LOCUS       NM_001357335            6049 bp    mRNA    linear   ROD 04-OCT-2024
DEFINITION  Mus musculus membrane metallo endopeptidase (Mme), transcript
            variant 4, mRNA.
ACCESSION   NM_001357335 XM_006501095
VERSION     NM_001357335.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 6049)
  AUTHORS   Erin,N. and Akdeniz,O.
  TITLE     ADAM10 and Neprilysin level decreases in immune cells of mice
            bearing metastatic breast carcinoma: Possible role in cancer
            inflammatory response
  JOURNAL   Int Immunopharmacol 127, 111384 (2024)
   PUBMED   38141405
  REMARK    GeneRIF: ADAM10 and Neprilysin level decreases in immune cells of
            mice bearing metastatic breast carcinoma: Possible role in cancer
            inflammatory response.
REFERENCE   3  (bases 1 to 6049)
  AUTHORS   Esser,N., Mongovin,S.M., Mundinger,T.O., Barrow,B.M. and Zraika,S.
  TITLE     Neprilysin deficiency reduces hepatic gluconeogenesis in high
            fat-fed mice
  JOURNAL   Peptides 168, 171076 (2023)
   PUBMED   37572792
  REMARK    GeneRIF: Neprilysin deficiency reduces hepatic gluconeogenesis in
            high fat-fed mice.
REFERENCE   4  (bases 1 to 6049)
  AUTHORS   Chen,Y. and Burnett,J.C. Jr.
  TITLE     Biochemistry, Therapeutics, and Biomarker Implications of
            Neprilysin in Cardiorenal Disease
  JOURNAL   Clin Chem 63 (1), 108-115 (2017)
   PUBMED   28062615
  REMARK    Review article
REFERENCE   5  (bases 1 to 6049)
  AUTHORS   Bayes-Genis,A., Barallat,J. and Richards,A.M.
  TITLE     A Test in Context: Neprilysin: Function, Inhibition, and Biomarker
  JOURNAL   J Am Coll Cardiol 68 (6), 639-653 (2016)
   PUBMED   27491909
  REMARK    Review article
REFERENCE   6  (bases 1 to 6049)
  AUTHORS   Turner,A.J. and Nalivaeva,N.N.
  TITLE     Proteinase dysbalance in pathology: the neprilysin (NEP) and
            angiotensin-converting enzyme (ACE) families
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 52 (4), 40-48 (2006)
   PUBMED   17543197
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 6049)
  AUTHORS   Weil,M., Itin,A. and Keshet,E.
  TITLE     A role for mesenchyme-derived tachykinins in tooth and mammary
            gland morphogenesis
  JOURNAL   Development 121 (8), 2419-2428 (1995)
   PUBMED   7545574
REFERENCE   8  (bases 1 to 6049)
  AUTHORS   Kalled,S.L., Siva,N., Stein,H. and Reinherz,E.L.
  TITLE     The distribution of CD10 (NEP 24.11, CALLA) in humans and mice is
            similar in non-lymphoid organs but differs within the hematopoietic
            system: absence on murine T and B lymphoid progenitors
  JOURNAL   Eur J Immunol 25 (3), 677-687 (1995)
   PUBMED   7705396
REFERENCE   9  (bases 1 to 6049)
  AUTHORS   Watson,M.L., Rao,J.K., Gilkeson,G.S., Ruiz,P., Eicher,E.M.,
            Pisetsky,D.S., Matsuzawa,A., Rochelle,J.M. and Seldin,M.F.
  TITLE     Genetic analysis of MRL-lpr mice: relationship of the Fas apoptosis
            gene to disease manifestations and renal disease-modifying loci
  JOURNAL   J Exp Med 176 (6), 1645-1656 (1992)
   PUBMED   1460423
REFERENCE   10 (bases 1 to 6049)
  AUTHORS   Chen,C.Y., Salles,G., Seldin,M.F., Kister,A.E., Reinherz,E.L. and
            Shipp,M.A.
  TITLE     Murine common acute lymphoblastic leukemia antigen (CD10 neutral
            endopeptidase 24.11). Molecular characterization, chromosomal
            localization, and modeling of the active site
  JOURNAL   J Immunol 148 (9), 2817-2825 (1992)
   PUBMED   1374101
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121993.2 and AC121840.3.
            
            On Oct 26, 2017 this sequence version replaced XM_006501095.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR10223971.3847.1,
                                           SRR10223969.3733.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849380
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-405               AC121993.2         36685-37089         c
            406-575             AC121993.2         32006-32175         c
            576-611             AC121993.2         31286-31321         c
            612-773             AC121993.2         5425-5586           c
            774-854             AC121993.2         4435-4515           c
            855-950             AC121993.2         4233-4328           c
            951-1069            AC121993.2         4012-4130           c
            1070-1135           AC121993.2         2585-2650           c
            1136-1270           AC121840.3         164938-165072       c
            1271-1372           AC121840.3         163080-163181       c
            1373-1509           AC121840.3         161455-161591       c
            1510-1603           AC121840.3         161253-161346       c
            1604-1732           AC121840.3         159835-159963       c
            1733-1831           AC121840.3         158936-159034       c
            1832-1912           AC121840.3         157871-157951       c
            1913-2016           AC121840.3         156367-156470       c
            2017-2075           AC121840.3         146124-146182       c
            2076-2195           AC121840.3         143037-143156       c
            2196-2329           AC121840.3         140193-140326       c
            2330-2395           AC121840.3         140026-140091       c
            2396-2491           AC121840.3         136514-136609       c
            2492-2568           AC121840.3         136132-136208       c
            2569-6049           AC121840.3         121375-124855       c
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 29.97 cM"
     gene            1..6049
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /note="membrane metallo endopeptidase"
                     /db_xref="GeneID:17380"
                     /db_xref="MGI:MGI:97004"
     exon            1..405
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            406..575
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     CDS             416..2668
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /EC_number="3.4.24.11"
                     /note="common acute lymphoblastic leukemia antigen;
                     enkephalinase; atriopeptidase; neutral endopeptidase
                     24.11; skin fibroblast elastase"
                     /codon_start=1
                     /product="neprilysin"
                     /protein_id="NP_001344264.1"
                     /db_xref="CCDS:CCDS17381.1"
                     /db_xref="GeneID:17380"
                     /db_xref="MGI:MGI:97004"
                     /translation="MGRSESQMDITDINAPKPKKKQRWTPLEISLSVLVLLLTIIAVT
                     MIALYATYDDGICKSSDCIKSAARLIQNMDASVEPCTDFFKYACGGWLKRNVIPETSS
                     RYSNFDILRDELEVILKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGQPLLKL
                     LPDIYGWPVASDNWDQTYGTSWTAEKSIAQLNSKYGKKVLINFFVGTDDKNSTQHIIH
                     FDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEQSLPIDENQLSLEMNKV
                     MELEKEIANATTKPEDRNDPMLLYNKMTLAKLQNNFSLEVNGKSFSWSNFTNEIMSTV
                     NINIQNEEEVVVYAPEYLTKLKPILTKYSPRDLQNLMSWRFIMDLVSSLSRNYKESRN
                     AFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREV
                     FIQTLDDLTWMDAETKKKAEEKALAIKERIGYPDDIISNENKLNNEYLELNYREDEYF
                     ENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFS
                     AQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSANNFKDQSQCMVY
                     QYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNH
                     KQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFADAFHCRKNSY
                     MNPERKCRVW"
     exon            576..611
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            612..773
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            774..854
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            855..950
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            951..1069
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1135
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1136..1270
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1271..1372
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..1509
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1510..1603
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1604..1732
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..1831
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1912
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            1913..2016
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2017..2075
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2076..2195
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2196..2329
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2330..2395
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2396..2491
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2492..2568
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     exon            2569..6049
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3734..3739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /note="hexamer: AATAAA"
     polyA_site      3759
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /note="major polyA site"
     polyA_site      5320
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /note="major polyA site"
     regulatory      6029..6034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /note="hexamer: ATTATA"
     polyA_site      6049
                     /gene="Mme"
                     /gene_synonym="6030454K05Rik; CALLA; CD10; NEP; SFE"
                     /note="major polyA site"
ORIGIN      
        1 gagggaaaac ggagagagca agagtatgtg agagagaagc attctggtct tagtcagtta
       61 gagaatgaga gcctgcaatc agcactacac aatcccagag agacagtgta ttgttgtctt
      121 gaaagccaca aagctaaaca attccaagaa tgtctggcga agcccttttg ggaagggcaa
      181 ggtgggggac gaactgggag agggagcccc ctccgcccct ttacacggtg tgacaggatt
      241 tgggagacct ggcggaaacc ttcaggtcac ccagggaact gctctcgcgg ctggtcctgt
      301 gcggcgaggc ggggggtgag cgcgaggcgc gcagggcgcg gagatgtgca agtggaggag
      361 ctggacggag tgcaggcgta agctgcagag cggctaaggc gagggatttt aggtgatggg
      421 aagatcagaa agtcagatgg atataactga tatcaatgct ccaaagccaa agaagaaaca
      481 gcgatggact cccctggaga tcagcctctc tgtgcttgtc ttgctcctga ctatcatagc
      541 ggtgacaatg atcgctctct atgcaaccta tgatgatggc atttgcaaat catcagactg
      601 cataaaatca gctgctcgac tgattcagaa tatggatgcc tctgttgagc catgtacaga
      661 cttcttcaag tatgcttgtg gaggctggtt gaaacgcaac gtcattcccg agaccagttc
      721 ccgatacagt aattttgaca ttttaagaga tgagctagaa gtcattttga aagatgtcct
      781 tcaagaaccc aaaactgaag acatagtagc agtgcagaaa gcaaaaacct tgtacagatc
      841 gtgtataaat gagtctgcta ttgatagcag aggcggacaa cctctactca aactgttacc
      901 agatatatat gggtggccag tagcatcaga taactgggat caaacatatg gcacttcttg
      961 gacagctgag aaatccattg cacaactgaa ttctaaatat gggaaaaagg tcctcattaa
     1021 tttttttgtt ggcactgatg ataagaattc tacccagcat ataattcatt ttgaccagcc
     1081 tcgacttggc ctcccttcca gagactacta tgagtgcact ggaatatata aagaggcttg
     1141 cacagcatat gtggatttta tgatttctgt ggccagactg attcgtcagg aacaaagttt
     1201 gcctattgat gaaaaccagc tctctttgga aatgaataaa gttatggaat tggaaaaaga
     1261 aattgccaat gctacaacta aaccagaaga ccgaaatgac ccaatgctgc tttataacaa
     1321 aatgacattg gccaagctgc agaacaactt ttccctggag gtcaatggga agtcattcag
     1381 ctggtcaaat ttcacaaatg aaatcatgtc aactgtgaat attaatattc aaaatgagga
     1441 agaagttgtt gtctatgctc cagaatattt aaccaaactt aagcctattc ttaccaaata
     1501 ttctcccaga gatcttcaaa atttaatgtc ctggaggttc ataatggatc ttgtaagcag
     1561 cctcagccga aactacaagg agtccagaaa tgctttccgc aaggcccttt atgggactac
     1621 atcagaaact gcaacatgga gacggtgtgc taactacgtc aatgggaata tggagaatgc
     1681 tgtggggagg ctttatgtgg aagcggcttt tgctggagag agcaagcacg tggttgaaga
     1741 cttgattgca caaatccggg aagtttttat tcagactttg gatgacctta cttggatgga
     1801 tgctgagaca aaaaagaaag ctgaagaaaa ggccttggca attaaagaaa ggattggcta
     1861 tcctgatgac atcatttcca atgagaacaa actgaataat gagtatcttg agttgaacta
     1921 cagggaagat gaatactttg agaacataat tcaaaatttg aaattcagcc aaagcaagca
     1981 gctaaagaag ctccgagaaa aagtggacaa agatgagtgg ataagtggag cagctgtagt
     2041 caatgcattt tattcctcag gccgaaatca gatagtcttc cccgctggca ttttgcagcc
     2101 cccattcttc agtgctcagc aatccaactc attgaactat gggggcatcg gcatggtcat
     2161 tggacatgaa atcacacatg gatttgatga caatggaaga aatttcaata aagatggaga
     2221 cctcgttgac tggtggacgc agcagtcggc aaataatttt aaagaccaat cccagtgtat
     2281 ggtataccag tatggaaact tttcctggga cctagcaggt ggacagcatc tcaatggaat
     2341 taatacacta ggagaaaaca ttgctgataa tggaggtatt ggccaagcat acagagccta
     2401 tcagaattat gttaaaaaga atggtgaaga aaaattactc cctggacttg acctcaatca
     2461 caaacaacta tttttcctga actttgccca ggtgtggtgt gggacctacc ggccagagta
     2521 tgcagtcaat tctattaaaa cagatgtaca cagtcctgga aatttcagga tcattgggac
     2581 tttgcagaac tctgccgagt ttgcagatgc ctttcattgc cgcaagaatt catacatgaa
     2641 tccagaaagg aaatgtcggg tttggtgatc ttcacaagat actgaacatc cacggcaaga
     2701 tttgccaaag ccacggaaac aggaactctt ccctcagaga aactgggccc cggaagtttc
     2761 ttcagcttcc tggggggcaa ttcacagaga cgagcatgag ctaacataaa tgaaattaga
     2821 ttattaaatc tgctgtgaat gaaaggggag ggagcctacg atctagcaaa tcaatcactt
     2881 cactgtgtaa ataatgatta ctttccaacg gtagtactac cgttcacttc tgtttctcac
     2941 acagactgca gctttcatgt tgtctgtaga gaacagtgtt aacacttaaa gcagactatg
     3001 acttctgatc aaaaggagga cgatgctcag tacagcaggg caccaaagca cagatttgcc
     3061 cctcagcact cacttttgtt tgcaacattc atgctccttc caaactctcc caaagaactc
     3121 ccatacagac tgtggccttc aggctcgtgc agttttgaac tcattttacc atacataagt
     3181 tattcattca tcccacatca atttagtttg aacactctta gagcttaaac tagagtatct
     3241 gaaacggttc ccctatttca cccactttag tgatgttgag attcttgaac ccccctgcag
     3301 atctttgagc aatttcttgc tcttgctgcc cctcagactc agtcttttaa aggatttgta
     3361 gtaatgtata aagaataatt ctatatttaa ttattaacta catttatgac tgaataaacc
     3421 attgctatag gtaatcattg aatattgaag ttatatggcc aagatagttt tgaagatctg
     3481 taacatgata tacagacgaa aatttgagac tctttaaatc tgtaaatcat attgatgaaa
     3541 agctttaagt acaaactctg ggggtgagca ttaccattgg acgggtgtct tgagatacat
     3601 aatagttgtg gtttacaaac agcattttca agattaagtc tgtccctagt ggctaagaaa
     3661 gggtctaact gtatctgtgt tgctccatga atgcttagcc ttcaggtttg tcatcagatg
     3721 aaaatatttt gataataaat tgacgttgtg agccccttac taggcctgtg tgcacgccat
     3781 gccacctagc tttagaaagc ctatgtctga atcgagatgg gcagcctcca ctgtacctgc
     3841 ttcttgaatt ctacagctca tggactctaa ttggatttcc tgaggcactc atatgccttc
     3901 cttgtccttc tgcttacttt agtgattttc actattcagt ataaatttta caaagattca
     3961 tttctacagt ccttattttt atcaaaaggt gaaaatgaaa acatttctat ccaaactctt
     4021 agttttcatg ccttgtgaga aaagcccact tgcttgtaaa agtgttacag tacaatatgt
     4081 aaaattgtat agaacaaaaa ctacagttca gcgaactgat attctctgaa cacagcaccc
     4141 tagctaaaat ctataagaaa tggaacactg tagctttctg ccatcatcat ataactgcta
     4201 ggcatacatt atccagatct ccagcattat gcactagctc tgccccattt tgtctttcag
     4261 tcatgcatta cttcctatct tacttctctc tgccttacat ttgtaaaatt tagctgtatg
     4321 attggtttat atgtgaatct atcattgttg aataaggttc tgttgtgtga ctatactaca
     4381 aatattccat cttattattg atggactttt aaggactcca tgaactcctg tctattctgg
     4441 ttttggattg ttacaaatga tataactatg cattatgaac tgtgcttata tattcacctt
     4501 tacttgaatt atcattggaa tcactttccc aaacctatcc aaaactatca taaattttaa
     4561 agacacggtt tcagactgtg tggttacaaa catccttatc tttttccctt ttaaaacaaa
     4621 cactattgtc atgaataata cttatatgac tgtttctttg aaatcaattt aaacatactt
     4681 gagtactatg aatggtggta gtagaagatt tcagttaaac agctatctct gggttatttt
     4741 catttatgtc ctgtcatagc agccaaagag acagaagtga agagtcaatg gccactagca
     4801 tctccactgg tagttcatta aaggatcaag cttccaaacc tatgtaacag caactctggt
     4861 gcctagattc ctgctgtata tacagcatac agcatattct ttgagctaag ggattttaac
     4921 ataaaagtta aaatggtaat tttttcccta ttactcgggc atccagatat cagtggttgt
     4981 agctggtact cagaacccta tgttctttct taattcctac cagccatttg tcacattact
     5041 taagagttac atataatatt atacataaat ttgcttccac cgtatttaag ttctgatcgg
     5101 ggagagtttg ttgtacactt gagagctgtt gtagctcatc agtgaagatg aagctcctgg
     5161 cagtatgtgt tggcaaggag tctgcttcca taacatagtg tctgtgtgca aggcaaaagg
     5221 atgctcccca ggaagttgag gatttgttct agttctactg ccaaagtgac cttgggcaca
     5281 tgtatatccc ttggggcttc tgcctctttt actccaaaaa gaagacagta agattccagc
     5341 agttcatacc aatgctgcct tccctttaac ctgttaccca ggaaacaagg accaaaattc
     5401 taacaagctt ggggctgtga tactgttact attaggcatg atgataggac agacacttgc
     5461 aaactttctt aagtgttcat aattcctata gcaatagatg gagctcttag agtagaaggc
     5521 atctgtcagt ttagaaagaa aataacattc taagattcta ctgtgtcaaa agaagtatat
     5581 tcagaatttt taaaattagg tcctattctc taggatagaa aataacaaac attttcataa
     5641 tgttgtttct atgactagtt gactgttcca atttcaccaa tatgcatgta aacatacaca
     5701 caccatttat gccttgaaaa atattaagat aaaataggac attgttttct atgcagtctt
     5761 atatttcctt catagtttca aactaaatct ttttaaacaa ataatatgaa gtattttacc
     5821 aacattttat tttaaagata accagttttc tgattaggtg tttcatatgg ctaccaaatg
     5881 ttatcaggag tcacaattat aattggatta tgctgaagcc aaatgtgcaa atgtgaagag
     5941 ctggaccact caggtgactt ggtgacagag gagccacatg tgactgttgt caaaatagca
     6001 tgtttattgc attctaactt tttctctaat tatattttct tagtgtcaa
//
