Y., Wei,J. and Huang,W.
  TITLE     Rho-kinase inhibitor hydroxyfasudil protects against HIV-1
            Tat-induced dysfunction of tight junction and neprilysin/Abeta
            transfer receptor expression in mouse brain microvessels
  JOURNAL   Mol Cell Biochem 476 (5), 2159-2170 (2021)
   PUBMED   33548010
  REMARK    GeneRIF: Rho-kinase inhibitor hydroxyfasudil protects against HIV-1
            Tat-induced dysfunction of tight junction and neprilysin/Abeta
            transfer receptor expression in mouse brain microvessels.
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Karoor,V., Fini,M.A., Loomis,Z., Sullivan,T., Hersh,L.B.,
            Gerasimovskaya,E., Irwin,D. and Dempsey,E.C.
  TITLE     Sustained Activation of Rho GTPases Promotes a Synthetic Pulmonary
            Artery Smooth Muscle Cell Phenotype in Neprilysin Null Mice
  JOURNAL   Arterioscler Thromb Vasc Biol 38 (1), 154-163 (2018)
   PUBMED   29191928
  REMARK    GeneRIF: Sustained Rho activation in NEP(-/-) pulmonary artery
            smooth muscle cells is associated with a decrease in SM-protein
            levels and increased migration and proliferation.
REFERENCE   3  (bases 1 to 2948)
  AUTHORS   Wick,M.J., Loomis,Z.L., Harral,J.W., Le,M., Wehling,C.A.,
            Miller,Y.E. and Dempsey,E.C.
  TITLE     Protection against vascular leak in neprilysin transgenic mice with
            complex overexpression pattern
  JOURNAL   Transgenic Res 25 (6), 773-784 (2016)
   PUBMED   27369050
  REMARK    GeneRIF: These studies help to characterize our complex transgenic
            model of NEP overexpression and further demonstrate NEP's
            protective effects against plasma extravasation.
REFERENCE   4  (bases 1 to 2948)
  AUTHORS   Krohn,M., Bracke,A., Avchalumov,Y., Schumacher,T., Hofrichter,J.,
            Paarmann,K., Frohlich,C., Lange,C., Bruning,T., von Bohlen Und
            Halbach,O. and Pahnke,J.
  TITLE     Accumulation of murine amyloid-beta mimics early Alzheimer's
            disease
  JOURNAL   Brain 138 (Pt 8), 2370-2382 (2015)
   PUBMED   25991605
  REMARK    GeneRIF: neprilysin x ABCC1 double-deficient mice present a new
            model for early effects of amyloid-beta-related mild cognitive
            impairment that allows investigations without artificial
            overexpression of inherited Alzheimer's disease genes
REFERENCE   5  (bases 1 to 2948)
  AUTHORS   Rossier,J., Bernard,A., Cabungcal,J.H., Perrenoud,Q., Savoye,A.,
            Gallopin,T., Hawrylycz,M., Cuenod,M., Do,K., Urban,A. and Lein,E.S.
  TITLE     Cortical fast-spiking parvalbumin interneurons enwrapped in the
            perineuronal net express the metallopeptidases Adamts8, Adamts15
            and Neprilysin
  JOURNAL   Mol Psychiatry 20 (2), 154-161 (2015)
   PUBMED   25510509
  REMARK    GeneRIF: This study demonstrated that Cortical fast-spiking
            parvalbumin interneurons enwrapped in the perineuronal net express
            the Neprilysin in mice.
REFERENCE   6  (bases 1 to 2948)
  AUTHORS   Voisin,S. and Ouimet,T.
  TITLE     The ultimate tryptophan residue of neprilysin 2 is not involved in
            protein maturation and enzymatic activity
  JOURNAL   Biochem Biophys Res Commun 335 (2), 356-360 (2005)
   PUBMED   16081046
  REMARK    GeneRIF: Modification of the structure of the C-terminal region
            will either impair substrate hydrolysis or completely abolish the
            secretion and enzymatic activity of the secreted isoform of rat
            NEP2.
REFERENCE   7  (bases 1 to 2948)
  AUTHORS   Carpentier,M., Guillemette,C., Bailey,J.L., Boileau,G.,
            Jeannotte,L., DesGroseillers,L. and Charron,J.
  TITLE     Reduced fertility in male mice deficient in the zinc
            metallopeptidase NL1
  JOURNAL   Mol Cell Biol 24 (10), 4428-4437 (2004)
   PUBMED   15121861
  REMARK    GeneRIF: One of the roles of NL1 in mice is related to sperm
            function. NL1 modulates the processes of fertilization and early
            embryonic development in vivo.
REFERENCE   8  (bases 1 to 2948)
  AUTHORS   Shirotani,K., Tsubuki,S., Iwata,N., Takaki,Y., Harigaya,W.,
            Maruyama,K., Kiryu-Seo,S., Kiyama,H., Iwata,H., Tomita,T.,
            Iwatsubo,T. and Saido,T.C.
  TITLE     Neprilysin degrades both amyloid beta peptides 1-40 and 1-42 most
            rapidly and efficiently among thiorphan- and
            phosphoramidon-sensitive endopeptidases
  JOURNAL   J Biol Chem 276 (24), 21895-21901 (2001)
   PUBMED   11278416
REFERENCE   9  (bases 1 to 2948)
  AUTHORS   Ghaddar,G., Ruchon,A.F., Carpentier,M., Marcinkiewicz,M.,
            Seidah,N.G., Crine,P., Desgroseillers,L. and Boileau,G.
  TITLE     Molecular cloning and biochemical characterization of a new mouse
            testis soluble-zinc-metallopeptidase of the neprilysin family
  JOURNAL   Biochem J 347 (Pt 2), 419-429 (2000)
   PUBMED   10749671
REFERENCE   10 (bases 1 to 2948)
  AUTHORS   Ikeda,K., Emoto,N., Raharjo,S.B., Nurhantari,Y., Saiki,K.,
            Yokoyama,M. and Matsuo,M.
  TITLE     Molecular identification and characterization of novel
            membrane-bound metalloprotease, the soluble secreted form of which
            hydrolyzes a variety of vasoactive peptides
  JOURNAL   J Biol Chem 274 (45), 32469-32477 (1999)
   PUBMED   10542292
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF176569.1, BY105444.1, AF302076.1 and AF157105.1.
            
            On Oct 31, 2007 this sequence version replaced NM_013783.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF176569.1, AF157105.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-41                AF176569.1         1-41
            42-236              BY105444.1         1-195
            237-262             AF176569.1         237-262
            263-412             BY105444.1         222-371
            413-2648            AF302076.1         103-2338
            2649-2928           AF176569.1         2646-2925
            2929-2948           AF157105.1         2873-2892
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 85.54 cM"
     gene            1..2948
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /note="membrane metallo-endopeptidase-like 1"
                     /db_xref="GeneID:27390"
                     /db_xref="MGI:MGI:1351603"
     exon            1..140
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            141..455
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            456..524
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            525..584
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            585..746
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            747..827
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            828..923
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            924..1042
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1108
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1109..1243
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1244..1333
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1470
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1471..1564
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1565..1693
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1694..1792
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1793..1876
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1877..1980
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            1981..2039
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            2040..2159
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            2160..2293
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            2294..2359
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            2360..2455
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            2456..2532
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     exon            2533..2937
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2917..2922
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /note="hexamer: AGTAAA"
     polyA_site      2936
                     /gene="Mmel1"
                     /gene_synonym="Mell1; NEP2; NEP2(m); NEPII; NL-1; Nl1;
                     NL2; SEP"
                     /note="major polyA site"
ORIGIN      
        1 cgggagccag caccgtgtga cctcacaccc agctcagctg ctctactcca cccggagccc
       61 accttggcca gctcacccca gctctgagac atcccaacct agcctttaag gacttgccta
      121 gcagtgactg agagcaccag ggtcccctgg gcacttgggg cacagcttac agcattgaga
      181 gcagagacca ggacagtgca ccagcttcag tgtgtcctag gcatccgatc cgggctccag
      241 ctgcctctct cctagccctg gcttgggggg cttagcggtg tgccttccac ccagaaccgg
      301 ctgataggga aagtctgaga gcccagtggg gatggtggag agagcaggct ggtgtcggaa
      361 gaagtcccca ggcttcgtgg agtatgggct gatggtgctg ctgctgctgc tgttgctggg
      421 agccatagtg actctgggtg tcttctacag catagggaag cagctgcccc tcttaactag
      481 cctgctacac ttctcctggg atgagaggac ggttgtaaaa cgagccctca gggattcatc
      541 actgaaaagt gacatctgca ccaccccaag ctgtgtgata gcagctgcca gaatcctcga
      601 aaacatggac caatcgagga acccctgtga aaacttctac cagtacgctt gcggaggctg
      661 gctgaggcac cacgtgatcc cagagaccaa ctccagatac agcgtctttg acatccttcg
      721 ggacgagctg gaggttatcc tcaaaggggt gctggaggat tccacttccc agcatcgccc
      781 ggccgtggag aaggccaaga cactatatcg ctcctgcatg aaccaaagtg tgatcgagaa
      841 gagagactct gagcccctgc tgagcgtctt aaaaatggta ggaggttggc ctgtggccat
      901 ggataagtgg aacgagacca tgggcctcaa gtgggaactg gagcgacagt tggctgtgtt
      961 gaactcgcag ttcaacaggc gggtcctcat cgacctcttc atctggaatg acgaccagaa
     1021 ctccagccgg catgtcatct acatagacca gcccaccttg ggcatgccat cccgggagta
     1081 ctatttccag gaggacaaca accacaaggt acggaaagcc tacctggagt tcatgacgtc
     1141 agtggccact atgcttagga aagaccagaa cctgtccaag gagagcgcca tggtgcggga
     1201 ggagatggcg gaggtgctgg aactggagac gcatctggcc aacgccacag tcccccagga
     1261 gaaaaggcat gatgtcactg ccctgtacca ccgaatggac ctgatggagc tacaggaaag
     1321 gtttggtctg aaggggttta actggactct cttcatacaa aacgtgttgt cttctgtgga
     1381 agtcgagctg ttcccagatg aggaggtggt ggtctacggc atcccctacc tggagaatct
     1441 ggaggatatc attgatagct actcagcacg gaccatgcag aactacctgg tatggcgcct
     1501 ggtgctagat cgaattggca gcctgagcca gagattcaaa gaggcgcgtg tggactaccg
     1561 caaggcgctg tacggcacga ccgtggagga ggtacgctgg cgagagtgtg tcagctatgt
     1621 caacagtaac atggagagcg ccgtgggctc cctctacatc aagcgggcct tctccaagga
     1681 cagcaagagc acggtcagag agctgattga gaagataagg tccgtgtttg tggataacct
     1741 ggatgagctg aactggatgg acgaggaatc caagaagaag gcccaggaaa aggccatgaa
     1801 tatacgggaa cagattggct accctgacta cattttggaa gataacaata aacacctgga
     1861 tgaggaatac tccagtttga ctttctatga ggacctgtat tttgagaacg gacttcagaa
     1921 cctcaagaac aatgcccaga ggagcctcaa gaagcttcgg gaaaaggtgg accagaatct
     1981 ctggatcatc ggggctgcag tggtcaatgc attctactcc ccaaacagaa accagatcgt
     2041 ctttccagca gggattctcc agccgccctt cttcagcaag gaccaaccac agtccttgaa
     2101 ttttgggggc atcgggatgg tgattgggca cgagatcaca cacggctttg atgataatgg
     2161 tcgtaacttt gacaagaacg gcaacatgct ggactggtgg agtaacttct cggcccggca
     2221 cttccaacag cagtcgcaat gcatgatcta tcagtacggc aacttctctt gggaactagc
     2281 agacaaccag aatgtgaacg gattcagtac cctcggggag aacattgccg acaacggagg
     2341 tgtgcgacag gcatacaagg cttacctacg gtggctggct gatggcggca aagatcagcg
     2401 actgccggga ctgaacctga cctatgccca gcttttcttc atcaactatg cccaggtgtg
     2461 gtgtgggtcc tataggccgg agttcgccgt ccagtccatc aagacggacg tccacagtcc
     2521 tcttaagtac agggtgctgg gctcactaca gaacctgcca ggcttctctg aggcattcca
     2581 ctgcccacga ggcagcccca tgcaccccat gaagcgatgt cgcatctggt agccaaggct
     2641 gagctatgct gcggcccacg ccccgccacc cagaggcttc gcgaatggtg tagctggcag
     2701 agatgtgcag gtctttgcct gaaggccacc ggagccacca gccagccctc cgcgcccagc
     2761 ctagagtgta gccacccgcc cacacccggg atgagtggtg ccggtcctgc gccccctcag
     2821 gccagtgagg gtcagcagcc caggaagagc agtcagctgc cttccaccct ctccatagtg
     2881 tgtggctaaa tgttctcgag cttcagactt gagctaagta aacgcttcaa agaagacaaa
     2941 aaaaaaaa
//
